Culture- and polymerase chain reaction-based detection of flavobacterium psychrophilum in naturally infected rainbow trout (Oncorhynchus Mykiss Walbaum, 1792) from trout farms
Yükleniyor...
Tarih
2023
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Hasan ÖNDER
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The present study aimed to detect Flavobacterium psychrophilum in fish samples collected from rainbow trout
(Oncorhynchus mykiss Walbaum 1792) farms in the Southeastern Anatolia region of Türkiye by means of bacteriological culture and
polymerase chain reaction and to investigate the antibiotic susceptibility of the causative bacteria. A total of 40 trout farms located in
Diyarbakır, Adıyaman, Şanlıurfa, and Batman provinces were visited, and 1200 samples were examined. During January and February
30 fish with an average live weight of 200–250 g were collected from each farm. Samples were obtained from the liver, spleen, kidneys,
and tissues following macroscopic laboratory examination of the specimens. Antibiotic treatment is the treatment of choice owing to
the lack of an effective vaccine in the control of the disease. Therefore, it is important to rapidly identify the bacterial species and
investigate its susceptibility to antibiotics. In this study, the causative bacteria were detected in 5 out of 40 farms. The causative
bacteria infected the liver, kidney, and tissues. The sensitivity of Enrofloxacin (5 microgram (µg)), Florfenicol (30µg), Neomycin (5µg),
Amoxicillin (25µg), Oxytetracycline (30µg), Erythromycin (10µg), Gentamycin (5µg), Streptomycin (5µg) and Nalidixic acid (10µg)
were defined at chancing ratios. In conclusion, these bacteria were detected in regional farms, which should minimize the stress factors
by avoiding overstocking and following the required hygiene rules.
Açıklama
Anahtar Kelimeler
Antibiotic susceptibility, Bacterial cold-water disease, Flavobacterium psychrophilum
Kaynak
Black Sea Journal of Agriculture
WoS Q Değeri
Scopus Q Değeri
Cilt
6
Sayı
6
Künye
Özcan, F., Arserim, N. B., Ketani, M. A., Aydın, K., Sever, N. K., Özcan, N. ve diğerleri. (2023). Culture- and polymerase chain reaction-based detection of flavobacterium psychrophilum in naturally infected rainbow trout (Oncorhynchus Mykiss Walbaum, 1792) from trout farms. Black Sea Journal of Agriculture, 6(6), 655-659.
