The Investigation of Caspase-3 and Tumor Necrosis Factor-Alpha Expression in Placentas of Patients with Preterm Premature Rupture of Membranes

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dc.contributor.authorErmis, Isilay Sezen
dc.contributor.authorAsir, Firat
dc.contributor.authorOglak, Suleyman Cemil
dc.contributor.authorKaplan, Ozge
dc.contributor.authorAydeniz, Gul Ebru
dc.contributor.authorDeveci, Engin
dc.date.accessioned2024-04-24T17:20:24Z
dc.date.available2024-04-24T17:20:24Z
dc.date.issued2023
dc.departmentDicle Üniversitesien_US
dc.description.abstractBackground: Caspase-3 is involved in the execution of apoptosis and is widely used as an apoptotic marker. Tumor necrosis factor-& alpha; (TNF-& alpha;) released from activated macrophages has various functions such as modulation of cell growth and differentiation, immunoreg-ulation, coagulation, and regulation of endothelial cell function. This study investigated the immunohistochemical staining of caspase-3 and TNF-& alpha; expression in the placentas of pregnant women with preterm premature rupture of membranes (PPROM). Methods: Placen-tas of 25 healthy, and 25 women with PPROM were processed for routine histological tissue processing. Placentas were stained with hematoxylin-eosin, caspase-3, and TNF-& alpha; immunostaining. Results: Normal placental histology was observed in the control group. Amniotic epithelium, vascular structures, and fibrinoid accumulation were histologically normal. Leukocyte infiltration, thinned vessel walls with dilatation and congestion, syncytial nodes, and fibrinoid accumulation were increased in the PPROM group. The immune activity of caspase-3 expression was mainly negative in placental components such as syncytial nodes, vascular endothelium, fibrinoid accumulation, and macrophages in the control group. In the PPROM group, caspase-3 positive reaction was increased in the amniotic membrane and epithelium, endothelial cells, fibrinoid accumulation, and areas of inflammatory cell infiltration. In the control group, negative TNF-& alpha; expression was observed in the placental membranes and structures. In the PPROM group, TNF-& alpha; expression was in-creased in inflammatory cells, endothelial cells, and syncytial nodes. Conclusions: Placentas of patients with PPROM showed loss and weakened membranes with increased placental pathology, and increased expression of caspase-3 and TNF-& alpha;. We suggest that caspase-3 and TNF-& alpha; signaling pathways can be used as a marker in the progression of PPROM.en_US
dc.identifier.doi10.31083/j.ceog5008173
dc.identifier.issn0390-6663
dc.identifier.issn2709-0094
dc.identifier.issue8en_US
dc.identifier.scopus2-s2.0-85171789843
dc.identifier.scopusqualityQ4
dc.identifier.urihttps://doi.org/10.31083/j.ceog5008173
dc.identifier.urihttps://hdl.handle.net/11468/19026
dc.identifier.volume50en_US
dc.identifier.wosWOS:001068567200010
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherImr Pressen_US
dc.relation.ispartofClinical and Experimental Obstetrics & Gynecology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCaspase-3en_US
dc.subjectTumor Necrosis Factor -Alphaen_US
dc.subjectPlacentaen_US
dc.subjectPremature Rupture Of Membranesen_US
dc.titleThe Investigation of Caspase-3 and Tumor Necrosis Factor-Alpha Expression in Placentas of Patients with Preterm Premature Rupture of Membranesen_US
dc.titleThe Investigation of Caspase-3 and Tumor Necrosis Factor-Alpha Expression in Placentas of Patients with Preterm Premature Rupture of Membranes
dc.typeArticleen_US

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