A cellulase-free, thermoactive, and alkali xylanase production by terrestrial Streptomyces sp CA24

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dc.contributor.authorPorsuk, Ilknur
dc.contributor.authorOzakin, Suleyman
dc.contributor.authorBali, Bulent
dc.contributor.authorYilmaz, Ebru Ince
dc.date.accessioned2024-04-24T17:21:02Z
dc.date.available2024-04-24T17:21:02Z
dc.date.issued2013
dc.departmentDicle Üniversitesien_US
dc.description.abstractMicrobial xylanases have attracted much attention because of their applications in industrial processes during the past few decades. Sixty-five terrestrial actinomycetes previously isolated from rhizospheric soils of endemic plants were tested for their ability in production of xylanase. The results revealed that 33 isolates could degrade xylan on both solid and liquid media. Extracellular xylanase production by these isolates was characterized during their growth in liquid medium. Streptomyces sp. CA24, one of the most active strains, was selected for optimization of xylanase production. The enzyme secretion was enhanced when the medium was supplemented with oat spelt xylan (0.5%) and yeast extract (0.5%) at pH 10.0. Optimization of the culture conditions resulted in a 5-fold enhancement in enzyme production (255 U/mL). The most important characteristic of the enzyme is its production over a broad pH range of 6.0-10.0 during all stationary phases. The cellulase-free xylanase was optimally active at 60 degrees C and almost 90% stable after 4 h at 50 degrees C. The high levels of enzyme production obtained under alkali batch cultivation conditions, coupled with no production of cellulase, suggests the use of the local terrestrial strain Streptomyces CA24 and/or its enzyme in industrial processes such as pulp and paper production.en_US
dc.identifier.doi10.3906/biy-1208-40
dc.identifier.endpage375en_US
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue3en_US
dc.identifier.startpage370en_US
dc.identifier.urihttps://doi.org/10.3906/biy-1208-40
dc.identifier.urihttps://hdl.handle.net/11468/19344
dc.identifier.volume37en_US
dc.identifier.wosWOS:000321227900016
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectStreptomycesen_US
dc.subjectXylanaseen_US
dc.subjectMedium Optimizationen_US
dc.subjectAlkali-Thermoactive Enzymeen_US
dc.titleA cellulase-free, thermoactive, and alkali xylanase production by terrestrial Streptomyces sp CA24en_US
dc.titleA cellulase-free, thermoactive, and alkali xylanase production by terrestrial Streptomyces sp CA24
dc.typeArticleen_US

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