Improved in vitro propagation of Hyacinthus orientalis L. using fruits containing immature zygotic embryos and tender leaf sheath as explants

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dc.contributor.authorKizil S.
dc.contributor.authorSesiz U.
dc.contributor.authorKhawar K.M.
dc.date.accessioned2024-04-24T18:43:45Z
dc.date.available2024-04-24T18:43:45Z
dc.date.issued2016
dc.departmentDicle Üniversitesien_US
dc.description.abstractHyacinthus genus is an important group of ornamental plants that bear white, yellow, pink, red or purple coloured flowers. It has about 2000 species spread around the world that are grown commercially. Although, plant occurs naturally in Turkey yet efforts have not been made to adapt it for open field cultivation. There is need to transfer and es-tablish these plants from wild to fields for commercial use through in vitro and ex vitro approaches, that will help local economy profitably. This study reports in vitro culture of Hyacinthus orientalis L. subsp. orientalis; using fruits containing immature zygotic em-bryos cultured on MS medium containing varying concentrations of Thidiazuron (TDZ) with and without 0.2 mg l-1 naphthaleneacetic acid (NAA) supplemented with 20 or 40 g l-1 sucrose. The study also reports induction of bulblets on tender leaf sheaths on MS medi-um containing different concentrations of benzylaminopurine (BAP) + 0.1 mg l-1 NAA supplemented with 30 g l-1 sucrose. The maximum bulblet regeneration (40%) with 31.33 bulblets/explant was noted on MS medium containing 0.15 mg l-1 TDZ supple-mented with 40 g l-1 sucrose. Whereas, the best bulblet regeneration on tender leaf sheath explants was noted on 1.5 mg l-1 BAP + 0.1 mg l-1 NAA with 2.97 bulblets per explant of 0.55 cm bulb diameter and 1.20 leaves per bulblet. These bulblets were cultured singly on MS medium containing 20 mg l-1 GA3 (Gibberellic acid) + 50 g l-1 sucrose and attained a diameter of 0.75-1.00 cm in 30 days time. The bulbs regenerated on both explants were successfully rooted and acclimatised in plant growth chamber using peat moss followed by their transfer to open field conditions. © by Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie, Lublin 2016.en_US
dc.identifier.endpage30en_US
dc.identifier.issn1644-0692
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-84992051976en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage15en_US
dc.identifier.urihttps://hdl.handle.net/11468/24349
dc.identifier.volume15en_US
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherWydawnictwo Akad Rolniczej W Lublinieen_US
dc.relation.ispartofActa Scientiarum Polonorum, Hortorum Cultusen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFruits Contain-İng İmmature Zygotic Embryosen_US
dc.subjectHyacinthus Orientalis L. Subsp. Orientalisen_US
dc.subjectMicropropagationen_US
dc.subjectTender Leaf Sheathen_US
dc.titleImproved in vitro propagation of Hyacinthus orientalis L. using fruits containing immature zygotic embryos and tender leaf sheath as explantsen_US
dc.title.alternativeUlepszone rozmna?anie in vitro Hyacinthus orientalis L. przy u?yciu owoców zawieraj?cych niedojrza?e zarodki zygotyczne oraz pochwy li?ciowej jako eksplantówen_US
dc.typeArticleen_US

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