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Öğe Adenosine deaminase activity in diagnosis of tuberculous ascites(1997) Özden, Meltem; Canoruç, Fikri; Erdinç, Levent; Kaplan, Abdurrahman; Mekik, Haluk; Işık, Fatma BirgülBetween April 1993-September 1995, we studied the activity of adenosine deaminase (ADA) in the peritoneal fluid of 72 patients who were divided into three groups according to causes of ascites, as follows: tuberculous peritonitis (group I), secondary to malignant tumors (group II), cirrhosis of the liver (group III). In tuberculous ascitic fluid, ADA levels were 55.4±7.3 IU/L (mean±SD), which was significantly more than that noted in cirrhotic (7.6±3.9 IU/L), or malignant (15.5±7.7 IU/L) ascitic fluids (P<0.001, for each). At a cutt-off value of greater than 30IU/L, the sensitivity, specificity, positive and negative predictive values were 95.7%, 100%, 92.5% and 100%, respectively. These findings suggest that the ascitic fluid ADA activity may be used to identify patients in whom the diagnosis of abdominal tuberculosis must be pursued.Öğe The effect of fish oil on liver and plasma MDA and antioxidant status of rats(1999) Mete, Nuriye; Işık, Fatma Birgül; Gürkan, Fuat; Erdinç, LeventThe question how fish oil (FO) and soybean oil (SO) change the state of antioxidant and the lipid peroxidation in liver and plasma of rats and the protective role of vitamin E were investigated. Six-week-old forty male Sprague-Dawley rats were divided into five groups. One of the groups fed a control diet, another one fed SO, while the other three groups fed FO diet at the level of 10% Vitamin E supplementation was maintained to three different levels 3, 50, 150 IU/per kg diet in the FO diets and 3 IU vitamin E per kg of the SO diet. At the end of tenth week, blood was drawn under ether anesthesia and finally plasma and red blood cell samples were separated. Liver tissues were washed by 0.9% NaCl, then dried by a filter paper and stored at -60oC. The malondialdehyde (MDA) amount as an indicator of lipid peroxidation was not significantly affected in the SO group, while it was found increased both in serum and liver tissue in the FO group with vitamin E supplementation of 3 IU/kg diet (P<0.001). Erythrocyte GSH levels and liver GSH-Px activity were not significantly different between the SO and the control group, while there were significant differences in the FO group with the lowest dose of vitamin E (p<0.001). Catalase activity was not statistically different between groups. MDA production decreased and the antioxidant status was improved by higher doses of vitamin E fed together with FO. A statistically significant positive correlation was found between dietary vitamin E and plasma vitamin C, erythrocyte GSH and liver GSH-Px (P<0.01). In conclusion decreased antioxidant defence and increased MDA amount in the FO fed rats changed to normal with vitamin E supplementation. As significant correlations were found between vitamin E and other antioxidants, vitamin E seems necessary to maintain appropriate levels of other physiological antioxidants.Öğe The effects of melatonin, fluoxetine and their combinations on stress induced behavioral and cognitive impairments in mice(Ankara Üniversitesi Eczacılık Fakültesi, 2023) Çamçi, Merve İnci; Erdinç, Meral; Kelle, İlker; Uyar, Emre; Erdinç, LeventObjective: Melatonin (Mel) is a hormone with anti-depressant and anti-oxidant features. It is well known that melatonin protects brain cells from reactive oxygen species and that the brain's high oxygen consumption and lipid content make it particularly vulnerable to oxidative stress caused by prolonged stress. This study aims to investigate the effects of melatonin, fluoxetine and their combinations on emotional memory, depression, and anxiety-like behavioral changes induced by immobilization (Imb) stress. Material and Method: 48 male Balb/c mice were divided into eight groups: Cnt (control), Imb, Imb+Mlt, Imb+Flx (fluoxetine), Imb+Mlt+Flx, Mlt, Flx and Mlt+Flx. For seven days in a row, the mice underwent daily immobilization stress for 6 hours. Mice were treated with Mlt (10 mg/kg) and Flx (20 mg/kg). All animals were subjected to the behavioral tests; forced swimming test (FST), open field test (OFT), elevated plus maze (EPM), passive avoidance test (PAT) and hot plate (HP) test. After all behavioral tests, brain tissues were obtained for malondialdehyde level analysis. Result and Discussion: OFT test data showed the time spent in the central zone and the number of entrances to the central area were significantly lower in the Imb group compared to the Cnt group, these were higher in the Imb+Flx, Imb+Mlt, Imb+Mlt+Flx groups compared to the Imb group. Also, according to the data obtained from FST, immobile time was significantly higher in the Imb group compared to the Cnt group, it was lower in the Imb+Flx, Imb+Mlt, Imb+Mlt+Flx groups compared to the Imb group. Besides, it was demonstrated that the emotional memory index was statistically higher in the Imb group compared to the Cnt group, and the increasing of memory index returned to normal in the Imb+Mlt and Imb+Mlt+Flx groups with PAT. And also, lipid peroxidation level, which increased in the Imb group, decreased significantly in the Imb+Flx, Imb+Mlt, and Imb+Mlt+Flx groups. As a result, it was observed that melatonin has anti-depressant, anxiolytic, antioxidant effects and normalized emotional memory. Also, melatonin, fluoxetine and their combinations exert similar effects in the present study.Öğe The Effects of nifedipine on renal perfusion pressure and kidney during cisplatin-induced nephrotoxicity in rats(Dicle Üniversitesi Tıp Fakültesi, 2007) Erdinç, Meral; Erdinç, Levent; Nergiz, Yusuf; Kelle, İlkerCisplatin is one of the most effective cancer chemotherapeutic agent used against various solid tumors. Nephrotoxicity is one of the major dose-limiting side effects of cisplatin. It has been known that different mechanisms as oxidative stress may play an important role in cisplatin induced nephrotoxicity resulted with changes in renal haemodynamics. This study was performed to investigate the effect of nifedipine –one of the dihydropyridine calcium antagonist on changes in renal perfusion pressures and kidneys of rats with cisplatin nephrotoxicity. Male wistar albino rats were divided into 3 groups (n=8):1-Control group(1 ml saline. i.p) 2-Cisplatin group (a single dose of cisplatin (5 mg/kg, i.p) 3- A single dose of cisplatin (5 mg/kg, i.p) + Nifedipine (2 mg/kg/day, i.p) for five days. When those pre-treated groups compared with control group, perfusion pressures, serum urea and creatinine levels and tissue MDA levels were found significantly higher in cisplatin group (p<0.001). Histopathological examination showed widespread tubular necrosis and dilatation in cisplatin-treated group versus other groups. In cisplatin + nifedipine pretreated group, perfusion pressures, serum urea and creatinine levels and tissue MDA levels found significantly lower than cisplatin group (p<0. 001) and less tubular dilatation and necrosis was observed. As a result it was demonstrated that Nifedipine has protective effects against cisplatin nephrotoxicity. We suggest that this is partly provided by the beneficial effects of nifedipine on altered renal haemodynamics during cisplatin nephrotoxicity.Öğe Evaluation of serum interleukin-38 levels in different radiographic grades of idiopathic knee osteoarthritis(Mary Ann Liebert, INC, 2021) Duman, Baver Akcan; Duman, Serda; Çamurcu, Yalkın; Gem, Mehmet; Erdinç, LeventThe aim of this study was to assess interleukin-1 beta (IL-1 beta), IL-1Ra, IL-36, and IL-38 levels together with hs-CRP levels in patients with different radiographic grades of knee osteoarthritis (OA) in comparison to healthy individuals. Consecutive patients aged over 50 years who were admitted to our Orthopaedics and Traumatology department between November 2018 and March 2019 and diagnosed as knee OA according to the American College of Rheumatology criteria were included in this prospective case-control study. Patients with knee OA were staged according to radiographic Kellgren-Lawrence (K-L) classification and 20 patients were assigned to each group. An age and gender matched control group consisted healthy volunteers with no clinical and radiographic sign of arthritis were conducted as the control group. Venous blood samples were collected and assessed for hs-CRP, IL-1 beta, IL-1Ra, IL-36, and IL-38 levels using the double-antibody sandwich ELISA method. The hs-CRP, IL-1 beta, IL-36 and IL-38 levels did not significantly differ among controls and independent radiographic stage groups except IL-1Ra levels which was significantly higher in K-L grade 4 knee OA groups compared to healthy controls (P = 0.045). When we compared all patients with knee OA and healthy controls, we detected that IL-1 and IL-1Ra were significantly lower and IL-38 levels were significantly higher in healthy control group compared to patients with knee OA (P = < 0.001, < 0.001, and 0.019, respectively). According to results obtained from our study, IL-1 beta, IL-1Ra, and IL-38 levels significantly differed between healthy individuals and patients with knee OA. However, we did not observe a significant difference and correlation between radiographic grade of knee OA and interleukin levels.Öğe Karaciğer sirozunda plazma karnitin düzeyleri(1995) Erdinç, Levent; Canoruç, Fikri; Canoruç, Naime; Batun, M. Sabri; Kaplan, Abdurrahman; Turgut, Cengiz[Abstract Not Available]Öğe Oksitosin ile uyarılan sıçan uterus şeritlerinde L-arjinin gevşeme yanıtlarının gebelik dönemleri süresince değişimi.(2000) Erdinç, Meral; Yıldızhan, Pekin Begüm; Çiçek, Ramazan; Erdinç, Levent; Güneli, EnsariAMAÇ: L-arjinin - nitrik oksit sisteminin siklik guanozin monofosfat aracılığı ile ,uterus düz kası dahil olmak üzere birçok düz kasta gevşemeye yolaçtığı bilinmektedir. Uterusda L-arjinin-nitrik oksit sisteminin gebelik süresince Jzole sıçan ve insan uterus şeritlerinin spontan kontraksiyonları üzerine değişen şekilde inhibe edici etkisi olduğu bildirilmiştir. Bu çalışmada gebeliğin farklı dönemlerinde oksitosin ile uyarılan sıçan uterus şeritlerindeki kontraksiyonlara L-arjinin 'in etkisi araştırıldı. GEREÇ VE YÖNTEM: Gebe olmayan ve gebeliğin orta ve geç dönemlerinde izole edilen sıçan uterus şeritlerinde organ banyosunda 0,5 mU/mi konsantrasyonunda oksitosin ile uyarılan kontraksiyonlar ve buna L-arjinin'in 1.5, 3 mM konsantrasyonlarındaki etkisi izotonik kasılma olarak kimografa kaydedilerek değerlendirildi.İstatistiksel hesaplamalarda p<0,05 olarak kabul edilerek, Kruskall Wallis ve Mann Whitney U -testleri ile analiz edildi. BULGULAR: Gebe olmayan sıçanlardan izole edilen uterus şeritlerinde 0.5 mU/ml oksitosin ile uyarılan kasılmalar 1.5-3 mM L-arjinin ile anlamlı olarak azaldı (p< 0,05). Gebeliğin orta döneminde gerek oksitosin gerekse L-arjinin yanıtları gebe olmayan gruba göre anlamlı olarak değişmezken, gebeliğin son döneminde oksitosin yanıtları, gebeliğin orta dönemi ile karşılaştırıldığında anlamlı olarak arttığı görüldü (p< 0,05). Buna karşılık yine gebeliğin son döneminde L-arjinin gevşeme yanıtlarının gebeliğin orta dönemi ve gebeliğin olmadığı grupla karşılaştırıldığında anlamlı olarak azaldığı görüldü (p< 0,05). SONUÇ: L-arjininin-nitrik oksit sisteminin uterus düz kasında gevşetici etkisinin gebelik dönemlerinde değişiklik gösterdiği .gebeliğin son döneminde azaldığı tesbit edilerek, bu değişim sürecinin uterusun gebelik süresince sessiz kalmasında ve doğumun başlamasında kontraktil aktivitenin baskın hale geçmesinde rolü olduğu kanısına varıldı.Öğe Protective effects of necrostatin-1 on doxorubicin-induced cardiotoxicity in rat heart(Sage Publications INC., 2022) Özgen, Zeynep Erdoğmuş; Erdinç, Meral; Kelle, İlker; Erdinç, Levent; Nergiz, YusufBackground: Doxorubicin (Dox) is one of the most effective antineoplastic drugs which has severe cardiotoxic effects, limiting its clinical usage. Though the exact mechanism of doxorubicin-induced cardiotoxicity is yet to be elucidated, it is shown that production of reactive oxygen species (ROS) increases oxidative stress and leads to cardiomyocyte apoptosis and necroptosis which is also defined as a programmed cell death. Purpose: In the present study, we investigate the effects of necrostatin-1 (Nec-1)-an inhibitor of receptor interaction proteins 1 (RIP1) and necroptosis-on doxorubicin-induced cardiotoxicity in rats. Research Design: Hearts were isolated and perfused by the Langendorff system in all four groups. Perfusion pressure (PP), left ventricular developed pressure (LVDP) and heart rate per minute (HR), LV (dP/dt) max, and LV (dP/dt) min which shows cardiac contractility and relaxation were recorded. Results: Results showed that PP significantly increased with Dox treatment and significantly decreased with Nec-1 treatment, while HR, LVDP, LV (dP/dt) max, and LV (dP/dt) min values significantly decreased with the Dox-treated group and significantly increased with Nec-1 treatment. Also with Nec-1 treatment, gene expression levels of anti-apoptotic Bcl-2 significantly increased and pro-apoptotic protein Bax, apoptotic marker caspase-3, and Nox-2 significantly decreased compared to the Dox-treated group. In heart tissues, MDA levels were significantly increased with Dox and decreased with Nec-1 treatment. These results were supported by the histological analysis indicated that Nec-1 reduced doxorubicin-induced cellular injury. Conclusions: In conclusion, our data indicate that Nec-1 ameliorates doxorubicin-induced cardiotoxicity by reducing oxidative stress injury and attenuating apoptosis and necroptosis.Öğe Sıçan uterus şeritlerinde nitrik oksit sentaz inhibisyonunun oksitosin kontraksiyonlarına etkisi(2000) Erdinç, Levent; Erdinç, Meral; Çiçek, Ramazan; Yıldızhan, Pekin Begüm; Güneli, EnsariAMAÇ: L-arjinin aminoasidinden nitrik oksit sentaz enzimi ile sentezlenen nitrik oksitin siklik guanozin monofosfat aracılığı ile birçok düz kasta ve uterusta gevşeme yaptığı bilinmektedir. Bu çalışmada izole uterus şeritlerinde nitrik oksit sentaz inhibisyonunun oksitosin ile uyarılan kontraksiyonlar üzerine etkisi araştırıldı. GEREÇ VE YÖNTEM: Dişi Sprague-Dawley sıçanlardan izole edilen uterus şeritleri, organ banyosundaki ortamda 3 mM konsantrasyonda L-Name varlığında ve yokluğunda, 0.5 mU/ml oksitosin ile uyarılarak, kontraksiyon yanıtları izonik olarak kimografa kaydedildi. Gruplar arasındaki fark istatistiksel olarak Mann Whitney U testi ile değerlendirilerek, p< 0.05 anlamlı olarak kabul edildi. BULGULAR: Oksitosin ile oluşturulan kontraksiyonların hem frekansının hem de amplitüdünün L-Name varlığında anlamlı şekilde arttığı gözlendi (p< 0.05). SONUÇ: L-arjinin-Nitrik oksit sisteminin inhibisyonunun uterus kasılmalarında anlamlı artışa yolaçtığımn gözlenmesi, bu sistemin uterusun kontraktil fonksiyonları üzerinde önemli etkiye sahip olduğu sonucunu ortaya çıkarmaktadır.Öğe Sıçanlarda etanol ve stresle oluşturulan gastrik mukozal tahribatın E-vitamini ile değişimi(1995) Kaplan, Abdurrahman; Yazanel, Orhan; Canoruç, Fikri; Erdinç, Levent; Batun, SabriÇeşitli doku hasarından sorumlu olan serbest ok¬sijen radikalleri ve bu radikallerin elimine edilmesinde kullanılan antioksidan maddelerden E vitamininin et¬kileri bu deneysel çalışmada incelenmiştir. Toplam 34 sıçdn 4 gruba ayrılarak (kontrol grubu, stres grubu, stres+Etanol grubu ve stres+Etanol+E vitamini grubu) mide dokularında serbest oksijen radikallerinin bir me-taboliti olan Malondialdehit (MDA) düzeyleri "thiobar-bitürik asit metodu" ile ölçüldü. MDA düzeyleri stres ve stres+etanol gruplarında kontrol grubuna göre yüksek bulundu(p<0.01). E vitamini verilen stres+Etanol gru¬bunda ise, MDA düzeyleri kontrol düzeylerine yakın bulundu (p>0.05). Sonuç olarak E vitamini lipid perok-sidasyonunu azalttığı, dolayısı ile mokozal tahribata engel olduğu görüldü.Öğe The value of cerebrospinal fluid adenosine deaminase determination in the diagnosis of tuberculous meningitis(1997) Erdinç, Levent; Işık, Fatma Birgül; Batun, Sabri; Bircan, Zelal; Mete, MahmutTüberküloz meninjitli beş, bakteriyel meninjitli 14, viral meninjitli altı ve febril konvülziyonlu dokuz hastada beyin omurilik sıvısı (BOS)'nda adenozin deaminaz (ADA) enzim aktivitesi ölçüldü. Tüberküloz meninjitli hastaların BOS ADA aktiviteleri öteki gruplara göre anlamlı derecede yüksek bulundu (p < 0.01). 11.0 U/litre'lik bir cut-off değeri kullanıldığında testin tüberküloz meninjitin tanısında duyarlılığı % 100, özgüllüğü ise % 96 olarak saptandı.
