Assessment of the results of erlich ziehlneelsen and fluorochrome staining procedures, bactec 460 and löwenstein-jensen culturing procedures in the diagnosis of tuberculosis

dc.contributor.authorMete, Mahmut
dc.contributor.authorÖzekinci, Tuncer
dc.contributor.authorSuay, Adnan
dc.contributor.authorAkpolat, Nezahat
dc.contributor.authorAtmaca, Selahattin
dc.date.accessioned2024-04-24T17:56:18Z
dc.date.available2024-04-24T17:56:18Z
dc.date.issued2004
dc.departmentDicle Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Mikrobiyoloji Ana Bilim Dalıen_US
dc.description.abstractThe Ziehl-Neelsen (ZN) and fluorochrome (FC) staining procedures and the Bactec and Löwenstein-Jensen (L-J) culturing procedures were used to test 340 clinical specimens for tuberculosis, and the diagnostic value of the fluorochrome procedure was investigated. Positive cultures were obtained from 34 specimens (10%), of which 13 (3.8%) tested positive in ZN, and 18 (5.3%) in FC. Sensitivity of the ZN and FC staining results was found to be 38.2% and 52.9%, respectively. NAP (p-nitro-?-acetylamino-?-hidroxypropiophenone) identified 32 (94.1%) of the 34 strains as M. tuberculosis complex, and 2 (5.9%) as Mycobacteria other than tuberculosis (MOTT) bacilli. Twenty-one (61.7%) of the 34 culture-positive specimens grew only in Bactec 12 B medium, 2 (5.9%) grew only in L-J medium, and 11 (32.3%) grew in both Bactec and L-J media. The 32 M. tuberculosis complex strains' sensitivities to streptomycin (STR), isoniazid (INH), rifampin (RIF), and ethambutol were assessed with the Bactec system. Four (12.5%) of these strains were resistant to streptomycin, 9 (28.1%) to isoniazid, 7 (21.8%) to rifampin, and 6 (18.7%) to ethambutol. Total drug resistance was 43.7%. Six strains (18.7%) were resistant to 1 drug, 5 (15.6%) to 2 drugs, 2 (6.2%) to 3 drugs, and 1 (3.1%) to all 4 drugs, isoniazid plus rifampin resistance was seen in 18.7%.en_US
dc.identifier.citationMete, M., Özekinci, T., Suay, A. ve Akpolat, N. (2004). Assessment of the results of erlich ziehlneelsen and fluorochrome staining procedures, bactec 460 and löwenstein-jensen culturing procedures in the diagnosis of tuberculosis. Biotechnology and Biotechnological Equipment, 18(3), 165-169.
dc.identifier.doi10.1080/13102818.2004.10817139
dc.identifier.endpage169en_US
dc.identifier.issn1310-2818
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85023991087
dc.identifier.scopusqualityQ3
dc.identifier.startpage165en_US
dc.identifier.urihttps://doi.org/10.1080/13102818.2004.10817139
dc.identifier.urihttps://hdl.handle.net/11468/23437
dc.identifier.urihttps://www.tandfonline.com/doi/abs/10.1080/13102818.2004.10817139
dc.identifier.volume18en_US
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.relation.ispartofBiotechnology and Biotechnological Equipment
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleAssessment of the results of erlich ziehlneelsen and fluorochrome staining procedures, bactec 460 and löwenstein-jensen culturing procedures in the diagnosis of tuberculosisen_US
dc.titleAssessment of the results of erlich ziehlneelsen and fluorochrome staining procedures, bactec 460 and löwenstein-jensen culturing procedures in the diagnosis of tuberculosis
dc.typeArticleen_US

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