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Bir kedideki nekrotizan fasiit olgusundan izole edilen iki zoonotik bakteri: Streptococcus canis ve Staphylococcus felis
(Veteriner Hekimler Derneği, 2021) Yücetepe, Ayfer Güllü; Arserim, Neval Berrin; Özcan, Nida; Cenak, Hakan; Keskin, Oktay
Nekrotizan fasiit (NF) deri altı ve fasiyal dokuların hızla yayılan, potansiyel olarak hayati tehlike yaratan bakteriyel bir enfeksiyonu olup, hastalığın etiyolojisinde farklı bakteriler yer alır. Bu bildiride bir özel veteriner kliniğine yüzde yara şikâyetiyle getirilen 2 yaşında, 3 kg ağırlığındaki dişi tekir kedinin klinik muayenesinde NF olası tanısı konulan bir olgu sunu amaçlanmıştır. Hasta kedinin klinik muayenesinde genel durumun iyi olduğu, yüz bölgesinin her iki tarafında irinleşmemiş nekrotik lezyonlar olduğu saptandığı için Harran Üniversitesi Veteriner Fakültesi Mikrobiyoloji Anabilim Dalına başvuruldu. Lezyonlu bölgelerden alınan steril svap örneklerinden %7 koyun kanlı agara ekim yapılarak aerobik ve anaearobik olarak inkübe edildi. Oluşan koloniler identifikasyona alındı. Klasik biyokimyasal testlerle Staphylococcus spp. ve Streptococcus spp. olarak saptanan etkenler MALDI-TOF’la (Bruker Corporation; Billerica, MA, USA) aynı zamanda zoonoz ajanlar olan Staphylococcus felis ve Streptococcus canis identifiye edildi. Kirby-Bauer disk difüzyon tekniğiyle yapılan antibiyotik duyarlılık testine göre Staphylococcus felis tetrasiklin, penisilin, enrofloksasin, ampisilin, gentamisin, eritromisin, vankomisin, novobiosin, amoksisilin/klavulonik asit ve imipenem’e duyarlı, neomisin ve streptomisin’e orta derecede duyarlı ve oksasilin’e dirençli bulunurken, Streptococcus canis penisilin, oksasilin, enrofloksasin, ampisilin, eritromisin, vankomisin, amoksisilin/klavulonik asit ve imipenem’e duyarlı, neomisin, tetrasiklin, novobiosin’e orta derecede duyarlı, streptomisin ve gentamisin’e dirençli olarak saptandı. Bu sonuçlara göre tedavi olarak 10 gün süre ile enrofloksasin + amoksisilin/kalvulonik asit kombinasyonu yapıldı. Tedavi, 2 hafta kantaron yağı, sonrasında aloea-vera sıvısı ve jeli, ayrıca propolisle desteklendi. Tedavi süresi sonucunda klinik iyileşme gözlendi. Sonuç olarak farklı bakteriler tarafından oluşturulabilen NF olgularında başarılı bir tedavi için, etiyolojinin ve duyarlı antibiyotiklerin belirlenmesinin hayvan ve insan sağlığı açısından önemli olduğu sonucuna varıldı.
Bir sığırcılık işletmesinde çoklu antibiyotik dirençli pseudomonas aeruginosa epidemisi
(Dicle Üniversitesi Veteriner Fakültesi, 2012) Keskin, Oktay; Tel, Osman Yaşar; Arserim, Neval Berrin
İthal holştayn ırkı sığırlardan oluşan 1000 başlık özel bir süt işletmesinde buzağılarda görülen enteritis, irinli deri yaraları, poliartritis, apse gibi klinik bozukluklar ve % 24 (60/250) oranındaki ölümler nedeniyle Harran Üniversitesi Veteriner Fakültesi Mikrobiyoloji Anabilim Dalı’na başvuruldu. Alınan klinik materyallerin bakteriyolojik muayenesi sonucunda etken P. aeruginosa olarak identifiye edildi. Elde edilen izolatın antibiyotik duyarlılığını saptamak için Kirby Bauer Disk Diffüzyon yöntemi ile ampisilin, amoksisilin, amokisilin/klavulanik asit, sefoksitin, siprofloksasin, eritromisin, gentamisin, imipenem, norfloksasin, oksasillin, penisilin, rifampin, streptomisin, tetrasiklin, trimetoprim-sulfametoksazol ve vankomisin test edildi. İmipenem duyarlı olarak saptanırken, diğer antibiyotiklerde zon oluşumu görülmedi ve dirençli olarak değerlendirildi. Sonuç olarak çoklu antibiyotik direncine sahip P. aeruginosa suşlarının neden olduğu infeksiyonların işletmelerde ciddi kayıplar oluşturabileceği, tedavide başarı sağlamak için laboratuvar muayeneleri ve antibiyotik duyarlılık testinin önemli olduğu kanısına varıldı.
Clinical and radiological evaluation of distal extremity lesions in racehorses
(Universidad del Zulia, Facultad de Ciencias Veterinarias, 2024) Kanay, Berna Ersöz; Çatalkaya, Emine; Arserim, Neval Berrin; Ketani, Muzaffer Aydın; Demirtaş, Berjan
Distal extremity lesions are prevalent in racehorses and cause mild– to–severe lameness, adversely affecting the racing life of horses. This study aimed to determine the incidence of distal extremity lesions in racehorses, to clinically and radiologically assess those lesions. The study included 158 limbs from 140 horses with distal extremity lesions among 282 horses of different breeds, age and sex that were presented to Equine Hospital of the Directorate of Diyarbakir, Turkey, Hippodrome with various clinical complaints during a racing season. Following anamnesis, the horses were clinically examined and lameness was localised. In cases where the lameness could not be localised upon physical examination, regional anaesthesia was performed, followed by radiological assessments based on radiographs acquired from different positions. Clinical and radiological examination revealed that 84.17% of the cases occurred in the forelimb and 15.82% in the rear limb, and the lesions in the forelimb were mostly located in the carpal joint (26.58%), metacarpus (25.31%) and metacarpophalangeal joint (23.41%), whereas those in the rear limb were located in the tarsal joint (7.5%). Therefore, the lesions in the forelimbs were more prevalent than those in the rear limbs of the affected racehorses. Radiography remains a satisfactory imaging technique for the diagnostic imaging of distal limb injuries in horses owing to its cost–effectiveness.
Culture- and polymerase chain reaction-based detection of flavobacterium psychrophilum in naturally infected rainbow trout (Oncorhynchus Mykiss Walbaum, 1792) from trout farms
(Hasan ÖNDER, 2023) Özcan, Filiz; Arserim, Neval Berrin; Ketani, M. Aydın; Aydın, Kasım; Sever, Nurdan Karacan; Özcan, Nida; Çelik, Burçak Aslan
The present study aimed to detect Flavobacterium psychrophilum in fish samples collected from rainbow trout (Oncorhynchus mykiss Walbaum 1792) farms in the Southeastern Anatolia region of Türkiye by means of bacteriological culture and polymerase chain reaction and to investigate the antibiotic susceptibility of the causative bacteria. A total of 40 trout farms located in Diyarbakır, Adıyaman, Şanlıurfa, and Batman provinces were visited, and 1200 samples were examined. During January and February 30 fish with an average live weight of 200–250 g were collected from each farm. Samples were obtained from the liver, spleen, kidneys, and tissues following macroscopic laboratory examination of the specimens. Antibiotic treatment is the treatment of choice owing to the lack of an effective vaccine in the control of the disease. Therefore, it is important to rapidly identify the bacterial species and investigate its susceptibility to antibiotics. In this study, the causative bacteria were detected in 5 out of 40 farms. The causative bacteria infected the liver, kidney, and tissues. The sensitivity of Enrofloxacin (5 microgram (µg)), Florfenicol (30µg), Neomycin (5µg), Amoxicillin (25µg), Oxytetracycline (30µg), Erythromycin (10µg), Gentamycin (5µg), Streptomycin (5µg) and Nalidixic acid (10µg) were defined at chancing ratios. In conclusion, these bacteria were detected in regional farms, which should minimize the stress factors by avoiding overstocking and following the required hygiene rules.
Detection of Yersinia ruckeri in naturally infected rainbow trout (Oncorhynchus mykiss Walbaum, 1792) from trout farms in Anatolia, Turkey
(Universidad del Zulia, Facultad de Ciencias Veterinarias, 2023) Özcan, Filiz; Arserim, Neval Berrin; Ketani, Muzaffer Aydın; Aydın, Kasım; Sever, Nurdan Karacan; Özcan, Nida; Çelik, Burçak Aslan
The present study aimed to detect Yersinia ruckeri bacteria in fish samples collected from the rainbow trout (Oncorhynchus mykiss, Walbaum, 1792) farms in the South Eastern Anatolia Region of Turkey through bacteriological culturing and polymerase chain reaction. Separate visits were made to trout farming facilities in Diyarbakır, Adıyaman, Şanlıurfa, and Batman in January and February 2021, and 30 fish with an average live weight of 200–250 g were collected from each facility. A total of 1,200 samples were taken from 40 trout farms. Liver, spleen, kidney, and tissue specimens were collected from rainbow trout. Yersinia ruckeri was isolated from the kidney, liver and muscle tissue of fish on McConkey Agar for bacteriological examinations. Isolated strains were identified by MALDI–TOF and PCR. It was detected the bacteria in the liver, kidney, and muscle tissue samples of fish from 23 out of 40 farms. In conclusion, these bacteria were detected in local farms. These farms must implement vaccination, minimize stress factors affecting the fish, and avoid overstocking. The best methods to protect against infection include avoiding overstocking, low oxygen levels, and underqualified labor force.
Determination of serogroup and lytic activities of bacteriophages isolated from phage plaques in Staphylococcus aureus cultures identified from sheep milk with mastitis
(Agricultural Research Communication Centre, 2024) Gürbilek, Sevil Erdenliğ; Arserim, Neval Berrin; Tel, Osman Yaşar; Sertkaya, Zeynep Tuğçe; Keskin, Oktay
Backgorund: Bacteriophages are closely related to the evolution and virulence of some important bacterial pathogens. Due to their highly significant roles in pathogenesis and virulence, S. aureus bacteriophages are frequently studied. Bacteriophages are grouped into two main categories depending on their life cycles. There are highly consistently lytic phages (virulent) and temperate phages. This study aimed to isolate bacteriophages and determine their phage serogroups from phage plaques in S. aureus cultures in order to show if they are lytic or lysogenic, the latter plays a major role in horizontal gene transfer. Methods: A total of 234 S. aureus isolates were recovered from milk samples from cases with gangrenous mastitis in sheep. Staphylococcal phages are determined based on the type and serogroup by PCR using specific primers. Result: Our study allowed us to determine serogroups of the isolated bacteriophages. Two phage stock samples included only one serogroup while the others included more than one phage serotypes and needed further purification Fa, L and D serogroups were not determined in the study. Present work revealed that all the isolated phages were temperate phages, which play a highly significant role in horizontal gene transfer.
DETERMINATION OF TETRACYCLINE GROUP ANTIBIOTICS RESIDUES IN THE TIGRIS RIVER AND AGRICULTURAL LANDS FED BY TIGRIS RIVER AND REDUCTION OF UNCONSCIOUS USE OF ANTIBIOTICS
(Publ House Bulgarian Acad Sci, 2024) Ozcan, Filiz; Celik, Aytekin; Arserim, Neval Berrin
The recent increase in environmental pollutants is contaminating important natural resources and causing irreversible damage. Due to the limitations of traditional treatment techniques, studies on the problem of micro pollutant contamination of water resources have increased significantly. Antibiotics used in medicine and veterinary medicine cause significant pollution of water resources as residues of these contaminants remain unchanged in the aqueous environment. This has recently been recognised as a global environmental health threat. The ecosystem is vulnerable to residues of these contaminants from agriculture and aquaculture. In this study, the presence of tetracycline group antibiotics, the most commonly used in veterinary medicine, was determined in the Tigris river. This is the first study to date in which antibiotics from the tetracycline group have been identified in the Tigris river. Water samples were taken from 16 different points (80 samples) along the river. Sampling points were grouped as abattoirs, livestock markets, farmland, cafes and bridges near settlements. The samples were analysed for the presence of tetracycline (TC), oxytetracycline (OTC) and chlortetracycline (CTC). With a concentration range of 0.037 to 1.756 ng/L, oxytetracycline was found in 14 of the 16 sites. A concentration of 0.004-0.014 ng/L of tetracycline was found in five samples. Chlortetracycline was found in six samples, with concentrations ranging from 0.015 to 0.097 ng/L. According to the study, the tetracycline family of antibiotics was found in the water samples, and oxytetracycline was present in the highest concentrations in most of them.
Fungal diseases in fish
(Hasan ÖNDER, 2022) Özcan, Filiz; Arserim, Neval Berrin
Fungal diseases of fish have become increasingly important, over the past 20 years. The traditional ‘‘fungi’’ are comprised of members from several different taxonomic kingdoms. An increasing number of other environmental fungi are being reported from diseased fish, further testament to the opportunistic nature of many fungi. Active fungal agents living in waters cause infections in juvenile and adult individuals in fish populations, leading to decay in eggs and larvae. Fungal infections that are generally observed as a secondary infection in fish populations become established in lesions caused as a result of mechanical injury by bacterial, viral, and parasitic primary agents, consequently causing a change in the prognosis of the disease. Fungi, however, can cause disease under a variety of other circumstances. Some may be more aggressive and play a more primary role. Fungi can be external or internal, and systemic. Fungi can cause problems during reproduction, for example, by infecting fertilized eggs in spawns. Certain species of fungi can grow in poorly stored feeds and produce mycotoxins. Fungal diseases, in general, are very difficult to control or treat once they have taken hold. Prevention is, as always, the best medicine. Increased knowledge of basic biology will help guide treatment and control methods. This review study sought to provide insights into the fungal diseases of wild or farmed fish introduced onto the market
Identification of Vibrio parahaemolyticus by PCR Method in Fish and WaterSamples from Tigris River, Turkey
(2021) Ketani, Aydın; Özcan, Filiz; Arserim, Neval Berrin
Climate change has caused increasing Vibrio parahaemolyticus infections in areas unaffected by this pathogen.Water temperature is an important ecological factor. It is consequently of high importance to follow V. parahaemolyticus inaquatic environments and foods. The aim of this work was to detect the presence of V. parahaemolyticus in Tigris Riverfrom eight different sites namely Malabadi Bridge, Fiskaya, Ongözlü Bridge, Bağıvar, Pamukçay, Ambarçay, Seyrantepe andSilvan. Total 480 samples of water and fish were used for V. parahaemolyticus detection. Thiosulphatecitrate?bile saltsucrose (TCBS) Agar was employed for bacteria isolation for 24 h at 37?C. The dominant uniform bacterial colonies werepurified by streaking onto the TCBS agar plates three times. Identification of this pathogen was made by PCR. A total of 480water and fish samples were analyzed. According to the results, V. parahaemolyticus was detected in 40 (6.6%) fish samplestaken from the Seyrantepe region.
Kene enfestasyonuna bağlı preseptal selülit
(2010) Şakalar, Yıldırım Bayezit; Ünlü, Kaan; Keklikçi, Uğur; Balsak, Selahattin; Alakuş, Mehmet Fuat; Arserim, Neval Berrin
Dört yaşında kız çocuğu başında ve sol üst göz kapağı üzerinde kene ısırığı şikayeti ile başvurdu. Kene ısırıkları bir gün önce hastanın ailesi tarafından fark edilmişti. Muayenede sağ üst göz kapağı kirpik kenarında kene enfestasyonu saptandı. Her iki gözde kapaklar ödemliydi ve sol göz kapağı hafif hiperemikti. Künt uçlu bir forseps yardımıyla, uygun teknikle kene çıkartıldı ve profilaktik sistemik antibiyotik tedavisi başlandı. Olguda yüksek ateş ve kanama pıhtılaşma zamanlarında uzama gibi bulgular izlenmedi. Mikroskobik incelemede kenenin Ixodes türleri olduğu saptandı. Her iki göz kapağındaki ödem 3 gün sonra düzeldi ve sistemik herhangi bir komplikasyon gözlenmedi. Serolojik incelemelerde Kırım Kongo Kanamalı ateşi yönünden etken tespit edilmedi.
Preseptal Cellulitis Related to Tick Infestation
(Turkish Ophthalmological Soc, 2010) Sakalar, Yildirm Bayezit; Arserim, Neval Berrin; Keklikci, Ugur; Balsak, Selahattin; Alakus, Mehmet Fuat; Unlu, Kaan
A 4-year-old girl presented with complaint of tick bite on the left upper eyelid and head. The tick bites were noticed by her parents one day ago. On her examination, tick infestation was found in the lash margin of the right upper eyelid. Bilateral eyelid edema and mild hyperemia of the left upper yelid were observed. The tick in the eyelid was removed with a proper technique using a blunt-ended forceps and prophylactic systemic antibiotic therapy was started. Signs such as high fever or prolongation of clotting time were not observed in the patient. The species of tick was identified as Ixodes species on microscopic examination. The edema of both eyelids resolved 3 days later and no systemic complications were observed. The Crimean Congo hemorrhagic fever was not detected on serological investigations.
Prevalence of Four Enteropathogens with Immunochromatographic Rapid Test in the Feces of Diarrheic Calves in East and Southeast of Turkey
(Univ Agriculture, Fac Veterinary Science, 2013) Icen, Hasan; Arserim, Neval Berrin; Isik, Nurettin; Ozkan, Cumali; Kaya, Abdullah
In this study, fecal specimens taken from 192 diarrheic and 14 healthy calves (2-40 days old) were examined for the presence of bacterial and parasitic agents. Fecal samples from diarrheic calves with the four immunochromatographic rapid tests were 92.7% positive for four enteropathogens. The individual prevalence was 25, 21.8, 9.4 and 2.1% for Rotavirus, Cryptosporidium parvum, E. coli K99 and Coronavirus, respectively. Concomitant infections caused by two agents were 15.6% for Rotavirus+Cryptosporidium, 1.0% for Rotavirus+Coronavirus, 5.2 % for Cryptosporidium+E. coli K99, and 7.3% for Rotavirus+E. coli K99. Besides concomitant infections caused by three agents were 3.1% for Cryptosporidium +Rotavirus+E. coli K99 and 1.0%, Cryptosporidium+Rotavirus+Coronavirus. In addition one calf (1.0%) was infected by combination of four agents as Cryptosporidium, Rotavirus, Coronavirus, and E. coli K99. The calculated individual prevalence was 56.9% for Rotavirus, 47.8% for C. parvum, 26.0% for E. coli K99 and 5.2% for Coronavirus. However, 88 samples were positive in smear detection for Cryptosporidium while 92 were positive in rapid test. As a result of this study it can be concluded that multiple etiologies of diarrhea can be seen and this can help in the development of a specific treatment and preventative measures for practitioners in east and southeast of Turkey. (C) 2013 PVJ. All rights reserved
Preventive effect of fucoxanthin administration on intra-abdominal adhesion: An experimental animal study
(Turkish Assoc Trauma Emergency Surgery, 2022) Dalbasi, Erkan; Tuzun, Abidin; Arserim, Neval Berrin; Ozcan, Filiz; Dogan, Elif; Cetin, Alpay
BACKGROUND: The most common cause of intra-abdominal adhesion (IAA) is previous abdominal surgery and mortality. IAA can cause serious complications such as chronic abdominal pain, ileus, and infertility. Approximately 3% of all laparotomies are related to adhesions. IAA reduces the quality of life of the patient, causes morbidity, and increases health expenditures. In this study, we aimed to investigate the preventive effect of fucoxanthin (Fx) on IAA in the intra-abdominal surgical adhesion model that experimentally created in rats. METHODS: This study used 21 Sprague-Dawley rats divided into three groups. After anesthesia, the abdomen was opened, the cecum and right abdominal wall were damaged with a sterile toothbrush until petechiae bleeding was seen. No additional action was taken to the control group. In the sham group, 5 cc saline solution was released into the peritoneum before the abdomen was closed. In the Fx group, 35 mg/kg Fx was instilled intraperitoneally and the abdomen was closed. On the 21st post-operative day, all subjects were anesthetized with standard anesthesia. Macroscopic adhesions were quantitatively evaluated according to the Mazuji classification. The cecum anterior wall and parietal peritoneum were excised for pathological sampling. A pathologist, unaware of the groups, evaluated inflammation, fibroblastic activity, and vascular proliferation. In addition, serum tumor necrosis factor-alpha (TNF-alpha) and interleukin-10 levels were measured. RESULTS: No rat was lost during the study period. Congenital adhesion was not observed in any of the subjects at the first laparotomy. Adhesion was significantly less macroscopically in the Fx group compared to the control and sham group (p<0.001 and p<0.001). Fibroblastic activity was found to be significantly less in the Fx group compared to the sham and control groups (p<0.001 and p<0.001). Vascular proliferation was found to be significantly less in the Fx group than in the sham and control groups (p<0.001 and p<0.001). The inflammation score was significantly lower in the Fx group compared to the other two groups (p<0.001 and p<0.001). The inflammation score in the sham group was lower than the control group and was statistically significant (p<0.001). TNF-alpha level was found to be statistically significantly lower in the Fx group compared to the sham and control groups (p<0.001 and p<0.001). CONCLUSION: As a result of experimental study, we can say that Fx is effective in preventing IAAs and decreases the level of TNF-alpha, a pro-inflammatory cytokine.
THE RELATIONSHIP BETWEEN H. PYLORI AND DIETARY HABITS IN OBESE FEMALE PATIENTS
(Publ House Bulgarian Acad Sci, 2022) Karaman, Ulku; Kaya, Yasemin; Arici, Yeliz kasko; Arserim, Neval Berrin; Karagoz, Zuhal Karaca; Colak, Cemil; Yolalan, Gamze
The purpose of this study is to investigate the relationship between the dietary habits of obese women aged above 18 and the Helicobacter pylori (H. pylori). Five hundred and sixty-one women aged above 18 were included in the study. Patients filled in questionnaires consisting of questions regarding their sociodemographic variables, chronic diseases and dietary habits. Blood samples taken from the patients were tested according to the H. pylori kit procedures and the results were categorized as positive and negative. In the study, obese and nonobese patients were compared and then H. Pylori positive and negative groups were compared. No significant difference was found between groups in terms of H. pylori in the comparison of obese and nonobese patients (p = 0.272). When H. pylori positive and negative groups of obese patients were compared in terms of their dietary habits, consumption rates of butter, whole-fat or lowfat milk, cookies-cake etc., and chocolate-candies were detected to be higher in the H. pylori positive group (p < 0.05). Red meat consumption (p = 0.044) and smoking rates were lower (p = 0.001). There was no significant difference between groups in terms of the consumption rates of bread, rice/pasta, corn, cheese, chicken, fish, sausages, fruit, vegetables, legumes and salt. No significant difference was found between obese and nonobese women in terms of H. pylori positivity. It was observed that H. pylori positive obese women had higher consumption rates of butter, whole-fat or low-fat milk, cake-cookies etc., and chocolate-candies.
The Relationship between Helicobacter Pylori and intestinal parasites in patients with peptic ulcer
(2023) Kaya, Yasemin; Karaman, Ülkü; Çolak, Cemil; Çınar, Hamza; Karataş, Ahmet; Arserim, Neval Berrin; Yolalan, Gamze
Aim: This study aimed to evaluate the frequency of Helicobacter pylori (H. pylori), risk factors, and co-infection with intestinal parasites in adult patients presenting gastrointestinal complaints. Materials and Methods: The working group of the study consisted of 385 patients with gastrointestinal complaints. A questionnaire including questions aiming to canvass the socio-demographic features, lifestyles, and complaints of the patients was administered to the study population. Cellophane slide method, native-lugol, sedimentation and Modified kinyoun acid-fast methods were used for the diagnosis of parasites in stool, under microscope. The H. pylori antigen was studied in the stool sample taken for the diagnosis of H. pylori.Results: H. pylori positivity was found to be 27.79% in the patients included in the study. 76.6% of those who are positive for H. pylori are women, and the positivity rate was found to be higher at the age of 40 and over (75.7%). The majority of patients with H. pylori positivity expressed being married (73.8%), having middle / low-income (89.7%), having a low educational background (82.2%), living in a village (55.1%), and in a nuclear family (72.2%) (p<0.001). H. pylori positivity was higher in those who used tap water (40.2%) and those who had a vegetable-based diet (75.7%) (p<0.001). The study found a statistically significant correlation between Entamoeba histoloytica and Enterobius vermicularis positivity and H. pylori positivity (p<0.05 p<0.001, respectively). The calculated odds ratio showed that H. pylori positivity was 1.19 times higher in Entamoeba histoloytica positivity and 11.27 times higher in Enterobius vermicularis positivity. Conclusion: Larger and more comprehensive studies should be performed to understand better the epidemiology, clinical effects, treatment, and control of H. pylori co-infection.
Seroprevalance of Coxiellosis in cows, sheep, goats and humans in Diyarbakir region of Turkey
(Academic Journals, 2011) Arserim, Neval Berrin; Yesilmen, Simten; Tel, Osman Yasar; Ozekinci, Tuncer; Keskin, Oktay; Pulat, Huseyin; Vural, Aydin
This study aims at determining the seroprevalance of Coxiella burnetii in cows, sheep, goats and staff, working in the stock breeding sector in Diyarbakir region. Therefore, C. burnetii antibodies were investigated in sera samples of 612 sheep, 700 goats, 584 cows and 90 staff by enzyme-linked immunosorbent assays (ELISA). In the study, seropositivity was obtained as 25.4, 38.6, 20.0 and 6.6% in sheep, goats, cows and stockbreeding staff, respectively. Consequently, C. burnetii seropositivity, whether in people or in animals, had a ratio that should not be ignored in Diyarbakir region. Abort cases in ruminant should be assessed from the viewpoint of Coxiellosis. Also, people, especially those who are in risk group, should be made to be conscious of Coxiellosis infection, and measures for preventing this illness should be taken.
Smooth and rough brucella infections in dogs
(Agricultural Research Communication Centre, 2024) Arserim, Neval Berrin; Gürbilek, Sevil Erdenliğ; Keskin, Oktay; Tel, Yaşar; Doğan, Elif
Background: B. canis infections have been reported in many countries of the world. B. canis can cause infections both in dogs and human beings. It can be transmitted to human via laboratory accidents during handling and contact with sick dogs. is an infectious agent for humans and it is transmitted to humans by various ways including laboratory accidents and contact with sick dogs. Methods: In this study serum samples were collected by using stratified random sampling method from the Diyarbak & imath;r Metropolitan Municipality Animal Care and Rehabilitation Center, where approximately 600 dogs were housed. Result: Out of 83 samples , 9.6% and 26.5% of serum samples were found as positive by B.canis RSAT and B. canis ELISA, respectively. While 6% and 9.6% of the samples were positive by B. abortus S99 RSAT and B. abortus ELISA, respectively. Sensitivity and specificity of B. abortus S99 ELISA was 98% and 95.5%, respectively. On the other hand, sensitivity and specificity of B.canis M (-) ELISA was 96.6% and 94%. As a result, it was concluded that B. canis infection is common in dogs and this should be considered both for dogs and public health. Unfortunately, there is no standardized commercial serological test for diagnosis of B.canis and other rough Brucella species currently. This is the main reason that rough brucella infections might not represent the real B.canis infection situation. Therefore, most of the cases have the possibility of being undetected. For this reason it is utmost important that B.canis RSAT and ELISA can be used as routine tests in the hospitals and veterinary clinics in the serological diagnosis of brucellosis.
Some Production Characteristics of Zom Sheep Types
(Academic Journals Inc, 2012) Celik, Romedi; Arserim, Neval Berrin; Durak, M. Hanifi
This study was done to determine fertility, growth and body measurements in Zom sheep type reared in the vicinity of Diyarbakir. 96 Zom sheep and 107 lambs, born from them were used. Fecundity, fertility and twinning (%) were 1.11, 1.13 and 13.8%, respectively. Birth weight, 15th, 30th, 45th, 60th days at body weight and daily live weight gain from birth to 60 days age for Zom lambs were 4.75, 9.34, 12.85, 15.89, 18.70 kgs and 232 g, respectively. While dam age effective on birth weight, 15 and 30th day of body weight of lambs, gender was found significant on whole growth period from birth to 60 days of age and daily live weight gain (p<0.05). The average body size of Zom sheep were determined as these, respectively; withers height 72.89 cm, body length 75.05 cm, chest depth 34.97 cm, chest width 25.29 cm, chest circumference 105.42 cm, ear length 16.85 cm and front shin circumference 8.66 cm.
Veteriner epidemiyoloji’de sistematik derleme ve meta-analizi
(Dicle Üniversitesi Veteriner Fakültesi, 2012) Arserim, Neval Berrin; Keskin, Oktay
Meta-analizi, belirli bir konuda farklı yer ve zamanlarda gerçekleştirilmiş birbirinden bağımsız çok sayıda çalışmanın sonuçlarını niteliksel ve niceliksel olarak birleştirmeye ve o konuda ortak bir yargıya ulaşmaya yardımcı olan istatistiksel bir yöntemdir. Bu çalışmada veteriner mikrobiyolojide belirlenen problemlerin çözümünde meta-analiz yönteminin kullanılması amaçlanmıştır. Bu amaçla Kırım Kongo Kanamalı Ateşi virüsünün çiftlik hayvanlarında varlığı ve atlarda Taylorella equigenitalis’in kültür ve PCR metotlarıyla tespitinde sonuçların tutarlılığının incelenmesi olarak iki problem belirlenmiştir. Araştırmaya yönelik Dicle Üniversitesi Kütüphanesi’nin üyesi olduğu veri tabanları (Pub Med, Science Direct, ISI Web, Proquest, Summon vb.) kullanılmıştır. Her iki hipotezde belirlenen literatürler, kitap ve/veya bölümleri, İngilizce olmayan literatürler, derleme ve tekrarlar ayıklandıktan sonra okunarak meta analizinde kullanılacak verilerin elde edilmesine çalışıldı. Ancak her iki hipotezde de meta-analizi yapılabilecek veriler elde edilemedi. Bunun sebepleri olarak kısaca hayvan türlerinin farklılığı, çalışma metotlarının çeşitliliği ve istatistiki metotların aynı olmamasından bahsedilebilinir. Sonuç olarak, veteriner hekimliğini ve hayvan sağlığını ilgilendiren çalışmalarda daha yaygın olarak sistematik derleme ve meta-analizi uygulayabilmek için yayınlanan makalelerde yukarıda bahsedilen hususlara dikkat edilmesi gerektiği anlaşılmıştır.

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