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Öğe Complexation and mutagenicity potential studies with N,N?-bis(2-hydroxynaphthalin-1-carbaldehydene)-1,2-bis-(P-aminophenoxy)ethane and a novel oxovanadium(IV) complex(Taylor & Francis Ltd, 2004) Temel, H; Çakir, Ü; Tolan, V; Otludil, B; Ugras, HIA new oxovanadium(IV) complex of the Schiff base obtained by condensation of 1,2-bis(p-aminophenoxy)ethane with 2-hydroxynaphthalin-1-carbaldehyde was synthesized. The complex was characterized by elemental analysis, magnetic measurements, mass spectral data, UV-visible and IR spectra. Stability constants and thermodynamic values for complexation between Cu(NO3)(2), Zn(NO3)(2) (.) 6H(2)O, Ni(NO3)(2) (.) 6H(2)O and VOSO4 (.) 5H(2)O and the ligand in 80% dioxane/water were determined by conductance measurements. Ni(II) and Cu(II) complexes of the ligand synthesized by a previously described method were found to be mutagens on strain TA 100 in the presence and absence of S9 mix. These compounds are classified as mutagenic in the Ames test.Öğe Effect of bovine serum albumine on production of alpha-amylase and amylase thermostability in Bacillus subtilis(Mbr Press Inc, 1997) Otludil, B; Ensari, NY; Aguloglu, S; Uyar, FThermal stability of alpha-amylase from Bacillus subtilis isolated from thermal Cermik hot spring was studied. alpha-amylase was purified from media with and without bovine serum albumen (BSA). The enzyme was stable at 68 degrees C when purified from media with different concentrations of BSA;however,it was observed to be stable at 75 degrees C when interacted with different concentrations of BSA. B. subtilis was grown in different concentrations of BSA. The best bacterial growth was identified to be in the medium with 180 mu g/ml BSA. Production of alpha-amylase and proteases was induced in presence of 180-240 mu g/ml BSA.Öğe The effect of phenolic amino acids on differentiation of DNA, RNA, proteins and cell growth in Cicer arietinum L.(Mbr Press Inc, 1999) Otludil, B; Akbayin, H; Taskin, T; Demir, RThe effect of three amino acids (phenilalanine tyrosine triptophane) have been examined on differentiation of DNA, RNA, protein synthesis and cell growth in cell culture for Cicer arietinum L. cultivated in Murashige and Skoog medium. The Cicer arietinum L, culture were treated with 20, 40,60,80 and 100 mu g. levels of each phenolic amino acid. Amount of DNA, RNA, proteins and cell growth were determined by the levels in the time intervals of 24 h incubation time during 6 days. Obtained data were evaluated statistically Consequently, experimental results reveal that excessive amount of phenolic amino acids cause significant inhibition on RNA, protein and cell growth. Phenilalanine not strongly effect on DNA synthesis or activity of DNA polymerase was unaffected by phenilalanine but tyrosine and triptophane has significant effect on DNA. On the other hand, it has been supposed that extreme amount of phenolic amino acids may be converted to phenolic compounds by cell regulatory systems.Öğe The effects of endosulfan on the great ramshorn snail Planorbarius corneus (Gastropoda, Pulmonata)(Pergamon-Elsevier Science Ltd, 2004) Otludil, B; Cengiz, EI; Yildirim, MZ; Ünver, Ö; Ünlü, EIn this study the great ramshorn snail (Planorbarius corneus), one of the most abundant gastropod of Turkish limnic systems, was investigated to determine the histopathological effects of endosulfan on the digestive gland, foot and mantle under laboratory conditions. Samples were collected from small artificial pools in Karaot at Gelendost-Isparta (Southwest of Turkey), where agricultural activities are widespread. The snails were exposed to two sublethal concentrations of endosulfan (0.4 and 0.8 mg/l) for periods of 10, 20 and 30 days. Fifteen snails were kept in 2.5 1 glass jars containing dechlorinated tap water and exposed under semi-static test (daily exchange of test water). All the testing was carried out on adult specimens, and snails were maintained on a photothermal period with 16 light hours at 22+/-2 degreesC. The histopathological examinations revealed the following changes: amoebocytes infiltration, dilatation in hemo-lymphatic spaces between the tubules, degeneration of cells, abnormal lumen, necrosis of cells and atrophy in the connective tissue of digestive gland; desquamation of the epithelium cells, changes in the number of mucocytes and protein gland cells, lipid vacuolus and atrophy of the columnar muscle fibers of the foot and mantle tissues. Pycnotic state of cells was also seen in the mantle tissues. Endosulfan caused significant histopathological alterations in the digestive gland, foot and mantle tissues of the snail, irrespective of concentrations of the pesticide and its exposure periods. The results are discussed, particularly in comparison to those of other aquatic organisms. (C) 2004 Elsevier Ltd. All rights reserved.Öğe The formation of callus cultures from unpollinated pistils in cotton plant (Gossypium hirsutum L)(Mbr Press Inc, 1998) Taskin, T; Demir, R; Otludil, B; Colak, GIn this study, unpollinated floral buds of cotton (Gossypium hirsutum L.) were cultured on the Murashige and Skoog (MS) media containing Kinetin (Kin) and Indol Asetic Acid (IAA) at different concentrations for the callus cultures, The nutrient medium containing 2 mg/l Kin + 1 mg/l IAA was observed to be the best medium for the callus formation from ovule and ovarium tissues.Öğe Intravesical oxybutynin application: Ultrastructural effects on bladder epithelium.(Mbr Press Inc, 1999) Ersay, A; Ketani, MA; Nergiz, Y; Demirtas, OC; Akkus, M; Otludil, BIntravesically applied oxybutynin, rapidly absorbs into the bloodstream, additional to profound local effect. Currently morphologic effects of oxybutynin on local bladder tissue relatively well established at light microscopic level, but not ultrastructural level. Thirty New Zealand White female rabbits were catheterized daily and intravesical instillation were performed with whether 1mg/kg oxybutynin solution or saline for 30 days. The local effects of the drug on bladder epithelium at electron microscopic level were examined comparing with saline administration. Urinary tract infection (UTI) incidence were similar in both saline and oxybutynin groups (9 vs. 10 of 15 animals respectively) (p>0.05). Interestingly, in 4 of 5 animals that received oxybutynin and never had UTI during the study, separation of zonula occludens intercellular junction was determinated by Jeol electron microscope. This observation can explain rapid absorption of oxybutynin through the bladder into the blood stream. Oxybutynin can damage bladder surface epithelium at ultrastructural level. This effect may lead to increase absorption of it, but not result in higher incidence of bacterial infection.Öğe Investigation of the saturated and unsaturated fatty acids in the internodes and leaves of Vitis vinifera L. (Mazruma)(Mbr Press Inc, 1997) Demir, R; Otludil, BIn this study, the amounts of saturated (palmitic acid, stearic acid) and unsaturated (oleic acid, linoleic acid, linolenic acid) fatty acids in the samples belonging to internodes 1-2-3, 5-6 and 9-10, and of saturated (palmitic acid, stearic acid) and unsaturated (oleic acid, linoleic acid, linolenic acid) fatty acids in the leaf samples belonging to internodes 1-2-3, 5-6 and 9-10 of Vitis vinifera L.(Mazruma) were investigated, depending on the vegetation period. According to ripening period, great variations were seen in the total fatty acids amounts. Unsaturated fatty acids were found to be higher with respect to saturated fatty acids. However, any significant differences were not found between saturated and unsaturated fatty acids in the specimens belonging to all internodes and in the leaf samples belonging to these internodes.Öğe Purification and characterization of alkaline proteinase from alkalophilic Bacillus sp.(Maik Nauka/Interperiodica/Springer, 2001) Muderriszade, A; Ensari, NY; Aguloglu, S; Otludil, BAlkaline proteinase was purified from Bacillus sp. isolated from soil. The pH optimum was 11.5 at 37 degreesC. Calcium divalent cation was effective in stabilizing the enzyme, especially at higher temperatures. The proteolytic activity was inhibited by the specific serine proteinase inhibitor PMSF (phenylmethylsulfonyl fluoride), and ions of Mg, Mn, Pb, Li, Zn, Ag, and Hg. The enzyme was stable in the presence of detergents, such as Triton-X100, Tween-80. SDS (sodium dodecyl sulfate), and EDTA (ethylenediaminetetraacetic acid), at pH 11.5 and 37 degreesC for 30 min. The optimum pH was 11.5 at 37 degreesC, and the optimum temperature was 62 degreesC at pH 11.5.Öğe Synthesis, spectral and biological studies of Mn(II), Ni(II), Cu(II), and Zn(II) complexes with a tetradentate Schiff base ligand.: Complexation studies and the determination of stability constants (Ke)(Taylor & Francis Inc, 2001) Temel, H; Çakir, Ü; Otludil, B; Ugras, HIThe complexes Mn(II), Ni(II), Cu(II) and Zn(H) ions with a N2O2 Schiff base derived from 1,4-diaminobutane and salicylaldehyde, N,N'-bis(salicylidene)-1,4-diaminobutane (LH2), have been prepared and characterized by elemental analyses, molar conductivities, spectral (IR, NMR, visible, UV) and magnetic moment measurements. Stability constants were measured by means of a conductometric method. Furthermore, the stability constants for complexation between ZnCl2, Cu(NO3)(2) and AgNO3 salts and the ligand in 80% dioxane-water and pure methanol were determined by conductance measurements. The magnitudes of these ion association constants are related to the nature of the solvation of the cation and the complexed cation. The mobilities of the complexes are also dependent, in part, upon solvation effects. The complexes Mn(II), Ni(II), Cu(II) and Zn(II) ions with the Schiff base have been evaluated for their antibacterial activity against rec(-)and rec(+) stains of Bacillus subtilis.
