PURIFICATION OF THERMOSTABLE ?-GALACTOSIDASE FROM ANOXYBACILLUS SP KP1 AND ESTIMATION OF COMBINED EFFECT OF SOME CHEMICALS ON ENZYME ACTIVITY USING SEMIPARAMETRIC ERRORS IN VARIABLES MODEL

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dc.contributor.authorBekler, Fatma Matpan
dc.contributor.authorYalaz, Secil
dc.contributor.authorAcer, Omer
dc.contributor.authorGuven, Kemal
dc.date.accessioned2024-04-24T17:40:02Z
dc.date.available2024-04-24T17:40:02Z
dc.date.issued2017
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe thermostable beta-galactosidase from thermophilic Anoxybacillus sp. KP1 was partially purified by Sephadex G-75 gel permeation chromatography with a fold purification of 14.4 and a yield of 11.8%, respectively. The optimal activity of purified beta-galactosidase was pH 9.0 and a temperature of 60 degrees C. The enzyme was stable at pH 9.0. Thermostability of the enzyme was also 60 degrees C. The molecular weight of the enzyme was determined as 68 kDa by SDS-PAGE. The partially purified thermostable alkaline beta-galactosidase was significantly inhibited by various divalent cations, including Hg2+ and Cu2+ while Ca2+ and Mg2+ ions activated the enzyme activity. The combined effects of some chemicals on beta-galactosidase activity by Anoxybacillus sp. KP1 were studied using semiparametric errors in variables methodology. The optimal combinations of chemicals concen-tration for maximum beta-galactosidase activity was determined as 4 mM DTT, 4 mM PMSF, 4 mM NEM, 4 mM Iod A, 4 mM beta-Mer and 10 mM phen.en_US
dc.identifier.endpage2261en_US
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issue3en_US
dc.identifier.startpage2253en_US
dc.identifier.urihttps://hdl.handle.net/11468/21546
dc.identifier.volume26en_US
dc.identifier.wosWOS:000399966600050
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.language.isoenen_US
dc.publisherParlar Scientific Publications (P S P)en_US
dc.relation.ispartofFresenius Environmental Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBeta-Galactosidaseen_US
dc.subjectCharacterizationen_US
dc.subjectInhibitoren_US
dc.subjectPurificationen_US
dc.subjectSemiparametric Eiv Modelen_US
dc.titlePURIFICATION OF THERMOSTABLE ?-GALACTOSIDASE FROM ANOXYBACILLUS SP KP1 AND ESTIMATION OF COMBINED EFFECT OF SOME CHEMICALS ON ENZYME ACTIVITY USING SEMIPARAMETRIC ERRORS IN VARIABLES MODELen_US
dc.titlePURIFICATION OF THERMOSTABLE ?-GALACTOSIDASE FROM ANOXYBACILLUS SP KP1 AND ESTIMATION OF COMBINED EFFECT OF SOME CHEMICALS ON ENZYME ACTIVITY USING SEMIPARAMETRIC ERRORS IN VARIABLES MODEL
dc.typeArticleen_US

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