HN1 interacts with ?-tubulin to regulate centrosomes in advanced prostate cancer cells

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dc.authorid0000-0002-4337-9327en_US
dc.contributor.authorVarışli, Lokman
dc.contributor.authorJaved, Aadil
dc.contributor.authorÖztürk, Bilge Esin
dc.contributor.authorAkyüz, Gencer Kaan
dc.contributor.authorTakir, Gulevin
dc.contributor.authorFani-Marlen, Roumelioti
dc.contributor.authorGagos, Sarantis
dc.contributor.authorYörükoğlu, Kutsal
dc.contributor.authorKorkmaz, Kemal Sami
dc.date.accessioned2022-08-31T13:40:41Z
dc.date.available2022-08-31T13:40:41Z
dc.date.issued2021en_US
dc.departmentDicle Üniversitesi, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.description.abstractProstate cancer is one of the most common cancer for men worldwide with advanced forms showing supernumerary or clustered centrosomes. Hematological and neurological expressed 1 (HN1) also known as Jupiter Microtubule Associated Homolog 1 (JPT1) belongs to a small poorly understood family of genes that are evolutionarily conserved across vertebrate species. The co-expression network of HN1 from the TCGA PRAD dataset indicates the putative role of HN1 in centrosome-related processes in the context of prostate cancer. HN1 expression is low in normal RWPE-1 cells as compared to cancerous androgen-responsive LNCaP and androgen insensitive PC-3 cells. HN1 overexpression resulted in differential response for cell proliferation and cell cycle changes in RWPE-1, LNCaP, and PC-3 cells. Since HN1 overexpression increased the proliferation rate in PC-3 cells, these cells were used for functional characterization of HN1 in advanced prostate carcinogenesis. Furthermore, alterations in HN expression led to an increase in abnormal to normal nuclei ratio and increased chromosomal aberrations in PC-3 cells. We observed the co-localization of HN1 with γ-tubulin foci in prostate cancer cells, further validated by immunoprecipitation. HN1 was observed as physically associated with γ-tubulin and its depletion led to increased γ-tubulin foci and disruption in microtubule spindle assembly. Higher HN1 expression was correlated with prostate cancer as compared to normal tissues. The restoration of HN1 expression after silencing suggested that it has a role in centrosome clustering, implicating a potential role of HN1 in cell division as well as in prostate carcinogenesis warranting further studies.en_US
dc.identifier.citationVarışli, L., Javed, A., Öztürk, B.E., Takir, G., Roumelioti, F.M. (2021). HN1 interacts with γ-tubulin to regulate centrosomes in advanced prostate cancer cells, Cell Cycle, 20(17), 1724-1744. https://doi.org/10.1080/15384101.2021.1962624en_US
dc.identifier.doi10.1080/15384101.2021.1962624
dc.identifier.endpage1744en_US
dc.identifier.issue17en_US
dc.identifier.startpage1724en_US
dc.identifier.uriwww.tandfonline.com/doi/pdf/10.1080/15384101.2021.1962624?needAccess=true
dc.identifier.urihttps://hdl.handle.net/11468/10309
dc.identifier.volume20en_US
dc.indekslendigikaynakPubMed
dc.institutionauthorVarışli, Lokman
dc.language.isoenen_US
dc.publisherTaylor & Francisen_US
dc.relation.ispartofCell Cycle
dc.relation.publicationcategoryMakale - Uluslararası - Editör Denetimli Dergien_US
dc.relation.tubitak113Z083
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCentrosomeen_US
dc.subjectChromosomeen_US
dc.subjectHN1en_US
dc.subjectProstate canceren_US
dc.subjectSpindle assemblyen_US
dc.titleHN1 interacts with ?-tubulin to regulate centrosomes in advanced prostate cancer cellsen_US
dc.titleHN1 interacts with ?-tubulin to regulate centrosomes in advanced prostate cancer cells
dc.typeArticleen_US

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