Yazar "Gokalp, Osman" seçeneğine göre listele
Listeleniyor 1 - 12 / 12
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Factors affecting mortality in patients with organophosphate poisoning(Pakistan Medical Assoc, 2015) Gunduz, Ercan; Dursun, Recep; Icer, Mustafa; Zengin, Yilmaz; Gullu, Mehmet Nezir; Durgun, Hasan Mansur; Gokalp, OsmanObjective: To investigate the relationship between clinical and demographic characteristics and mortality in patients with organophosphate poisoning. Methods: The retrospective study was conducted at Dicle University, Diyarbakir, Turkey, and comprised data of patients who presented with organophosphate poisoning between April 2004 and April 2013. The records were assessed in two groups, with Group 1 having data related to recovery, and Group 2 having data related to mortality. SPSS 16 was used for statistical analysis. Results: Of the 296 patients, 219(74%) were women. Mortality was the outcome in 41(13.9%) cases. In Group 2, mean age, marital status, rural origin, presence of psychiatric disease, being illiterate, presence of nicotinic symptoms, and late admission were significantly higher than Group 1 (p<0.05 each). Logistic regression analysis indicated bradycardia as the most prominent independent predictor of mortality (p<0.001). Other independent predictors were age, glucose level, lactate dehydrogenase, coma and acidosis (p<0.05 each). Conclusion: Independent predictors of mortality in patients with organophosphate poisoning as bradycardia, age, glucose, lactate dehydrogenase level and acidosis.Öğe HISTOPATHOLOGIC EFFECTS OF GLASS IONOMER BONE CEMENTS APPLICATION TO MAXILLOFACIAL AREA: AN EXPERIMENTAL STUDY IN RABBITS(Diagnosis Press Ltd, 2012) Yorgancilar, Ediz; Firat, Ugur; Gun, Ramazan; Bakir, Salih; Dasdag, Suleyman; Akkus, Zeki; Gokalp, OsmanReconstruction of the maxillofacial bone defects and fractures poses a challenge to the surgeons. Various alternatives and materials have been described for these defects and fractures. Glass ionomer bone cements (GICs) have been used extensively in dentistry but recently they have also been utilized in otolaryngology. We hypothesized that GIC can be an alternative material for maxillofacial reconstruction. However, their biocompatibility is of primary importance because this material will be in direct contact with the tissue for a prolonged tune and might affect it. Therefore the aim of this study was to investigate the tissue responses to GIG in the maxillofacial area in rabbits. The study was carried out on 16 New Zealand White rabbits, which were divided into study (n: 8) and control (n: 8) groups. Experimental defects and fractures were created in the nasal bone, maxilla and zygoma in both the study and the control group. The experimental fractures and defects were reconstructed by GIG in the study group. However, the rabbits in the control group were left to natural healing process. The inflammatory reaction and fibrosis in the rabbits of both the study and the control group were compared by using descriptive histopathological analysis 180 days after application. The tissue reactions were graded. GIG showed a slight inflammatory and fibrous reaction in the rabbit of the study group. Nevertheless, statistical difference between the groups was not observed in terms of inflammatory reaction and fibrosis (P>0.05). The results of this study indicated that GIG is a well tolerated material in maxillofacial reconstruction.Öğe Iloprost relaxes phenylephrine-precontracted rat aortic rings(E-Century Publishing Corp, 2016) Peker, Recep Oktay; Donmez, Soner; Cankara, Fatma Nihan; Dogan, Emre; Gokalp, OsmanIloprost is a prostacyclin analogue mainly used in the treatment of pulmonary hypertension. It is also effective for renal dysfunction during renal transplant and coronary artery bypass grafting surgery, but there have been few comparative experimental studies with iloprost. We aimed to compare vasorelaxant properties of iloprost, with those of diltiazem, nitroglycerin and papaverine on rat thoracic aorta following phenylephrine-induced precontraction. Thirty-two young adult female Wistar albino rats were used in the study. After isolation of thoracic aorta distal to arcus, 3 mm aortic rings were placed in an organ bath and phenylephrine (10(-6) M) was added for precontraction. Subsequently, cumulative concentrations of iloprost, nitroglycerin, diltiazem and papaverine were added to aortic rings on separate chambers and dose-response curves were recorded. The mean (+/- SD) maximal relaxation (E-max) was 27.1 +/- 2.9% for iloprost, 111.7 +/- 2.1% for nitroglycerin, 77.4 +/- 2.9% for diltiazem and 147.2 +/- 2.8% for papaverine. The half maximal effective concentration (EC50) values were 2.4 x 10(-11) molar (M) for iloprost, 5.6 x 10(-8) M for nitroglycerin, 7.0 x 10(-7) M for papaverine and 2.1 x 10(-5) M for diltiazem. There was a statistically significant difference in Emax values and EC50 values between four groups (P<0.001 and P<0.01, respectively). Although a vasorelaxant response was observed with iloprost, it was less compared to papaverine, nitroglycerin and diltiazem on the isolated rat aortic rings. Iloprost has also the lowest EC50 value which could be valuable in clinical practice.Öğe Impact of caffeic acid phenethyl ester treatment on vancomycin-induced pancreatic damage in rats(Sage Publications Inc, 2016) Koyu, Ahmet; Gokalp, Osman; Gumral, Nurhan; Oktem, Faruk; Karahan, Nermin; Yilmaz, Nigar; Saygin, MustafaThis study investigates the preventive effect of caffeic acid phenethyl ester (CAPE) on pancreatic damage induced by vancomycin (VCM) in rats. Rats were equally divided into three groups: group I (control), group II (only VCM-treated group) and group III (VCM + CAPE-treated groups). VCM was intraperitoneally administered at a dose of 200 mg kg(-1) twice daily for 7 days. CAPE was administered orally at 10 mu M mL(-1) kg(-1) dose once daily for 7 days. The first dose of CAPE administration was performed 24 h prior to VCM injection. Blood and pancreas tissue samples were removed and collected after the study. Serum alkaline phosphatase (ALP), amylase, g-glutamyl transferase (GGT) and lipase activities were determined. Pancreas tissue samples were evaluated with the light microscope. Group II significantly increased serum ALP, amylase, GGT and lipase activities when compared with the control group. Group III significantly decreased serum ALP, amylase, GGT and lipase activities when compared with group II. In histopathological examination, it has been observed that there was a significant pancreatic damage in group II. CAPE exerted prominent structural protection against VCM-induced pancreatic damage and this effect was statistically significant. CAPE caused a marked reduction in the extent of pancreatic damage. We have concluded that it may play an important role in the VCM-induced pancreatic damage and reduce the pancreatic damage both at the biochemical and histopathological aspects.Öğe Increased Caspase-3 Immunoreactivity of Erythrocytes in STZ Diabetic Rats(Hindawi Publishing Corporation, 2012) Firat, Ugur; Kaya, Savas; Cim, Abdullah; Buyukbayram, Huseyin; Gokalp, Osman; Dal, Mehmet Sinan; Tamer, Mehmet NumanEryptosis is a term to define apoptosis of erythrocytes. Oxidative stress and hyperglycemia, both of which exist in the diabetic intravascular environment, can trigger eryptosis of erythrocytes. In this experimental study, it is presented that the majority of erythrocytes shows caspase-3 immunoreactivity in streptozocin- (STZ)-induced diabetic rats. Besides that, caspase-3 positive erythrocytes are aggregated and attached to vascular endothelium. In conclusion, these results may start a debate that eryptosis could have a role in the diabetic complications.Öğe Justification for the Use of CA 125 Levels After Cardiac Surgery(Akad Doktorlar Yayinevi, 2009) Peker, R. Oktay; Peker, Tuelay T.; Varol, Ercan; Ozaydin, Mehmet; Gulmen, Senol; Gokalp, Osman; Kaya, SavasCA 125, a tumor marker has been found to be elevated in malign conditions as well as in some benign conditions like heart failure. Cardiac surgery has been shown to cause a systemic inflammatory response. In this study, we investigated alterations in serum levels of CA 125 during cardiac surgery with cardiopulmonary by-pass. Thirty nine patients with a mean age of 54.7 years who underwent either coronary bypass surgery or valvular heart surgery were prospectively recruited to the study. We measured plasma levels of CA 125 preoperatively and on postoperative days I and 7. Comparing with preoperative values, CA 125 levels were similar on postoperative day 1(5.26 [6.89] U/ml vs 5.74 [4.54] U/ml) but elevated significantly on postoperative day 7 (42.1 [34] U/ml, p< 0.0001) (data in median interquantile range). CA 125 levels were found to be elevated after cardiac surgery. The elevations are more in patients undergoing valvular heart surgery than coronary bypass graft surgery. Although CA 125 can be considered a reliable tumor marker in the diagnosis and follow up of patients with malignant diseases, the presence of a recent cardiac surgery with cardiopulmonary bypass must be taken into account when asking for the cause of elevated CA 125 plasma level.Öğe Magnesium and diltiazem relaxes phenylephrine-precontracted rat aortic rings(Oxford Univ Press, 2012) Dogan, Mustafa; Peker, Recep O.; Donmez, Soner; Gokalp, OsmanPerioperative vasospasm during cardiovascular surgery is a challenging problem. Several vasodilator agents are frequently utilized for its prevention in surgical practice. Magnesium and diltiazem both have known potential vasorelaxant effects. We planned to compare the efficacy of diltiazem and magnesium in relieving phenylephrine-precontracted rat aortic rings. Ten young adult female Wistar albino rats weighing 230-260 g were used in this study. The aortic rings in the organ bath equilibrated and reached their baseline tension. Precontraction was induced by 0.001 mmol/l phenylephrine and cumulative concentration-relaxation curves were obtained by consecutively increasing the addition of either diltiazem (10(-6)-0.1 mmol/l) or magnesium (0.1-10 mmol/l). The mean maximal relaxation responses observed by diltiazem and magnesium on separate aortic rings were 90 +/- 3 and 53 +/- 2%, respectively. The calculated EC50 of diltiazem was 0.01035 mmol/l, whereas the EC50 of magnesium was 4.064 mmol/l (P < 0.05). Both magnesium and diltiazem produced vasorelaxation on phenylephrine-precontracted rat aortic rings in this study, but the potency of diltiazem regarding the EC50 value was significantly higher than that of magnesium. Magnesium could be a candidate together with diltiazem to inhibit vasospasm on arterial grafts during coronary bypass surgery.Öğe Mild hypoglycaemic attacks induced by sulphonylureas related to CYP2C9, CYP2C19 and CYP2C8 polymorphisms in routine clinical setting(Springer Heidelberg, 2011) Gokalp, Osman; Gunes, Arzu; Cam, Hakan; Cure, Erkan; Aydin, Osman; Tamer, Mehmet Numan; Scordo, Maria GabriellaTo evaluate the impact of polymorphisms in the cytochrome P450 (CYP) 2C9, 2C19 and 2C8 genes on the risk of mild hypoglycaemic attacks in patients treated with sulphonylureas. One hundred and eight type 2 diabetic patients (50 men, 58 women), treated with oral antidiabetics, including at least one from the sulphonylurea group (glimepiride n = 50, gliclazide n = 46, or glipizide n = 12) for 3 months or longer, were included in the study. Symptoms of hypoglycaemia (sweating, tremor, anxiety and palpitations) during a 3 month period were recorded and confirmed by home glucose measurements. Gender, age, body mass index, creatinine clearance, HbA1c, oral antidiabetic dose and concomitant medication were assessed together with functional CYP2C9, CYP2C19 and CYP2C8 polymorphisms, analysed by real-time PCR methods. Fifteen patients (eight men, seven women) reported hypoglycaemia symptoms which were validated by their home glucose measurements (< 70 mg/dl). Heterozygosity and homozygosity for CYP2C9 variant alleles (*2 or *3) tended to be more frequent among patients who reported hypoglycaemic attacks (60 and 7%) than those who did not (39 and 3%). Similarly, the CYP2C8*1/*3 genotype tended to be more frequent in patients with (47%) than without (27%) hypoglycaemia, while no such trend was observed for CYP2C19 variants. However, only in the gliclazide group a significant association between CYP2C9 genotype and hypoglycaemic attacks was observed (P = 0.035). None of the other covariates showed any significant association with the risk of hypoglycaemic attacks. CYP2C9 polymorphisms leading to decreased enzyme activity show a modest impact on the risk of mild hypoglycaemia attacks during oral antidiabetic treatment, with a significant association in patients treated with gliclazide.Öğe Phosalone Toxicity on Liver and Pancreas: Role of Vitamins E and C(Asian Journal Of Chemistry, 2013) Demirin, Hilmi; Gokalp, Osman; Kaya, Ertugrul; Buyukvanli, Bora; Cesur, Gokhan; Ozkan, Aybars; Kaya, MuratPhosalone (6-chloro-3-[diethoxyphosphinothioylsulfanylmethyl]-1,3-benzoxazol-2-one) is one of the most commonly used organophosphorus pesticides in the peat control of crops. Subchronic phosalone exposure was evaluated for its effects on the serum activities of some enzymes concerning hepatic and pancreatic damage including aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutamyltransferase (GGT), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), cholinesterase (ChE); and finally protective effects of combination of vitamins E and C in 24 wistar-albino rats. Experimental groups were as follows: control group (n = 8); a group treated with 120 mg/kg body weight phosalone (P group, n = 8); and a group treated with 120 mg/kg body weight phosalone + vitamin E + vitamin C (P+V group, n = 8). The P and P+V groups were treated orally with phosalone on 5 days a week for 4 weeks. The serum activities of the above mentioned enzymes were analyzed. In the samples phosalone significantly increased the activities of ALT, LDH and decreased ChE (p < 0.05). However no significant change was detected for the remainder enzymes (p > 0.05). In the P+V group, ALT and LDH activities were significantly increased and ChE decreased (p < 0.05). It is concluded that subchronic phosalone causes rat liver damage to an extent, which is somewhat reflected on the liver enzymes. Furthermore, a combination of vitamins E and C can reduce the toxic effects of phosalone on liver tissue of rats.Öğe Protective role of caffeic acid phenethyl ester on serum cholinesterase inhibition by acute exposure to diazinon in rats(Tubitak Scientific & Technological Research Council Turkey, 2014) Oguzhanoglu, Esin; Andac, Ahmet Cenk; Tufek, Adnan; Yavuz, Lutfi; Vural, Huseyin; Gokalp, OsmanAim: To evaluate whether caffeic acid phenethyl ester (CAPE), a flavonoid-like natural compound plentifully found in beeswax, has a protective effect on diazinon-induced serum cholinesterase (ChE) inhibition in rats. Materials and methods: Animals were divided into 4 groups. The first animal group was not treated with any substance. The second animal group was orally given a 200 mg/kg body weight (bw) sublethal dose of diazinon. The third animal group was injected intraperitoneally with 2.84 mg (10 mu mol)/kg bw of CAPE 1 day prior to administration of 200 mg/kg bw of diazinon orally. The fourth animal group was intraperitoneally injected with 2.84 mg (10 mu mol)/kg bw of CAPE 30 min after 200 mg/kg bw of diazinon was orally administered. Results: Analysis of the animal blood samples obtained 48 h after diazinon administration revealed that diazinon decreased serum ChE activity by 75%, while CAPE administration 24 h prior to and 30 min following diazinon application improved serum ChE activity by 25%-32% as compared to levels with diazinon administration only. In silico studies suggest that CAPE prevents diazinon from binding to butyryl ChE due to a higher binding affinity than that of diazinon. Conclusion: Our laboratory findings suggest that CAPE plays a protective role against butyryl ChE inhibition by diazinon.Öğe Relationships Between Respiratory Function Disorders and Serum Copper Levels in Copper Mineworkers(Humana Press Inc, 2012) Abakay, Abdurrahman; Gokalp, Osman; Abakay, Ozlem; Evliyaoglu, Osman; Sezgi, Cengizhan; Palanci, Yilmaz; Ekici, FaysalThe aim of this study was to investigate the respiratory function disorders that could be related to dust exposure during the production of copper mine in copper mineworkers (CMWs). The study included 75 male CMWs (mean age, 32.0 +/- 7.1 years, 58.6% smokers) and 75 male age- and smoking status-matched healthy control subjects. Serum Cu level was significantly higher in the CMW group (0.80 +/- 0.62 mu g/ml) than the control group (0.60 +/- 0.39 mu g/ml) (p = 0.017). Significant negative correlations were found between serum Cu level and forced expiratory volume in first second (r = -0.600; p < 0.001) and between serum Cu level and forced vital capacity (r = -0.593; p = < 0.001) in CMWs. Serum Cu level was significantly higher in the restrictive type pulmonary function disorders group (1.36 +/- 0.62 mu g/ml) than obstructive type (0.90 +/- 0.55 mu g/ml) and normal pulmonary function pattern group (0.53 +/- 0.43 mu g/ml) (p < 0.001). Patients with radiological parenchymal abnormalities had significantly higher serum copper levels than those without abnormalities (1.53 +/- 0.52 vs. 0.71 +/- 0.52 mu g/ml, respectively; p = 0.002). In conclusion, result of the study has shown a negative association between pulmonary functions disorders and radiological abnormalities and serum Cu levels in CMWs.Öğe Vasorelaxant effects of dobutamine and levosimendan on rat aorta rings(Baycinar Medical Publ-Baycinar Tibbi Yayincilik, 2015) Dogan, Emre; Peker, Recep Oktay; Yener, Ali Umit; Donmez, Soner; Gokalp, OsmanBackground: In this experimental study, the vasorelaxant effects of levosimendan and dobutamine on isolated rat thoracic aorta preparations were compared. Methods: Sixteen Wistar albino type male rats were used. The thoracic aortas were removed carefully and were transferred to petri dishes containing Krebs solution. Aortic rings of approximately 5 mm in length were prepared and placed in the organ bath. Contraction and relaxation forces of the aortic rings were recorded. Contraction response was obtained by applying 10(-5)M phenylephrine to aortic rings. Subsequently, cumulative doses of levosimendan (10(-8)M-10(-4)M) was applied to eight aortic rings and cumulative doses of dobutamine (10(-8)M-10(-3)M) was applied to the other eight aortic rings and dose-response curves were recorded. The EC50 and pD2 values were calculated by the computer program named Graph Pad Prisim 4.0. The Mann-Whitney U-test was performed for statistical analysis. Results: The relaxation response of the aortic rings with levosimendan 10(-4)M administration was %92.33 while the relaxation response with dobutamine 10-4M administration was %82.48. It did not show a significant difference between both relaxation responses (p=0.059). The EC50 value was calculated as 6.605x10(-6)M for dobutamine and 5.093x10(-5)M for levosimendan. The pD2 value was found to be 5.2 +/- 0.4 for levosimendan and 4.3 +/- 0.2 for dobutamine. Conclusion: Levosimendan and dobutamine molecules in vitro rat aortic rings have similar vasorelaxant effects.
