Presence of Clostridium spp. and Clostridium botulinum Types A, B, E, and F in Honey

dc.contributor.authorGuran, Husnu Sahan
dc.contributor.authorVural, Aydin
dc.contributor.authorErkan, Mehmet Emin
dc.date.accessioned2024-04-24T17:25:01Z
dc.date.available2024-04-24T17:25:01Z
dc.date.issued2023
dc.departmentDicle Üniversitesien_US
dc.description.abstractIn this study, a total of 300 honey samples were collected from different sale points in Diyarbakir, Mardin, Batman, Mus, Siirt, Bitlis, and Bingol cities in the Eastern and Southeastern Anatolian regions of Turkiye. The honey samples were analyzed for the presence of Clostridium spp., and Clostridium botulinum types A, B, E, and F. Supernatant filtration and dilution centrifuga-tion methods were used for the detection of Clostridium botulinum spores from honeys. Clostridium botulinum types A, B, E, and F were determined by multiplex real-time polymerase chain reaction. Out of 300 analyzed honey samples, 59 (19.6%) were contaminated with Clostridium spp. It was found that the highest amount of Clostridium spp. contamination belonged to the honey collected from Bingol with 28.5% and the Bitlis city had at lowest 12.5% (p > .05). However, Clostridium botulinum types A, B, E, and F were not detected in any of the samples including those confirmed as Clostridium spp. isolates by polymerase chain reaction. As a result, Clostridium spp. spo-res were determined to be at relatively low levels as none of the samples contained Clostridium botulinum spores. This situation can be considered as a result of the ongoing good production practices in the beekeeping industry in Turkiye.en_US
dc.description.sponsorshipScientific Research Project Fund (DUBAP) of Dicle University [17.017]en_US
dc.description.sponsorshipFunding:This study was supported by the Scientific Research Project Fund (DUBAP) of Dicle University under the project number Veteriner: 17.017.en_US
dc.identifier.doi10.5152/actavet.2023.22113
dc.identifier.endpage104en_US
dc.identifier.issn2618-639X
dc.identifier.issn2619-905X
dc.identifier.issue2en_US
dc.identifier.startpage99en_US
dc.identifier.urihttps://doi.org/10.5152/actavet.2023.22113
dc.identifier.urihttps://hdl.handle.net/11468/19908
dc.identifier.volume49en_US
dc.identifier.wosWOS:001015681700006
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoenen_US
dc.publisherAvesen_US
dc.relation.ispartofActa Veterinaria Eurasia
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectClostridium Botulinumen_US
dc.subjectClostridium Spp.en_US
dc.subjectDilution Centrifugeen_US
dc.subjectHoneyen_US
dc.subjectReal-Time Pcren_US
dc.subjectSupernatant Filtrationen_US
dc.titlePresence of Clostridium spp. and Clostridium botulinum Types A, B, E, and F in Honeyen_US
dc.titlePresence of Clostridium spp. and Clostridium botulinum Types A, B, E, and F in Honey
dc.typeArticleen_US

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