Combined effects of some chemicals on ?-galactosidase activity using a new semiparametric errors in variables model
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Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Banats Univ Agricultural Sciences & Veterinary Medicine Timisoara
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The combined effects of some chemicals on beta-galactosidase activity by Anoxybacillus sp. FMB1 were studied using semiparametric errors in variables methodology when the error has an unknown distribution. An experimental design was chosen to explain six chemicals, dithiothreitol (DTT), phenylmethylsulfonyl fluoride (PMSF), N-ethylmaleimide (NEM), iodoacetamide (lod A), beta-Mercaptoethanol (beta-Mer) and 1,10-phenanthroline (phen) and to investigate the results. This technique constrained the number of real experiments performed while considering conceivable interactions between six chemicals. The optimal combinations of chemical concentration for maximum beta-galactosidase activity was determined as 8 mM DTT, 8 mM PMSF, 8 mM NEM, 8 mM lod A, 2 mM beta-Mer and 2 mM phen. We also compared the no measurement error case and errors in variables case. The proposed estimator shown has better performances.
Açıklama
Anahtar Kelimeler
Beta-Galactosidase, Inhibition, Semiparametric Eiv Model, Unknown Distribution
Kaynak
Banats Journal of Biotechnology
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
10
Sayı
19
