The Importance of Indirect Fluoresan Antibody Test in the Diagnosis of Cystic Echinococcosis

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dc.contributor.authorAkpolat, Nezahat
dc.contributor.authorGedik, Ercan
dc.date.accessioned2024-04-24T17:40:44Z
dc.date.available2024-04-24T17:40:44Z
dc.date.issued2009
dc.departmentDicle Üniversitesien_US
dc.description.abstractObjective: Cystic echinococcosis (CE) is a widespread human infection caused by the larval stage of the taeniid tapeworm Echinococcus granulosus. The disease is well known in sheep-herding areas of Asia, Africa and South Central America. This parasitic infection is relatively frequent around the Mediterranean Sea. The clinical features are non-specific. Many serologic techniques can be used to detect or quantify humoral immunity. Their sensitivity and specificity, depending on the quality of antigens, are well known. The aim of this study was to evaluate the utility of indirect immunofluorescent-antibody assay in the diagnosis of CE. Material and Methods: This study was conducted in the Microbiology Department of the Dicle University Medical Faculty and Genaral Surgical Unit of the same hospital between May 2006 and June 2008. In this study, we evaluated the serum samples of 54 non-GE patients and 77 serum samples of patients with a clinical and radiological diagnosis of CE and who were operated later. The specific IgG antibody was investigated in 131 serum samples by indirect immunofluorescent-antibody assay. The study was performed by Anti-Echinococcus granulosus IIFT commercial kit using the frozen sections of Echinoccocus larvae. Results: E. granulosus IgG antibodies were investigated in 131 serum samples. Of the samples, 81 (61.83%) were seropositive and 50 (38.16%) were seronegative. Among 81 serum samples which were serologically positive, 70 (86.41%) were from patients with a radiological and clinical diagnosis of CE and 11 (13.58%) were from patients without a previous diagnosis. Seven seronegative samples were from CE patients. The mean age of the patients was 35.78 (8-83) and of the 77 CE patients, 55 (71.42%) were female, 22 (28.57%) were male; the infection was localized in the liver in 93.5% of the cases. The sensitivity and the specificity of the IFA were 91.6% and 83.0% respectively. The positive and negative predictive values of the test were 87% and 88.5%, respectively. Conclusion: Indirect immunofluorescent-antibody assay is a sensitive and specific test, especially in liver-localized CE. The assay is easy to run and provides an advantage of rapid diagnosis. The test may be used confidently along with radiological diagnostic methods.en_US
dc.identifier.endpage1597en_US
dc.identifier.issn1300-0292
dc.identifier.issn2146-9040
dc.identifier.issue6en_US
dc.identifier.startpage1594en_US
dc.identifier.urihttps://hdl.handle.net/11468/21929
dc.identifier.volume29en_US
dc.identifier.wosWOS:000273915500034
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.language.isotren_US
dc.publisherOrtadogu Ad Pres & Publ Coen_US
dc.relation.ispartofTurkiye Klinikleri Tip Bilimleri Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEchinococcus Granulosusen_US
dc.subjectFluorescent Antibody Technique, Indirecten_US
dc.subjectEchinococcosisen_US
dc.titleThe Importance of Indirect Fluoresan Antibody Test in the Diagnosis of Cystic Echinococcosisen_US
dc.titleThe Importance of Indirect Fluoresan Antibody Test in the Diagnosis of Cystic Echinococcosis
dc.typeArticleen_US

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