İneklerde gebelik süresince plasentada bazı homeobox (HOX) proteinlerinin dağılımı
Yükleniyor...
Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Dicle Üniversitesi, Sağlık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bu çalışma, gebelik süresince inek uterus ve plasentasında bazı Homeobox proteinlerinin (HOXA10, HOXA11, HOXB6, HOXC6, TLX1, Dlx-5, HLX) dağılımlarını tespit etmek amacıyla tasarlanmıştır. Gereç ve Yöntem: Araştırmada Diyarbakır ve Hatay'da bulunun özel kesimhanelerden elde edilen toplam 27 adet Holstein ırkı gebe inek uterus ve plasentası kullanıldı. Kullanılan materyalin gebeliğin hangi dönemine ait olduğunu saptamak için fötusların alın sağrı uzunluğu ölçülerek fötusların yaşları belirlendi. Böylece belirlenen fötus yaşlarına dayanarak dokular gebeliğin ilk, orta ve son dönemine ait olacak şekilde üç gruba ayrıldı. Kullanılan bütün doku örnekleri %10'luk nötral formalin solüsyonunda 24 saat boyunca tespit edildi ve daha sonra dokular rutin histolojik aşamalardan geçirilerek parafinde bloklandı. Hazırlanan parafin bloklarından, 5 mikrometre kalınlığında seri kesitler alındı ve alınan kesitler çalışmada belirlenen genlerle uygun antikorlar kullanılarak immunohistokimya boyamasına tabi tutuldu. Bulgular: Bu araştırmadan elde edilen immunohistokimyasal bulguların değerlendirilmesi sonucunda, gebelik sürecindeki dönemlere bağlı olarak uterus ve plasentada değişik yoğunluklarda immunreaksiyonun görüldüğü tespit edildi. İmmunreaksiyon özellikle, uterusun luminal ve glanduler epitel hücreleri ile stromal ve bazı endotel hücrelerde gözlenmesinin yanı sıra HOXA10, HOXB6, HOXC6 ve Dlx-5 genlerinin düz kas hücrelerinde de reaksiyon verdiği belirlendi. Bunlarla birlikte, plasentanın yapısına katılan maternal epitel ve fötal trofoblastlarda da immunoreaktivitenin olduğu saptandı. Sonuç: Sonuç olarak; bazı Homeobox proteinlerin, endometriyal fonksiyonların düzenlenmesinde, endometriyal ve plasental hücrelerin çoğalıp farklılaşmasında kritik rollere sahip olabileceği düşünüldü. Bunun yanı sıra plasenta oluşumu ve gelişiminde olduğu gibi gebeliğin sürdürülmesinde de fizyolojik rollere sahip olabilecekleri anlaşıldı.
Aim: This study was designed to determine the distribution of some Homeobox proteins (HOXA10, HOXA11, HOXB6, HOXC6, TLX1, Dlx-5, HLX) in the cow uterus and placenta during pregnancy. Material and Method: A total of 27 Holstein breed pregnant cows uterus and placenta obtained from private slaughterhouses in Diyarbakır and Hatay were used in the study. The age of the fetuses was determined by measuring the frontal rump length of the fetuses in order to determine the term of pregnancy of the material used. Based on the determined fetus age, the tissues were divided into three groups, belonging to the first, middle and last period of pregnancy. All tissue samples used were fixed in a 10% neutral formalin solution for 24 hours and then the tissues were passed through routine histological stages and blocked in paraffin. Serial sections 5 micrometers thick were taken from the prepared paraffin blocks and the sections were subjected to immunohistochemical staining using appropriate antibodies related to the genes indicated in the study. Results: As a result of the evaluation of the immunohistochemical findings obtained from this research, it was determined that different intensities of immunreactivity were observed in the uterus and placenta, depending on the periods during pregnancy. In particular, the immunreaction was observed in the luminal and glandular epithelial cells, stromal and some endothelial cells of the uterus, as well as the HOXA10, HOXB6, HOXC6 and Dlx-5 genes were localized in smooth muscle cells. In addition to these, immunoreactivity was also found in maternal epithelial and fötal trophoblasts involved in the structure of the placenta. Conclusion: As a result, it was thought that some Homeobox proteins may have critical roles in the regulation of endometrial functions, in the proliferation and differentiation of endometrial and placental cells. In addition, it was understood that they may have physiological roles in maintaining pregnancy as well as placenta formation and development.
Aim: This study was designed to determine the distribution of some Homeobox proteins (HOXA10, HOXA11, HOXB6, HOXC6, TLX1, Dlx-5, HLX) in the cow uterus and placenta during pregnancy. Material and Method: A total of 27 Holstein breed pregnant cows uterus and placenta obtained from private slaughterhouses in Diyarbakır and Hatay were used in the study. The age of the fetuses was determined by measuring the frontal rump length of the fetuses in order to determine the term of pregnancy of the material used. Based on the determined fetus age, the tissues were divided into three groups, belonging to the first, middle and last period of pregnancy. All tissue samples used were fixed in a 10% neutral formalin solution for 24 hours and then the tissues were passed through routine histological stages and blocked in paraffin. Serial sections 5 micrometers thick were taken from the prepared paraffin blocks and the sections were subjected to immunohistochemical staining using appropriate antibodies related to the genes indicated in the study. Results: As a result of the evaluation of the immunohistochemical findings obtained from this research, it was determined that different intensities of immunreactivity were observed in the uterus and placenta, depending on the periods during pregnancy. In particular, the immunreaction was observed in the luminal and glandular epithelial cells, stromal and some endothelial cells of the uterus, as well as the HOXA10, HOXB6, HOXC6 and Dlx-5 genes were localized in smooth muscle cells. In addition to these, immunoreactivity was also found in maternal epithelial and fötal trophoblasts involved in the structure of the placenta. Conclusion: As a result, it was thought that some Homeobox proteins may have critical roles in the regulation of endometrial functions, in the proliferation and differentiation of endometrial and placental cells. In addition, it was understood that they may have physiological roles in maintaining pregnancy as well as placenta formation and development.
Açıklama
Anahtar Kelimeler
Gebe uterus, Homeobox, İnek, Plasenta, Trofoblast, Cow, Placenta, Pregnant uterus, Trophoblast
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Topaloğlu, U. (2020). İneklerde gebelik süresince plasentada bazı homeobox (HOX) proteinlerinin dağılımı. Doktora tezi, Dicle Üniversitesi, Diyarbakır.
