Streptozotocin ile oluşturulmuş deneysel diyabetin, ratların dilleri üzerine etkisinin histolojik olarak araştırılması
Yükleniyor...
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Dicle Üniversitesi Veteriner Fakültesi
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Bir çok çalışmada,diyabetus mellituslu hastalarda tat bozuklukları ve dilde lezyonlar meydana geldiği ileri sürülmektedir. Ama bu konuda histolojik çalışmalar azdır. Bu çalışmanın amacı, streptozotocin ile oluşturulan deneysel diyabetin ratların dilleri üzerinde ne tür değişiklikler oluşturabileceğini histolojik olarak ışık mikroskopu ile incelemektir. Bu çalışmada 250-300 g ağırlığında 15 erkek Wistar rat kullanıldı. Hayvanlar deneyden önce rastgele iki gruba ayrıldı. Grup-1 deneysel diyabet, 10 ratta streptozotosin enjeksiyonu ile oluşturuldu. Grup-2 kontrol grubu oluşturdu (5 rat). Diyabetin streptozotosin ile indüklenmesinden sonraki 7. ve 14. günlerde, her gruptaki beş hayvan sacrifiye edildi. Histolojik analiz için dilleri kullanıldı. Dilin histolojik özelliklerinin değerlendirilmesinde, diyabet grubunun epitel kalınlığında artış görüldü. Ayrıca diyabet grubunda kontrol grubundan daha yüksek seviyede inflamatuar hücre infiltrasyonu olduğu tespit edildi ve bu bulgu istatistiksel olarak anlamlıydı.Sonuç: Çalışmamızın bulgularına göre, diyabet dilin morfolojik yapısını değiştirerek inflamasyona daha yatkın hale getirebileceği söylenebilir.
Many studies suggest that taste disorders and tongue lesions associate with diabetes mellitus (DM). However, the tongue histopathology underlying impairments in taste sensation, tongue lesions in the DM is poorly examined. The present study aimed to clarify histological changes of the tongues in streptozotocin (STZ)-induced diabetic and control rats by using light microscopy. Fifteen male Wistar rats that weighed 250–300 g were used in this study. The animals were randomly divided into two groups prior to the experiment. Group 1 irreversible experimental diabetes was created by streptozotocin injection in 10 rats. Group 2 comprised the control group(5 rats). On the 7th and 14th days after the induction of diabetes by streptozotocin, five animals from each group were euthanized by cardiac puncture under intraperitoneal anesthesia with ketamine HCI and xylazine. Tongue’s were performed for the histological analysis. Changes in the histological characteristics of the lingual; An increase in the total thickness of the epithelium of the diabetes group was observed. Furthermore, it was found that inflammatory cell infiltration was higher in the diabetic group than in the control group, and this excess was statistically significant. Conclusion: According to the findings of our study, it can be said that diabetic can change the morphological structure of the tongue to make inflammation more susceptible.
Many studies suggest that taste disorders and tongue lesions associate with diabetes mellitus (DM). However, the tongue histopathology underlying impairments in taste sensation, tongue lesions in the DM is poorly examined. The present study aimed to clarify histological changes of the tongues in streptozotocin (STZ)-induced diabetic and control rats by using light microscopy. Fifteen male Wistar rats that weighed 250–300 g were used in this study. The animals were randomly divided into two groups prior to the experiment. Group 1 irreversible experimental diabetes was created by streptozotocin injection in 10 rats. Group 2 comprised the control group(5 rats). On the 7th and 14th days after the induction of diabetes by streptozotocin, five animals from each group were euthanized by cardiac puncture under intraperitoneal anesthesia with ketamine HCI and xylazine. Tongue’s were performed for the histological analysis. Changes in the histological characteristics of the lingual; An increase in the total thickness of the epithelium of the diabetes group was observed. Furthermore, it was found that inflammatory cell infiltration was higher in the diabetic group than in the control group, and this excess was statistically significant. Conclusion: According to the findings of our study, it can be said that diabetic can change the morphological structure of the tongue to make inflammation more susceptible.
Açıklama
Anahtar Kelimeler
Dil, Streptozotosin, Diabetes mellitus, Rat, Tongue, Streptozotocin
Kaynak
Dicle Üniversitesi Veteriner Fakültesi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
2
Sayı
2
Künye
Dağ, A., Uysal, E., Ketani, M. A., Kadiroğlu, E. T. ve Kanay, B. E. (2016). Streptozotocin ile oluşturulmuş deneysel diyabetin, ratların dilleri üzerine etkisinin histolojik olarak araştırılması. Dicle Üniversitesi Vetriner Fakültesi Dergisi, 2(2), 112-117.
