Yazar "Akdeniz, Mehmet" seçeneğine göre listele
Listeleniyor 1 - 20 / 34
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Anadoluda Yetişen Dört Centaurea L. Türünün Antiaging ve Antidiyabet Etkilerinin Belirlenmesi(Hakkari University, 2023) Çavuşoğlu, Mehmet; Akdeniz, Mehmet; Yener, İsmail; Alkan, Hüseyin; Ertaş, AbdulselamCentaurea L., dünya çapında, özellikle Batı Asya ve Akdeniz bölgelerinde dağılım gösteren 600'den fazla türüyle Asteraceae familyasının dördüncü en büyük cinsi olup Türkiye'de 109'u endemik olmak üzere 179 türü bulunmaktadır. Bu çalışmada, Anadolu'dan dört Centaurea türünün antiaging ve antidiyabet etkilerini belirlemesi amaçlanmaktadır. Ayrıca Centaurea lycopifolia, C. balsamita, C. depressa, ve C. iberica türlerinin GC-MS ile bazı triterpen içerikleri belirlenmiştir. C. lycopifolia türünün aseton ekstresinde (4271.54 µg/g ekstre), C. balsamita türünün aseton ekstresinde (2872.28) ve metanol ekstresinde (3564.05) ?-amirin tespit edilmiştir. Antiaging açısından elastaz ve kollajenaz yöntemlerinde C. lycopifolia aseton ekstresinin elastaz enzimine karşı iyi derecede (27.83±1.31, oleanolik asit:37.47±0.82) inhibe edici aktivite sergilemiştir. Antidiyabetik açısından değerlendirdiğimizde ise C. depressa türünün aseton ekstresinin 12.5, 50 ve 200 µg/mL farklı konsantrasyonlarda ?-glukozidazı çok yüksek düzeyde (sırasıyla, % inhibisyon: 29.03±0.49, 45.87±1.06, 84.81±1.22, akarboz: 1.23±0.03, 28.51±0.44, 64.37±1.80) inhibisyon aktivitesi gösterdiği belirlenmiştir. C. depressa türünün metanol ekstresi ile C. iberica ve C. lycopifolia türlerinin aseton ekstreleriinin ?-glukozidaz enzimini yüksek düzeyde inhibe ettiği belirlenmiştir. C. balsamita türünün metanol ekstresinin ise 800 µg/mL konsantrasyonda ?-amilaz enzimine karşı yüksek düzeyde (65.43±1.40, akarboz: 67.18±1.73) inhibisyon aktivitesi gösterdiği tespit edilmiştir. Sonuç olarak C. lycopifolia türünün antiaging potansiyeli nedeniyle kozmetik endüstrisinde, C. depressa, C. iberica, C. lycopifolia ve C. balsamita türlerinin antidiyabetik potansiyelleri nedeniyle farmasötik alanda kullanılabilmesi için daha detaylı çalışmalara konu olması gerektiği düşünülmektedir.Öğe Bioguided isolation of secondary metabolites from Salvia cerino-pruinosa Rech. f. var. cerino-pruinosa(ACG Publications, 2021) Ertaş, Abdulselam; Çakırca, Hatice; Yener, İsmail; Akdeniz, Mehmet; Fırat, Mehmet; Topçu, Gülaçtı; Kolak, UfukAbstract: In the current study, the ethanol extracts prepared from the aerial parts and roots of an endemic species, Salvia cerino-pruinosa Rech. f. var. cerino-pruinosa were fractionated on silica gel columns and tested for determination of their antioxidant activity using DPPH free radical and ABTS cation radical scavenging, and cupric reducing antioxidant capacity (CUPRAC) test assays. Twenty known secondary metabolites were isolated from the active antioxidant fractions; rosmarinic acid (1), chlorogenic acid (2), caffeic acid (3), 4- hydroxybenzoic acid (4), benzoic acid (5), luteolin 7-O-glucoside (6), bis-(2-ethylhexyl)benzene-1,2- dicarboxylate (7), salvianolic acid A (8), salvianolic acid B (9), 7-acetylroyleanone (10), 6,7-dehydroroyleanone (11), ferruginol (12), inuroyleanol (13), 12-hydroxy-6,7-secoabieta-8,11,13-triene-6,7-dial (14), ursolic acid (15), oleanolic acid (16), taraxasterol (17), lupenone (18), β-sitosterol (19), and stigmasterol (20). Rosmarinic acid, which was obtained from the aerial parts, was found to be the best antioxidant compound among the isolated secondary metabolites in DPPH free radical and ABTS cation radical scavenging, and CUPRAC assays (IC50: 1.20±0.04 µg/mL, IC50: 1.74±0.06 µg/mL, A0.5: 1.22±0.02 µg/mL, respectively). Chlorogenic and caffeic acids, luteolin 7-O-glucoside, salvianolic acids A and B, and inuroyleanol exhibited also high antioxidant activity in the mentioned assays.Öğe Bioguided Isolation of Secondary Metabolites fromSalvia cerino-pruinosa Rech. f. var. cerino-pruinosa(2021) Kolak, Ufuk; Ertas, Abdulselam; Firat, Mehmet; Cakirca, Hatice; Topcu, Gulacti; Yener, Ismail; Akdeniz, MehmetIn the current study, the ethanol extracts prepared from the aerial parts and roots of an endemicspecies, Salvia cerino-pruinosa Rech. f. var. cerino-pruinosa were fractionated on silica gel columns and testedfor determination of their antioxidant activity using DPPH free radical and ABTS cation radical scavenging, andcupric reducing antioxidant capacity (CUPRAC) test assays. Twenty known secondary metabolites were isolatedfrom the active antioxidant fractions; rosmarinic acid (1), chlorogenic acid (2), caffeic acid (3), 4- hydroxybenzoic acid (4), benzoic acid (5), luteolin 7-O-glucoside (6), bis-(2-ethylhexyl)benzene-1,2- dicarboxylate (7), salvianolic acid A (8), salvianolic acid B (9), 7-acetylroyleanone (10), 6,7-dehydroroyleanone(11), ferruginol (12), inuroyleanol (13), 12-hydroxy-6,7-secoabieta-8,11,13-triene-6,7-dial (14), ursolic acid(15), oleanolic acid (16), taraxasterol (17), lupenone (18), ?-sitosterol (19), and stigmasterol (20). Rosmarinicacid, which was obtained from the aerial parts, was found to be the best antioxidant compound among theisolated secondary metabolites in DPPH free radical and ABTS cation radical scavenging, and CUPRAC assays(IC50: 1.20±0.04 ?g/mL, IC50: 1.74±0.06 ?g/mL, A0.5: 1.22±0.02 ?g/mL, respectively). Chlorogenic and caffeicacids, luteolin 7-O-glucoside, salvianolic acids A and B, and inuroyleanol exhibited also high antioxidantactivity in the mentioned assays.Öğe Biological and chemical comparison of natural and cultivated samples of satureja macrantha C.A.Mey(ACG Publications, 2021) Akdeniz, Mehmet; Yener, İsmail; Ertaş, Abdulselam; Fırat, Mehmet; Reşitoğlu, Barış; Haşimi, Nesrin; Kandemir, Sevgi İrtegün; Yılmaz, Mustafa Abdullah; Demirköz, Aslı Barla; Kolak, Ufuk; Öksuz, SevilAbstract: In this study, investigation on the essential oils and ethanol extracts of naturally grown and cultivated Satureja macrantha samples were reported. The essential oil, flavour and terpenoid-steroid-flavonoid contents of S. macrantha samples were determined by GC-MS and their phenolic contents by LC-MS/MS. Besides, the biological activities of the samples were investigated for their antioxidant, anti-Alzheimer, antimicrobial, cytotoxic, antityrosinase, antiurease, antielastase and anticollagenase properties. The phenolic content and antioxidant capacity of the cultivated sample were higher than those of the naturally grown sample. According to the GC-MS results, terpinene-4-ol (30.9%) and p-cymene (56.7%) were determined as the major components in the essential oils of the naturally grown and cultivated S. macrantha, respectively. The flavour analysis results showed that cis-sabinene hydrate (20.7%) and carvacrol (42.2%) were found to be the major components in the naturally grown and cultivated samples, respectively. While the naturally grown sample was rich in abietane diterpenoids (ferruginol (17.5 mg analyte/g extract) and sugiol (4.2 mg analyte/g extract)), these components were not detected in the cultivated sample. The rosmarinic acid content (0.20 and 24.87 mg analyte/g extract, respectively) of the cultivated sample was found to be significantly higher than that of the natural sample. The biological activities of the samples were determined to be changed in parallel with their chemical contents that are due to factors such as climatic conditions, and soil structure.Öğe Biological and Chemical Comparison of Natural and CultivatedSamples of Satureja macrantha C.A.Mey.(2021) Akdeniz, Mehmet; Hasimi, Nesrin; Ertas, Abdulselam; Oksuz, Sevil; Demirkoz, Asli Barla; Resitoglu, Baris; Yener, IsmailIn this study, investigation on the essential oils and ethanol extracts of naturally grown and cultivatedSatureja macrantha samples were reported. The essential oil, flavour and terpenoid-steroid-flavonoid contents of S.macrantha samples were determined by GC-MS and their phenolic contents by LC-MS/MS. Besides, the biologicalactivities of the samples were investigated for their antioxidant, anti-Alzheimer, antimicrobial, cytotoxic,antityrosinase, antiurease, antielastase and anticollagenase properties. The phenolic content and antioxidant capacityof the cultivated sample were higher than those of the naturally grown sample. According to the GC-MS results,terpinene-4-ol (30.9%) and p-cymene (56.7%) were determined as the major components in the essential oils of thenaturally grown and cultivated S. macrantha, respectively. The flavour analysis results showed that cis-sabinenehydrate (20.7%) and carvacrol (42.2%) were found to be the major components in the naturally grown and cultivatedsamples, respectively. While the naturally grown sample was rich in abietane diterpenoids (ferruginol (17.5 mganalyte/g extract) and sugiol (4.2 mg analyte/g extract)), these components were not detected in the cultivatedsample. The rosmarinic acid content (0.20 and 24.87 mg analyte/g extract, respectively) of the cultivated samplewas found to be significantly higher than that of the natural sample. The biological activities of the samples weredetermined to be changed in parallel with their chemical contents that are due to factors such as climatic conditions,and soil structure.Öğe Chemical compositions by LC-MS/MS and GC-MS and biological activities of Chenopodium album subsp. album var. microphyllum(Elsevier, 2019) Yilmaz, Pelin Koseoglu; Ertas, Abdulselam; Akdeniz, Mehmet; Avci, Mine Kocyigit; Kolak, UfukChenopodium species have been used in folk medicine and as vegetable for years. In the present study, the total phenolic and flavonoid contents, biological activities, phenolic constituents and fatty acid profile of Chenopodium album subsp. album var. microphyllum (Boen.) Aellen were determined for the first time. The antioxidant effects were investigated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and cupric ion reducing antioxidant capacity assays (CUPRAC). The Ellman method was applied for the determination of the cholinesterase inhibition activity. The phenolic constituents of the methanol extract and the fatty acid profile of the n-hexane extract were evaluated by LC-MS/MS and GC-MS, respectively. Acetone and methanol extracts showed similar DPPH free radical scavenging activities (0.68 +/- 0.07 and 0.68 +/- 0.05 mmol Trolox/g extract, respectively), whereas the cupric ion reducing capacity of the acetone extract was the highest (0.41 +/- 0.05 mmol Trolox/g extract). Acetone and methanol extracts had moderate butyrylcholinesterase inhibitory activities as 65.29 +/- 1.56% and 52.64 +/- 2.78%, respectively, whereas non of the extracts possessed anti-acetylcholinesterase effect. The methanol extract was found to contain significant amounts of hesperidin (9769.13 +/- 158.26 mu g/g extract) and rutin (2935.19 +/- 39.92 mu g/g extract). The major fatty acid constituents of the n-hexane extract were identified as myristic acid (18.26%) and cis-10-pentadecanoic acid (15.39%).Öğe Comparison of chemical and biological properties of in vivo and in vitro samples of Salvia siirtica Kahraman, Celep & Dogan extracts prepared with different solvents(Elsevier, 2021) Fidan, Hilal Saruhan; Kilinc, Fatih Mehmet; Yilmaz, Mustafa Abdullah; Akdeniz, Mehmet; Yener, Ismail; Firat, Mehmet; Onay, AhmetThe use of Salvia species among the public and their importance in the scientific world increase due to their numerous pharmacological and biological activities on a daily basis. In this study, the phytochemical contents of different parts (root, branch, leaf, flower, whole) of different specimens of Salvia siirtica (SS) (in vivo and in vitro) extracts prepared with various solvents (petroleumether, chloroformand ethanol) were determined using different techniques (GC-MS and LC-MS/MS) and the results were compared. In addition, biological activities (antioxidant, cytotoxic, anticholinesterase antiurease, antityrosinase, antielastase and anticollagenase) of all samples were determined and compared. The antioxidant potential of the analysed samples was found to be high, and their enzyme activity potential was low. Besides, in vitro SS-TIS (Temporary Immersion System) sample showed high cytotoxic activity (viability% 2.12 +/- 0.06) against MCF-7 (breast cancer) cell line. The results of GC-MS and LC-MS/MS analyses indicated that ferruginol and sugiol could be isolated from the ethanol extract of S. siirtica roots, salvigenin and beta-sitosterol from the chloroform extract of the aerial parts, and phenolic compounds from the ethanol extract of the aerial parts. In addition, the amount of rosmarinic acid, caffeic acid and 12-demethylmulticauline of in vitro samples were found to be higher than those of in vivo samples. Furthermore, all samples, both in vivo and in vitro, contained high levels of rosmarinic acid and beta-sitosterol. The whole chloroform extract (SSWC) could be the source extract for salvigenin (33952.13 mu g/g) and beta-sitosterol (16369.71 mu g/g), and the root ethanol extract (SSRE) for ferruginol (17721.99 mu g/g). As a result, it is understood that the nature of the plant material, the choice of an appropriate solvent and the parts of the material used are quite effective in chemical content. S. siirtica promise potential natural antioxidant agent in food and/or pharmaceutical industry due to its phytochemical content (rosmarinic and caffeic acids, ferruginol, salvigenin) and antioxidant activity. (C) 2021 Published by Elsevier B.V. on behalf of SAAB.Öğe A comprehensive LC-MS/MS method validation for the quantitative investigation of 37 fingerprint phytochemicals in Achillea species: A detailed examination of A. coarctata and A. monocephala(Elsevier Science Bv, 2018) Yilmaz, Mustafa Abdullah; Ertas, Abdulselam; Yener, Ismail; Akdeniz, Mehmet; Cakir, Oguz; Altun, Muhammed; Demirtas, IbrahimThe current study aims to optimize and validate a comprehensive LC-MS/MS method for the quantification of 37 phytochemicals (15 phenolic acids, 17 flavonoids, 3 non-phenolic organic acids, 1 phenolic aldehyde and 1 benzopyrene) in Achillea species. Though Achillea species were chosen as real life samples, the current method is applicable to a wide range of plant species. The developed method was fully validated in terms of linearity, accuracy (recovery), inter-day and intra-day precision (repeatability), limits of detection and quantification (LOD/LOQ) and relative standard uncertainty (U% at 95% confidence level (k = 2)). Reversed-phase ultrahigh performance liquid chromatography was optimized to achive optimum separation for 37 phytochemical compounds and to overcome the suppression effects. MS detection was performed using a triple quadrupole mass spectrometer and negative or positive ionization modes were optimized for each analyte. Multiple reaction monitoring (MRM) was used to quantify the analytes, related molecular ions and transition ions were optimized. Phytochemical screening of ethanol and methanol-chloroform extracts of root and aerial parts of A. coarctata and A. monocephala were performed by using the developed and validated LC-MS/MS method. Root and aerial parts of both species have considerable amounts of certain phenolic-nonphenolic acids (quinic, malic, fumaric, chlorogenic and vanillic acids) and flavonoids (rutin, hesperidin, isoquercitrin, apigetrin, luteolin, apigenin). Additionally, total phenolic and flavonoid amounts, antioxidant (DPPH free radical scavenging assay, ABTS radical cation decolorization assay, beta-carotene lipid peroxidation test system and CUPRAC cupper reduction capacity methods), anticholinesterase, tyrosinase, urease inhibition and cytotoxic activities (on HeLa (Human Cervical Carcinoma Cell Line) of A. coarctata and A. monocephala were also investigated. It has been determined that the studied Achillea species, that are rich in total phenolic-flavonoid and chlorogenic acid contents, have high antioxidant and cytotoxic potential at the same time. According to the results of LC-MS/MS, antioxidant and cytotoxic activity studies, after detailed chemical investigation and toxicity studies on these species, A. coarctata and A. monocephala may be promoted as promising sources of natural agents and used for the development of nutraceuticals or functional food ingredients in future. (C) 2018 Elsevier B.V. All rights reserved.Öğe Comprehensive study of chemical composition and biological activity of Thymus pubescens Boiss. et Kotschy ex Celak(Elsevier, 2022) Yigitkan, Serkan; Akdeniz, Mehmet; Yener, Ismail; Seker, Zeki; Yilmaz, Mustafa Abdullah; Firat, Mehmet; Kavak, Deniz EvrimThe use of Thymus species amongst the public and their importance in the scientific world is increasing day by day. In addition to being consumed as tea and spice, Thymus species are used as folk medicine for respiratory, digestive, skin, circulatory, genital, nervous, visual and urinary diseases. In this study, it was aimed to examine the essential oil and ethanol extract of the root and aerial parts of Thymus pubescens Boiss. et Kotschy ex Celak in the terms of their biological activity and chemical content. The phenolic content of the species was determined by LC-MS/MS, while triterpenoid content, the chemical composition of the essential oil as well as flavour was determined by GC-MS. In addition, their antioxidant and cytotoxic activities, as well as acetyl- (AChE) and butyrylcholinesterase (BChE), urease, tyrosinase, elastase, collagenase, HMG-CoA reductase and angiotensin-converting enzyme (ACE) inhibitory activities were studied. Thymol (53.33%) was determined as the major component in the essential oil, while b-fenchyl alcohol (43.87%) was determined as the major component of the flavour. According to the LC-MS/MS and GC-MS results, it was found that especially the aerial parts of the species have a high amount of rosmarinic acid (3875.76 mg analyte/g extract), quinic acid (2392.55), naringenin (970.39), oleanolic (92785.96) and ursolic (63373.32) acids. The essential oil of T. pubescens species was observed to show high activity in four antioxidant assays, e.g. inhibition of lipid peroxidation, DPPH and ABTS radical scavenging activity as well as CUPRAC, while the ethanol extracts showed moderate antioxidant activity. In enzyme inhibition assays, the aerial parts exerted marked BChE, elastase, and collagenase inhibitory activities (92.43 +/- 1.28%, 42.59 +/- 0.56, and 48.61 +/- 0.39 at 100 mg/mL, respectively). On the other hand, AChE, urease, tyrosinase, HMG-CoA reductase and ACE inhibitory activities of all extracts were from low to moderate levels. In particular, the aerial parts of the species displayed a high cytotoxic effect (vitality%: 6.82 +/- 0.01 at 200 mg/mL) in breast cancer (MCF-7) cell line. Due to its remarkable antioxidant capacity, high content of rosmarinic, oleanolic, and ursolic acids and especially BChE, elastase, and collagenase inhibitory activity, T. pubescens has the potential for using in food supplements, food preservatives, cosmetics, and pharmaceutical industries.(c) 2022 SAAB. Published by Elsevier B.V. All rights reserved.Öğe A comprehensive study on chemical and biological investigation of thymus brachychilus jalas: A rich source of ursolic and oleanolic acids(Taylor & Francis Inc, 2023) Akdeniz, Mehmet; Yiğitkan, Serkan; Yılmaz, Mustafa Abdullah; Yener, İsmail; Varhan Oral, Elif; Fırat, Mehmet; Orhan, İlkay ErdoğanThe significance of Thymus species in the scientific community is growing steadily due to their extensive utilization in traditional medicine, food industry, and pharmaceutical sector, owing to their abundance in essential oil and phytochemical content, rendering them commercially significant species. The current work focuses on conducting a comprehensive analysis of the ethanol extract and essential oil derived from the root and aerial portions of Thymus brachychilus Jalas, an endemic species that has not been previously investigated. Additionally, a novel GC-MS (gas chromatography-mass spectrometry) technique was developed to quantify the levels of triterpenoids, which are frequently found in many plants, particularly those belonging to the Lamiaceae family. The approach was then used to assess the triterpenoid content of the species. While the phenolic content of the species was determined by LC-MS/MS (liquid chromatography-tandem mass spectrometry), the chemical composition of triterpenoid, essential oil and flavor (aroma) of the plant was determined by GC-MS. Eucalyptol was the primary ingredient in both the essential oil and the flavor, accounting for 11.05% and 12.35%, respectively. In the DPPH and ABTS radical scavenging techniques, the root ethanol extract exhibited the greatest antioxidant activity with IC50 values of 26.70 +/- 0.23 and 19.16 +/- 0.11 mu g/mL, respectively. There was a high level of urease (59.54 +/- 1.67% at 100 mu g/mL) inhibitory activity observed in the root ethanol extract in enzyme inhibition assays, as well as angiotensin (94.80 +/- 0.56%), elastase (40.19 +/- 0.39%), and collagenase (48.26 +/- 0.12%) inhibition in the aerial ethanol extract. Moreover, the MCF-7 cell line for breast cancer had a strong cytotoxic impact when exposed to the essential oil of the species (vitality%: 1.45 +/- 0.01 at 200 mu g/mL. The LC-MS/MS and GC-MS studies revealed that the roots had a significant concentration of rosmarinic acid (15,801 mu g analyte/g extract), but the aerial portions had a substantial quantity of ursolic acid (152,374 mu g analyte/g extract). The plant exhibits potential in the food, cosmetic, and pharmaceutical sectors on account of its elevated levels of rosmarinic and ursolic acid, superior antioxidant capacity, and angiotensin and urease inhibitory effect.Öğe Content analysis, nanoparticle forming potential and acaricidal effects on Tick (Hyalomma marginatum) of essential oils from two common Thymbra species(Taylor & Francis Ltd, 2024) Kaya, Eda Cavus; Akdeniz, Mehmet; Ugurlu, Pelin; Yener, Ismail; Firat, Mehmet; Ertas, AbdulselamIn this study, the fatty acid, essential oil and aroma composition of Thymbra sintenisii Bornm. & Aznav. subsp. sintenisii Bornm. & Aznav (TSS) and Thymbra sintenisii Bornm. & Azn. subsp. isaurica P.H.Davis (TSI) were determined by GC-MS. The cytotoxic activity was determined with MTT assay and antioxidant activities were determined with DPPH, ABTS, CUPRAC methods. Urease, acetyl, butyryl, angiotensin, alpha-amylase, tyrosinase, elastase, and collagenase enzyme inhibition activities of these species were also determined. The acaricidal effects of essential oils from aerial parts of TSS and TSI on ticks and the nanoparticle formation potential of their essential oils with AgNO3 were determined and compared. The main components of TSS essential oil and aroma were determined as carvacrol and o-cemen (66.78 and 35.44%, respectively), the major components of TSI essential oil and aroma were carvacrol and gamma-terpinene (63.78 and 31.44%, respectively). The species exhibited remarkably high antioxidant activity, particularly in the ABTS and CUPRAC methods. It was also determined that the butyrylcholinesterase and acetylcholinesterase enzyme inhibition activities (Inhibition%: 76.12 +/- 0.83 and 74.13 +/- 0.33, respectively) of the species were quite high. Moderate antimicrobial activity against the bacteria was observed for both essential oils, with MIC values ranging from 312 to 1250 mu g/mL. Moreover, essential oils of these species showed good acaricidal activity on Hyalomma marginatum (LC50: 11.82 mu g/mL for TSS; LC50: 57.64 mu g/mL for TSI). The high antioxidant, anticholinesterase, and acaracidal activities observed in both species are attributed to the presence of carvacrol in their essential oils. The species have potential to be used in food supplements-preservatives, pharmaceutical industry.Öğe Cream production and biological in vivo/in vitro activity assessment of a novel boron-based compound derived from quercetin and phenyl boronic acid(Elsevier GmbH, 2022) Temel, Hamdi; Atlan, Metin; Ertaş, Abdulselam; Yener, İsmail; Akdeniz, Mehmet; Yazan, Zehra; Yılmaz, Mustafa AbdullahBoronic acids constitute an important class of synthetic intermediates due to their high chemical stability, ease of use, moderate organic Lewis acid properties, reduced reactivity profiles and numerous biological activities such as antibacterial and antioxidant. The present study documents the synthesis and characterization of a novel boronic ester compound (3,5,7-trihydroxy-2- (2-phenyl benzo [d] [1,3,2] dioxaborol-5-yl) −4H-chromen-4-a) which was derived from phenyl boronic acid and quercetin. The new boron-based compound was used in the cream formulation after evaluating its antioxidant, antibacterial, anti-enzyme, anticancer activities and electrochemical oxidation behaviour. Furthermore, the cream has been dermatologically and microbiologically tested. Also, histological evaluation of the agent was estimated on multiple rat organs by hematoxylin-eosin staining method. Antioxidant potential of the new compound was tested by ABTS cation radical (IC50: 0.11 ± 0.01 µg/mL), DPPH free radical scavenging (IC50: 0.14 ± 0.01 µg/mL), and CUPRAC (A0.5: 1.73 ± 0.16 µg/mL) methods, respectively. The compound determined to have a dominant antioxidant activity. In addition, the synthesized compound had no toxic effect on the healthy cell line (PDF), while having a very high (IC50: 18.76 ± 0.62 µg/mL) cytotoxic effect on the cancerous cell line (MCF-7). In general, the compound showed moderate acetylcholinesterase enzyme activity (IC50: 115.63 ± 1.16 µg/mL), high butyrylcholinesterase (IC50: 3.12 ± 0.04 µg/mL), antiurease (IC50: 1.10 ± 0.06 µg/mL), and antithyrosinase (IC50: 11.52 ± 0.46 µg/mL) enzyme activities. In addition, the compound was found to be effective against Escherichia coli (ATCC 25922) bacteria studied at concentrations of 6.50 mg/mL. Moreover, the test results of the boronic ester compound used in the cream formulation demonstrated that it was microbiologically and dermatologically appropriate. Histologic analysis showed that the control group and experimental group were at similar properties without significant change. The phenyl boronic acid derivative compound synthesized from quercetin may have higher biological activity potential than quercetin. Due to the high biological activity potential of the synthesized compound, it has the potential to be used in food, feed, pharmaceutical and cosmetic industries.Öğe A detailed biological and chemical investigation of sixteen schillea species' essential oils via chemometric approach(Wiley-V C H Verlag Gmbh, 2020) Yener, İsmail; Yılmaz, Mustafa Abdullah; Ölmez, Özge Tokul; Akdeniz, Mehmet; Tekin, Fetullah; Haşimi, Nesrin; Alkan, Mehmet Hüseyin; Öztürk, Mehmet; Ertaş, Abdülselam; 0000-0002-2093-4949; 0000-0001-8932-4535; 0000-0002-0988-9462; 0000-0002-4090-7227; 0000-0002-0761-6436; 0000-0002-2193-8386Representatives of the Achillea genus are widely used as foods or nutraceuticals. Considering the increasing demand for herbal dietary supplements with health promoting effects, the objective of this research was to evaluate the chemical composition and biological activities of the essential oils obtained from sixteen Achillea species (A. biebersteinii, A. wilhelmsii subsp. wilhelmsii, A. aleppica subsp. zederbaueri, A. vermicularis, A. monocephala, A. nobilis, A. goniocephala, A. sintenisii, A. coarctata, A. kotschyi subsp. kotschyi, A. millefolium subsp. millefolium, A. lycaonica, A. spinulifolia, A. teretifolia, A. setacea, and A. schischkinii). Anticholinesterase, antiurease, antityrosinase enzymes inhibition, antioxidant, antimicrobial, toxic and cytotoxic activities of obtained essential oils were investigated. DPPH activities were found to be very low in all studied samples, while ABTS and CUPRAC antioxidant activities were found to be moderate. In addition, all samples were found to have moderate anticholinesterase and antimicrobial effects. It has been determined that the studied species have low cytotoxicity and high toxicity. Besides, chemical composition of the essential oils were determined by GC/MS and the results were chemometrically analyzed. The chemometric analyses of Achillea species collected from nine different regions were accomplished by principal component analysis (PCA) and hierarchical cluster analysis (HCA) techniques. According to the PCA analysis, A. nobilis subsp. neilreichii was found to be different from all studied species in terms of essential oil composition. The major components found in these species were piperitone, camphor, alpha-terpinene, eucalyptol, artemisia ketone, endo-borneol, beta-eudesmol and verbenol. The fact that camphor was toxic and found in majority of the studied species stands out as a remarkable result.Öğe The Detailed Chemical and Biological Analysis of Ziziphora clinopodioides Lam. Species Growing in Cultural and Natural Environments(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2024) Cavusoglu, Mehmet; Yigitkan, Serkan; Yener, Ismail; Caglayan, Mehmet Veysi; Resitoglu, Baris; Akdeniz, Mehmet; Kaya, Eda C. A. V. U. S.The Lamiaceae family is an important resource for the pharmaceutical, food, cosmetic, and perfumery sectors. The species Ziziphora clinopodioides Lam., belonging to the Lamiaceae family, has been used in folk medicine since ancient times. The aim of the present study was to determine the total phenolic and flavonoid contents, antioxidant, cytotoxic and enzyme (AChE, BChE, tyrosinase, urease, elastase, collagenase and ACE) inhibition activities of ethanol extracts of the aerial parts of natural and cultivated samples of the species Z. clinopodioides. In addition, the aroma contents of the species were determined by GC-MS/FID, and the phytochemical composition of the ethanol extracts was determined by LC-MS/MS. According to the aroma analysis results, the major components of the culture sample of the Z. clinopodioides species were determined as pulegone (39.83%), cis- menthone (21.36%), trans-menthone (16.64%), while the major components of the natural sample were determined as pulegone (62.42%), neoisomenthol (5.93%) and cis-pulegone oxide (5.47%). According to LC-MS/MS results, quinic acid (25.841, 15.694 mg analyte g-1 extract, respectively), rosmarinic acid (6.804, 25.523), and acacetin (6.115, 10.764) were detected as major compounds in cultured and natural samples of the species. Also, hesperidin (5.725) was higher in culture sample. According to the enzyme activity results, it was determined that cultured and natural samples of the species showed moderate butyrylcholinesterase enzyme inhibition activity (inhibition %: 45.14 +/- 1.40; 43.57 +/- 0.73, respectively). In urease enzyme inhibition activity, it was determined that culture sample showed moderate activity (inhibition %: 43.64 +/- 0.39) but natural sample did not show activity, and culture sample showed high antihypertensive activity (inhibition %: 81.6 +/- 1.19). When the results were evaluated in general, it was determined that the cultured sample of the species showed higher antioxidant, butyrylcholinesterase, urease, and antihypertensive activity compared to natural sample.Öğe Determination of enzyme inhibition potential and anticancer effects of pistacia khinjuk stocks raised in in vitro and in vivo conditions(MDPI, 2021) Tilkat, Emine Ayaz; Batıbay, Hayri; Yener, İsmail; Yılmaz, Pelin Köseoğlu; Akdeniz, Mehmet; Kaplan, Alevcan; Ercişli, Sezai; Ertas, Abdulselam; Holubec, VojtechIn this study, antihypertensive, anticholinesterase, antiurease, antityrosinase and antielastase enzyme inhibition and anticancer activities of in vivo (male and female) and in vitro samples (root, stem and leaf parts) of the Pistacia khinjuk Stocks were investigated comparatively. In this context, in vitro shoot cultures were obtained from germinated mature seeds. Then, the juvenile shoots were proliferated in Murashige and Skoog (MS) medium supplemented with 1 mg/L 6-Benzylaminopurine (BAP). In terms of anticancer activity, the whole of the samples studied was found to have apoptotic effects against MCF-7 (breast cancer) and HT-29 (colon cancer) cell lines. The extracts obtained from in vivo female root parts showed better cytotoxicity than all the other tested extracts on MCF-7 (IC50: 31.86 +/- 1.40 mu g/mL) and HT-29 cell series (IC50: 59.60 +/- 0.69 mu g/mL). Even though all the samples showed a strong butyrylcholinesterase enzyme inhibition (BChE) activity, it was detected that none of the samples had shown acetylcholinesterase enzyme inhibition (AChE). It was also determined that in vivo leaf samples of female trees had the highest BChE activity (Inhibition%: 75.20 +/- 1.50). All the samples showed a low-moderate level of urease and tyrosinase enzyme activity, while in vivo samples showed a significant level of the elastase enzyme activities (Inhibition%: 58.72 for female root extracts; 58.25 for female leaf extracts, at 50 mu g/mL concentration), and they were more active than the standard oleanolic acid (Inhibition%: 39.46 +/- 0.52). The antihypertensive activities as the inhibition of angiotensin I-converting enzyme (ACE) of in vivo samples (Inhibition%: 95.88 for female stem extracts; 95.18 for female root extracts) were detected as close to the standard (Inhibition%: 96.64 +/- 1.85) used. In general, it can be stated that in vivo samples had higher biological activities compared to in vitro ones. Consequently, according to our results, it was concluded that in vitro stem parts of khinjuk pistachio could also be evaluated as an alternative new antihypertensive, antielastase and anticancer agent source.Öğe Determination of essential oil and biological activities of Hypericum ternatum Poulter and H. scabrum L. species collected from different localities: is H. scabrum an alternative to multifunctional species ST JOHN'S WORT (H. perforatum)?(Tubitak Scientific & Technological Research Council Turkey, 2022) Akdeniz, Mehmet; Yener, Ismail; Irtegun Kandemir, Sevgi; Ozhan Kocakaya, Safak; Firat, Mehmet; Yigitkan, Serkan; Hasimi, NesrinThe importance of Hypericum L. species, being used in traditional medicine, in the scientific world is increasing day by day. Hypericum species are plants that have been used in the treatment of many diseases for a long time and have healing properties. In the current study, the essential oil compositions of Hypericum scabrum L. and H. ternatum Poulter collected from different localities in Turkey were determined by GC-MS/FID. In addition, their antioxidant, cytotoxic, and antimicrobial activities with their enzyme inhibitory potentials (cholinesterase, urease, tyrosinase, elastase, and collagenase) were investigated. Also, in vitro and in silico studies of the major components of the species have been carried out on the studied enzymes. It was determined that four H. scabrum samples mainly contained alpha-pinene (55.99%-62.80%) while three H. ternatum samples contained 2-methyloctane (9.45%-22.39%) and alpha-pinene (12.75%-33.08%). While H. scabrum essential oils possessed significant biological activity potential except for the antimicrobial activity, H. ternatum samples did not. All essential oil samples of H. scabrum exhibited a high cytotoxic effect (IC50 21.67 +/- 0.34 to 34.67 +/- 0 .45 mu g/mL) against colon cancer cell line (HT-29) and indicated higher acetyl-(Inhibiton% 83.30 +/- 1.90 to 93.08 +/- 1.04) and butyryl-cholinesterase (Inhibiton% 80.58 +/- 1.19 to 93.28 +/- 1.99) inhibitory activity than the standard compound, galantamine. Furthermore, all samples of H. scabrum showed high tyrosinase (Inhibiton% 69.00 +/- 1.64 to 95.25 +/- 1.42) and elastase (Inhibiton% 27.58 +/- 0.34 to 36.69 +/- 0.18) inhibitory effects. These biological activity results indicated that H. scabrum essential oil could be used in the cosmetic and pharmaceutical industries.Öğe Determination of fingerprints contents of different extracts and parts of six endemic Salvia taxa by GC-MS: Source species for valuable compounds with drug or drug potential(Wiley, 2022) Akdeniz, Mehmet; Yener, İsmail; Dinçel, Demet; Fırat, Mehmet; Değirmenci, Dilek Karataş; Ertaş, AbdulselamPublic use of Salvia species and their importance in the scientific world is continually increasing. It is known that this use and the importance of Salvia species are mostly due to the terpenoid compounds that they contain. In this context, the terpenoid-steroid-flavonoid contents of extracts of six endemic Salvia (S. kurdica, S. pseudeuphratica, S. rosifolia, S. siirtica, S. cerino-pruinosa var. cerino-pruinosa and S. cerino-pruinosa var. elazigensis) species prepared with different solvents were determined by gas chromatography-mass spectrometry. Within the framework of the ingredient analysis, content analysis of the ethanol extracts of the root, branch, leaf and flower parts of the species collected in the same period between 2015 and 2017 years was performed. In general, extracts prepared with chloroform and ethanol were found to contain a wide variety of compounds while petroleum ether extracts were found to contain much less varied compounds. In addition, in general, root extracts are richer in terpenoid compounds than aerial part extracts. Some species can be used as source species in terms of ferruginol, cryptanol, 6,7-dehydroroyleanone, lup-(20)29-ene-2 alpha-hydroxy-3 beta-acetate, salvigenin and beta-sitosterol contents (52,114.28, 75,979.08, 101,247.41, 40,071.29, 33,952.13 and 34,010.90 mu g analyte/g extract, respectively).Öğe Essential oil content, in-vitro and in-silico activities of Hypericum triquetrifolium Turra, H. empetrifolium subsp. empetrifolium Willd., and H. pruinatum Boiss. & Balansa species(Istanbul Univ, Fac Pharmacy, 2023) Akdeniz, Mehmet; Yener, Ismail; Kocakaya, Safak Ozhan; Yolcu, Murat; Yigitkan, Serkan; Aydin, Firat; Turkmenoglu, Fatma PinarBackground and Aims: The importance of Hypericum species that are used traditionally against many diseases is increasing day by day.Methods: In this study, the essential oil contents of Hypericum triquetrifolium, H. empetrifolium subsp. empetrifolium, and H. pruinatum species were determined with GC-MS/ FID. This is the first study on the antioxidant, anticholinesterase, anti-urease, antityrosinase, antielastase, and anticollagenase activities of these species. Also, in silico and in vitro enzyme in-hibitory activities of the major compounds in the essential oil samples of the species have been evaluated. In addition, the cytotoxic effects of the essential oils were determined by the MTT method.Results: According to GC-MS/ FID results, the major compounds were determined as caryophyllene oxide (16.76%) for H. triquetrifolium, alpha-pinene (21.67%) for H. empetrifolium subsp. empetrifolium, and germacrene D (22.47%) for H. pruinatum. Especially, H. pruinatum sample showed a high cytotoxic effect (IC50: 34.78 +/- 0.22 and 29.06 +/- 0.40 mu g/mL, respectively) on HT -29 and MCF-7 cell lines. It was determined that the same sample showed a promising inhibitory activity on acetyl (18.33 +/- 2.79, 36.48 +/- 2.40, and 56.97 +/- 0.94, respectively) and butyryl (71.63 +/- 2.78, 73.88 +/- 1.16, and 56.97 +/- 0.97, respectively) cholinesterase enzymes.Conclusion: Results of the in-vitro activity studies indicated that H. pruinatum essential oil could be used in the pharmaceuti-cal industry.Öğe Essential oil contents of Hypericum linarioides , H. helianthemoides, and H. lydium with their biological activities: Importance of Hypericum genus in the cosmeceutical and pharmaceutical industries(Springer, 2023) Akdeniz, Mehmet; Yener, İsmail; Ertaş, Abdülselam; Dincel, Demet; Fırat, Mehmet; Kocakaya, Şafak Özhan; Yigitkan, Serkan; Türkmenoğlu, Fatma Pınar; Aydın, Fırat; Kolak, UfukIn the literature, more than 3000 studies have been carried out on species in the Hypericum genus in the last ten years, especially for H. perforatum. The importance of Hypericum species in traditional medicine and modern medicine is increasing day by day. From this point of view, the chemical composition of H. linarioides, H. helianthemoides, and H. lydium essential oils was analyzed by GC-MS/FID. In addition, their antioxidant, cytotoxic and antimicrobial activities along with their enzyme inhibitory potentials (cholinesterase, urease, tyrosinase, elastase and, collagenase) were defined. Also, in vitro and in silico studies of the major components of the species have been carried out on the studied enzymes. H. linarioides and H. helianthemoides were found to be rich in monoterpene hydrocarbons (63.96% and 43.86%, respectively), and H. lydium in sesquiterpene hydrocarbons (48.41%). The major components were indicated as α-pinene for H. linarioides (50.39%) and for H. helianthemoides (36.84%), and β-selinene for H. lydium (10.12%). H. lydium essential oil exhibited very high cytotoxic activity on breast cancer (MCF-7) (selectivity indices SI: 12) and colon cancer (HT-29) (SI: 2) cell lines. In particular, the selectivity indice value in MCF-7 cell lines of H. lydium could have natural therapeutic potential for breast cancer. H. lydium possessed neuropharmacological potential due to its high anticholinesterase activity. Since H. linarioides indicated high anti-aging potential (tyrosinase, elastase, and collagenase enzyme inhibitory activities), it could be used in the cosmetic industry.Öğe Essential oil, aroma, and fatty acid profiles of five endemic Salvia taxa from Turkey with chemometric analysis(Wiley-V C H Verlag GMBH, 2022) Ertaş, Abdulselam; Akdeniz, Mehmet; Yener, İsmail; Öztürk, Mehmet; Ölmez, Özge Tokul; Fırat, Mehmet; Kolak, UfukThe essential oil, aroma, and fatty acid compositions of Salvia cerino-pruinosa var. cerino-pruinosa, S. cerino-pruinosa var. elazigensis, S. pseudeuphratica, S. rosifolia, and S. kurdica collected in 2015, 2016, and 2017 were analysed by GC-FID, GC/MS/Head Space and GC/MS. The results were evaluated chemometrically for principal component analyses and hierarchical clustering analyses using Minitab program. The main components of their essential oils have been determined as germacrene-D (41.79 % highest for these species), camphor (55.80 %), linalool (33.38 %), caryophyllene oxide (32.99 %), 1,8-cineole (26.30 %) and geraniol acetate (31.63 %), while for aroma 1,8-cineole (45.95 % highest for these species), camphor (58.54 %), D-limonene (40.83 %), linalool (27.67 %) and cis-linalool oxide (25.87 %). Oleic (72.65 % highest for these species), erucic (44.61 %), 2-palmitoleic (42.17 %) and nervonic (32.61 %) acids were determined as major components of their fatty acids. According to the PCA and HCA, the essential oil components of these Salvia taxa unaffected by the years, and accordingly, the Salvia taxa grouped among themselves.
