Modicogryllus truncatus'ta (Tarbinsky,1940) (Orthoptera: Gryllidae) aşırı doymamış yağ asitlerinin biyosentezi
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Tarih
2017
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Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, Modicogryllus truncatus'ta (Orthoptera:Gryllidae) linoleik, linolenik asitler gibi 18 karbonlu aşırı doymamış yağ asitleri ile eikosatrienoik, eikosatetraenoik ve eikosapentaenoik asitler gibi eikosanoid öncül maddeleri olan 20 karbonlu aşırı doymamış yağ asitlerinin biyosentezi araştırıldı. Bunun için nimfler ergin oluncaya kadar, marul içeren stok kültür ortamı, yağsız sentetik besin ve buğday tohum yağı içeren sentetik besin üzerinde yetiştirildiler. Bu farklı besinler üzerinde yetiştirilen M. truncatus'un bir günlük ergin erkek ve dişi bireylerinin fosfolipit ve triaçilgliserol yağ asiti kompozisyonları gaz kromatografi ve kütle spektrometresi ile ayrı ayrı analiz edilmiştir. Böceklerin fosfolipit ve triaçilgliserol fraksiyonlarında palmitik, oleik ve linoleik asitler yüzde dağılımında en yüksek miktarda bulunmuştur. Ayrıca çalışmada pentadekanoik (15:0) ve heptadekanoik (17:0) asitler gibi tek karbonlu yağ asitleri de tespit edilmiştir. Linolenik (18:3n-3) asit ile eikosanoidlerin öncül maddeleri olan eikosatetraenoik (20:4n-6) ve eikosapentaenoik (20:5n-3) asit gibi 20 karbonlu aşırı doymamış yağ asitleri hem fosfolipit hem de triaçilgliserol fraksiyonunda az miktarda da olsa tespit edilmiştir. Araştırmamızın sonuçlarına göre M. truncatus'ta linoleik (18:2n-6), linolenik (18:3n-3), eikosatetraenoik (20:4n-6) ve eikosapentaenoik asitin (20:5n-3) de novo biyosentezi ve eikosatetraenoik asitin zincir uzatma- desaturasyonu tespit edilmiştir.
In this study, C18 polyunsaturated fatty acids such as linoleic, linolenic acids and eicosanoids precusors C20 polyunsaturated fatty acids such as eicosatrienoic, eicosatetraenoic and eicosapentaenoic acids biosynthesis in M. truncatus (Orthoptera:Gryllidae) were investigated. For this, nymphs were reared separately on three different diets such as stock culture medium containing lettuce, fat-free artifical diet and artifical diet containing wheat germ oil until to adult. The phospholipid and triacylglycerol fatty acid composition of one-day old adult male and female M. tuncatus reared on three different diets were analysed separately by gas chromatography-mass spectrometry The analysis of fatty acid composition of insects showed that palmitic, oleic and linoleic acids were found to be major fatty acids in phospholipid and triacylglicerol fractions. In addition, our analysis showed that presence of odd-chain fatty acids such as pentadecanoic (15:0) and heptadecanoic (17:0) acids. Trace amounts of linolenic and eicosanoid precusors C20 polyunsaturated fatty acids such as eicosatetraenoic and eicosapentaenoic acids were detected in both fractions. According to our results, de novo biosynthesis of linoleic (18:2n-6), linolenic (18:3n-3), eicosatetraenoic (20:4n-6) and eicosapentaenoic (20:5n-3) acids and elongation-desaturation of eicosatetraenoic acid (20:4n-6) in M. truncatus were demonstrated.
In this study, C18 polyunsaturated fatty acids such as linoleic, linolenic acids and eicosanoids precusors C20 polyunsaturated fatty acids such as eicosatrienoic, eicosatetraenoic and eicosapentaenoic acids biosynthesis in M. truncatus (Orthoptera:Gryllidae) were investigated. For this, nymphs were reared separately on three different diets such as stock culture medium containing lettuce, fat-free artifical diet and artifical diet containing wheat germ oil until to adult. The phospholipid and triacylglycerol fatty acid composition of one-day old adult male and female M. tuncatus reared on three different diets were analysed separately by gas chromatography-mass spectrometry The analysis of fatty acid composition of insects showed that palmitic, oleic and linoleic acids were found to be major fatty acids in phospholipid and triacylglicerol fractions. In addition, our analysis showed that presence of odd-chain fatty acids such as pentadecanoic (15:0) and heptadecanoic (17:0) acids. Trace amounts of linolenic and eicosanoid precusors C20 polyunsaturated fatty acids such as eicosatetraenoic and eicosapentaenoic acids were detected in both fractions. According to our results, de novo biosynthesis of linoleic (18:2n-6), linolenic (18:3n-3), eicosatetraenoic (20:4n-6) and eicosapentaenoic (20:5n-3) acids and elongation-desaturation of eicosatetraenoic acid (20:4n-6) in M. truncatus were demonstrated.
Açıklama
Anahtar Kelimeler
Biyoloji, Biology, Biyosentez, Doymamış yağ asitleri
