Evaluation of the Ehrlich-Ziehi-Neelsen (EZN) and Amplified Mycobacterium tuberculosis Direct Test according to the BACTEC method in respiratory and nonrespiratory samples

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dc.contributor.authorOzekinci, Tuncer
dc.contributor.authorMese, Sevinn
dc.contributor.authorAtmaca, Selahattin
dc.contributor.authorAkpolat, Nezahat
dc.contributor.authorGuel, Kadri
dc.date.accessioned2024-04-24T17:44:30Z
dc.date.available2024-04-24T17:44:30Z
dc.date.issued2007
dc.departmentDicle Üniversitesien_US
dc.description.abstractAim: Tuberculosis remains a significant and threatening disease, particularly in developing countries. Mycobacterium tuberculosis should be detected and identified as soon as possible to ensure the prevention of the spread of the disease. For this purpose, use of fast and reliable laboratory diagnostic methods with high sensitivity and specificity was initiated in recent years. Materials and Methods: In this study, 107 respiratory and 198 norrespiratory (305 in total) samples submitted to Dicle University Faculty of Medicine Clinical Microbiology Laboratory were examined using the Ehrlich-Ziehl-Neelsen (EZN), BACTEC 460 TB (Becton and Dickinson Diagnostic Instrument System, Towson, MID), and MTD (Amplified Mycobacterium tuberculosis Direct Test, Gen-Probe, USA) methods. Results: In respiratory samples, sensitivity of EZN was found as 83.33%, specificity as 95.04%, positive predictive value as 50%, and negative predictive value as 98.96%, whereas in nonrespiratory samples these values were 18.18%, 98.39%, 40%, and 95.37%, respectively. In respiratory samples, sensitivity of MTD was found as 83.33%, specificity as 94.05%, positive predictive value as 45.45%, and negative predictive value as 98.95%, whereas in nonrespiratory samples these values were 54.54%, 88.23%, 21.42%, and 97.05%, respectively. Conclusions: In view of the above, the pre-diagnostic EZN test and the MTD method based on nucleic acid amplification should be applied together with the BACTEC 460 system, which is considered as a gold standard, and the evaluation should be made accordingly. Furthermore, MTD should not be used as a screening test due to its high cost, and should rather be preferred in smear-positive samples.en_US
dc.identifier.endpage149en_US
dc.identifier.issn1300-0144
dc.identifier.issn1303-6165
dc.identifier.issue3en_US
dc.identifier.startpage145en_US
dc.identifier.urihttps://hdl.handle.net/11468/22113
dc.identifier.volume37en_US
dc.identifier.wosWOS:000254615700006
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Medical Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectTuberculosisen_US
dc.subjectAmplified Mycobacterium Tuberculosis Direct Testen_US
dc.subjectBactecen_US
dc.subjectEhrlich-Ziehi-Neelsenen_US
dc.titleEvaluation of the Ehrlich-Ziehi-Neelsen (EZN) and Amplified Mycobacterium tuberculosis Direct Test according to the BACTEC method in respiratory and nonrespiratory samplesen_US
dc.titleEvaluation of the Ehrlich-Ziehi-Neelsen (EZN) and Amplified Mycobacterium tuberculosis Direct Test according to the BACTEC method in respiratory and nonrespiratory samples
dc.typeArticleen_US

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