Effects of Gallic Acid on Ischemia-Reperfusion Induced Testicular Injury in a Rat Testicular Torsion Model
dc.contributor.author | Sogutcu, Nilgun | |
dc.contributor.author | Yokus, Beran | |
dc.date.accessioned | 2024-04-24T17:47:52Z | |
dc.date.available | 2024-04-24T17:47:52Z | |
dc.date.issued | 2021 | |
dc.department | Dicle Üniversitesi | en_US |
dc.description.abstract | OBJECTIVE: Testicular torsion is a common urological complication mostly affecting adolescents and young men. In this study we investigated biochemical and immunohistochemical effects of gallic acid on the damage induced by testicular torsion-detorsion. STUDY DESIGN: Forty male rats were divided into 4 groups of 10 animals each: control, torsion, torsion/detorsion, and torsion/detorsion + gallic acid. Testicles were removed from the scrotum, and 2.5-hour ischemia was applied to the left testis by twisting the spermatic cord 720 degrees clockwise. Then 3-hour reperfusion was allowed for detorsion. Gallic acid 20 mg/kg was orally administered to the torsion/detorsion + gallic acid group before reperfusion. Biochemical parameters of testicular tissue (MDA, SOD, CAT, and GSH levels) were measured. Testicular tissues were immune-stained with caspase-3 and TNF-alpha antibody. RESULTS: MDA levels in the torsion/detorsion + gallic acid group were close to those in the control group; however, it was higher in the torsion/detorsion group as compared to the control group. Compared to control group, SOD, CAT, and GSH activities were significantly increased in the torsion/detorsion + gallic acid group. However, those values were decreased in the torsion-detorsion group. Spermatogenic cells and interstitial cells showed positive caspase-3 expression in the torsion and torsion-detorsion groups; however, expression level was decreased in the torsion/detorsion + gallic acid group. TNF-alpha expression was observed in degenerated spermatogonia, Leydig cells, and macrophages in the torsion and torsion-detorsion groups. In the torsion/detorsion + gallic acid group, TNF-alpha expression was observed in some interstitial region rather than in cells of seminiferous tubules. CONCLUSION: Gallic acid treatment could be an alternative therapy in testicular ischemia-reperfusion injury to decrease inflammation, germ cell degeneration, and apoptosis. | en_US |
dc.identifier.endpage | 7 | en_US |
dc.identifier.issn | 0884-6812 | |
dc.identifier.issue | 1 | en_US |
dc.identifier.startpage | 1 | en_US |
dc.identifier.uri | https://hdl.handle.net/11468/22790 | |
dc.identifier.volume | 43 | en_US |
dc.identifier.wos | WOS:000640456900001 | |
dc.identifier.wosquality | Q4 | |
dc.indekslendigikaynak | Web of Science | |
dc.language.iso | en | en_US |
dc.publisher | Sci Printers & Publ Inc | en_US |
dc.relation.ispartof | Analytical and Quantitative Cytopathology and Histopathology | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Detorsion | en_US |
dc.subject | Ischemia-Reperfusion Injury | en_US |
dc.subject | Oxidative Stress | en_US |
dc.subject | Rats | en_US |
dc.subject | Wistar | en_US |
dc.subject | Reperfusion Injury | en_US |
dc.subject | Spermatic Cord Torsion | en_US |
dc.subject | Spermatogenesis | en_US |
dc.subject | Testicular Torsion | en_US |
dc.subject | Testis | en_US |
dc.subject | Torsion Abnormality | en_US |
dc.subject | Torsion-Detorsion | en_US |
dc.title | Effects of Gallic Acid on Ischemia-Reperfusion Induced Testicular Injury in a Rat Testicular Torsion Model | en_US |
dc.title | Effects of Gallic Acid on Ischemia-Reperfusion Induced Testicular Injury in a Rat Testicular Torsion Model | |
dc.type | Article | en_US |