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    Acinetobacter mesopotamicussp. nov., Petroleum-degrading Bacterium, Isolated from Petroleum-Contaminated Soil in Diyarbakir, in the Southeast of Turkey
    (Springer, 2020) Acer, Omer; Guven, Kemal; Poli, Annarita; Di Donato, Paola; Leone, Luigi; Buono, Lorena; Guven, Reyhan Gul
    A new petroleum-degrading bacterium, designated strain GC2(T), was isolated from Bozkus 1 petroleum station in Diyarbakir, located in the southeast of Turkey. Cells were Gram-negative staining, aerobic, coccoid-rods, non-motile, non-spore-forming. The bacterium was found to degrade 100% ofn-alkanes ranging from C11 to C34 presented in the 1% crude oil after incubation of 7 days. The membrane phospholipids were 1,2 diacylglycero-3-phosphorylethanolamine (PEA), phosphatidylglycerol (PG), dipalmitoyl-sn-glycerol 1- phosphocholine (PC1), 1,2 dipalmitoyl-sn-glycero-3-phosphocholine monohydrate (PC3), cardiolipin also called diphosphatidylglycerol (CL) and l-alpha- phosphatidic acid, dipalmitoyl (AP); predominant respiratory ubiquinone was Q-8 and C16:0, C18:1 omega 9c and C16:1 were the major cellular fatty acids. The 16S rRNA sequence analysis revealed that the strain GC2(T)was a member of genusAcinetobacterand was most closely related toAcinetobacter lwoffiiDSM 2403( T)(99.79%),Acinetobacter pseudolwoffiiANC 5318( T)(98.83%) andAcinetobacter harbinensisHITLi 7( T)(98.14%). TherpoBandgyrBgene sequence analysis confirmed that the strain GC2(T)was a member of genusAcinetobacterand that the closest relative wasAcinetobacter lwoffiiDSM 2403( T)(99.08% and 100% similarity, respectively). DNA-DNA hybridization values between GC2(T)and its closest relatives ranged from 65.6% (withA. lwoffii) to 5.1% (withA. venetianus). The whole genome sequence of strain GC2(T)was obtained. The DNA G + C content of this strain was determined to be 42.9 mol %. ANI indexes, in silico estimations of DDH values and wet lab DDH values demonstrated that strain GC2(T)represents an independent genomospecies. On the basis of phenotypic characteristics, chemotaxonomic, phylogenetic data and DNA-DNA hybridization and whole genome analysis, we propose to assign strain GC2(T)as a new species of the genusAcinetobacter, for which the nameAcinetobacter mesopotamicussp. nov. is proposed. The type strain of this species is GC2(T)(DSM 26953 T = JCM 31073 T). The whole genome of strain GC2(T)has been deposited at DDBJ/ENA/GenBank under the accession JAALFF010000000.
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    Anoxybacillus sp AH1, an ?-amylase-producing thermophilic bacterium isolated from Dargecit hot spring
    (Springer, 2015) Acer, Omer; Pirinccioglu, Hemse; Bekler, Fatma Matpan; Gul-Guven, Reyhan; Guven, Kemal
    The present study was conducted to isolate alpha-amylase-producing thermophilic bacteria from Dargecit hot springs in Turkey. The morphological, biochemical and physiological characterisation, as well as genetic analysis by 16S rRNA sequences indicated that the isolated strain AH1 was a member of Anoxybacillus genus. The strain was aerobe, Gram-positive and spore-forming rod, exhibiting optimum growth temperature and pH of 60 degrees C and 7.0-7.5, respectively. Optimization of growth medium and enzyme assay conditions for extracellular a-amylase production by the novel thermophilic Anoxybacillus sp. AH1 were carried out in many different media containing a variety of carbon and nitrogen sources. Among various carbon and nitrogen sources, peptone (2054.1 U/mL) at 1% and maltose (1862.9 U/mL) at 0.5% increased a-amylase activity, compared to controls. Moreover, a high enzyme production was observed with potato starch at 0.5% and 1% (2668.4 U/mL and 3627 U/mL, respectively), as well as with 1% soluble starch (2051.9 U/mL). The enzyme activity was found to be rather high in the presence of CaCl2 up to 100 mM.
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    Bioaccumulation, tolerance, and removal of U(VI) and Th(IV) by a novel thermophilic Bacillus cereus ST14 isolated from hot spring mud samples in Afyonkarahisar, Turkey
    (Springer Heidelberg, 2024) Ozdemir, Sadin; Acer, Omer; Kilinc, Ersin
    In this study, a novel thermophilic Bacillus cereus ST14 with heavy metal tolerance was isolated from a hot spring mud sample in Afyonkarahisar (Omer). 16S rRNA analysis revealed that strain ST14 was mostly related to Bacillus cereus strain 151,007-R3_K09_40_27F (98.18%) and Bacillus cereus strain 2 k (98.10%). We hypothesized that U(VI) and Th(IV) affected B. cereus at the cell level and that this bacterium can be used as a bioindicator. Therefore, U(VI) and Th(IV) tolerance, removal, bioaccumulation, and impacts on antioxidant enzymes of thermophilic B. cereus have been examined in detail. Changes in the surface macrostructure and functionality of B. cereus following interaction with U(VI) and Th(IV) were studied using a scanning electron microscope and fourier transform infrared spectroscopy. B. cereus was found to be highly resistant to 10 and 12.5 mg/L of U(VI) and Th(IV) after 24-h incubation. Superoxide dismutase (SOD) and catalase (CAT) activities were observed to be increased by different U(VI) and Th(IV) concentrations. The highest bioaccumulation capacity quantities were detected as 100.79 and 87.96 mg metal/g dried bacteria at 24th h at 12.5 mg/L concentration for U(VI) and Th(IV), respectively. The highest U(VI) and Th(IV) cell membrane bioaccumulation capabilities of B. cereus have been measured as 318.94 and 263.89 mg metal/g wet membrane, respectively, at 24 h and 12.5 mg/L. This study's findings suggested that B. cereus could be employed as a bioindicator organism for detecting metal contamination in natural or industrial water samples. However, further research should focus on other thermophilic microorganisms.
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    Characterisation of a partially purified protease from Bacillus cereus KG5 isolated from a hot spring
    (Elsevier Sci Ltd, 2011) Ahmetoglu, Nazenin; Bekler, Fatma Matpan; Guven, Reyhan Gul; Acer, Omer; Guven, Kemal
    [Abstract Not Available]
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    Combined effects of some chemicals on ?-galactosidase activity using a new semiparametric errors in variables model
    (Banats Univ Agricultural Sciences & Veterinary Medicine Timisoara, 2019) Yalaz, Secil; Matpan Bekler, Fatma; Acer, Omer
    The combined effects of some chemicals on beta-galactosidase activity by Anoxybacillus sp. FMB1 were studied using semiparametric errors in variables methodology when the error has an unknown distribution. An experimental design was chosen to explain six chemicals, dithiothreitol (DTT), phenylmethylsulfonyl fluoride (PMSF), N-ethylmaleimide (NEM), iodoacetamide (lod A), beta-Mercaptoethanol (beta-Mer) and 1,10-phenanthroline (phen) and to investigate the results. This technique constrained the number of real experiments performed while considering conceivable interactions between six chemicals. The optimal combinations of chemical concentration for maximum beta-galactosidase activity was determined as 8 mM DTT, 8 mM PMSF, 8 mM NEM, 8 mM lod A, 2 mM beta-Mer and 2 mM phen. We also compared the no measurement error case and errors in variables case. The proposed estimator shown has better performances.
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    Isolation and characterization of long-chain alkane-degrading Acinetobacter sp BT1A from oil-contaminated soil in Diyarbakir, in the Southeast of Turkey
    (Taylor & Francis Inc, 2016) Acer, Omer; Guven, Kemal; Bekler, Fatma Matpan; Gul-Guven, Reyhan
    A strain of long-chain alkane-degrading bacteria, BT1A, was isolated from oil-contaminated soil in Diyarbakr, in the southeast of Turkey. Morphological, biochemical, and physiological characterization and 16S rRNA gene sequence analysis showed that the strain BT1A was a member of Acinetobacter genus, and it was found to be closely related to Acinetobacter baumannii. The strain BT1A was able to utilize crude petroleum as carbon and energy sources in order to grow. Among the aliphatic hydrocarbons, growth was observed only in the medium containing long-chain alkanes (tridecane, pentadecane, and hexadecane) and squalene. Hexadecane was the most preferred hydrocarbon among the long-chain alkanes. Gas chromatography-mass spectrometry (GC-MS) analysis showed that BT1A degraded 83% of n-alkanes of 1% crude oil in 7days. The present study indicates that the isolated strain can well be used for biodegradation of hydrocarbons in oil-contaminated sites.
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    Isolation and characterization of long-chain alkane-degrading Acinetobacter sp. BT1A from oil-contaminated soil in Diyarbakir, in the Southeast of Turkey (vol 20, pg 80, 2016)
    (Taylor & Francis Inc, 2017) Acer, Omer; Guven, Kemal; Bekler, Fatma Matpan; Gul-Guven, Reyhan
    [Abstract Not Available]
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    Isolation and identification of petroleum degrading bacteria
    (Elsevier Sci Ltd, 2011) Guven, Reyhan Gul; Bekler, Fatma Matpan; Kaya, Hasan; Acer, Omer; Guven, Kemal; Temel, Hamdi
    [Abstract Not Available]
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    PURIFICATION OF THERMOSTABLE ?-GALACTOSIDASE FROM ANOXYBACILLUS SP KP1 AND ESTIMATION OF COMBINED EFFECT OF SOME CHEMICALS ON ENZYME ACTIVITY USING SEMIPARAMETRIC ERRORS IN VARIABLES MODEL
    (Parlar Scientific Publications (P S P), 2017) Bekler, Fatma Matpan; Yalaz, Secil; Acer, Omer; Guven, Kemal
    The thermostable beta-galactosidase from thermophilic Anoxybacillus sp. KP1 was partially purified by Sephadex G-75 gel permeation chromatography with a fold purification of 14.4 and a yield of 11.8%, respectively. The optimal activity of purified beta-galactosidase was pH 9.0 and a temperature of 60 degrees C. The enzyme was stable at pH 9.0. Thermostability of the enzyme was also 60 degrees C. The molecular weight of the enzyme was determined as 68 kDa by SDS-PAGE. The partially purified thermostable alkaline beta-galactosidase was significantly inhibited by various divalent cations, including Hg2+ and Cu2+ while Ca2+ and Mg2+ ions activated the enzyme activity. The combined effects of some chemicals on beta-galactosidase activity by Anoxybacillus sp. KP1 were studied using semiparametric errors in variables methodology. The optimal combinations of chemicals concen-tration for maximum beta-galactosidase activity was determined as 4 mM DTT, 4 mM PMSF, 4 mM NEM, 4 mM Iod A, 4 mM beta-Mer and 10 mM phen.
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    Simultaneous preconcentrations of Cu(II), Ni(II), and Pb(II) by SPE using E. profundum loaded onto Amberlite XAD-4
    (Elsevier, 2021) Ozdemir, Sadin; Kilinc, Ersin; Acer, Omer; Soylak, Mustafa
    In the present study, we used E. profundum loaded onto Amberlite XAD-4 resin for solid-phase extraction (SPE) of Cu(II), Ni(II), and Pb(II). Some conditions of the SPE procedure, for instance, sample flow rate, pH, eluent type and concentration, the sample volume, amount of E. profundum XAD-4 resin, and the effects of foreign in-terferences ions are discussed in detail. Surface microstructures of the biosorbent were characterized by FTIR, FE-SEM/EDX before and after loading of metals. The biosorption capacities of E. profundum were determined as 49.83, 57.12, and 64.35 mg g(-1) for Pb(II), Cu(II), and Ni(II), respectively. The LOD values were calculated as 0.031 ng mL(-1), 0.042 ng mL(-1), and 0.043 ng mL(-1) for Cu(II), Ni(II), and Pb(II), respectively. The linear range was detected as 0.25-25 ng mL(-1). RSD values were lower than 5%. The concentrations of metal ions in water and food samples were measured by ICP-OES.