Diş Hekimliği Fakültesi Periodontoloji Anabilim Dalı Koleksiyonu
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Öğe Evaluating the effect of jointly administering synthetic graft and ankaferd blood stopper for bone recovery in created bone deformities in diabetic rats(Marmara Üniversitesi Sağlık Bilimleri Enstitüsü, 2023) Tanık, Abdulsamet; Doğru, Arzum Güler; Gül, MehmetObjective: In the present paper, it was purposed to examine the late impacts of bone tissue recoveries in combination with synthetic grafts and Ankaferd Blood Stopper (ABS), which are effective in bone wound healing in diabetic rats. Methods: A total of 64 Wistar albino male rats with diabetes were studied. A bone deformity was generated in the calvarium of diabetic rats. These diabetic rats are divided into 4 different groups. Only saline was applied to the bone defect in group 1, Beta-tricalcium phosphate (β-TCP) graft was administered to Group 2, ABS solution to Group 3, (β-TCP+ABS) were administered to group 4. Eight rats from each group were sacrificed on the 28th day and another 8 rats on the 56th day. Immunohistochemical, histopathological, and Dual Energy X-ray Absorptiometry (DEXA) analyzes of the obtained samples were made. Results: In histopathological measurements, osteoblastic activity and bone regeneration were considerably higher in the group treated with group 2 and group 4 when compared to the control group on the 28th and 56th day (p˂ .05). Western blotting findings showed that the osteopontin (OP) and osteonectin (ON) expression at 28th day increased dramatically in the treated with group 4. DEXA analyzes revealed that BMC values in groups treated with group 2, group 3 and group 4 on 28th day were considerably higher than the control group (p˂ .05). Conclusion: We can believe and conclude that ABS in combination with a β-TCP bone graft will produce more successful outcomes on wound healing and formation of new bone in diabetic rats.Öğe Evaluation of the effect of EDTA on root coverage at free gingival graft procedure(Uluslararası Diş Araştırmaları Birliği, 2018) İzol, Bozan Serhat; İpek, Fikret; Öner, Devrim Deniz; Arpağ, Osman FatihAim: The present study was designed to compare the effect of root surface biomodification with ethylenediaminetetraacetic acid (EDTA) for the treatment of buccal gingival recession using the free gingival graft (FGG) technique.Methodology: In this randomized controlled parallel clinical trial, 34 patients with 40 teeth with Miller Class I and Class II gingival recessions were treated using FGG. The 40 teeth with recessions were assigned randomly to receive FGG with or without the application of EDTA gel. On pre-treatment (Day 0) and at 3-months and 6-months post-treatment, the following parameters were measured: gingival recession height (GRH), gingival recession width (GRW), probing pocket depth (PD), clinical attachment level (CAL), and width of keratinized tissue (WKT).Results: In the FGG+EDTA group, statistically significant changes from baseline were found: GRH decreased from 4.71.5 mm to 1.31.2 and WKT increased from 0.90.9 mm to 5.51.8 mm. In the FGG group, GRH decreased from 4.61.3 mm to 1.31.2 mm and WKT increased from 0.90.7 mm to 5.41.5 mm. For the FGG and FGG+EDTA groups, averagerootcoveragewasfoundtobe74.14%and69.26%, respectively.Conclusions: Thedatademonstratedthattherootsurface biomodification agent, EDTA, had no beneficial effect on root coverage.Öğe Is there a relation between dental calculus and kidney stone?(Uluslararası Diş Araştırmaları Birliği, 2021) Yıldırım, Kadir; Yıldırım, Tuba Talo; Kaya, Filiz AcunAim: The purpose of this research is to investigate the frequency of dental calculus in patients with kidney stone and without kidney stone. Methodology: Two hundred eighty-seven patients (143 men and 144 women) aged between 18 and 68 (mean age 38.38±13.74) were included in the study. Patients were divided two groups in which 143 subjects without kidney stones (group I) and 144 subject with kidney stones (group II). Intragroup and inter-group analyzes were performed in terms of periodontal status and dental calculus index according to educational level, frequency of toothbrushing and smoking. Results: The study sample consisted of 287 patients which 143 in group I, 144 in group II. The mean age of the group I was 36.77±12.81 years old, and group II was 39.98±14.47 years old. There was a statistically significant difference between periodontal status and education level, daily tooth brushing and smoking in group I and group II (p<0.05). Both in group I and group II there was a statistically significant difference periodontal calculus index and education level, smoking, daily tooth brushing (p<0.05). In addition, there were significant correlations between dental calculus index and size of the kidney stone. Conclusion: There is a significant difference between the groups in terms of dental calculus index. There is a need for additional study on this subject in the future.Öğe Effects of nicotine administration in rats on MMP2 and VEGF levels in periodontal membrane(Via Medica, 2018) Deveci, Büşra; Ayna, Buket Erol; Tacir, İbrahim Halil; Tuncer, Cudi Mehmet; Pala, A.Background: Nicotine is associated with increased incidence of periodontal disease and poor response to therapy. This article aimed at identifying the expression of matrix metalloproteinases 2 (MMPs2) and vascular endothelial growth factor (VEGF) proteins on extracellular matrix, fibrous distribution and angiogenetic development in periodontitis caused by nicotine effects on periodontal membrane. Materials and methods: In this experimental study, rats were divided into nicotine and control groups. While the rats in the nicotine group (n = 6) were administered 2 mg/kg nicotine sulphate for 28 days, the animals in the control group (n = 6) were only administered 1.5 mL physiologic saline solution subcutaneously for 28 days. Results: Histological sections were prepared and immunohistochemically stained for MMP2 and VEGF. The sections stained with Trichrome-Masson were observed under light microscope. VEGF and MMP2 immunoreactivity of periodontal gingiva and dentin was assessed by immunohistochemical staining. Conclusions: Nicotine reduces MMP production, disrupts collagen synthesis and causes periodontitis. We observed that nicotine increases periodontitis by disrupting periodontal membrane and prevents tooth to anchor in dental alveoli by disrupting epithelial structure.Öğe Effect of silver diamine fluoride application on fluoride concentration in saliva(Institute of Physics Publishing, 2018) Widianti, T. A.; Bahar, Armasastra; Maharani, Diah Ayu; Tümen, Emin Caner; Yavuz, İzzetSilver diaminefluoride can increase fluoride concentration in saliva, facilitate remineralization, and increase the bioavailability of fluoride in saliva. The purpose of this study was to analyze the difference in fluoride concentration in saliva before and after silver diaminefluoride application on enamel. Stimulated saliva of four subjects was collected, and the concentration of fluoride was measured. The results showed that there were significant differences in the fluoride concentration (p<0.05) before, immediately after, and one hour after silver diaminefluoride application on enamel. It can be concluded that fluoride concentration reached its peak immediately after silver diamine fluoride application on enamel and that it had returned to the baseline one hour after application.Öğe Comparison of the effect of periodontal therapy and diode decontamination and periodontal therapy alone on the amount of TNF-alpha in systemically healthy chronic periodontitis patients a non-randomized clinical trial(SCI Printers & Publication INC, 2020) Doğru, Arzum Güler; Toprak, Ömer; Üner, Devrim Deniz; İzol, Bozan Serhat; Kaplan, İbrahim; Doğru, Mehmet; İpek, FikretAbstract OBJECTIVE: To evaluate the outcome of initial periodontal therapy and additional diode laser decontamination therapy on clinical parameters and the amount of tumor necrosis factor alpha (TNF-alpha) in gingival crevicular fluid (GCF) in patients with chronic periodontitis. STUDY DESIGN: The study included 22 patients who were diagnosed with chronic periodontitis and who had at least 1 (>= 5 mm) periodontal pocket in the posterior region of each upper left hemi-arcade. The study was designed as a split-mouth study involving mechanical therapy on the right side and nonsurgical periodontal therapy plus diode laser decontamination therapy on the left side. RESULTS: A statistically significant decrease was observed in clinical parameters at 3 months when compared to the values at baseline in both treatment areas. The change in clinical parameters at 3 months after therapy was not significantly different between the groups (p>0.05). The comparison of GCF TNF-alpha levels at baseline and at 3 months in the 2 treatment areas identified significant changes in the TNF-alpha levels in the hemi-arcade treated with laser diode contamination therapy, whereas the changes in the hemi-arcade treated with nonsurgical periodontal therapy were not significant (p>0.05). A between-group comparison revealed no statistically significant difference at 3 months post-therapy, while the difference at 1 month was found to be statistically significant. CONCLUSION: The study found that laser therapy in addition to nonsurgical periodontal therapy has a positive effect on the clinical parameters and proinflammatory TNF-alpha levels in the early period but makes no contribution in the long term.Öğe Ganoderma lucidum, a promising agent possessing antioxidant and anti-inflammatory effects for treating calvarial defects with graft application in rats(Sociedade Brasileira para o Desenvolvimento de Pesquisa em Cirurgia, 2019) Laçin, Nihat; İzol, Serhat Bozan; İpek, Fikret; Tuncer, Cudi MehmetPurpose: Ganoderma lucidum, a kind of mushroom used for its antioxidant, anti-inflammatory, and immunomodulatory activities, was investigated in the present study for its possible healing effect on calvarial defects with bone grafts. Methods: Wistar male rats (n = 30) were divided into 3 groups: 1) the control (defect) group (n = 10), 2) defect and graft group (n = 10), and 3) defect, graft, and G. lucidum treated group (n = 10). The G. lucidum was administered to the rats at 20 mL/kg per day via gastric lavage. Results: In the defect and graft group, osteonectin positive expression was observed in osteoblast and osteocyte cells at the periphery of the small bone trabeculae within the graft area. In the defect, graft, and G. lucidum treated group, osteonectin expression was positive in the osteoblast and osteocyte cells and positive osteonectin expression in new bone trabeculae. The expression of matrix metalloproteinase-9 (MMP-9) was positive in the inflammatory cells, fibroblast cells, and degenerated collagen fibril areas within the defect area. Conclusion: This study shows that, with its antioxidant and anti-inflammatory properties, G. Lucidum is an important factor in the treatment of calvarial bone defects.Öğe An evaluation of the effects of caffeic acid phenethyl ester and ankaferd blood stopper on secondary wound healing of oral mucosal tissue(Turkiye Klinikleri, 2020) Gül, Mehmet; Günay, Ahmet; Tanık, AbdülsametBackground/aim: Caffeic acid phenethyl ester (CAPE) and Ankaferd Blood Stopper (ABS) are considered to contribute to wound healing. The purpose of this study was to investigate the effect of ABS and CAPE on secondary wound healing of oral mucosal tissue. Materials and methods: In total, 63 male Sprague-Dawley rats were used in this study. The animals were randomly divided into three groups and anaesthetized with ketamine (8 mg/100 g, intraperitoneally): a control group, CAPE group, and ABS group. A full-thickness excisional wound was created using a 4 mm punch biopsy tool. Topical ABS and CAPE were then applied in each group for 7, 14, and 21 days (n = 7 in each group). The animals in each group were sacrificed after 7, 14, and 21 days. Palatal specimens were stained with haematoxylin-eosin. Vascular endothelial growth factor (VEGF) and tumour necrosis factor-inducible gene 6 (TSG-6) protein expressions were determined using the Western blot method. Results: Inflammation, vessel dilatation, and haemorrhages were significantly lower in the CAPE group as compared with these parameters in the other groups (P < 0.05). Fibrosis was significantly higher in the ABS group as compared with that in the other groups (P < 0.05). VEGF protein levels were elevated in the 21-day CAPE group and 7-day ABS group. The expression of TSG-6 increased in the 7-day CAPE group and 21-day ABS group. Conclusion: Based on our findings, ABS and CAPE had positive effects on the oral wound healing process.Öğe Evaluation of the effects of bone morphogenetic protein-2 on the healing of bone calvarial defects in ovariectomized rats(TUBITAK, 2020) Kadiroğlu, Ela Tules; Karayürek, Fatih; Akbalık, Mehmet ErdemBone morphogenetic proteins (BMPs) are often used with bone grafts for bone regeneration. The aim of this study was to evaluate the effect of demineralized freeze-dried bone allografts and externally applied rhBMP-2 on bone healing in experimentally generated defects in the calvarium of ovariectomized and nonovariectomized rats via histological and immunohistochemical assays. The current study was carried out on 42 female Wistar rats. After ovariectomy was performed in half of the rats, the rats were divided into three groups: Control, allograft, and allograft + BMP. An experimental defect with a diameter of 4 mm was created unilaterally in the parietal bone using a trephine dental drill and a physiodispenser with saline. All animals were euthanized at the end of week 8, and bone tissues were analyzed histologically and immunohistochemically. The allografts produced better results in terms of calvarial defect healing in both the ovariectomized and nonovariectomized groups compared to the control group in enhancing bone healing, whereas BMPs combined with allografts had no positive effect on new bone formation. The allografts and BMPs applied in both groups produced fibrous tissues rather than new bone tissue. Further comprehensive studies on the effectiveness of BMPs in the formation of new bone tissue should be conducted in animals with estrogen deficiency.Öğe Calvarial bone defects in ovariectomised rats treated with mesenchymal stem cells and demineralised freeze-dried bone allografts(Via Medica, 2020) Kadiroğlu, Ela Tules; Akbalık, Mehmet Erdem; Karaöz, Erdal; Kanay, Berna Ersöz; Dağ, Ahmet; Ketani, Muzaffer Aydın; Eroğlu, E. G.; Uysal, Ersin; Tuncer, Cudi M.Background: The aim of the study was to investigate the ability of a combination of bone marrow mesenchymal stem cells (BM-MSCs) with and without demineralised freeze-dried bone allografts (DFDBAs) to induce bone regeneration in calvarial defects in ovariectomised rats. Materials and methods: Critical size defects were filled with a combination of DFDBAs and BM-MSCs or BM-MSCs alone. Eight weeks after calvarial surgery, the rats were sacrificed. The samples were analysed histologically and immunohistochemically. Results: No difference was observed in vascularisation between groups C1 (animals with cranial defect only, control group) and O1 (animals with cranial defect only, ovariectomy group). Intramembranous ossification was observed at a limited level in groups C2 (animals with cranial defect with MSCs, control group) and O2 (animals with cranial defect with MSCs, ovariectomy group) compared to C1 and O1. In group C3 (animals with DFDBAs with MSCs, control group), the fibrous structures of the matrix became compact as a result of a bone graft having been placed in the cavity, but in group O3 (animals with DFDBAs with MSCs, ovariectomy group), the fibrous tissue was poorly distributed between the bone grafts for the most parts. Conclusions: We conclude that the insertion of BM-MSCs enhances bone healing; however, the DFDBA/BM-MSC combination has little effect on overcoming impaired bone formation in ovariectomised rats.Öğe To what extent does hyaluronic acid affect healing of xenografts? A histomorphometric study in a rabbit model(SciElo, 2018) Arpağ, Osman Fatih; Damlar, İbrahim; Altan, Ahmet; Tatlı, Ufuk; Günay, AhmetAmong the many graft materials that have been used for the treatment of bone defects in oral and maxillofacial regions is xenograft. To improve osteoconductive effects of xenografts, they have been combined with various biocompatible materials, such as hyaluronic acid and bone morphogenetic protein. Objective: To determine bone-healing capacity of high molecular weight hyaluronic acid (HA) combined with xenograft in rabbit calvarial bone defects. Material and methods: Ten adult male New Zealand rabbits (mean weight 3 kg) were included in the study. Three 6-mm-diameter bicortical cranial defects were created on calvarial bone of all rabbits. These defects were filled as follows: a) xenograft; b) HA+xenograft; c) autograft. One month after the first operation, rabbits were sacrificed. Specimens were evaluated histomorphometrically. Results: Considering multiple comparisons, differences regarding new bone were statistically significant between all groups (p<0.05). The volume of residual graft was significantly decreased in HA group compared to xenograft group (p=0.035). Marrow space, trabecular thickness (TbTh), trabecular width (TbWi), trabecular separation (TbSp), and number of node: number of terminus (NNd:NTm) in the autograft group were significantly better than xenograft and HA groups (p<0.05). However, regarding marrow space, TbTh, TbWi, TbSp, and NNd:NTm values, xenograft and HA groups showed similar results and the difference were not significant (p>0.05). Conclusion: These results support that high molecular weight hyaluronic acid could contribute to the healing of xenograft by improving the percentage of new bone formation and reducing the percentage of residual graft. However, HA did not significantly affect the quality of newly formed bone assessed by microarchitectural parameters.Öğe Streptozotocin ile oluşturulmuş deneysel diyabetin, ratların dilleri üzerine etkisinin histolojik olarak araştırılması(Dicle Üniversitesi Veteriner Fakültesi, 2016) Dağ, Ahmet; Uysal, Ersin; Ketani, Muzaffer Aydın; Kadiroğlu, Ela Tules; Kanay, Berna ErsözBir çok çalışmada,diyabetus mellituslu hastalarda tat bozuklukları ve dilde lezyonlar meydana geldiği ileri sürülmektedir. Ama bu konuda histolojik çalışmalar azdır. Bu çalışmanın amacı, streptozotocin ile oluşturulan deneysel diyabetin ratların dilleri üzerinde ne tür değişiklikler oluşturabileceğini histolojik olarak ışık mikroskopu ile incelemektir. Bu çalışmada 250-300 g ağırlığında 15 erkek Wistar rat kullanıldı. Hayvanlar deneyden önce rastgele iki gruba ayrıldı. Grup-1 deneysel diyabet, 10 ratta streptozotosin enjeksiyonu ile oluşturuldu. Grup-2 kontrol grubu oluşturdu (5 rat). Diyabetin streptozotosin ile indüklenmesinden sonraki 7. ve 14. günlerde, her gruptaki beş hayvan sacrifiye edildi. Histolojik analiz için dilleri kullanıldı. Dilin histolojik özelliklerinin değerlendirilmesinde, diyabet grubunun epitel kalınlığında artış görüldü. Ayrıca diyabet grubunda kontrol grubundan daha yüksek seviyede inflamatuar hücre infiltrasyonu olduğu tespit edildi ve bu bulgu istatistiksel olarak anlamlıydı.Sonuç: Çalışmamızın bulgularına göre, diyabet dilin morfolojik yapısını değiştirerek inflamasyona daha yatkın hale getirebileceği söylenebilir.
