PCR-based detection of alkane monooxygenase genes in the hydrocarbon and crude oil-degrading Acinetobacter strains from petroleum-contaminated soils

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dc.contributor.authorEren, Ayşe
dc.contributor.authorBekler, Fatma Matpan
dc.contributor.authorGüven, Kemal
dc.date.accessioned2025-02-22T14:10:55Z
dc.date.available2025-02-22T14:10:55Z
dc.date.issued2024
dc.departmentDicle Üniversitesien_US
dc.description.abstractBacterial strains D11, E1 and E2 isolated from petroleum-contaminated soils were found to be members of Acinetobacter genus revealed by 16S rRNA gene sequence analysis and phenotypic characteristics. After incubation for 5 days, about 43, 9 and 12 % of total petroleum hydrocarbons of crude oil were degraded by strains D11, E1 and E2, respectively, and determined by GC-MS analysis. Moreover, about 70 and 76 % of single hydrocarbon hexadecane was degraded by the strains D11 and E1 after 3 days of short incubation time, respectively, while the strain E2 degraded about 48 % of single hydrocarbon pentadecane. By using PCR-based method, gene sequences of the strains D11 and E2 showed similarity to alkane 1-monooxygenases from Acinetobacter sp. BUU8 alkM with 93.06 and 92.72 %, respectively, while the sequence similarity of strain E1 was 95.84 % to Acinetobacter sp. 826659. The present study of hydrocarbon biodegradation by Acinetobacter strains may provide a good advantage in bioremediation process. © 2024 Physical Society of Japan. All rights reserved.en_US
dc.identifier.doi10.2298/JSC230707053E
dc.identifier.endpage334en_US
dc.identifier.issn0352-5139
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85191948675en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage321en_US
dc.identifier.urihttps://doi.org/10.2298/JSC230707053E
dc.identifier.urihttps://hdl.handle.net/11468/29896
dc.identifier.volume89en_US
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherSerbian Chemical Societyen_US
dc.relation.ispartofJournal of the Serbian Chemical Societyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKA_Scopus_20250222
dc.subjectalkM geneen_US
dc.subjectbacteriaen_US
dc.subjectGC-MS analysisen_US
dc.subjectpetroleum-degradationen_US
dc.titlePCR-based detection of alkane monooxygenase genes in the hydrocarbon and crude oil-degrading Acinetobacter strains from petroleum-contaminated soilsen_US
dc.typeArticleen_US

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