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  • Küçük Resim
    Öğe
    Immunohistochemical analysis of vimentin and Zonula Occludens-1 in placentas of patients with PPROM
    (IMR Press Limited, 2024) Zaman, Fuat; Aşır, Fırat; Ermiş, Işılay Sezen; Tuncer, Mehmet Cudi; Deveci, Engin; Oğlak, Süleyman Cemil
    Background: We aimed to investigate the immunohistochemical staining of vimentin and zonula occludens-1 (ZO-1) expression in the placentas of pregnant women with preterm premature rupture of membranes (PPROM). Methods: Placentas of 25 healthy and 25 women with PPROM were fixed in 10% formaldehyde solution and further processed for paraffin wax tissue embedding. Demographic properties of patients were recorded. Placentas were histologically stained with hematoxylin-eosin and vimentin and ZO-1 expression immunostaining. Results: Vimentin expression was high in the decidual cells, fibroblasts, and connective tissue fibers in control group. Compared to control group, vimentin expression was decreased in the placental structures of PPROM group, where fetal membranes were degenerated and histologically irregular. Similar to vimentin expression, ZO-1 expression was also high in placental components of control group such as chorioamniotic membrane and amniotic epithelium. The PPROM group showed lower expression of ZO-1 expression in placental structures than in that of control. ZO-1 expression was significantly lowered in regions where fetal membrane integrity was weakened and lost. Conclusions: We suggest that ZO-1 and vimentin expression may show alteration in etiology premature rupture of membrane.
  • Küçük Resim
    Öğe
    Polikistik over sendromlu olgularda insülin rezistansının insülin tolerans testi ile araştırılması
    (Dicle Üniversitesi, Tıp Fakültesi, 2002) Zaman, Fuat; Bayhan, Gökhan; Yalınkaya, Ahmet
    Şubat 2001 ile Nisan 2002 tarihleri arasında Dicle Üniversitesi Tıp Fakültesi (DÜTF) Kadın Hastıkları ve Doğum Anabilim Dalı polikliniğine hirsutizm ve adet düzensizliği şikayetleri ile gelen ve polikistik over sendromu (PCOS) tespit edilen 61 olgu ile 28 normal olan olgu kontrol grubunu oluşturmak üzere çalışma kapsamına alındı. Olgular hiperinsülinemi, hiperandrojenemi ve insülin resistansı yönünden incelendi. Her iki grubunda yaş ortalamaları benzerdi. Tüm olguların bazal glukoz, insülin, PRL, LH, TT, FT, SHBG, A düzeylerine bakılarak, hepsine insülin tolerans testi yapıldı. Olguların hirsutism derecesi Ferriman-Gallwey skalasına göre belirlendi, 8 ve üstü hirsutism kabul edildi. Çalışma grubu ile kontrol grubunun hormon düzeyleri arasında istatistiksel anlamlı fark bulundu. Olguların AKŞ, ITT ve BMI'lerinin ortalamaları aralarında istatistiksel anlamlı fark bulumamasına karşın, çalışma grubunda daha yüksek bulundu. Çalışma grubunun 5'inde şiddetli, 6'sında hafif olmak üzere 11 (%18)'inde insülin resistansı tespit edildi. PCOS'lu, insülin ve androjen düzeyleri yüksek, ailesinde tip II diabet öyküsü olan ve tedaviye dirençli hirsutizm olgularında insülin rezistansının araştırılması gerektiği düşünmekteyiz. Bu olgularda insülin rezistansının araştırılmasında, insülin tolerans testinin (ITT) diğer testlerden daha pratik, kısa zamanda ve her yerde kolay yapılabilen, yan etkisi az ve doğru sonuç veren, güvenilir bir test olarak değerlendirmekteyiz.
  • [ X ]
    Öğe
    Protective Effect of Folic Acid on Deltamethrin-induced Brain Toxicity during the Pregnancy Period in Rats
    (Colegio Farmaceuticos Provincia De Buenos Aires, 2024) Behram, Mustafa; Zaman, Fuat; Oglak, Sueleyman Cemil; Deveci, Engin
    This study aimed to investigate the ameliorative effects of folic acid on deltamethrin-induced brain toxicity during the pregnancy period by histopathological, immunohistochemical, and biochemical methods in rats. A total of 32 female Wistar rats were used in the study. The animals were divided into 4 groups as follows: Control (n=8), Deltamethrin (DTM) (n=8) (0.5 mL of 5 mg/kg BW), Folic acid (n=8) (50 mu g/kg/day), Folic acid+ Deltamethrin (n=8) groups. After the animals mating, the first day of pregnancy was determined by vaginal smears. DTM and folic acid were administered orally between 6-21 days. At the end of the 21st day of pregnancy, brain tissues were excised and malondialdehyde (MDA), catalase (CAT), and glutathione (GSH) levels were determined from the homogenized tissue pieces. The remaining tissue pieces were fixed in neutral formaldehyde for histopathological observations. TNF-alpha staining was used for immunohistochemical analysis. TUNEL assay analysis was used to examine the DNA fracture of apoptotic cells. Brain MDA levels were increased in the DTM group compared to the control group (p < 0.01). Folic acid was found to cause a decrease in lipid peroxidation. GSH and CAT levels decreased compared to DTM in the control group (p < 0.05). TNF-alpha expression in small accumulations of microglia cells with macrophage characteristics was observed in the DTM group. Cells with negative TUNEL reaction were detected in many areas in the DTM and folic acid -treated group while the apoptotic index was significantly increased in the DTM group. Increased inflammation, induced oxidative stress, and increased apoptotic index in pup rats due to maternal DTM toxicity caused neuronal and glial cell damage. Folic acid is thought to play an important role in preventing cell damage due to its free radical scavenging activity and anti-apoptotic effect on inflammation reduction.