Amylase Production of Bacillus subtilis Isolated from Soil by SmF Method

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dc.contributor.authorOrtakaya, Veysi
dc.contributor.authorFincan, Sema Agüloğlu
dc.date.accessioned2024-04-24T19:11:35Z
dc.date.available2024-04-24T19:11:35Z
dc.date.issued2019
dc.departmentDicle Üniversitesien_US
dc.description.abstractIn our study, the isolation, identification and optimization of Bacillus subtilis from soil samples were performedand the ability to produce ?-amylase of industrial importance was investigated. Bacteria identified as B. subtilis by 16SrRNA analysis, morphological and biochemical tests. Maximum growth was determined at 32 hour, 37°C and pH 7.0.Enzyme optimization was performed after determination of bacterial amylase in starchy medium. Optimum conditionsfor amylase production were determined as 37°C, pH 7.0 and 48 h. When the effect of metals on enzyme activity wasexamined, it was determined that Cu2+ increased enzyme activity, Zn2+, Hg2+ and Fe2+ were partially inhibited theenzyme activity, and Ca2+ was showed close activity to the control. It was observed that carbon sources had inhibitoryeffect on bacterial growth and enzyme production.en_US
dc.identifier.doi10.5505/biodicon.2019.70288
dc.identifier.endpage64en_US
dc.identifier.issn1308-5301
dc.identifier.issn1308-8084
dc.identifier.issue3en_US
dc.identifier.startpage57en_US
dc.identifier.trdizinid318400
dc.identifier.urihttps://doi.org/10.5505/biodicon.2019.70288
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/318400
dc.identifier.urihttps://hdl.handle.net/11468/28101
dc.identifier.volume12en_US
dc.indekslendigikaynakTR-Dizin
dc.language.isoenen_US
dc.relation.ispartofBiological Diversity and Conservation
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleAmylase Production of Bacillus subtilis Isolated from Soil by SmF Methoden_US
dc.titleAmylase Production of Bacillus subtilis Isolated from Soil by SmF Method
dc.typeArticleen_US

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