An epidemiological study of Entamoeba histolytica and Entamoeba dispar infection using polymerase chain reaction

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dc.contributor.authorGoral V.
dc.contributor.authorJetter A.
dc.contributor.authorWalderish B.
dc.contributor.authorBurchard G.-D.
dc.contributor.authorAckers J.
dc.contributor.authorBritten D.
dc.date.accessioned2024-04-24T17:58:51Z
dc.date.available2024-04-24T17:58:51Z
dc.date.issued1998
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe recent discovery of the existence of Entamoeba dispar (E.dispar) as an amoeba that is constitutively nonpathogenic but morphologically indistinguishable from Entamoeba histolytica (E.histolytica), has raised the question of the validity of most epidemiological studies on amoebiasis. This study was conducted in two villages. The presence of amoeba was determined by microscopic examination of fresh stools and formalin-ether-concentrated specimens. An improved colorimetric 'polymerase chain reaction' (PCR) method was used and ELISA assay was used to detect anti-E.histolytica antibodies in the serum samples. The combined frequencies of E.histolytica/E.dispar according to stool microscopy were 35% in village A, and 28% in village B. According to PCR results, E.histolytica was not detected and E.dispar was found in 21% and 18% of stool samples in A and B villages respectively. Thirty-five percent (92/260) of the sera reacted with E.histolytica antigen and 28% (72/260) of the sera reacted with E.dispar antigen. A correlation between antibody levels and the presence of E.histolytica/E.dispar in stool microscopy could not be detected. A significant correlation was found between antibody responses to E.dispar and E.histolytica antigen (p=0.001). According to our results, the prevalence of E.histolytica in our area seems to be very low. Serology is not helpful in distinguishing E.histolytica from E.dispar in asymptomatic cyst passers in an endemic area.en_US
dc.identifier.endpage376en_US
dc.identifier.issn1300-4948
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-0032438719en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage374en_US
dc.identifier.urihttps://hdl.handle.net/11468/24131
dc.identifier.volume9en_US
dc.indekslendigikaynakScopus
dc.language.isotren_US
dc.relation.ispartofTurkish Journal of Gastroenterologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectColorimetric Pcren_US
dc.subjectEntamoeba Disparen_US
dc.subjectEntamoeba Histolyticaen_US
dc.subjectEpidemiogyen_US
dc.subjectTanien_US
dc.titleAn epidemiological study of Entamoeba histolytica and Entamoeba dispar infection using polymerase chain reactionen_US
dc.title.alternativePolymerase chain reaction analizi ile Entamoeba histolytica ve Entamoeba dispar sikligi (epidemiyolojik calisma)en_US
dc.typeArticleen_US

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