Romatoid artritli hastalarda HLA-DRB1 allellerinin ve DRB1 *01,*04,*07,*08 ve *10 alt tiplerinin analizi
[ X ]
Tarih
2015
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Giriş ve Amaç: Romatoid Artrit (RA) kompleks genetik komponente sahip kronik inflamatuvar bir hastalıktır. Değişik toplumlarda RA ve HLA ilişkisi değerlendirildiği halde Türkiye populasyonuyla ilgili fazla çalışma yapılmamıştır. RA'lı hastalardan bu çalışmada HLA-DRB1 ve bazı alt gruplarının (HLA-DRB1*01, *04, *07, *08, *10) sıklığı, bunların hastalık fonsiyonel evresi ve seropozitivitesi ile olan ilişkisinin belirlenmesi amaçlandı. Materyal ve Metod: Bu çalışmaya, Diyarbakır Dicle Üniversitesi Tıp Fakültesi Fizik Tedavi ve Rehabilitasyon polikliniğine başvuran ve 1987 American Collage of Rheumatology (ACR) kriterlerine göre kesin RA tanısı alan, yaş ortalaması 46.97 ±13.44 olan 96 (78 kadın, 18 erkek) hasta ve yaş ortalaması 29.77± 16.99 olan 84 (36 erkek, 48 kadın ) sağlıklı birey alındı. Çalışmaya dahil edilen bireyler klinik ve laboratuar parametreleri açısından değerlendirildi. HLA-DRB1 genotiplemesi PCR-SSP HLA-DRB1 Low Resolution gen paneli kullanılarak belirlendi. HLADRB1*01, *04, *07, *08, *10'un alt tipleri PCR-SSP HLA-DRB1 High Resolution gen paneli kullanılarak tespit edildi. RA hastalarının fonksiyonel evresi ve seropozitiviteleri değerlendirildi. Bulgular: RA hasta grubunda allel frekansı en fazla olan tipler sırasıyla DRB1*04 (%27.6), DRB1*11 (%14.6) ve DRB1*15 (%13.5) olarak bulunurken; kontrol grubunda ise sırasıyla DRB1*11 (%24.4), DRB1*04 (%19.6) ve DRB1*07 (%14.9) idi. RA hasta grubu ile kontrol grubu arasında allel frekansları karşılaştırıldığında; RA'lı hastalarda DRB1*10 allelinin frekansı anlamlı yüksek iken, DRB1*07 ve DRB1*11 allelleri anlamlı düşük bulundu (p<0.05). Hasta ve kontrol gruplarında HLADRB1 alt tipleri arasında yapılan karşılaştırmada; RA'lı hastalarda DRB1*0401, *0408 ve *1001 alt tiplerinin frekansı anlamlı artmış bulunurken (p<0.05); DRB1*0402, *0403 ve *0701 alt tipleri ise anlamlı düşük bulundu (p<0.05). Seropozitivite ile allel ve alt tipler arasında istatistiksel olarak anlamlı bir ilişki saptanmadı (p>0.05). Fonksiyonel evre ile allel ve alt tipler arasındaki ilişki değerlendirildiğinde; DRB1*10 alleli iyi işlevsel durumla (evre 1-2) ilişkiliyken, DRB1*11 alleli kötü işlevsel durumla (evre 3-4) ilişkili bulundu (p<0.05). Sonuç: Bölgemizdeki RA hastalarında DRB1*0401, *0408, *10 ve *1001 allelleri hastalığa yatkınlıkla ilişkili iken; DRB1*0402, *0403,*0701 ve *11 allelleri ise hastalığa yatkınlığı azaltan koruyucu alleller olarak değerlendirildi. DRB1*10 ve DRB1*11 allelleri hastalık şiddeti üzerine etkili olabilir.
Introduction and Aim: Rheumatoid arthritis (RA) is a complex disorder that has a genetic component. Although associations of RA and HLA have previously been investigated in various populations studies in Turkish population are very limited. Determining the frequency of HLA-DRB1 and its some sub-groups (HLA-DRB1*01, *04, *07, *08, *10) in patients with RA, and their associations with the functional stage and seropositivity of the disease have been aimed in this study. Material and Methods: Among the admissons to Dicle University School of Medicine Physical Therapy and Rehabilitation Outpatient Clinic a total of 96 (78 female, 18 male) patients who were diagnosed with RA according to the 1987 American Collage of Rheumatology (ACR) criteria with the mean age of 46.97 ±13.44 years and 84 (36 male, 48 female) healthy control subjects with the mean age of 29.77± 16.99 years have been introduced to the study. All participants were evaluated in terms of clinical and laboratory parameters. HLA-DRB1 genotyping have been carried up by using PCR-SSP HLA-DRB1 Low Resolution gene panel. Subtypes of HLADRB1*01, *04, *07, *08, *10 were identified by PCR-SSP HLA-DRB1 High Resolution gene panel. Functionality and seropositivity of RA patients were also evaluated. Results: In RA patients types which have highest allel frequencies are DRB1*04 (%27.6), DRB1*11 (%14.6), and DRB1*15 (%13.5) in order; whereas, it was DRB1*11 (%24.4), DRB1*04 (%19.6), and DRB1*07 (%14.9) in the control group respectively. When allel frequecies of RA patients and control subjects were compared, the frequency of DRB1*10 allel was found to be higher, and DRB1*07 ve DRB1*11 allel frequencies were found to be significantly lower in RA patients (p<0.05). The comparison of HLADRB1 sub-types between groups showed significantly higher frequencies of DRB1*0401, *0408, and *1001 sub-types (p<0.05), and significantly lower frequencies of DRB1*0402, *0403, and *0701 sub-types in RA patients (p<0.05). There were no statistically significant association between seropositivity and allels and their sub-types (p>0.05). DRB1*10 allel was found to be associated with good functional status (stage 1-2); whereas, DRB1*11 was with poor functional status (stage 3-4) (p<0.05). Conclusions: In RA patients living in our area, DRB1*0401, *0408, *10, and *1001 allels might be associated with increased tendency to the disease; whereas, DRB1*0402, *0403,*0701, and *11 allels with decreased tendency to it. DRB1*10 and DRB1*11 allels may be related with disease severity in RA patients.
Introduction and Aim: Rheumatoid arthritis (RA) is a complex disorder that has a genetic component. Although associations of RA and HLA have previously been investigated in various populations studies in Turkish population are very limited. Determining the frequency of HLA-DRB1 and its some sub-groups (HLA-DRB1*01, *04, *07, *08, *10) in patients with RA, and their associations with the functional stage and seropositivity of the disease have been aimed in this study. Material and Methods: Among the admissons to Dicle University School of Medicine Physical Therapy and Rehabilitation Outpatient Clinic a total of 96 (78 female, 18 male) patients who were diagnosed with RA according to the 1987 American Collage of Rheumatology (ACR) criteria with the mean age of 46.97 ±13.44 years and 84 (36 male, 48 female) healthy control subjects with the mean age of 29.77± 16.99 years have been introduced to the study. All participants were evaluated in terms of clinical and laboratory parameters. HLA-DRB1 genotyping have been carried up by using PCR-SSP HLA-DRB1 Low Resolution gene panel. Subtypes of HLADRB1*01, *04, *07, *08, *10 were identified by PCR-SSP HLA-DRB1 High Resolution gene panel. Functionality and seropositivity of RA patients were also evaluated. Results: In RA patients types which have highest allel frequencies are DRB1*04 (%27.6), DRB1*11 (%14.6), and DRB1*15 (%13.5) in order; whereas, it was DRB1*11 (%24.4), DRB1*04 (%19.6), and DRB1*07 (%14.9) in the control group respectively. When allel frequecies of RA patients and control subjects were compared, the frequency of DRB1*10 allel was found to be higher, and DRB1*07 ve DRB1*11 allel frequencies were found to be significantly lower in RA patients (p<0.05). The comparison of HLADRB1 sub-types between groups showed significantly higher frequencies of DRB1*0401, *0408, and *1001 sub-types (p<0.05), and significantly lower frequencies of DRB1*0402, *0403, and *0701 sub-types in RA patients (p<0.05). There were no statistically significant association between seropositivity and allels and their sub-types (p>0.05). DRB1*10 allel was found to be associated with good functional status (stage 1-2); whereas, DRB1*11 was with poor functional status (stage 3-4) (p<0.05). Conclusions: In RA patients living in our area, DRB1*0401, *0408, *10, and *1001 allels might be associated with increased tendency to the disease; whereas, DRB1*0402, *0403,*0701, and *11 allels with decreased tendency to it. DRB1*10 and DRB1*11 allels may be related with disease severity in RA patients.
Açıklama
Anahtar Kelimeler
Alleller, Alleles, Artrit-romatoid, Arthritis-rheumatoid, HLA DR antijenleri, HLA DR antigens, Prevalans, Prevalence
