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Öğe Assessment of antimicrobial and antioxidant activities of nepeta trachonitica: Analysis of its phenolic compounds using HPLC-MS/MS(MDPI, 2017) Köksal, Ekrem; Tohma, Hatice; Kılıç, Ömer; Alan, Yusuf; Aras, Abdülmelik; Gülçin, İlhami; Bursal, ErcanContinuing our work on the sources of natural bioactive compounds, we evaluated the antimicrobial and antioxidant activities of Nepeta trachonitica as well as its major phenolic content using the high-performance liquid chromatography-mass spectrometry/mass spectrometry (HPLC-MS/MS) technique. For antioxidant activity, ferric reducing antioxidant power (FRAP) and cupric ion reducing antioxidant capacity (CUPRAC) methods were performed to measure the reducing power and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was employed to evaluate the radical scavenging activity of the sample. For antimicrobial activity, three Gram-positive and four Gram-negative microbial species as well as three fungi species were tested. N. trachonitica appeared to have reasonable antioxidant activity and decent antimicrobial activity as indicated by the inhibition of the organisms’ growth. The most susceptible species were Bacillus subtilis ATCC 6633 and Escherichia coli ATCC 11229 among the organisms tested. Ethanol extract of the plant has the highest effect on Saccharomyces cerevisiae but no effect on Yarrowia lipolytica. The HPLC-MS/MS analysis showed that at least 11 major phenolic compounds of N. trachonitica exist, the major ones being rosmarinic acid, chlorogenic acid and quinic acid. The obtained results suggest that N. trachonitica could be a promising source for food and nutraceutical industries because of its antimicrobial and antioxidant properties and phenolic compounds.Öğe Chemical Content by LC-MS/MS, Antiglaucoma, and Antioxidant Activity of Propolis Samples From Different Regions of Türkiye(Wiley, 2024) Izol, Ebubekir; Bursal, Ercan; Yapici, Ismail; Yilmaz, Mustafa Abdullah; Yilmaz, Isa; Gulcin, IlhamiPropolis is a sticky substance produced by bees because of the reaction of beeswax, pollen, and bee enzymes. Particularly, their biological activity and chemical content attract attention. Thus, in this study, the total amount of phenolic and flavonoid substances, Fe3+-Fe2+, Cu2+ (cupric ions reducing activity [CUPRAC]), and Fe3+-TPTZ (ferric ions reducing antioxidant power [FRAP]) reducing, and DPPH center dot and ABTS center dot+ scavenging assays in vitro antioxidant properties of propolis samples obtained from four different provinces of T & uuml;rkiye were determined. In addition, the chemical content of propolis samples was quantitatively determined by LC-MS/MS, and the antiglaucoma property was revealed by hCAII enzyme inhibition. Propolis samples from Ordu presented the highest amounts of total phenolic and flavonoid content (492.3 +/- 5.8 and 96.1 +/- 2.1, respectively) and also highest antioxidant activity (DPPH center dot and ABTS center dot+ IC50 [mu g/mL]: 8.884 +/- 0.84 and 4.589 +/- 0.80, respectively; Fe+3, CUPRAC, and FRAP: 1.051 +/- 0.012, 1.021 +/- 0.008, and 0.957 +/- 0.007 mu g/mL, respectively). hCAII enzyme inhibition was highest in Mu & scedil; propolis (IC50 [mu g/mL]: 8.6) as determined. By LC-MS/MS, 53 different components were screened and 35 bioactive components were determined. According to the results, propolis was found to be a raw material because it contains high concentrations of acacetin, chrysin, caffeic acid, and quinic acid (123.824, 24.759, 47.779, and 16.32 mg analyte/g extract, respectively).Öğe Determination of antioxidant potential of Nepeta nuda subsp. lydiae(Taylor and Francis Ltd., 2016) Aras, Abdulmelik; Doğru, Mehmet; Bursal, ErcanThe antioxidant activities of ethanol and water extracts of Nepeta nuda subsp. lydiae leaves were determined by different in vitro methods such as; DPPH, ABTS, FRAP and CUPRAC assays. The results were compared to BHA, BHT and ascorbic acid as standard antioxidant compounds. Both water and ethanol extracts presented high antioxidant potential on DPPH free radical scavenging assay and CUPRAC assay. Also, both extracts presented low level on ABTS and FRAP assays. In conclusion, leaves of N. nuda subsp. lydiae have some potential of antioxidant activities, so it could be a useful source for food industry as a food ingredient after analyzing other biological properties.Öğe Enzyme inhibitory function and phytochemical profile of Inula discoidea using in vitro and in silico methods(Elsevier, 2021) Bursal, Ercan; Yılmaz, Mustafa Abdullah; İzol, Ebubekir; Türkan, Fikret; Atalar, Mehmet Nuri; Murahari, Manikanta; Aras, Abdulmelik; Ahmad, MushtaqMany plant species have a large diversity of secondary metabolites with different biological activities. This study aims to assess the phenolic constituent, enzyme inhibitory and antioxidant activities of the aqueous (water) and methanol extracts of Inula discoidea. The enzyme assays showed effective enzyme inhibition of the methanol extract against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), glutathione S-transferase (GST), and alpha-glycosidase (alpha-Gly) enzymes. The IC50 values for AChE, BChE, GST, and alpha-Gly were found as 38.5 mg/mL, 34.65 mg/mL, 77.0 mg/mL, and 40.76 mg/mL, respectively. Antioxidant properties of the aqueous and methanol extracts of I. discoidea were determined by four well-known in vitro techniques (ABTS, CUPRAC, DPPH, and FRAP methods). The antioxidant values of both water and methanol extracts were found to be better than the standard antioxidants (BHA, BHT, ascorbic acid, and alpha-tocopherol) in ABTS and CUPRAC methods. According to an updated LC-MS/MS technique analysis, quinic acid (21.08 mg/g), protocatechuic acid (4.49 mg/g), and gallic acid (0.48 mg/g) were found as major phenolic compounds of the plant extract. The binding interactions of major phenolic compounds of I. discoidea with the AChE, BChE, GST, and alpha-Gly enzymes were investigated by the molecular docking studies.Öğe Identification of Bioactive Polyphenolic Compounds and Assessment of Antioxidant Activity of Origanum acutidens(Scipress Ltd, 2017) Aras, Abdulmelik; Silinsin, Muzaffer; Bingol, Muhammed Nuri; Bursal, ErcanThe main objective of the current work is to search the antioxidant activities of Origanum acutidens leaves by using DPPH, FRAP and CUPRAC methods severally. Three well known antioxidant compounds (BHA, BHT and ascorbic acid) were used as standards for comparing the extracts. Also, phenolic compounds of Origanum acutidens leaves were identified by UHPLC-ESIMS/MS. The high concentrations of rosmarinic acid (11158.99 ppb) quinic acid (3200.84 ppb) and naringenin (1238.45 ppb) were detected quantitatively.Öğe Phenolic content, antioxidant potentials of Saponaria prostrata endemic plant(International Society of Academicians, 2022) Bursal, Ercan; Aras, Abdülmelik; Doğru, Mehmet; Kılıç, ÖmerSaponaria prostrata (S. prostrata) is an endemic and medicinal plant that contains secondary metabolites such as flavonoids, phenolic compounds, fatty acids, and triterpenoids. This study was carried out to evaluate the antioxidant potentials, and phenolic composition of S. prostrata. Antioxidant properties of the ethanol and water extracts of S. prostrata were evaluated by three different in vitro bioanalytical methods including CUPRAC and FRAP reducing antioxidant methods and DPPH radical scavenging antioxidant method. Effective antioxidant potentials of the plant extracts were found especially in the CUPRAC method. Rutin (36.3 µg/g extract) and hesperidin (32.7 µg/g extract) were characterized as major phenolic compounds of S. prostrata using an advanced HPLC technique.Öğe Polyphenolic Content, Antioxidant Potential and Antimicrobial Activity of Satureja boissieri(Jihad Daneshgahi, 2018) Aras, Abdulmelik; Bursal, Ercan; Alan, Yusuf; Turkan, Fikret; Alkan, Huseyin; Kilic, OmerAntioxidant activities of Satureja boissieri extracts were detected by using specific in vitro techniques. Standard antioxidant compounds such as ascorbic acid, BHA (Butylated Hydroxyanisole) and BHT (Butylated Hydroxytoluene) were used to compare with the results obtained from ethanol and water extracts of the plant samples. Both extracts presented high antioxidant actions on ABTS cation radical and DPPH free radical scavenging methods. Furthermore, the antimicrobial activity of S. boissieri was defined by using three fungi species, three Gram-positive and four Gram-negative bacteria species. The reducing power antioxidant activities of samples were measured by CUPRAC and FRAP techniques. Also, phenolic compounds of S. boissieri leaves were identified by UHPLC-ESI-MS/MS. The high concentration of hesperidin (5051 +/- 247 ppb) and rosmarinic acid (4364 +/- 214 ppb) was characterized quantitatively. According to the results, high phenolic content can be thought of as one of the responsible parameters for effective biological activity. Also, its flavonoid and phenolic contents are good natural sources for using in the food industry and pharmacological process.Öğe RP-HPLC/MS/MS Analysis of the Phenolic Compounds, Antioxidant and Antimicrobial Activities of Salvia L. Species(Mdpi, 2016) Tohma, Hatice; Koksal, Ekrem; Kilic, Omer; Alan, Yusuf; Yilmaz, Mustafa Abdullah; Gulcin, Ilhami; Bursal, ErcanThe identification and quantification of the phenolic contents of methanolic extracts of three Salvia L. species namely S. brachyantha (Bordz.) Pobed, S. aethiopis L., and S. microstegia Boiss. and Bal. were evaluated using reverse phase high performance liquid chromatography, UV adsorption, and mass spectrometry (RP-HPLC/MS). In order to determine the antioxidant capacity of these species, cupric ions (Cu2+) reducing assay (CUPRAC) and ferric ions (Fe3+) reducing assay (FRAP) were performed to screen the reducing capacity and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was employed for evaluation of the radical scavenging activity for both solvents. In further investigation, the antimicrobial activities of Salvia species were tested using the disc diffusion method against three Gram-positive and four Gram-negative microbial species, as well as three fungi species. The results showed that there is a total of 18 detectable phenols, the most abundant of which was kaempferol in S. microstegia and rosmarinic acids in S. brachyantha and S aethiopis. The other major phenols were found to be apigenin, luteolin, p-coumaric acid, and chlorogenic acid. All species tested showed moderate and lower antioxidant activity than standard antioxidants such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), and ascorbic acid. The ethanolic extracts of Salvia species revealed a wide range of antimicrobial activity. S. brachyantha and S. microstegia showed the highest antimicrobial activities against B. subtilis, whereas S. aethiopis was more effective on Y. lipolytica. None of the extracts showed anti-fungal activity against S. cerevisiae. Thus these species could be valuable due to their bioactive compounds.Öğe Unravelling the phenolic compound reserves, antioxidant and enzyme inhibitory activities of an endemic plant species, Achillea pseudoaleppica(Taylor & Francis, 2021) Yılmaz, Mustafa Abdullah; Taslimi, Parham; Kılıç, Ömer; Gülçin, İlhami; Dey, Abhijit; Bursal, ErcanThe present ethnobotanical study unravelled the phenolic reservoir (UHPLC-MS/TQ-MS) and pharmacological activity (antioxidant and enzyme inhibitory activities) of an endemic plant, Achillea pseudoaleppica Hub.-Mor. (Asteraceae). The effective antioxidant properties of ethanol and water extracts of A. pseudoaleppica leaves were determined by using six different in vitro bioanalytical methods including three reducing antioxidant methods and three radical scavenging antioxidant methods. In the other step of the study, the enzyme inhibitory effects of water and ethanol extracts of A. pseudoaleppica were determined against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), alpha-amylase, and alpha-glucosidase enzymes. The ethanol extract was found to have effective inhibition potential for all four respected enzymes. The IC50 values of A. pseudoaleppica extract against AChE, BChE, alpha-amylase, and alpha-glucosidase enzymes were found to be 2.67 mg/mL, 4.55 mg/mL, 16.51 mg/mL, and 12.37 mg/mL, respectively. Also, UHPLC-MS/TQ-MS analyses revealed quinic acid as the most abundant phenolic compound of the water extract (31.12 +/- 1.65 mu g/mg) and ethanol extract (11.75 +/- 0.82 mu g/mg). In addition, the molecular docking interaction of the most abundant phenolic compound of A. pseudoaleppica (quinic acid) with AChE, BChE, alpha-amylase, and alpha-glucosidase target enzymes were evaluated using Chimera and AutoDock Vina softwares. In conclusion, the rich phenolic content and the potent antioxidant and enzyme inhibitory properties of A. pseudoaleppica extracts may support the widespread ethnobotanical use of the plant application. Communicated by Ramaswamy H. Sarma
