Effects of Potentilla Fulgens as a Prophylactic Agent in Testicular Ischemia-Reperfusion Injury

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dc.contributor.authorKarabulut, Ozlen
dc.contributor.authorKalkanli, Sevgi Tas
dc.contributor.authorDeveci, Engin
dc.contributor.authorIsen, Kenan
dc.contributor.authorUysal, Ersin
dc.contributor.authorSoker, Ipek Sevda
dc.date.accessioned2024-04-24T17:47:29Z
dc.date.available2024-04-24T17:47:29Z
dc.date.issued2016
dc.departmentDicle Üniversitesien_US
dc.description.abstractOBJECTIVE: To study the protective effects of Potentilla fulgens extract on damage of testicular tissue created by ischemia and reperfusion treatment via histopathological, immunohistochemical, and TUNEL experiments. STUDY DESIGN: In this study 24 Sprague-Dawley male rats were divided into 4 subgroups: control group, torsion group, torsion-detorsion group, and Potentilla fulgens +torsion-detorsion group. The extract of Potentilla fulgens was intraperitoneally injected into the rats, 400 mg/kg for 5 days. The right testicle was exposed to torsion and detorsion for 2 hours with a 720 turn. The right testicle was dissected from the scrotum after the rats were anesthetized with ketamine hydroxide. Following fixation of 10% formaldehyde solution, routine tissue processing protocol was applied. Immunohistochemically slides were stained with TNF-alpha antibody. Apoptotic changes were examined using the TUNEL protocol. RESULTS: In this study seminiferous tubule diameter and depth of spermatic cells were compared among the experimental and control groups. Histopathologic examination shows that degeneration and disorganization of the spermatic cells and changes in tubule diameter were found to be statistically significant. In addition, histopathological examination of the apoptotic indices was scored using the TUNEL method. Immunohistochemically, TNF-a expression was positively observed in the ischemia and the ischemia-reperfusion groups. CONCLUSION: As a result of torsion and detorsion exposure, which led to damage in spermatic cells of seminiferous tubules, Potentilla fulgens was shown to decrease apoptotic development and was thought to be the cause of activation in spermatic cells.en_US
dc.identifier.endpage312en_US
dc.identifier.issn0884-6812
dc.identifier.issue5en_US
dc.identifier.startpage306en_US
dc.identifier.urihttps://hdl.handle.net/11468/22542
dc.identifier.volume38en_US
dc.identifier.wosWOS:000387093200009
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.language.isoenen_US
dc.publisherSci Printers & Publ Incen_US
dc.relation.ispartofAnalytical and Quantitative Cytopathology and Histopathology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCinquefoilen_US
dc.subjectIschemia-Reperfusion Injuryen_US
dc.subjectPotentillaen_US
dc.subjectPotentilla Fulgensen_US
dc.subjectRatsen_US
dc.subjectReperfusion Damageen_US
dc.subjectReperfusion Injuryen_US
dc.subjectRosaceaeen_US
dc.subjectSilverweeden_US
dc.subjectSpermatic Cord Torsionen_US
dc.subjectTesticular Torsionen_US
dc.subjectTestisen_US
dc.titleEffects of Potentilla Fulgens as a Prophylactic Agent in Testicular Ischemia-Reperfusion Injuryen_US
dc.titleEffects of Potentilla Fulgens as a Prophylactic Agent in Testicular Ischemia-Reperfusion Injury
dc.typeArticleen_US

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