Yazar "Aygun, Husamettin" seçeneğine göre listele

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    The effects of mucosal media on some pathogenic traits of Crohn's disease-associated Escherichia coli LF82
    (Future Medicine Ltd, 2018) Aygun, Husamettin; Karamese, Murat; Ozic, Cem; Uyar, Fikret
    Aim: Adherent-invasive Escherichia coli (AIEC) pathovar has been identified in intestinal mucosa of patients with Crohn's disease. Our aim was to compare the impact of sterile mucosal media (Muc-M) originated from different parts of the intestine on some pathogenic traits of AIEC LF82 strain. Materials & methods: Muc-M composed of certain rates of cell culture medium or M63 minimal medium and mucosal contents obtained from different part of intestine were designed for cell-infection experiments and biofilm-formation assays. Results: The results showed that Muc-M reduced usually pathogenic properties of AIEC LF82. However, LF82 adhesion, invasion and specific biofilm formations were markedly higher in Muc-MCR than those in Muc-MIR. Conclusion: In this context, the findings of present study could help the endeavors related to determining molecular targets for AIEC bacteria.
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    The Inhibitory Effect of Ileal Mucosal Media Originated from FVB/N mice strain on Escherichia coli LF82 Invasion
    (Bezmialem Vakif Univ, 2019) Aygun, Husamettin; Karamese, Murat; Uyar, Fikret
    Objective: The aim of this study was to investigate the effect of healthy mucosa on adhesive and invasive properties of AIEC reference strain Escherichia cell (E. coli) LF82. For this purpose, we had designed special medias that contained cell culture medium and mucosal content obtained from different regions (colon and ileum) of the digestive tract. Methods: We tested the infecting ability of AIEC reference strain E. coli LF82 on 1-407 cells in the presence of mucosal media (Muc-M) under in ritro conditions. Muc-M composed of certain rates of cell culture medium or M63 minimal medium and mucosal contents obtained from different part of intestine were designed for cell-infection experiments and biofilm-formation assays. Results: The result showed that the mucosal media decreased the infection percentage of E. coli LF82 strain when compared with control group. It was seen that the mucosal media originating from ileum almost completely inhibited the invasion of LF82 strain. On the other hand, it was observed that the mucosal media prepared from colon) reduced the bacterial invasion only in half the rate when compared with control. Conclusion: The findings showed that these medias obtained from different regions of the intestinal tract affected LF82 invasion at different rates. Therefore, this study provided crucial information that could contribute to the future studies on the localization of bacteria
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    The interaction methylene blue and glutathione-S-transferase purified from human erythrocytes
    (Springer Heidelberg, 2020) Uzan, Serhat; Acay, Hilal; Baran, M. Firat; Bilden, Alican; Aygun, Husamettin
    It is known that textile dyes have various risks on human health. Glutathione-S-transferase enzymes play a critical role in the detoxification of xenobiotics in living systems. This study aimed to examine the interaction of methylene blue with human erythrocyte glutathione-S-transferase purified in one step. Human erythrocyte glutathione-S-transferase was purified with approximately 750-fold purification and 30% efficiency by glutathione agarose affinity chromatography. The results showed that the enzyme was inhibited by methylene blue with an IC(50)value of 1.40 mmol/L. The Ki constant of methylene blue was 1.17 mmol/L. The Lineweaver-Burk graph of the methylene blue showed that the type of inhibition was compatible with mixed type inhibition. A new third spot was also detected by thin-layer chromatography. Furthermore, the cytotoxicity of methylene blue on human erythrocytes was evaluated and it was found that the haemolysis per cent of methylene blue on erythrocytes was approximately 14%.