Effects of Carvacrol on Experimental Testicular Torsion-Detorsion Model Investigation by Immunohistochemistry

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dc.contributor.authorDursun, Recep
dc.contributor.authorSen, Abdullah
dc.contributor.authorYaman, Mahmut
dc.contributor.authorDurgun, Hasan Mansur
dc.contributor.authorAsir, Firat
dc.date.accessioned2024-04-24T17:40:18Z
dc.date.available2024-04-24T17:40:18Z
dc.date.issued2020
dc.departmentDicle Üniversitesien_US
dc.description.abstractOBJECTIVE: To investigate the protective effects of carvacrol on an experimental testicular torsion-detorsion rat model. STUDY DESIGN: Wistar male rats (n=48) weighing 230-250 g were assigned to 4 groups (8 per group): control, torsion, torsion-detorsion, and torsion-detorsion + carvacrol-treated groups. Control group animals did not undergo any surgical operation. For the torsion group, the scrotum was opened (under general anesthesia) and the left testis twisted 720 degrees clockwise and in the last 30 minutes of 3-hour ischemia; i.p. saline was injected. In the torsion-detorsion group, after ischemia the left testis was reperfused for 2 hours. The torsion/ detorsion +carvacrol group protocol was similar to that of the torsion-detorsion group but in the last 30 minutes of 3-hour ischemia, i.p. 20 mg/kg carvacrol was administered. RESULTS: Malondialdehyde (MDA) was highest in the torsion-detorsion group (p <0.01). The lowest catalase (CAT) value was found in the torsion-detorsion group. Decrease in glutathione (GSH) levels of the torsion and torsion-detorsion groups as compared to those of control and carvacrol groups was significant (p<0.01). The highest superoxide dismutase (SOD) value was in the control and carvacrol groups. Increased apoptosis and degeneration of spermatogenic cells with hyperplasic nuclei were mainly observed in the torsion and torsion-detorsion groups. The torsion-detorsion + carvacrol group mostly showed regular histology, but Leydig cells were degenerated. ET-1 expression was increased in endothelial cells in the torsion and detorsion groups but negative in the carvacrol group. Bax expression was positive in luminal spermatogenic cells in the torsion group but negative in interstitial cells in both torsion and torsion-detorsion groups. In the carvacrol-treated group some luminal spermatogenic cells in seminiferous tubules showed positive Bax expression but weak in basal membrane cells and Leydig cells. CONCLUSION: Carvacrol influences spermatogenic cells with strong mitotic activity in basal membranes of seminiferous tubules and may prevent apoptotic development and signaling of these cells.en_US
dc.identifier.endpage132en_US
dc.identifier.issn0884-6812
dc.identifier.issue4en_US
dc.identifier.startpage125en_US
dc.identifier.urihttps://hdl.handle.net/11468/21739
dc.identifier.volume42en_US
dc.identifier.wosWOS:000592246800004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.language.isoenen_US
dc.publisherSci Printers & Publ Incen_US
dc.relation.ispartofAnalytical and Quantitative Cytopathology and Histopathology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBax Proteinen_US
dc.subjectBcl-2-Associated X Proteinen_US
dc.subjectCarvacrolen_US
dc.subjectDetorsionen_US
dc.subjectEndothelin-1en_US
dc.subjectIschemiareperfusion Injuryen_US
dc.subjectRatsen_US
dc.subjectWistaren_US
dc.subjectReperfusion Injuryen_US
dc.subjectSpermatic Cord Torsionen_US
dc.subjectTesticular Torsionen_US
dc.subjectTorsion Abnormalityen_US
dc.titleEffects of Carvacrol on Experimental Testicular Torsion-Detorsion Model Investigation by Immunohistochemistryen_US
dc.titleEffects of Carvacrol on Experimental Testicular Torsion-Detorsion Model Investigation by Immunohistochemistry
dc.typeArticleen_US

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