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    Anticancer Activities of Zinc Oxide Nanoparticles (ZnONPs) Synthesized from Mentha longifolia L. Leaf Extract
    (2024) İpek, Polat; Baran, Mehmet Fırat; Hatipoğlu, Abdulkerim; Baran, Ayşe
    Recently, there has been a remarkable increase in cancer and cancer-related deaths. In this study, the impacts of zinc oxide nanoparticles (ZnONPs) produced from the aqueous leaf extract of Mentha longifolia L. (ML) on ovary adenocarcinoma (OVCAR-3), colorectal carcinoma (HCT-116), and healthy retinal pigment epithelial cell (RPE-1) lines were investigated. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test was performed to discover its antiproliferative properties. As a result of the application of ML-ZnONPs on RPE-1, OVCAR-3, and HCT-116 cell lines at doses (µg/mL) of 250, 500, and 1000 for 24 hours, the viability rates (%) in the cell lines were 18.73-30.56, 21.98-28.76, and 27.27-40.93, respectively. In the 48-hour application, the viability rates (%) of the same cells were between 29.51-46.83, 32.49-40.81, and 46.82-44.37, respectively. The MTT test revealed that ML-ZnONPs strongly suppressed the growth of RPE-1, OVCAR-3, and HCT-116 cell lines. The test showed that the effect of dose increase and time on the viability of both cancer cell lines was negative.
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    Anticancer Activities of Zinc Oxide Nanoparticles (ZnONPs) Synthesized from Mentha longifolia L. Leaf Extract
    (Iğdır Üniversitesi, 2024) İpek, Polat; Baran, Mehmet Fırat; Hatipoğlu, Abdulkerim; Baran, Ayşe
    Recently, there has been a remarkable increase in cancer and cancer-related deaths. In this study, the impacts of zinc oxide nanoparticles (ZnONPs) produced from the aqueous leaf extract of Mentha longifolia L. (ML) on ovary adenocarcinoma (OVCAR-3), colorectal carcinoma (HCT-116), and healthy retinal pigment epithelial cell (RPE-1) lines were investigated. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test was performed to discover its antiproliferative properties. As a result of the application of ML-ZnONPs on RPE-1, OVCAR-3, and HCT-116 cell lines at doses (µg/mL) of 250, 500, and 1000 for 24 hours, the viability rates (%) in the cell lines were 18.73-30.56, 21.98-28.76, and 27.27-40.93, respectively. In the 48-hour application, the viability rates (%) of the same cells were between 29.51-46.83, 32.49-40.81, and 46.82-44.37, respectively. The MTT test revealed that ML-ZnONPs strongly suppressed the growth of RPE-1, OVCAR-3, and HCT-116 cell lines. The test showed that the effect of dose increase and time on the viability of both cancer cell lines was negative.
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    Antioxidant properties of allium turcicum Özhatay & cowley plant extract, its effects on the proliferation and migration of cancer cells
    (Frontiers Media SA, 2024) İpek, Polat; Baran, Ayşe; Cebe, Deniz Barış; Ahmadian, Elham; Eftekhari, Aziz; Baran, Mehmet Fırat
    Cancer is a type of non-communicable disease that is responsible for numerous deaths worldwide. Cancer incidence and mortality rates are on the rise due to a combination of factors, such as a growing population, aging, and poor dietary habits. The Allium turcicum Özhatay & Cowley plant is an endemic plant in the area where it grows and is consumed by the public due to its various benefits. This endemic plant, which generally grows in high-altitude regions, is sold in bunches because it is costly, mixed with rock salt, crushed into powder, and consumed as a spice. The cytotoxic and growth-inhibitory effects of A. turcicum Özhatay & Cowley herb extract on human glioblastoma U373 cells, human colorectal carcinoma cell HCT-116, and healthy HUVEC cell lines were determined by the MTT method. After 24 and 48 h of application, logIC50 values in HUVEC, HCT-116, and U373 cells were defined as 3.737, 3.765; 3.513, 3.696, 4.476, and 4.104 μg/mL, respectively. We conducted a cell migration experiment to study the A. turcicum Özhatay & Cowley Extract (ATÖCE) impact on cancer cells’ metastatic behavior. Our findings indicate that ATÖCE has an inhibitory effect on the migration potential of the cells used in the study. We conducted experiments using DPPH, ABTS, CUPRAC, and total phenolic content to assess the antioxidant properties of ATÖCE. The findings from the antioxidant activity experiments revealed an activity level of 0.20 ± 0.046 at IC50. Additionally, the total phenolic content was measured to be 0.26 ± 0.044 mg GAE/g. Copyright © 2024 İPEK, Baran, Cebe, Ahmadian, Eftekhari and Baran.
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    Biosynthesis of silver nanoparticles from Arum dioscoridis plant leaf aqueous extract: anticancer and antimicrobial properties
    (Gültekin ÖZDEMİR, 2023) İpek, Polat; Baran, Mehmet Fırat; Yıldız, Reşit; Hatipoğlu, Abdulkerim
    This study was carried out to synthesize silver nanoparticles (AgNPs) from Arum dioscoridis (AD) leaf extract and to investigate the cytotoxic and antipathogenic effects of them. The plant material had a reducing and stabilizing effect on the synthesized nanomaterial. During the plant-mediated synthesis of nanomaterials, no substances that would cause environmental pollution were used. For the structural characterization of AD-AgNPs, Ultraviolet-visible (UV-vis) Spectroscopy, Field Emission Scanning Electron Microscopy (FE-SEM), Electron Dispersive X-ray (EDX) Spectroscopy, Fourier Transform Infrared (FT-IR) Spectroscopy, Transmission Electron Microscopy (TEM), X-ray Diffractometer (XRD), Atomic Force Microscopy (AFM) and Zetasizer analyses were performed. The produced AgNPs showed maximum surface plasmon resonance at 431.67 nm and had mostly spherical morphology. The zeta potential value of the nanomaterial was -9.76 mV and the average powder crystal size was 31.48 nm. The minimum inhibitory concentration (MIC) values (mg/L) of AD-AgNPs on Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans were 0.25, 2.00, 0.125, 4.00, and 1.00, respectively. After 24 and 48 hours of application by MTT [3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromid] assay, the half-maximal inhibitory concentrations (IC50: μg/mL) of AD-AgNPs on human colon adenocarcinoma cell (CACO-2), human breast cancer cell (MCF-7), glioblastoma multiforme cell (T98-G), and healthy human umbilical vein endothelial cell (HUVEC) lines were determined as 2.977, 2.801, 5.694, 4.392; 2.115, 2.300, 2.612, 4.091, respectively.
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    A case of traumatic myiasisina domestic rabbit ( oryctolagus cuniculus ) caused By lucilia sericata
    (2012) İpek, Duygu Neval Sayın; İpek, Polat
    Lucilia sericata fakültatif travmatik myiasis etkenlerinden biri olup, önemli paraziter enfestasyonlara yol açarak hem insan sağlığı hem de hayvancılık ekonomisine büyük zararlar vermektedir. Dicle Üniversitesi Veteriner Fakültesi kliniklerine sol karpal ekleminde yara şikayeti ile 3 aylık dişi bir tavşan getirilmiştir. Yara muayenesinde yaranın larvalarla enfeste olduğu belirlenmiştir. Yaradan toplanan larvaların mikroskobik incelemesi sonucunda bu larvaların 3. dönem L. sericata larvaları olduğu saplanmıştır.
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    Cytotoxic potential of selenium nanoparticles (SeNPs) derived from leaf extract of Mentha longifolia L.
    (2024) İpek, Polat; Baran, Ayşe; Hatipoğlu, Abdulkerim; Baran, Mehmet Fırat
    The search for alternative methods in cancer treatments has been going on for many years. In the current study conducted for this purpose, selenium nanoparticles (ML-SeNPs) were produced from the aqueous leaf extract of Mentha longifolia L. easily and inexpensively, without harming the environment. The anticancer potential of ML-SeNPs on glioblastoma cell (U373), osteosarcoma cell (U2OS), and healthy retinal pigment epithelial cell (RPE-1) lines was determined by MTT (3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromid) test. For the test, ML-SeNPs were applied at 100, 300, and 600 µg/mL levels and interaction was provided for 24 and 48 hours. The survival rates (%) in RPE-1, U373, and U2OS cell lines in the 24-hour application were 107.49-98.89, 97.66-86.82, and 87.81-83.37, respectively. The viability rates (%) of the cells in 48 hours of application were 72.27-87.39, 68.17-73.48, and 81.00-84.67, respectively. In general, it was discovered that the cytotoxic effect of ML-SeNPs on RPE-1, U373, and U2OS cell lines was greater at low doses and increased over time. In-vivo studies that support the antiproliferative action of ML-SeNPs may boost the prospect of using them as therapeutic agents in potential cancer treatment procedures in the following years.
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    Cytotoxic potential of selenium nanoparticles (SeNPs) derived from leaf extract of Mentha longifolia L.
    (Gültekin ÖZDEMİR, 2024) İpek, Polat; Baran, Ayşe; Hatipoğlu, Abdülkerim; Baran, Mehmet Fırat
    The search for alternative methods in cancer treatments has been going on for many years. In the current study conducted for this purpose, selenium nanoparticles (ML-SeNPs) were produced from the aqueous leaf extract of Mentha longifolia L. easily and inexpensively, without harming the environment. The anticancer potential of ML-SeNPs on glioblastoma cell (U373), osteosarcoma cell (U2OS), and healthy retinal pigment epithelial cell (RPE-1) lines was determined by MTT (3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolium bromid) test. For the test, ML-SeNPs were applied at 100, 300, and 600 µg/mL levels and interaction was provided for 24 and 48 hours. The survival rates (%) in RPE-1, U373, and U2OS cell lines in the 24-hour application were 107.49-98.89, 97.66-86.82, and 87.81-83.37, respectively. The viability rates (%) of the cells in 48 hours of application were 72.27-87.39, 68.17-73.48, and 81.00-84.67, respectively. In general, it was discovered that the cytotoxic effect of ML-SeNPs on RPE-1, U373, and U2OS cell lines was greater at low doses and increased over time. In-vivo studies that support the antiproliferative action of ML-SeNPs may boost the prospect of using them as therapeutic agents in potential cancer treatment procedures in the following years.
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    Deciphering the effect of Potentilla fulgens root extract against healthy HUVEC cell line and cancer cell lines (A549 and SKOV-3)
    (Elsevier B.V., 2024) İpek, Polat; Baran, Ayşe; Baran, Mehmet Fırat; Eftekhari, Aziz; Khusro, Ameer; Ommati, Mohammad Mehdi; Aliyev, Elvin; Khalilov, Rovshan
    Background: Potentilla fulgens, a highly valued indigenous medicinal herb grown in high altitudes of the Himalayan region with anticancer, hypoglycaemic, antibacterial, anti-inflammatory, and antiulcerogenic properties, are used in traditional systems of medicine. This study was aimed to investigate the effect of P. fulgens root extract, as one of the natural alternatives to chemotherapeutic drugs used in cancer treatment, on proliferation, apoptosis, and autophagy of human non-small cell lung cancer cell line (A549), human ovarian cancer cell line (SKOV-3), and healthy human umbilical vein endothelial cell line (HUVEC). Methods: Anti-proliferative effect was assessed by MTT assay. The expression of autophagy and apoptosis-related proteins was evaluated by western blotting. Total oxidant status (TOS) and total antioxidant capacity (TAC) test were determined using standard kit methods. Results: Our results showed that the extract inhibited proliferation of HUVEC, A549, and SKOV-3 cells in a dose-dependent manner. MTT assay analysis revealed that the extract significantly (P<0.05) induced mortality in HUVEC, A549, and SKOV-3 cells. Western blot results revealed increased expression of NF-κB after the extract treatment but led to the down-regulation in Beclin-1, Bax, extracellular-signal-related kinase 1 and 2, Sequestosome-1, and cleaved Casp-3 levels. Treatment groups showed an increase in TOS and TAC values in A549 and SKOV-3 cell lines, while HUVEC cell line showed an increase in TAC and a decrease in TOS values, compared to the control group. Conclusions: Our findings indicated that P. fulgens root extract inhibited the proliferation of healthy cells and cancer cells through cell cycle arrest, representing its limited application as therapeutic agent in cancer treatment.
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    Determination of chemical components of the endemic species Allium turcicum L. plant extract by LC-MS/MS and evaluation of medicinal potentials
    (Elsevier Ltd, 2024) İpek, Polat; Atalar, Mehmet Nuri; Baran, Ayşe; Baran, Mehmet Fırat; Ommati, Mohammad Mehdi; Karadağ, Musa; Zor, Murat
    The Allium turcicum L. (Zuzubak) plant as a cultivated vegetable have various health benefits and consumed as a food. Due to the shortcoming evidence in literature and the importance of this plant in folk medicine, in the present study, for the first time, we evaluated the bioactive profile of components (using LC-MS/MS), cytotoxicity, anticancer, antioxidant, and antibacterial prospectives of Zuzubak methanol extract. Reported results show that the extract is rich in bioactive compounds and has anticancer activity with breast cancer cells (MCF-7), human prostate cancer cells (DU-145), and Human osteosarcoma cancer Cell lines of (IC50) in dose dependent manner in the concentration range of 31.25 ?g/mL and 2000 ?g/mL for 24 and 48 h. Western blotting results determined that the extract significantly suppressed the growth of U2OS, MCF-7, and DU-145 cancer cells by down expression of Ang-1 (angiogenic protein) and Beclin-1 (autophagy protein) and overexpression of Bax (a proapoptotic protein). The oxidative stress indices showed a reduction in RPE-1 and MCF-7 cells and an upsurge in U2OS and DU-145 cells. Additionally, the antimicrobial assay showed suppression of the growth of various pathogenic microorganisms in 4.00–8.00 ?g/concentrations of Zuzubak extract using the microdilution method. The phytochemicals identified showed promising anticancer, antioxidant effects, and antimicrobial properties, representing a valuable herbal source for drug development studies. © 2024 The Authors
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    Diyabet oluşturulan sıçanlarda potentilla fulgens’in etkilerinin diğer antidiyabetiklerle karşılaştırılması
    (2015) İpek, Polat
    Diyabet Oluşturulan Sıçanlarda Potentilla fulgens’in Etkilerinin Diğer Antidiyabetiklerle Karşılaştırılması Diabetes Mellitus (DM) insülin sekresyonu ve/veya insülin etkisinin mutlak veya göreceli eksikliği sonucu karbonhidrat, lipit ve protein metabolizmasında bozukluklara yol açan kronik hiperglisemi ile karekterize etiyolojisinde birden fazla faktörün rol oynadığı metabolik bir hastalıktır. Kontrolsüz diyabetten kaynaklanan hiperglisemi; akut komplikasyonlar ile ölüme yol açabildiği gibi, uzun süren metabolik düzensizlikler nedeniyle, çeşitli organların çalışmasında yetersizlik ve işlevsizlik şeklinde kronik komplikasyonlara da neden olabilmektedir. Bu hastalık; tüm yaş gruplarında görülür. Ülkemizde de halen beş milyonun üzerinde vatandaşımızı etkilediği düşünülmektedir. Çeşitli komplikasyonlarla seyrettiği, iyi tedavi edilmediğinde kalp, damar, göz, böbrek ve sinir dokusu başta olmak üzere hemen tüm yaşamsal organlarda kalıcı bozukluklara neden olabildiği ve yaşam kalitesini önemli ölçüde azalttığı için, tedavi maliyetinin çok yüksek olduğu bulaşıcı olmayan bir sağlık sorunudur. Bütün bu özellikleri nedeniyle DM, dünyadaki hemen hemen tüm ülkelerin sağlık politikalarında özel bir önem taşımaktadır. Diyabete bağlı komplikasyonları önlemek veya azaltmak için çeşitli ilaçlar kullanılmaktadır. Bunlar arasında en çok kan glikoz ve lipit düzeyini regüle eden ilaçlar ve renin anjiotensin anatagonistleri yer almaktadır. Tüm bu farmakolojik tedavi yaklaşımlarının etkileri sınırlıdır ve yüksek dozlarda hipoglisemi, karaciğer toksisitesi, laktik asidozis ve diyare oluşturmakta ve uzun süreli kullanılmasına bağlı olarak etkileri zayıflamaktadır. Potentilla fulgens; beşparmakotu olarak adlandırılan yerel, yenilebilir, kısa, ince bitkidir. Hindistan'da halk arasında peptik ülser, ağız ülseri, diyare, diyabet ve kanser gibi çeşitli hastalıklara karşı kökleri çiğnenerek kullanılmaktadır. Bu bitkinin hipoglisemik etkileriyle ilgili literatürde birkaç çalışma yapılmış olduğu görülmektedir. Bu durum Potentilla fulgens’in hypoglisemik etkinliğinin araştırılması ve diğer antidiyabetiklerle karşılaştırılmasının yararlı olacağı düşüncesini akla getirmiştir. Bu amaçla, toplamda 49 adet erişkin sprague dawley sıçan her bir grupta 7 adet olacak şekilde 7 gruba ayrıldı; 1.Kontrol, 2. Diyabetik 3. Gliklazid, 4.Metformin, 5. Potentilla fulgens 450 oral, 6. Potentilla fulgens 900 oral, 7. Potentilla fulgens 450 i.p. Kontrol dışındaki gruplara diyabet oluşturmak için streptozotosin 55mg/kg tek doz intraperitoneal (i.p.) olarak uygulandı. Deney gruplarına metformin (500mg/kg), gliklazid (5mg/kg), Potentilla fulgens 450 ve 900 mg/kg orogastrik olarak, 7.gruba ise Potentilla fulgens 450 mg/kg i.p. olarak uygulanırken kontrol ve diyabetik kontrol gruplarına aynı yoldan plasebo verildi. Deney periyodu sonunda alınan kan örneklerinde kan şekeri ve karaciğer örneklerinde; Fruktoz 1,6 di fosfataz, glikoz 6 fosfat, glikoz 6 fosfat dehidrogenaz, hekzokinaz ve piruvat kinaz enzim aktivitileri elisa okuyucu ile ölçüldü. Potentilla fulgens i.p. ve 900 mg/kg oral tedavi uygulamamız diyabetik sıçanların kan şekeri düzeyleri ve karaciğer enzimleri üzerine olumlu etkiler gösterirken (p<0.05) 450 mg/kg oral tedavi dozumuz önemli olumlu etkiler göstermemiştir (p>0.05). Kan şekeri ve karaciğer enzimlerine etkileri yönünden karşılaştırıldığında gliklazid metformine göre daha etkili bulunmuştur (p<0.05). Anahtar Kelimeler: Potentilla fulgens, streptozotosin, sıçan, diyabet, metformin, gliklazid
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    Diyarbakır ilinde mezbahada kesilen keçilerde keçi nokrasının prevalansı
    (Dicle Üniversitesi Veteriner Fakültesi, 2017) İpek, Duygu Neval Sayın; Ünver, Özkan; İpek, Polat
    Bu çalışma Kasım 2011-Mart 2012 tarihleri arasında Diyarbakır ilinde keçi nokrasının görülme oranı hakkında bilgi edinmek için mezbahada kesilen 225 keçi üzerinde yürütüldü. Kesilen keçiler nokra yönünden muayene edilerek enfeste olduğu tespit edilen hayvanlardan larvalar toplanarak her hayvanın derisindeki delik sayıları protokollere yazıldı. Toplanan larvalar laboratuvarda incelenerek literatürler ışığında bütün larvaların Przhevalskiana silenus oldukları belirlendi. Muayene edilen keçilerde keçi nokrasının görülme oranı %5.3 (12/225) olarak belirlenirken derilerde larvaların neden olduğu delik sayısının 1-7 arasında olduğu tespit edildi. Erkeklerdeki enfestasyon oranı %4.7 (9/189) iken dişilerde bu oran %8.3 (3/36) olarak görüldü (P>0,05). Bu çalışmanın sonuçlarına dayanarak, keçilerde P. silenus’un neden olduğu nokra ile ilgili daha detaylı çalışmaların yapılması ve koruma-kontrol programlarının geliştirilmesinin yararlı olabileceği kanısına varıldı.
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    Green synthesis and characterization of selenium nanoparticles (Se NPs) from the skin (testa) of Pistacia vera L. (Siirt pistachio) and investigation of antimicrobial and anticancer potentials
    (Springer Science and Business Media, 2023) Baran, Mehmet Fırat; Keskin, Cumali; Baran, Ayşe; Kurt, Kadri; İpek, Polat; Eftekhari, Aziz; Khalilov, Rovshan; Fridunbayov, Ismayil; Cho, William C.
    Metallic nanoparticles created by ecologically friendly synthesis processes are becoming increasingly useful in a variety of applications. Because of their strong bioactive component qualities, biocompatible architectures, high stability, and low toxicity, green-produced selenium nanoparticles are particularly signifcant materials for various medicinal applications. Plants include a wealth of essential phytochemicals with therapeutic and medical capabilities. Pistachio vera L. (Siirt pistachio) is a seasonal fruit that is frequently consumed for its nutritional worth and health advantages. However, the outer colored skin of the P. vera (Siirt pistachio) fruit, which is not consumed, contains many biologically active compounds. In this study, plant-mediated synthesis of selenium nanoparticles (SeNPs) was successfully accomplished after adding the sodium selenite solution to the aqueous extract of P. vera colored skin waste. The synthesized Se NPs were characterized with UV–Vis, transmission electron microscopy (TEM), dynamic light scattering (DLS), zeta distribution, atomic force microscope (AFM), Fourier transform infrared spectrum (FTIR), thermogravimetric (TGA) and diferential thermal analysis, X-ray difraction (XRD), and energy-dispersive X-ray spectrum (EDX). Biogenic Pistacia vera (PV) PV-SeNPs were also tested for their ability to suppress the development of various pathogenic bacteria and cancerous cell lines. The UV-visible (UV–Vis) spectra revealed an absorption peak at 325 nm, which refected the surface plasmon band. The signifcant selenium signal on the EDX spectrum at 1.5 keV confrmed the creation of Se NPs. The presence of several peaks on the FTIR spectrum of the aqueous extract of Pv and the nanoparticles indicated the presence of some important functional groups such as amines, carbonyl compounds, and phenols, which are important in facilitating the process of capping and bioreduction, as well as conferring stability to nanoparticles. The TEM microphotographs revealed that the nanoparticles were highly distributed, had a spherical morphological form, and were monodisperse below 10 nm. Biogenic Pv-SeNPs exhibited similar antimicrobial activity as standard antibiotics. However, it was determined that the cytotoxic activity of Se-NPs against cancer cell lines was quite high depending on the dose and time. As a result, the Pv-SeNPs are likely to be extremely benefcial in the pharmaceutical and cosmetic sectors, as well as in the food and cosmetic industries, in producing antimicrobial and/or anticancer medications.
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    Green synthesis and evaluation of antipathogenic, antioxidant, and anticholinesterase activities of gold nanoparticles (Au NPs) from Allium cepa L. peel aqueous extract
    (Springer Science and Business Media, 2023) İpek, Polat; Baran, Mehmet Fırat; Baran, Ayşe; Hatipoğlu, Abdulkerim; Keskin, Cumali; Yıldıztekin, Mahmut
    Gold nanoparticles (Au NPs) have potentially therapeutic properties as they are synthesized via biomolecules as reducing and stabilizing agent(s). The aim of this study is to develop an easy and eco-friendly method for the synthesis of Au NPs using extracts from the Allium cepa (AC) red peel (skin) extract and investigate the antibacterial and antioxidant activity and also inhibitory effects on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. UV–Vis peak at ~ 564 nm confirmed the Au NPs absorbance. TEM images revealed the formation of Au NPs with mostly spherical shapes and sizes between 6.08 and 54.20 nm. FTIR analysis confirmed the important biological compounds responsible for the reduction of gold. The strong absorption property of Au NPs was studied by EDX. The produced Au NPs demonstrated significant antibacterial and antifungal activity against the bacterial and fungal strains tested, as well as efficient inhibitory activity against both AChE and BChE enzymes. The highest antimicrobial activities were found against Staphylococcus aureus (0.06° mg/ml) and Candida albicans (0.06° mg/ml). The antioxidant test findings revealed that AC-Au NPs had lesser activity when compared to normal antioxidants. The Au NPs showed excellent inhibitory efficacy against AChE and BChE. The proposed green technique could encourage the innocuous generation of Au NPs, implying therapeutic possibilities.
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    Green synthesis of silver nanoparticles derived from papaver rhoeas L. Leaf Extract: Cytotoxic and antimicrobial properties
    (Multidisciplinary Digital Publishing Institute (MDPI), 2023) İpek, Polat; Yıldız, Reşit; Baran, Mehmet Fırat; Hatipoğlu, Abdulkerim; Baran, Ayşe; Sufianov, Albert; Beylerli, Ozal
    In the last few decades, the search for metal nanoparticles as an alternative to cancer treatments and antibiotics has increased. In this article, the spectroscopic (ultraviolet–visible (UV-vis), electron-dispersing X-ray (EDX), and Fourier transform infrared (FT-IR)), microscopic (field emission scanning electron microscope (FE-SEM), transmission electron microscope (TEM), and atomic force microscope (AFM)), structural (X-ray diffractometer (XRD) and zetasizer), and analytic (thermogravimetric/differential thermal analyzer (TGA-DTA)) characterization of the silver nanoparticles (AgNPs) produced from Papaver rhoeas (PR) L. leaf extract are presented. PR-AgNPs are generally spherical and have a maximum surface plasmon resonance of 464.03 nm. The dimensions of the manufactured nanomaterial are in the range of 1.47–7.31 nm. PR-AgNPs have high thermal stability and a zeta potential of −36.1 mV. The minimum inhibitory concentration (MIC) values (mg L−1) of PR-AgNPs on Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans are 1.50, 0.75, 3.00, 6.00, and 0.37, respectively. In the study, the cytotoxic and proliferative effects of PR-AgNPs using the MTT (3-(4,5-dimethylthiazol-2-yl)-diphenyltetrazolium bromide) method on various cancer cell lines (CACO-2 (human colon adenocarcinoma cell), MCF-7 (human breast cancer cell), T98-G (glioblastoma multiforme cell), and healthy HUVEC (human umbilical vein endothelial cell)) cell lines are presented. After 24 and 48 h of the application, the half-maximum inhibitory concentration (IC50) values (μg mL−1) of PR-AgNPs on HUVEC, CACO-2, MCF-7, and T98-G lines are 2.365 and 2.380; 2.526 and 2.521; 3.274 and 3.318; 3.472 and 3.526, respectively. Comprehensive in vivo research of PR-AgNPs is proposed to reveal their potential for usage in sectors such as nanomedicine and nanochemistry.
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    Green-synthesized nanoparticles for biomedical sensor technology
    (Elsevier, 2024) Baran, Ayse; Baran, Mehmet Fırat; İpek, Polat; Eftekhari, Aziz; Keskin, Cumali; Atalar, Mehmet Nuri; Alma, Mehmet Hakkı
    Sensor technology is an integral part of the many cost-effective and efficient factors possible in modern medical devices. Biosensors have good potential as they are easy, scalable, and effective in manufacturing processes. Nanotechnology has become one of the promising technologies applied in all fields of science. Biotechnologically produced metallic nanoparticles (NP) attract attention in scientific applications and technology platforms due to their extensive applications in biomedical and physiochemical fields. In recent years, the side effects caused by the use of synthetic drugs and the medical and economic problems caused by them have made the use of plants popular again. Ecofriendly, nontoxic metal-based NPs (such as gold, silver, palladium, manganese, and zinc) smaller than 100nm in size can be synthesized with extracts obtained from plants by different methods. As the sizes and shapes of NPs change, the physical, chemical, bioactive, optical, electrical, catalytic, and toxicity properties of the particles also change. Biological synthesis, also known as green synthesis, is a practical method to obtain NPs easily and ecologically without the need for high pressure, high-temperature values, and toxic chemicals. Green synthesis of NPs is carried out using different biomaterials such as bacteria, fungi, yeast, viruses, microalgae, and plant biomass/essence. Plant-mediated biosynthesis of metallic NPs occurs through biomolecules containing organic functional groups in the plant. Nanobiosensors, analytical devices combining a biologically sensitive element with a nanostructured transducer, are widely used for the molecular detection of biomarkers associated with the diagnosis of diseases and the detection of infectious organisms. Nanobiosensors show certain advantages over laboratory and many field methods due to their inherent specificity, simplicity, and rapid response. In this study, advancements in the development of nanobiosensors are illuminated. Considering all these aspects, it can be said that nanobiosensors enable diagnostic tools with increased sensitivity, specificity, and reliability for medical applications. © 2025 Elsevier Inc. All rights reserved.
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    Investigation of possible antidiabetic effects of potentilla fulgens in diabetic rats and comparison with other antidiabetics
    (Haydarpaşa Numune Eğitim ve Araştırma Hastanesi, 2019) İpek, Polat; Taşdemir, Ezel; Koçyiğit, Yüksel
    Introduction: The current antidiabetic drugs have adverse side effects and the effects may decrease overtime depending oncontinuous use. In recent years, phytochemicals of natural plant origin, which show antidiabetic properties and have fewerside effects, have been the subject of research. However, studies and clinical studies on the antidiabetic and hypoglycemiceffects of Potentilla fulgens are still not enough. We aimed to examine the antidiabetic effects of P. fulgens in diabetic ratsand to contribute to new treatment approaches by comparing them with other antidiabetics.Methods: In this study, rats were divided into seven groups, one control and six diabetic groups as seven rats in each group.The rats in which diabetes was induced by streptozotocin were sacrificed after treatment with two different doses of intraperitonealand intragastric P. fulgens and standard antidiabetic drugs, metformin and gliclazide for 3 weeks.Results: Intraperitoneal administration of P. fulgens significantly improved the activity of liver enzymes related to fastingblood glucose levels and carbohydrate metabolism. Intragastric 450 mg/kg/day P. fulgens did not show adequate antidiabeticeffects. However, P. fulgens administered twice the usual dose (900 mg/kg/day) caused significant antidiabetic effects.Compared with metformin and gliclazide, it was found that P. fulgens had similar effects at high doses.Discussion and Conclusion: According to our findings, P. fulgens improves the activity of liver enzymes related to bloodglucose and glucose metabolism in diabetic rats and has significant antidiabetic effects.
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    Oestrus ovis larvaları ile enfekte ivesi koyunlarda lipit peroksidasyonun, toplam antioksidan kapasitesinin ve bazı hematolojik parametrelerin değerlendirilmesi
    (Dicle Üniversitesi Veteriner Fakültesi, 2023) İpek, Duygu Neval Sayın; İpek, Polat; Şimşek, Aynur; Koçhan, Akın; Katanalp, Ömer Faruk
    Oestrosis, koyun ve keçilerin burun boşluğu ve frontal sinuslarına yerleşip gelişen Oestrus ovis sineğinin birinci, ikinci ve üçüncü dönem larvalarının neden olduğu bir nazofarengeal miyazdır. Bu çalışmanın amacı Oestrus ovis larvaları ile doğal enfeste ivesi koyunlarda lipid peroksidasyon, total antioksidan kapasite ve bazı hematolojik parametreler üzerine olan etkisini araştırmaktır. Kırk ivesi koyun üzerinde yürütülen çalışmada Oestrus ovis enfestasyonlarının moleküler tanısı için koyunların her iki burun deliğinden steril pamuklu swaplar ile mukus numuneleri alındı. Lipid perokidasyonu ve total antioksidan kapasite seviyeleri ile total lökosit sayısı, eritrosit sayısı, hematokrit değer ve hemoglobin konsantrasyonunun belirlenmesi için koyunların vena jugularislerinden kan örnekleri alındı. Kırk swap örneğinden 35’inin semi-nested PCR yöntemi kullanılarak enfestasyon yönünden pozitif olduğu tespit edildi. Enfeste olduğu tespit edilen hayvanlar burun akıntısının şiddetine göre iki gruba ayrıldı (grup 1: hafif burun akıntısı olanlar; grup 2: şiddetli burun akıntısı olanlar). Grup 1 ve 2’de serum lipid peroksidasyonu artışının kontrol grubuna göre istatiksel olarak önemli olduğu belirlendi (p˂0.05). Grup 1 ve grup 2 kontrol grubu ile kıyaslandığında total antioksidan kapasitede azalma görülmesine rağmen, kontrol grubu ile aralarındaki farkın istatiksel olarak önemli olmadığı belirlendi (p>0.05). Hematolojik parametrelerde gruplar arasında önemli farklılık bulunmadı (p>0.05). Sonuç olarak, koyunlarda Oestrus ovis larvalarının neden olduğu doku hasarı, lipid peroksidasyonun artışına neden olabileceği kanısına varıldı