Değişik yaş gruplarında epstein-barr virüs antikorlarının ıfa yöntemiyle araştırılması
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Dosyalar
Tarih
2007
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Dicle Üniversitesi, Tıp Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
EBV, çocukluk yaslarında sık görülen ve genellikle asemptomatik infeksiyon yaparak vücuttalatent kalan herpesvirüs ailesinin bir üyesidir. Virus potansiyel onkojenik özellikleri ve özellikleimmünyetmezlikli hastalarda agır klinik tablolar olusturmasıyla da önemlidir. Literatürlerde virüsünçesitli hastalıkların patogenezinde rol alabilecegine dair yayınlar gittikçe artmaktadır. Arastırmalardaizole toplumlarda bile EBV antikorlarına sıklıkla rastlandıgı bildirilmektedir. EBV infeksiyonlarıfarklı ülkelerde ve farklı sosyoekonomik düzeylerdeki gruplarda farklı yaslarda kazanılmakta ve budurum klinik prezentasyonu etkilemektedir. Bu çalısmada EBV infeksiyon süpheli hasta serumlarındaspesifik antikorların serolojik profillerinin incelenmesi planlandı. Bu amaçla;1. Degisik yaslardaki bireylerde EBV-VCA IgG ve IgM, EA, EBNA antikorları ve VCA-IgGavidite2. Antikor pozitiflik oranlarının cinsiyet ve yasla iliskisi3. nfeksiyon sekline göre saptanmıs farklı EBV serolojik profilleri incelendi.Çalısma iki yıllık periyot içinde Dicle Üniversitesi Tıp Fakültesi Mikrobiyoloji ve KlinikMikrobiyoloji Anabilim Dalı’nda yürütüldü. Hasta serumlarındaki antikorların varlıgı FA yöntemi ilearastırıldı. Çalısmaya 200 kız, 322 erkek olmak üzere, 0-17 yas ve 17 yas üstü toplam 522 birey alındı.522 hastanın 428’i (%81,99) seropozitif, 94’ü (%18,0) seronegatif bulundu. En çokseropozitiflik oranı 6-12 yas (% 40,89) arasındaydı. Özellikle 3-12 yas aralıgındaki çocuklarda %64,48 olarak bulundu. 12 yas grubuna kadar antikor sıklıgının tedricen arttıgı, 13-17 yas aralıgında %7,71’e düstügü ve 17 yas üstü bireylerde seropozitiflik oranının % 25,7’ ye çıktıgı görüldü. Yasa göreantikor sıklıgının degistigi saptandı. Farklı antijen sınıflarına karsı olusan reaksiyonların belirlenmesifarklı EBV serolojik profillerinin ortaya çıkmasına neden olmustur. Hastaların 265’inde (%50,8)geçirilmis infeksiyon, 116’sında (%22,2) primer infeksiyon, 94’ünde (%18) EBV’ ye duyarlılık,47’sinde (%9) reaktivasyon veya kronik infeksiyon bulundu.EBV serolojisinin kullanılması primer, geçirilmis, reaktive ve EBV’ye hassas bireyleriayırmada yardımcı olur. Epidemiyolojik faktörlerin bilinmesiyle potansiyel onkojen virusun önlenmesiyönündeki çalısmalar aydınlatılacaktır.
EBV is a member of the family of the human herpes viruses family usually affectsduring childhood, causing asymptomatic infections and stay latent in human body. The virusis important because of potential oncogenic feature and especially causing severe clinicaloutcomes among immunocompromised patients. Publications announcing the virus as anethologic agent are increasing. Researchs declare that the antibodies of EBV are frequentlyidentified even among isolated populations. EBV infects groups in different countries, atdifferent ages and socioeconomical levels and this condition affects the clinical population. Inthis study, it was planned to examine the specific antibody profiles among the sera of patientswho were clinically suspected as affected by EBV. For this aim;1. EBV-VCA IgG and IgM, EA, EBNA anibodies and VCA-IgG avidity amongindividuals at different ages2. The relation between antibody positivity rates and sex and age3. Different EBV serological profiles investigated according to detected type ofinfection.This study conducted in Microbiology and Clinical Microbiology Department of DicleUniversity during two years period. The existence of antibodies in patients’ sera wereinvestigated by IFA method in the same department of the same faculty. A total number of522 individuals attended to the study and 200 of them were female, 322 of them were male.A total number of 428 (81.99%) of 522 patients were seropositive, 94 (18%) of themwere seronegative. The most frequent rate of seropositivity was among 6-12 (40.89%) ages.The rate was 64.48%, among the children especially between 3 and 12 years of age. Theantibody frequency were growing through 12 years of age, decreased to 7.71%, between 13-17 of ages and among the group of older than 17 years the rate has found as 25.7%. It wasfound that the antibody frequency has changed by age. Defining the reactions against to thedifferent antigene classes has lead to determine the EBV’s serological profiles.The rate of past infection was 50.76% (n=265), the primer infection rate was22.2%(n=116) and the reactivation or chronic infection rate was found as 9%(n=47).Using the EBV serology, may asist to diverse the primary infection, past infection,reactivation and individuals sensitive to EBV. By knowing the epidemiologic factors, thestudies for preventing against the potentially oncocenic virus will become more clear.
EBV is a member of the family of the human herpes viruses family usually affectsduring childhood, causing asymptomatic infections and stay latent in human body. The virusis important because of potential oncogenic feature and especially causing severe clinicaloutcomes among immunocompromised patients. Publications announcing the virus as anethologic agent are increasing. Researchs declare that the antibodies of EBV are frequentlyidentified even among isolated populations. EBV infects groups in different countries, atdifferent ages and socioeconomical levels and this condition affects the clinical population. Inthis study, it was planned to examine the specific antibody profiles among the sera of patientswho were clinically suspected as affected by EBV. For this aim;1. EBV-VCA IgG and IgM, EA, EBNA anibodies and VCA-IgG avidity amongindividuals at different ages2. The relation between antibody positivity rates and sex and age3. Different EBV serological profiles investigated according to detected type ofinfection.This study conducted in Microbiology and Clinical Microbiology Department of DicleUniversity during two years period. The existence of antibodies in patients’ sera wereinvestigated by IFA method in the same department of the same faculty. A total number of522 individuals attended to the study and 200 of them were female, 322 of them were male.A total number of 428 (81.99%) of 522 patients were seropositive, 94 (18%) of themwere seronegative. The most frequent rate of seropositivity was among 6-12 (40.89%) ages.The rate was 64.48%, among the children especially between 3 and 12 years of age. Theantibody frequency were growing through 12 years of age, decreased to 7.71%, between 13-17 of ages and among the group of older than 17 years the rate has found as 25.7%. It wasfound that the antibody frequency has changed by age. Defining the reactions against to thedifferent antigene classes has lead to determine the EBV’s serological profiles.The rate of past infection was 50.76% (n=265), the primer infection rate was22.2%(n=116) and the reactivation or chronic infection rate was found as 9%(n=47).Using the EBV serology, may asist to diverse the primary infection, past infection,reactivation and individuals sensitive to EBV. By knowing the epidemiologic factors, thestudies for preventing against the potentially oncocenic virus will become more clear.
Açıklama
Anahtar Kelimeler
Mikrobiyoloji, Tıbbi mikrobiyoloji, Epstein-barr virüs antikorları, Ifa yöntemi, Microbiology, Medical microbiology, Epstein-barr virus antibodies, Performance method
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Gedik, M. (2007). Değişik yaş gruplarında epstein-barr virüs antikorlarının ıfa yöntemiyle araştırılması. Doktora tezi, Dicle Üniversitesi, Diyarbakır.
