Effects of Gallic Acid on Ischemia-Reperfusion Induced Testicular Injury in a Rat Testicular Torsion Model

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dc.contributor.authorSogutcu, Nilgun
dc.contributor.authorYokus, Beran
dc.date.accessioned2024-04-24T17:47:52Z
dc.date.available2024-04-24T17:47:52Z
dc.date.issued2021
dc.departmentDicle Üniversitesien_US
dc.description.abstractOBJECTIVE: Testicular torsion is a common urological complication mostly affecting adolescents and young men. In this study we investigated biochemical and immunohistochemical effects of gallic acid on the damage induced by testicular torsion-detorsion. STUDY DESIGN: Forty male rats were divided into 4 groups of 10 animals each: control, torsion, torsion/detorsion, and torsion/detorsion + gallic acid. Testicles were removed from the scrotum, and 2.5-hour ischemia was applied to the left testis by twisting the spermatic cord 720 degrees clockwise. Then 3-hour reperfusion was allowed for detorsion. Gallic acid 20 mg/kg was orally administered to the torsion/detorsion + gallic acid group before reperfusion. Biochemical parameters of testicular tissue (MDA, SOD, CAT, and GSH levels) were measured. Testicular tissues were immune-stained with caspase-3 and TNF-alpha antibody. RESULTS: MDA levels in the torsion/detorsion + gallic acid group were close to those in the control group; however, it was higher in the torsion/detorsion group as compared to the control group. Compared to control group, SOD, CAT, and GSH activities were significantly increased in the torsion/detorsion + gallic acid group. However, those values were decreased in the torsion-detorsion group. Spermatogenic cells and interstitial cells showed positive caspase-3 expression in the torsion and torsion-detorsion groups; however, expression level was decreased in the torsion/detorsion + gallic acid group. TNF-alpha expression was observed in degenerated spermatogonia, Leydig cells, and macrophages in the torsion and torsion-detorsion groups. In the torsion/detorsion + gallic acid group, TNF-alpha expression was observed in some interstitial region rather than in cells of seminiferous tubules. CONCLUSION: Gallic acid treatment could be an alternative therapy in testicular ischemia-reperfusion injury to decrease inflammation, germ cell degeneration, and apoptosis.en_US
dc.identifier.endpage7en_US
dc.identifier.issn0884-6812
dc.identifier.issue1en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://hdl.handle.net/11468/22790
dc.identifier.volume43en_US
dc.identifier.wosWOS:000640456900001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.language.isoenen_US
dc.publisherSci Printers & Publ Incen_US
dc.relation.ispartofAnalytical and Quantitative Cytopathology and Histopathology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDetorsionen_US
dc.subjectIschemia-Reperfusion Injuryen_US
dc.subjectOxidative Stressen_US
dc.subjectRatsen_US
dc.subjectWistaren_US
dc.subjectReperfusion Injuryen_US
dc.subjectSpermatic Cord Torsionen_US
dc.subjectSpermatogenesisen_US
dc.subjectTesticular Torsionen_US
dc.subjectTestisen_US
dc.subjectTorsion Abnormalityen_US
dc.subjectTorsion-Detorsionen_US
dc.titleEffects of Gallic Acid on Ischemia-Reperfusion Induced Testicular Injury in a Rat Testicular Torsion Modelen_US
dc.titleEffects of Gallic Acid on Ischemia-Reperfusion Induced Testicular Injury in a Rat Testicular Torsion Model
dc.typeArticleen_US

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