Comparison of Two Different Media for In vitro Production of Dog Embryos

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dc.contributor.authorEvecen, Mithat
dc.contributor.authorCirit, Umut
dc.contributor.authorDemir, Kamber
dc.contributor.authorKaraman, Elif
dc.contributor.authorBakirer, Gul
dc.contributor.authorHamzaoglu, Asiye Izem
dc.contributor.authorBirler, Sema
dc.date.accessioned2024-04-24T17:47:43Z
dc.date.available2024-04-24T17:47:43Z
dc.date.issued2010
dc.departmentDicle Üniversitesien_US
dc.description.abstractEmbryo production via in vitro fertilization and nuclear transfer has been accomplished in the dog, and the transfer of the cloned embryos has recently resulted in the birth of puppies. However, the efficiency of these technologies is still very limited. Until now, only two morulas and single blactocyst have been achieved in vitro. Therefore, the aim of the present study was to examine the effects of two different media (mSOF and TCM 199) on in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture (IVC) of immature dog oocytes. The study was performed in two steps. At the first step, the effects of two different media, on IVM of dog oocytes were investigated. At the end of the IVM period, the nuclear maturation rates were evaluated by aceto-orcein staining method. At the second step, after the IVM period the oocytes inseminated with fresh spermatozoa for 24 h and left for IVC for 7 d. At the end of the IVC period, embryonic development was assessed by microscopic observation at 24 h intervals and then fixed and stained by the same method. Consequently, maturation rates of oocytes in mSOF medium were significantly higher than those of the TCM 199 (P<0.001). After 7 d of the IVC period, only one oocyte was cleaved in the TCM 199. while eight oocytes cleaved and one of them developed to morula stage in mSOF medium group (P=0.037).en_US
dc.description.sponsorshipIstanbul University [1145]en_US
dc.description.sponsorshipThe present work was supported by the Research Fund of Istanbul University (Project No: 1145)en_US
dc.identifier.endpage902en_US
dc.identifier.issn1300-6045
dc.identifier.issue6en_US
dc.identifier.startpage897en_US
dc.identifier.urihttps://hdl.handle.net/11468/22705
dc.identifier.volume16en_US
dc.identifier.wosWOS:000285216200001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.language.isoenen_US
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisien_US
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDogen_US
dc.subjectOocyteen_US
dc.subjectIn Vitroen_US
dc.subjectMaturationen_US
dc.subjectFertilizationen_US
dc.titleComparison of Two Different Media for In vitro Production of Dog Embryosen_US
dc.titleComparison of Two Different Media for In vitro Production of Dog Embryos
dc.typeArticleen_US

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