Investigation of the effects of sildenafil on liver and remote organ in hepatic ischemia-reperfusion damage

Basit Öğe Kaydı
dc.contributor.authorCetin, Erman
dc.contributor.authorOguz, Abdullah
dc.contributor.authorBasol, Omer
dc.contributor.authorBilge, Huseyin
dc.date.accessioned2024-04-24T17:24:14Z
dc.date.available2024-04-24T17:24:14Z
dc.date.issued2020
dc.departmentDicle Üniversitesien_US
dc.description.abstractIntroduction The aim of this study was to demonstrate whether sildenafil is effective in minimizing and/or eliminating hepatic ischemia/reperfusion injury effects. For this purpose, the authors experimentally performed biochemical and histopathological examinations of the included rats using the hepatic ischemia/reperfusion model. Materials and methods The authors used 40 animals, with 10 rats in each group, in this study. Ischemia was applied 30-45min with the hepatoduodenal ligament clamping, and then reperfusion is started. The rats were grouped as follows: the first group, only laparotomy; the second group, laparotomy and sildenafil; the third group, hepatic ischemia-reperfusion; and the fourth group, hepatic ischemia-reperfusion and sildenafil. During experimental studies, sildenafil capsules were opened, and appropriate dose required for animals had been created with the weighing scales. Then, the powder was diluted with saline. The authors gave sildenafil through oral gavage 15 minutes before the ischemia. 60min after starting the experiment in 1-2 groups and 30min after beginning reperfusion in 3-4 groups (60min after beginning the experiment in all groups), blood was taken from the animals for biochemical analysis, and the animals were sacrificed. Simultaneously liver, lung, and kidney tissues were removed for biochemical and histopathological examination. Results Based on plasma evaluation, total antioxidant status was lower (P=0.0274) in ischemia/reperfusion group compared with ischemia/reperfusion+sildenafil group. However, there was no difference between the groups regarding total oxidant status values (P=0.0274). When comparing total antioxidant status and oxidative stress index in liver tissue, a statistically significant difference was observed between groups (P=0.012766 and P=0.004081), but on comparing histopathological scores, there was no difference between groups (P=0.1244). Conclusion Sildenafil partly reduced the effects of hepatic ischemia-reperfusion injury on the liver and distant organs, although this difference was not statistically significant.en_US
dc.identifier.doi10.4103/ejs.ejs_219_20
dc.identifier.endpage1189en_US
dc.identifier.issn1110-1121
dc.identifier.issn1687-7624
dc.identifier.issue4en_US
dc.identifier.startpage1183en_US
dc.identifier.urihttps://doi.org/10.4103/ejs.ejs_219_20
dc.identifier.urihttps://hdl.handle.net/11468/19551
dc.identifier.volume39en_US
dc.identifier.wosWOS:000607808400050
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoenen_US
dc.publisherWolters Kluwer Medknow Publicationsen_US
dc.relation.ispartofEgyptian Journal of Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHepatic Ischemia-Reperfusionen_US
dc.subjectLiveren_US
dc.subjectRemote Organen_US
dc.subjectSildenafilen_US
dc.titleInvestigation of the effects of sildenafil on liver and remote organ in hepatic ischemia-reperfusion damageen_US
dc.titleInvestigation of the effects of sildenafil on liver and remote organ in hepatic ischemia-reperfusion damage
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu