INVESTIGATION OF GLOBOZOOSPERMIA’S MORPHOLOGICAL STRUCTURE AND DNA FRAGMENTATION IN OLIGOZOOSPERMIA CASES IN INFERTILE MALES

Basit Öğe Kaydı
dc.contributor.authorYıldızhan, Eda
dc.contributor.authorÇankırı, Zuhal
dc.contributor.authorAkıncı, Dilara
dc.contributor.authorAşır, Fırat
dc.contributor.authorAfşin, Muhamet
dc.contributor.authorAkkuş, Murat
dc.contributor.authorDede, Onur
dc.date.accessioned2024-04-24T19:13:16Z
dc.date.available2024-04-24T19:13:16Z
dc.date.issued2022
dc.departmentDicle Üniversitesien_US
dc.description.abstractTotal globozoospermia is diagnosed by the presence of 100% round-headed spermatozoa without acrosomes. It is still unclear whether patients whose ejaculate contains both normal and globozoospermic cells (partial globozoospermia) suffer from a variation of the same syndrome. Affected men may experience decreased fertility and even infertility. In some cases, an increased number of cells with DNA fragmentation has also been observed in patients with globozoospermia..In this study, standard semen analysis methods in accordance with WHO criteria were applied to infertile male patient groups consisting of 20 normozoospermic and 20 oligozoospermic individuals who were admitted to our clinic. Age, sperm parameters (volume, vitality, concentration, total motility and morphology) were determined and statistically analyzed in normozoospermic and oligozoospermic infertile men.Sperms were stained with the Eosin-Nigrosin method and were visualized under an immersion lens light microscope and evaluated for vitality. The slides were stained using sperm staining solutions with the Spermac technique and the sperms were evaluated morphologically. Sperm DNA fragmentation damage was evaluated by acridine orange staining method.Our results revealed that sperm morphological features (Kruger test) and sperm DNA fragmentation, obtained with various staining techniques, are important in the clinical approach to male infertility and ART methods, and should be used together.en_US
dc.identifier.doi10.51477/mejs.1095136
dc.identifier.endpage63en_US
dc.identifier.issn2618-6136
dc.identifier.issue1en_US
dc.identifier.startpage56en_US
dc.identifier.trdizinid1102372
dc.identifier.urihttps://doi.org/10.51477/mejs.1095136
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1102372
dc.identifier.urihttps://hdl.handle.net/11468/28500
dc.identifier.volume8en_US
dc.indekslendigikaynakTR-Dizin
dc.language.isoenen_US
dc.relation.ispartofMiddle East Journal of Science
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleINVESTIGATION OF GLOBOZOOSPERMIA’S MORPHOLOGICAL STRUCTURE AND DNA FRAGMENTATION IN OLIGOZOOSPERMIA CASES IN INFERTILE MALESen_US
dc.titleINVESTIGATION OF GLOBOZOOSPERMIA’S MORPHOLOGICAL STRUCTURE AND DNA FRAGMENTATION IN OLIGOZOOSPERMIA CASES IN INFERTILE MALES
dc.typeArticleen_US

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu