Prokaryotlarda proteolitik aktivite
Yükleniyor...
Tarih
2018
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada Çermik Termal kaplıcalarından izole edilerek tür teşhisi yapılan termotoleran Bacillus subtilis kullanıldı. B.subtilis'in hangi besi ortamında daha iyi Nötral proteaz ürettiği saptanarak bu ortamdan nötral proteazın saflaştırmasına gidildi. Yapılan deneylerde 10~5 M Zn2+ içeren nutrient broth besiyerinde üretilen B. subtilis'in salgıladığı nötral proteazın hem aktivite yönünden, hem de salgılanan enzim miktarı bakımından en uygun besi ortamı olduğu saptandı.Saf olarak elde edilen Nötral proteaz için molekül ağırlığı 29.000 Dalton, Km değeri de 0.952 olarak belirlendi.
In this study, thermotoleran Bacillus subtilis which isolated from Çermik thermal hot spring and identified has been used. The most appropriate growth medium in producing of the neutral protease was determined and the neutral protease was purified from this medium. With the experiments have been done, neutral protease of B.subtilis that produced in nutrient broth which contains 10"5 M Zn2+, both activity and amount of secreted enzyme has been obtained as the best growth medium. It was counted that molecular weight of the purified neutral protease was 29.000 Dalton and for Km was 0.952.
In this study, thermotoleran Bacillus subtilis which isolated from Çermik thermal hot spring and identified has been used. The most appropriate growth medium in producing of the neutral protease was determined and the neutral protease was purified from this medium. With the experiments have been done, neutral protease of B.subtilis that produced in nutrient broth which contains 10"5 M Zn2+, both activity and amount of secreted enzyme has been obtained as the best growth medium. It was counted that molecular weight of the purified neutral protease was 29.000 Dalton and for Km was 0.952.
Açıklama
Anahtar Kelimeler
Bacillus subtilis, Biyoteknoloji, Biotechnology, Mikroorganizmalar, Microorganisms, Saflaştırma, Purification
