?-AMYLASE FROM BACILLUS SIMPLEX- PRODUCTION, CHARACTERIZATION AND PARTIAL PURIFICATION

Basit Öğe Kaydı
dc.contributor.authorOrtakaya, Veysi
dc.contributor.authorFincan, Sema Aguloglu
dc.contributor.authorEnez, Baris
dc.date.accessioned2024-04-24T17:47:29Z
dc.date.available2024-04-24T17:47:29Z
dc.date.issued2017
dc.departmentDicle Üniversitesien_US
dc.description.abstractIn this study, some bacteria were isolated from the soil obtained from Ergani Makam Mountain. The isolated bacteria were defined as Bacillus simplex with the biochemical tests and 16S rRNA analysis. The optimum conditions for bacterial growth were determined as the 32th hour, 37 degrees C and pH 7.0. While the maximum amylase activity was observed of the 72nd hour, the optimum temperature and pH of the enzyme were determined to be 37 degrees C and 7.0 reciprocally. It was observed that the enzyme production increased with ammonium sulfate and ammonium nitrate from nitrogen sources and decreased with the addition of carbon sources. Amylase was partially purified with 70% ammonium sulfate precipitations and dialysis. When the effect of detergents was examined on the partially purified enzyme it was determined that SDS considerably inhibited and the other detergents increased the enzyme activity. It was also determined that while CaCl2 increased the activity whereas FeCl2, CuCl2, ZnCl2 and HgCl2 considerably inhibited the enzyme activity.en_US
dc.identifier.endpage4455en_US
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issue7en_US
dc.identifier.startpage4446en_US
dc.identifier.urihttps://hdl.handle.net/11468/22541
dc.identifier.volume26en_US
dc.identifier.wosWOS:000406355800016
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.language.isoenen_US
dc.publisherParlar Scientific Publications (P S P)en_US
dc.relation.ispartofFresenius Environmental Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBacillus Simplexen_US
dc.subjectAlpha-Amylaseen_US
dc.subjectIsolationen_US
dc.subjectProductionen_US
dc.subjectPartial Purificationen_US
dc.subjectCharacterizationen_US
dc.title?-AMYLASE FROM BACILLUS SIMPLEX- PRODUCTION, CHARACTERIZATION AND PARTIAL PURIFICATIONen_US
dc.title?-AMYLASE FROM BACILLUS SIMPLEX- PRODUCTION, CHARACTERIZATION AND PARTIAL PURIFICATION
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu