Akciğer iskemi reperfüzyon hasarında Melatonin ve N-Asetilsisteinin etkileri
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Tarih
2017
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info:eu-repo/semantics/openAccess
Özet
İ/R hasarı akciğer transplantasyonunda mortalite ve morbitideyi etkileyen önemli etkenlerden bir tanesidir. İ/R hasarı konusunda birçok çalışma yapılmış olup, Melatonin ve NAC `in İ/R hasarında akciğeri koruyucu etkisi olduğu birçok çalışmada gösterilmiştir. Bizim yaptığımız bu çalışmada Melatonin ve/veya NAC akciğer İ/R hasarındaki koruyucu etkisini LPDS eşliğinde göstermeye çalıştık. Çalışmada 48 adet Spraque-downey 300 mgr erkek ratlarda Akciğer İ/R hasarında Melatonin ve/veya NAC `ın etkisini değerlendirmeyi amaçladık. Bu çalışma Dicle Üniversitesi Hayvan Yerel Etik Kurulu tarafından onaylandı ve yaklaşık 8 ay kadar çalışma sürdü. Çalışmada 48 adet rat denek olarak 8 farklı grupta kullanıldı. 1. Grup: sol akciğerde iskemi 2. Grup: sol akciğerde iskemi + M 3. Grup: sol akciğerde iskemi + NAC 4. Grup: sol akciğerde iskemi + M + NAC 5. Grup: sol akciğerde iskemi + reperfüzyon 6. Grup: sol akciğerde iskemi + reperfüzyon + M 7. Grup: sol akciğerde iskemi + reperfüzyon + NAC 8. Grup: sol akciğerde iskemi + reperfüzyon + M + NAC Denekler Na-Pentotal ile uyutuldu. Trakeostomi açılarak entube edildi ve mekanik ventilatöre bağlandı. Tidal volüm 10 ml /kg solunum dk'da 70 olarak ventile edildi. Median sternatomi uygulandı , daha sonra pulmoner arterden heparinize edildi. Pulmoner hilus 2 saat klempe edilerek iskemi sağlandı. Daha sonra klemp kaldırılarak 2 saat reperfüzyon sağlandı. Daha sonra bütün ratlar kalpten yapılan ponksiyon işlemi ile sakrifiye edildi Alınan sol akciğerin 1/3 lük kısmı Işık Mikroskopu altında İ/R hasarı değerlendirildi. Akciğerde İ/R bağlı olarak gelişebilecek alveolar hemoraji ve Akciğerdeki inflamatuar yanıta bağlı gelişebilecek ödem ve inflamasyon değerlendirildi. Geri kalan akciğerde İ/R hasarının göstergesi olabilecek MDA düzeyi bakıldı. Ayrıca ratlardan alınan kanda serum TAOK düzeyi ve sol akciğer BAL sıvısında Nötrofil yüzdesine bakıldı.
İschemia-reperfusion injury is one of the important factors effecting on mortality and morbidity in lung transplantation.There are many studies about İ/R injury and these studies showed that Melatonin and NAC have protective effect on lung against İ/R injury . In the present study, we have tried to show the protective effect of Melatonin and NAC together LPDS on lung İ/R injury Our study was performed on 48 male Sprague-downey rats weighting 300 g . We aimed to evaluate the effect of Melatonin and NAC on lung İ/R injury in these rats. This study was approved by Dicle Üniversity ( Animal Local Ethical Committee ) and it has continued eight months A total of 48 rats were used as samples in 8 different groups : 1. group : left lung ischemia 2. group : left lung ischemia + Melatonin 3. group : left lung ischemia + NAC 4. group : left lung ischemia + Melatonin + NAC 5. group : left lung ischemia + reperfusion 6. group : left lung ischemia + reperfusion + Melatonin 7. group : left lung ischemia + reperfusion + NAC 8. group : left lung ischemia + reperfusion + Melatonin + NAC Samples were anesthetized with Na-Pentotal. They were intubated through a tracheostomy and connected to mechanic ventilator. They were ventilated with a tidal volume of 10 ml/kg at 70 breaths / min. Median sternatomy was performed after this heparin was injected into pulmonary artery. The hilum of pulmonary was clamped to keep ischemia during 2 hours. Then clamp was removed for 2 hours reperfusion 1/3 of the left donor lung was assessed for İ/R injury under the Light Microscope. It was evaluated alveolary hemoragia which depends on İ/R injury in the lung and odema and inflamation that depend on the inflamation response of the lung. It was determined the level of MDA which may be a semptom of İ/R injury in the rest of the lung Additionally, it was assessed the level of seruma TAOK in blood of rats and the rate of neutrofil in left lung BAL.
İschemia-reperfusion injury is one of the important factors effecting on mortality and morbidity in lung transplantation.There are many studies about İ/R injury and these studies showed that Melatonin and NAC have protective effect on lung against İ/R injury . In the present study, we have tried to show the protective effect of Melatonin and NAC together LPDS on lung İ/R injury Our study was performed on 48 male Sprague-downey rats weighting 300 g . We aimed to evaluate the effect of Melatonin and NAC on lung İ/R injury in these rats. This study was approved by Dicle Üniversity ( Animal Local Ethical Committee ) and it has continued eight months A total of 48 rats were used as samples in 8 different groups : 1. group : left lung ischemia 2. group : left lung ischemia + Melatonin 3. group : left lung ischemia + NAC 4. group : left lung ischemia + Melatonin + NAC 5. group : left lung ischemia + reperfusion 6. group : left lung ischemia + reperfusion + Melatonin 7. group : left lung ischemia + reperfusion + NAC 8. group : left lung ischemia + reperfusion + Melatonin + NAC Samples were anesthetized with Na-Pentotal. They were intubated through a tracheostomy and connected to mechanic ventilator. They were ventilated with a tidal volume of 10 ml/kg at 70 breaths / min. Median sternatomy was performed after this heparin was injected into pulmonary artery. The hilum of pulmonary was clamped to keep ischemia during 2 hours. Then clamp was removed for 2 hours reperfusion 1/3 of the left donor lung was assessed for İ/R injury under the Light Microscope. It was evaluated alveolary hemoragia which depends on İ/R injury in the lung and odema and inflamation that depend on the inflamation response of the lung. It was determined the level of MDA which may be a semptom of İ/R injury in the rest of the lung Additionally, it was assessed the level of seruma TAOK in blood of rats and the rate of neutrofil in left lung BAL.
Açıklama
Anahtar Kelimeler
Akciğer, Lung, Akciğer hastalıkları, Lung diseases, Melatonin, N-asetilsistein, N-acetylcysteine, Reperfüzyon, Reperfusion, Reperfüzyon lezyonu, Reperfusion injury, İskemi, Ischemia
