Production and Optimization of Exopolysaccharide from Thermophilic Bacteria

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dc.contributor.authorGüven, Kemal
dc.contributor.authorBekler, Fatma Matpan
dc.contributor.authorPolat, Nazlı
dc.contributor.authorTuşar, Firdevs Rozan
dc.date.accessioned2024-04-24T19:13:14Z
dc.date.available2024-04-24T19:13:14Z
dc.date.issued2022
dc.departmentDicle Üniversitesien_US
dc.description.abstractExopolysaccharides (EPS) are the large molecular weight carbohydrate polymers extracted from higher plants, algae, fungi and bacteria. The thermophilic Bacillus zhangzhounesis 2CA and Bacillus licheniformis 2CS used in the present study were isolated from Çermik hot springs. The growth conditions of the strains designated as 2CA and 2CS in different basal media (M1, M2 and M3), different carbon sources and different concentrations of yeast extract (%w v-1: 0.05, 0.1, 0.15 and 0.2) and the amount of EPS produced were investigated. In addition, the phenol-sulfuric acid method and the Lowry method were used to determine the amount of carbohydrates and proteins within the EPS produced by the bacteria, respectively. The highest total EPS dry weight for B. licheniformis 2ÇS was obtained as 121 mg in M3 medium (0.2% yeast extract + 1% sucrose), carbohydrate content in EPS was 333.28 µg mL-1 and protein content was 0.19 µg mL-1. When these two bacteria were compared in terms of the amount of carbohydrates in the EPS produced, the highest amount of carbohydrates was found in EPS of B. zhangzhounesis 2CA (1087.03 µg mL-1). The antibacterial effects of EPS were investigated against pathogenic microorganisms (E. coli, S. aureus, K. pneumoniae and P. aeruginosa). It was determined that the highest antibacterial activity against E. coli (with 16 mm zone diameter) was obtained with EPS produced by B. licheniformis 2ÇS bacteria in M3 medium (0.2% yeast extract + 1% sucrose).en_US
dc.identifier.doi10.29132/ijpas.1142315
dc.identifier.endpage533en_US
dc.identifier.issn2149-0910
dc.identifier.issue2en_US
dc.identifier.startpage524en_US
dc.identifier.trdizinid1151572
dc.identifier.urihttps://doi.org/10.29132/ijpas.1142315
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1151572
dc.identifier.urihttps://hdl.handle.net/11468/28479
dc.identifier.volume8en_US
dc.indekslendigikaynakTR-Dizin
dc.language.isoenen_US
dc.relation.ispartofInternational Journal of Pure and Applied Sciences
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleProduction and Optimization of Exopolysaccharide from Thermophilic Bacteriaen_US
dc.titleProduction and Optimization of Exopolysaccharide from Thermophilic Bacteria
dc.typeArticleen_US

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