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Öğe AUTHOR REPLY TO: Mean platelet volume in pseudoexfoliation syndrome and glaucoma(Wichtig Editore, 2014) Turkcu, Fatih Mehmet; Yuksel, Harun; Sahin, Alparslan; Cinar, Yasin; Yuksel, Hatice; Cingu, Kursat; sahin, Muhammed[Abstract Not Available]Öğe Carvacrol prevents methotrexate-induced renal oxidative injury and renal damage in rats(Canadian Soc Clinical Investigation, 2014) Bozkurt, Mehtap; Em, Serda; Oktayoglu, Pelin; Turkcu, Gul; Yuksel, Hatice; Sariyildiz, Mustafa A.; Caglayan, MehmetPurpose: The purpose of this study was to investigate the effect of carvacrol (CAR) on methotrexate (MTX)-induced renal damage in rats. Methods: Twenty-four male rats were equally divided into three groups: group I, control treatment; group II, MTX-treated; and group III, MTX+CAR-treated. A single dose of CAR (73 mg/kg) was administered intraperitoneally to group III on the first day of the experiment and a single dose of MTX (20 mg/kg) was administered intraperitoneally to groups II and III on the second day of the experiment. Blood samples and kidney tissue were obtained from each animal on day 8 for the measurement of malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS), and oxidative stress index (OSI). Light microscopy was used for histopathological examination of kidney specimens. Results: MDA, TOS and OSI levels were significantly greater in the group receiving MTX alone relative to the control animals, while the TAS level was significantly reduced in the MTX group compared with the control group. The administration of CAR was associated with signicantly decreased MDA, TOS, and OSI levels and increased TAS levels relative to the rats treated with MTX alone. Animals treated with CAR exhibited decreased tubular degeneration and architectural impairment relative to animals treated with MTX alone; however, the difference in histological scores did not meet the threshold of statistical significance. Conclusions: MTX treatment results in oxidative damage to the rat kidney; damage which is partially abrogated by the administration of CAR.Öğe Effect of Caffeic Acid Phenethyl Ester on Cerebellar Tissue Damage Secondary to Methanol Intoxication: Experimental Study(Turkish Neurological Soc, 2013) Arikanoglu, Adalet; Yuksel, Hatice; Gocmez, Cuneyt; Uzar, Ertugrul; Acar, Abdullah; Aluclu, Mehmet UfukObjective: Previous studies have shown the role of oxidative stress in methanol neurotoxicity. CAPE is known to have an antioxidant property that is shown in many experimental studies. In this study, we aimed to investigate whether CAPE has a protective effect against oxidative stress observed in the cerebellar tissue in methanol intoxication. Material and Method: In this study, a total of 40 rats were split into 5 groups: control group (n=8), MTX-alone group (n=8), MTX+methanol group (n=8), MTX+methanol+ethanol group (ethanol group) (n=8), and MTX+metanol+CAPE group (CAPE group) (n=8). All the rats except the control group were delivered methotrexate (MTX) therapy (0.3 mg/kg/day, via i. p. route) for 7 days in order to induce methanol toxicity. The control group received no drug therapy. Seven days later, 3 g/kg (i.p.) methanol was delivered in the ethanol and CAPE groups. Four hours after the delivery of methanol, ethanol group received 0.5 g/kg ethanol (i.p.) and CAPE group received 10 mu mol/kg CAPE (i.p.), while the other groups were delivered only saline (i.p.). The rats were decapitated after 8 hours and the cerebellar tissues were removed. PON-1, TAS, and MDA levels were measured in the tissues. Results: MTX-alone group demonstrated decreased TAS and PON-1 levels (p=0.001 and p=0.004, respectively) and increased MDA level (p=0.001), as compared to the control group. When MTX+methanol group was compared with the MTX-alone group, MTX+methanol group was found to have decreased TAS and PON-1 activities (p=0.037 and p=0.046, respectively) and increased MDA level (p=0.022). The ethanol group was found to show a significant decrease in MDA level (p=0.001), as compared with the MTX+methanol group. The CAPE group exhibited increased TAS and PON-1 levels (p=0.001 and p=0.001, respectively) and decreased MDA levels, as compared with the MTX+methanol group. Discussion: Cerebellum demonstrates oxidative stress secondary to methanol intoxication. CAPE therapy is more effective against cerebellar oxidative stress than ethanol therapy.Öğe Effect of Estrogen Replacement Treatment on VEGF in Serum and Retina in Rats(Modestum Ltd, 2015) Yuksel, Harun; Turgut, Fethiye Gulden; Turkcu, Fatih M.; Ozkurt, Zeynep; Sahin, Muhammed; Yuksel, Hatice; Turkcu, GulVascular endothelial growth factor (VEGF) is a molecule implicated in the pathogenesis of several eye diseases. In this experimental study, we planned to evaluate the effects of surgical menopause and hormone replacement therapy on VEGF levels. Thus, we studied the effects of treatments involving estrogen, estrogen and progesterone (E/P) in combination, and genistein, which is a selective estrogen modulator, on serum VEGF levels and the expression of VEGF in the retinas of rats with surgical menopause. The rats were randomly divided into five groups. Bilateral ovariectomy (OVX) was performed in all groups except for the sham-operated group. estrogen, E/P, genistein or water (sham and control groups) treatments were given for 8 weeks through the orogastric catheter. Serum VEGF level and immunohistochemical staining of VEGF in retinal tissue were analyzed in each group. Serum VEGF levels were significantly higher in the OVX + estrogen and OVX + genistein groups than in the control and sham groups. It was also higher in the OVX + E/P group than in the sham and control groups; however, in this case, the difference was not statistically significant. The OVX + estrogen, OVX + E/P, and OVX + genistein groups exhibited increased VEGF staining in comparison with the control and sham groups. However, the difference was not statistically significant. The current study demonstrated that estrogen replacement treatment (ERT) leads to an increase in serum VEGF levels and progesterone plays a protective role in this increase. The ERT used in this study had no effect on VEGF expression in the retina.Öğe The effects of caffeic acid phenethyl ester in acute methanol toxicity on rat retina and optic nerve(Taylor & Francis Ltd, 2013) Sahin, Alparslan; Kaya, Savas; Turkcu, Gul; Cingu, Abdullah Kursat; Yuksel, Harun; Turkcu, Fatih Mehmet; Yuksel, HaticePurpose: We aimed to test caffeic acid phenethyl ester (CAPE) as an antidote for acute methanol (MeOH) toxicity and to compare it with ethanol. Methods: This study included five groups, each containing eight rats. The groups were control, methotrexate (MTX), MeOH, ethanol and CAPE. All rats except control group were treated with intraperitoneal (i.p.) MTX (0.3 mg/kg/d) for 7 d. At the 8th day of the experiment, i.p. injection of MeOH (3 g/kg) was administered in MeOH, ethanol and CAPE groups. Four hours after MeOH treatment, 0.5 g/kg ethanol was injected i.p. in ethanol group; 10 mu mol/kg CAPE i.p. in CAPE group; serum physiologic i.p. in other groups. After 8 h, rats were anaesthetized and sacrificed. Total anti-oxidant status (TAS), total oxidant status (TOS) were measured on the dissected and excised retina and optic nerve samples. Fellow eyes were used for histopathologic evaluation and the cell count of retinal ganglion cell (RGC) layer. In addition, interactions of alcohol dehydrogenase with CAPE, ethanol, MeOH and pyrazole derivatives were investigated. Results: Either CAPE or ethanol co-treatment decreased the TOS levels and increased the TAS levels compared to the MeOH group. MeOH treatment decreased the mean cell count in RGC layer. CAPE co-treatment significantly prevented cell loss (p=0.040). Besides, in silico calculations showed that binding affinity of CAPE to alcohol dehydrogenase was higher than those of MeOH, ethanol, and pyrazole derivatives were. Conclusion: This study demonstrated that CAPE treatment decreased the oxidative stress in acute MeOH intoxication in the retina and optic nerve; beside that, protected RGC layer histology. In silico, CAPE had higher affinity score than MeOH, ethanol, pyrazole and pyrazole derivatives in the case of interaction with alcohol dehydrogenase.Öğe Effects of Ellagic Acid on Copper, Zinc, and Biochemical Values in Serum and Liver of Experimental Cholestatic Rats(Humana Press Inc, 2011) Gumus, Metehan; Yuksel, Hatice; Evliyaoglu, Osman; Kapan, Murat; Boyuk, Abdullah; Onder, Akin; Aldemir, MustafaEllagic acid (EA) is a natural polyphenolic compound. Although, modulator effects of EA on copper (Cu) and zinc (Zn) levels in some liver diseases have been reported in experimental animals, its effects in obstructive jaundice (OJ) has not been clarified. We aimed to evaluate potential effects of EA on Cu and Zn levels in liver and serum of cholestatic rats. Forty Wistar albino rats were equally divided into four groups. First group was used as controls. Second group received EA (60 mg(-1) kg(-1) day(-1)) for 8 days. Third was OJ group, and fourth group was OJ plus EA group. After 8 days, blood and liver samples were obtained. Higher serum and liver Cu and lower serum and liver Zn levels were found in OJ group (p < 0.05) compared with other groups. However, these differences reached to significant levels for Cu in serum and for Zn in lever. Higher serum copper levels were decreased, and lower liver Zn levels were increased by EA treatment in cholestatic rats (p < 0.05). Also, higher Cu/Zn ratio in OJ group was decreased by EA treatment both in liver (p < 0.05) and in serum (p < 0.05). Significantly higher serum bilirubin, alkaline phosphatase, alanine aminotransferase, and aspartate aminotransferase values were found in OJ and OJ + EA groups compared with the control and EA groups (p < 0.05). In conclusion, result of the current study indicated that ellagic acid has modulator effects on Cu and Zn levels in liver and serum of cholestatic rats.Öğe The effects of erythropoietin on bacterial translocation and inflammation in rats with obstructive jaundice(Edizioni Luigi Pozzi, 2014) Onder, Alin; Kapan, Murat; Yuksel, Hatice; Tekin, Recep; Kele, Ayserur; Evliyaoglu, Osman; Arikanoglu, ZulfuINTRODUCTION: Obstruction of the common bile duct is associated with hepatic paranchymal damage and increased susceptibility to subsequent bacterial infections. Erythropoietin has antiinflammatory and cytoprotective effects and it induces antiinflammatory cytokines and suppresses the production of proinflammatory cytokines. In this study, we aimed to investigate the effect of Erythropoietin on bacterial translocation, inflammation and tissue damage in rats with obstructive jaundice. MATERIALS AND METHODS: Thirty-two Wistar albino rats (200-250 g) were divided into 4 groups as follows: Group 1 (Sham); only hepatoduodenal ligament dissection, Group 2 (Erythropoietin); hepatoduodenal ligament dissection and given 500 IU/kg Erythropoietin subcutaneously, Group 3 (Obstructive jaundice); complete hepatoduodenal ligament ligation, Group 4 (Obstructive jaundice + Erythropoietin); complete hepatoduodenal ligament ligation and given 500 IU/kg Erythropoietin subcutaneously. After 7 days, the rats were sacrificed by taking blood from the heart for biochemical analyses. Peritoneal swab culture, liver, mesenteric lymph nodes, spleen and ileum were collected for microbiological and histopathological examinations. RESULTS: Erythropoietin reduced the secretion of inflammatory cytokines, oxidative damage and bacterial translocation, prevent the formation of inflammatory changes in intestine and liver after obstructive jaundice. Conclusion: The treatment of EPO in rats with Of reduces bacterial translocation, inflammation and tissue damage.Öğe THE EFFECTS OF ERYTHROPOIETIN ON BACTERIAL TRANSLOCATION AND INFLAMMATORY RESPONSE IN AN EXPERIMENTAL INTESTINAL OBSTRUCTION MODEL IN RATS(Soc Medical Biochemists Serbia, 2013) Kapan, Murat; Onder, Akin; Yuksel, Hatice; Evliyaoglu, Osman; Firat, Ugur; Tekin, Recep; Gul, MesutBackground: Intestinal obstruction results in distortion of balance of antiinflammatory cytokines and release of oxidants, and also leads to bacterial translocation, sepsis and multiple organ failure. Asymmetric dimethylarginine is related to multiple organ failure as a new prognostic marker. Erythropoietin reduces the inflammatory response by decreasing the levels of proinflammatory cytokines and cytokine-induced apoptosis. In this study, we aimed to investigate the effectiveness of erythropoietin in reducing the severity of bacterial translocation and inflammatory response after intestinal obstruction and the relation between asymmetric dimethylarginine and inflammatory markers. Methods: Forty Wistar albino rats (200-250 g) were divided into 4 groups as follows: Group 1 (Sham), only ileocaecal junction dissection; Group 2 (Erythropoietin), ileocaecal junction dissection and 3000 IU/kg erythropoietin subcutaneously; Group 3 (Intestinal Obstruction), complete ileal ligation; Group 4 (Intestinal Obstruction + Erythropoietin), complete ileal ligation and 3000 IU/kg erythropoietin subcutaneously. After 24 hours, the rats were sacrificed by taking blood from the heart for biochemical analyses. Peritoneal swab culture, liver, mesenteric lymph nodes, spleen and ileum were collected for microbiological and histopathological examinations. Results: Erythropoietin reduced the secretion of inflammatory cytokines, oxidative damage and bacterial translocation, prevented the formation of inflammatory changes in the intestine, liver, spleen and mesenteric lymph nodes, and also significantly prevented the formation of intestinal damage after intestinal obstruction (p<0.05). Conclusions: Asymmetric dimethylarginine levels did not differ between the groups. Erythropoietin may be useful to preserve from intestinal injury and related sepsis in patients with intestinal obstruction. Asymmetric dimethylarginine is not a suitable prognostic marker.Öğe Effects of Storage Temperature and Time on Stability of Serum Tacrolimus and Cyclosporine A Levels in Whole Blood by LC-MS/MS(Hindawi Ltd, 2015) Kaplan, Ibrahim; Yuksel, Hatice; Evliyaoglu, Osman; Basarali, M. Kemal; Toprak, Gulten; Colpan, Leyla; Sen, VelatTacrolimus and cyclosporine A are immunosuppressant drugs with narrow therapeutic windows. The aim of this study was to investigate the stability of tacrolimus and cyclosporin A levels in whole blood samples under different storage conditions. Whole blood samples were obtained from 15 patients receiving tacrolimus and 15 patients receiving cyclosporine A. Samples were immediately analyzed and then stored at different conditions (room temperature (24 degrees C-26 degrees C) for 24 hours, + 4 degrees C for 24 and 48 hours, and -20 degrees C for one month) and then analyzed again. For tacrolimus, there was a significant difference between samples analyzed immediately and those kept 24 hours at room temperature (P = 0.005) (percent change 32.89%). However, there were no significant differences between the other groups. For cyclosporine A, there was a significant difference between samples analyzed immediately and those kept 24 hours (P = 0.003) (percent change 19.47%) and 48 hours (P = 0.002) (percent change 15.38%) at + 4 degrees C and those kept 24 hours at room temperature (P = 0.011) (percent change 9.71%). Samples of tacrolimus should be analyzed immediately or stored at either + 4 degrees C or -20 degrees C, while samples of cyclosporine A should be analyzed immediately or stored at -20 degrees C.Öğe Expression of interleukin-17 in lesions of erythema multiforme may indicate a role for T helper 17 cells(Termedia Publishing House Ltd, 2014) Akkurt, Zeynep Meltem; Ucmak, Derya; Turkcu, Gul; Yuksel, Hatice; Yildiz, Kenan; Arica, MustafaIntroduction: The aim of this study was to evaluate levels of interleukin (IL)-2, IL-6, IL-8, IL-10, IL-17A and interferon gamma (IFN-gamma) in the serum of patients with erythema multiforme (EM) and to search for the presence of IL-17-expressing cells in lesional samples of EM. Material and methods: A total of 32 patients (22 females and 10 males) diagnosed with EM of the minor or major type were included in the study. Levels of IL-2, IL-6, IL-8, IL-10, IL-17A and IFN-gamma in the serum were determined and compared with healthy controls. Biopsy specimens were stained with haematoxylin and eosin (HE) and monoclonal antibodies to CD4, CD8 and IL-17 for immunohistochemical examination. Results: IL-2, 6, 8 and 17A were significantly higher in the patient group (p = 0.016, p = 0.001, p = 0.004, p = 0.006, respectively) and levels of IL-10 were significantly lower than in the control group (p = 0.046). The cellular infiltrate in lesions of EM was composed mainly of CD4+ T lymphocytes. The presence of IL-17-expressing cells, at proportion of 5 to 50%, was observed in the infiltrate. Conclusions: The demonstration of IL-17-expressing cells in lesions of EM in this study has brought forth the assumption that Th17 cells may be involved in the pathogenesis of EM.Öğe Honokiol Decreases intra-Abdominal Adhesion Formation in a Rat Model(Karger, 2015) Agacayak, Elif; Tunc, Senem Yaman; Icen, Mehmet Sait; Alabalik, Ulas; Findik, Fatih Mehmet; Yuksel, Hatice; Gul, TalipAim: The purpose of this study was to investigate the effectiveness of honokiol, a natural molecule that was shown to have antioxidant effects, in prevention of intra-abdominal adhesion formation in a rat model. Material and Method: This study was conducted on a total of 40 non-pregnant Sprague-Dawley rats, which were divided into 4 groups as follows: sham, control, saline, and honokiol groups. Both uterine horns of the rats in control, saline, and honokiol groups were exposed and a 2-cm segment of the anti-mesenteric surface of both uterine horns was traumatized by a scalpel. The saline group was administered 2 ml of saline/day intraperitoneally for 5 days after the operation. The honokiol group, on the other hand, was administered honokiol intraperitoneally at a dose of 1 mg/kg/day for 5 days after the operation. On postoperative day 14, 3 ml of intracardiac blood sample was taken from the rats for biochemical analyses, and the rats were sacrificed this way. Results: Adhesion and inflammation scores were significantly lower in the honokiol group compared with the saline and control groups (p < 0.008). Similarly, fibrosis score was significantly lower in the honokiol group compared with the saline group (p < 0.008). Conclusion: Honokiol was found to be effective in prevention of intra-abdominal adhesion formation in a rat model. However, larger studies are needed to shed light on the exact role of honokiol in intra-abdominal adhesion formation and to determine the molecular aspects of the promising results found in this study. (C) 2015 S. Karger AG, BaselÖğe Investigation of the Effects of Leptin on the Cardiovascular System in Patients With Systemic Sclerosis(Baycinar Medical Publ-Baycinar Tibbi Yayincilik, 2013) Atilgan, Zuhal Ariturk; Islamoglu, Yahya; Tekbas, Ebru; Budulgan, Mahmut; Batmaz, Ibrahim; Tahtasiz, Mehmet; Yuksel, HaticeObjective: Cardiac involvement in systemic sclerosis can be seen, and often remains silent. Leptin, which is often associated with hypertension and regulation of sympathetic tone, has been reported to contribute to the development of atherosclerosis, endothelial dysfunction and thrombosis by acting directly. The aim of this study was to investigate the effects of leptin on the cardiovascular system in patients with systemic sclerosis. Materials and Methods: Twenty-seven patients with systemic sclerosis and 28 healthy subjects as a control group were included in the study. Clinical and laboratory parameters, 24-hour Holter ECG and ambulatory blood pressure monitorings were recorded and serum leptin levels were measured in all subjects. Results: Mean leptin levels were lower in patients with systemic sclerosis than in controls. In 24-hour ambulatory blood pressure Holter recordings, the mean systolic and diastolic blood pressure, daytime systolic and diastolic blood pressure, systolic and diastolic blood pressure values at night were lower in patients when compared with controls. There was no relationship between leptin and heart rate variability parameters. A positive correlation was found between leptin levels and 24-hour ambulatory blood pressure parameters. Conclusion: Decreased serum leptin levels and increased mean heart rate in patients with systemic sclerosis may be the result of increased sympathetic tone.Öğe Investigation of Total Oxidants/Antioxidants in Patients with Intracerebral Haemorrhage(Turkish Neurological Soc, 2013) Cevik, Mehmet Ugur; Acar, Abdullah; Yucel, Yavuz; Varol, Sefer; Akil, Esref; Arikanoglu, Adalet; Yuksel, HaticeObjective: Although there are numerous studies about oxidants and antioxidants in patients with ischemic stroke, the number of studies on this subject in patients with intracerebral hemorrhage (ICH) is limited. Malondialdehyde (MDA) is an oxidant parameter investigated in patients with ICH, and total oxidant status (TOS) has not been investigated so far. We aimed to investigate in blood samples the oxidant parameters MDA and TOS, and the total antioxidant status (TAS) in patients with ICH. Material and Method: A total of 30 patients with ICH, admitted and treated at the Neurology Clinic in the Faculty of Medicine, University of Dicle and 30 control who had no stroke or any systemic disorders were included in the study. Peripheral vein blood samples taken from patients and controls were included in the first 24 hours after stroke. Serum TAS, TOS values were measured with the Erel method, a specific, fully automatic and colorimetric method, and serum level of MDA was measured with method of Ohkawa. Results: Compared to the control group, the serum levels of TAS, TOS and MDA were significantly higher in the ICH patients (p < 0.05). However, there was no correlation between serum TOS, TAS and MDA levels and Glasgow Coma Scale (GCS) total scores and hematoma volumes (p> 0.05). Discussion: The increase in the serum levels of MDA, TOS, and TAS in ICH patients may demonstrate that there is an increase in oxidative stress and this supports the fact that that oxidative stress may play a significant role in the pathogenesis of the ICH. However, the increase of these parameters was not found to be associated with hematoma volume and GCS in patients with ICH.Öğe Is montelukast as effective as N-acetylcysteine in hepatic injury due to acetaminophen intoxication in rats?(Elsevier Gmbh, 2016) Icer, Mustafa; Zengin, Yilmaz; Gunduz, Ercan; Dursun, Recep; Durgun, Hasan Mansur; Turkcu, Gul; Yuksel, HaticeThis study aims to investigate the acute protective effect of montelukast sodium in hepatic injury secondary to acetaminophen (APAP) intoxication. This study used 60 rats. The rats were grouped into 6 groups. The control group was administered oral distilled water 10 ml/kg, the APAP group oral APAP 1 g/kg, the montelukast sodium (MM) group oral MK 30 mg/kg, the acetaminophen + N-acetylcysteine (APAP + NAC) group oral APAP 1 g/kg, followed by a single dose of intraperitoneal NAC 1.5 g/kg three hours later, the acetaminophen + montelukast sodium (APAP+MK) group oral APAP 1 g/kg, followed by oral MK 30 mg/kg 3 h later, the acetaminophen + Nacetylcysteine + montelukast sodium (APAP + NAC + MM) group oral APAP 1 g/kg, followed by a single intraperitoneal NAC 1.5 g/kg plus oral MK 30 mg/kg 3 h later. Blood and liver tissue samples were taken 24 h after drug administration. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and total bilirubin were studied from the blood samples. Liver tissue samples were used for histopathological examination. Compared with the control group, serum AST and ALT activities were higher in the APAP and APAP + NAC groups. APAP + NAC, APAP + MK, and APAP + NAC + MM groups had reduced serum ALT and AST activities than the group administered APAP alone. APAP + MM and APAP + NAC + MK groups had a lower serum ALP activity than the control group. Histopathologically, there was a difference between the group administered APAP alone and the APAP + MK and APAP + NAC + MK groups. MK is as protective as NAC in liver tissue in APAP intoxication in rats. (C) 2015 Elsevier GmbH. All rights reserved.Öğe Low fetuin-A level in migraine: a case-control study(Springer-Verlag Italia Srl, 2014) Tanriverdi, Mehmet Halis; Varol, Sefer; Arikanoglu, Adalet; Bucaktepe, Pakize Gamze Erten; Celepkolu, Tahsin; Akil, Esref; Yuksel, HaticeMigraine is a type of primary headache which is caused by the alterations in trigeminovascular system. Migraine attacks are associated with neurovascular inflammation of the cerebral and extracerebral vessels, but its pathophysiological mechanisms have not still been fully delineated. Also, migraine has been found to be associated with higher risks for various metabolic disorders. Thus, we aimed to investigate the matrix metalloproteinases (MMP), fetuin-A, ghrelin, and omentin levels which have important roles in metabolic disorders and inflammation, and to examine their relationship with migraine subtypes and attack frequency. Forty-nine migraine patients and 30 age- and sex-matched healthy control subjects were enrolled. Migraine diagnosis was confirmed according to the International Classification of Headache Disorders-II diagnostic criteria. Analyses of MMP9,MMP3, ghrelin, omentin, and fetuin-A were performed by the ELISA method. Fetuin-A, MMP-9, and MMP-3 levels were significantly lower in migraine than controls (p < 0.05). There were no significant differences between groups with respect to omentin and ghrelin (p > 0.05). In migraine patients, serum fetuin-A levels were positively correlated with MMP-9 and negatively correlated with MMP-3. MMP-3, MMP-9, fetuin-A, omentin and ghrelin levels did not correlate with age, disease duration, or frequency of migraine headache (p > 0.05). Migraine patients have lower fetuin-A, MMP-3 and MMP-9 levels than healthy individuals. Migraine patients have low fetuin-A levels, which may be related to the pathogenesis of migraine. The importance and impact of our findings on the pathogenesis, characteristics, and treatment of migraine needs to be investigated in further detailed studies.Öğe Mean platelet volume in pseudoexfoliation syndrome and glaucoma(Sage Publications Ltd, 2014) Turkcu, Fatih Mehmet; Yuksel, Harun; Sahin, Alparslan; Cinar, Yasin; Yuksel, Hatice; Cingu, Kursat; Sahin, MuhammedPurpose: Pseudoexfoliation (PEX) syndrome (PES) is characterized by the widespread deposition of abnormal extracellular fibrillary material on many ocular and extraocular tissues. The objective of this study was to evaluate the association among PES, PEX glaucoma (PEG), and mean platelet volume (MPV). Methods: Forty patients with PES (mean age 62.6 +/- 7.8 years), 31 with PEG (mean age 65.9 +/- 6.6 years), and 37 healthy individuals (control group) (mean age 64.0 +/- 7.1 years) were included in the study. The MPV of the 3 groups were compared. Results: Age and sex distribution were similar among groups (p>0.05). Mean MPV in PES, PEG, and control groups were 9.59 +/- 0.94 fl, 9.53 +/- 0.80 fl, and 7.7 +/- 0.67 fl, respectively. In the PEX group, MPV values were significantly higher than in the control group (p<0.05). However, there was no difference between the PES and PEG groups (p>0.05). Conclusions: The MPV values in both groups with PEX were higher than those in the healthy group.Öğe New hormones to predict the severity of gallstone-induced acute pancreatitis(Aves, 2014) Ulger, Burak Veli; Gul, Mesut; Uslukaya, Omer; Oguz, Abdullah; Bozdag, Zubeyir; Yuksel, Hatice; Boyuk, AbdullahBackground/Aims: Levels of the hormones ghrelin and leptin in rat models of acute pancreatitis (AP) have been investigated in several experimental studies. However, there are very few clinical studies addressing the connection between hormone levels and AP. A few recent studies investigating the changes in ghrelin and leptin levels in patients with AP have been reported; however, our study is the first clinical study to investigate the change of nesfatin-1 levels in patients with gallstone-induced AP. Materials and Methods: Forty patients were enrolled in this study, eight of which presented with severe AP. Two blood samples were obtained from each study patient. The first blood samples were obtained at patient admission to the hospital and the second was obtained at patient discharge. All samples were collected after at least 6 h of fasting. Plasma nesfatin-1, leptin, and ghrelin levels were measured. Results: In all 40 patients, nesfatin-1 and leptin levels were higher at admission and had decreased at discharge. In contrast, the ghrelin levels at discharge were significantly higher than those at admission. Only the changes in these hormones in the mild AP group were significant. Conclusion: Levels of these hormones were altered during the course of gallstone-induced AP. These changes might be associated with the clinical outcomes of the disease. To clarify whether the magnitude of the change in hormone levels at AP onset can be used as a biomarkers to predict the severity of the disease requires further investigation.Öğe Protective Effect of Caffeic Acid Phenethyl Ester on Antituberculosis Drug-Induced Hepatotoxicity in Rats(Int College Of Surgeons, 2017) Aliosmanoglu, Cigdem; Erbi, Halil; Aliosmanoglu, Ibrahim; Turkoglu, Mehmet Akif; Ulger, Burak Veli; Turkoglu, Ahmet; Yuksel, HaticeIsoniazid and rifampicin are drugs primarily used in antituberculosis treatment. Our aim in this study is to evaluate the effect of caffeic acid phenethyl ester's protective effect on liver function tests and to trace elements in hepatic damage caused by isoniazid and rifampicin on rats. Forty Wistar albino rats were divided into 4 groups. Group 1: Sham, Group 2: caffeic acid phenethyl ester application, Group 3: isoniazid and rifampicin given, Group 4: isoniazid + rifampicin and caffeic acid phenethyl ester application. After 30 days, the rats were sacrificed by taking blood from the heart. Alanine aminotransferase, aspartate aminotransferase, zinc, copper, total antioxidant capacity, total oxidative status, and oxidative stress index levels were evaluated. The rats to which isoniazid + rifampicin + caffeic acid phenethyl ester were given had less oxidative stress and copper levels (P < 0.001, P = 0.019) but have higher zinc levels (P = 0.001) compared to the isoniazid + rifampicin group. Liver enzyme levels were also lower in rats that were given isoniazid + rifampicin + caffeic acid phenethyl ester (P < 0.001). The results of this study suggested that caffeic acid phenethyl ester influences the levels of trace elements (copper and zinc) that are important for the physiologic mechanisms of organisms, reducing liver damage.Öğe The Protective Effect of Ellagic Acid Against Renal Ischemia-Reperfusion Injury in Male Rats(Kafkas Univ, Veteriner Fakultesi Dergisi, 2012) Bozkurt, Yasar; Firat, Ugur; Atar, Murat; Sancaktutar, Ahmet Ali; Pembegul, Necmettin; Soylemez, Haluk; Yuksel, HaticeThe aim of this study was to evaluate the possible protective effect of ellagic acid (EA) on rats following renal ischemia reperfusion (I/R) injury. Twenty-four Wistar rats were divided into three groups. Sham group underwent laparotomy then waited for 45 min without ischemia. I/R group were subjected to left renal ischemia for 45 minutes followed by 60 min of reperfusion. I/R+EA group were subjected to the same renal ischemia/reperfusion as the I/R group, were also given 85 mg/kg EA perorally 30 min prior to the ischemia. Malondialdehyde (MDA), total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) were determined on the blood samples and kidney tissues. Histopathological analyses were conducted on the kidney tissues. I/R damage significantly increased serum MDA levels in the I/R group when compared with Sham group. Serum TAC level was significantly lower in I/R group than I/R+EA group. A significantly increase on OSI levels and decrease on TAC levels was found in the kidneys in I/R group. In I/R + EA group, EA reversed the negative effects of I/R injury. EA pretreatment was effective in decreasing tubular necrosis score. In conclusion; EA pretreatment ameliorated the oxidative damage and histopathological changes occurring following renal I/R injury.Öğe The protective effects of curcumin on intestine and remote organs against mesenteric ischemia/reperfusion injury(Aves, 2012) Onder, Akin; Kapan, Murat; Gumus, Metehan; Yuksel, Hatice; Boyuk, Abdullah; Alp, Harun; Basarili, Mustafa KemalBackground/aims: Mesenteric ischemia / reperfusion injury induces a systemic response and releases harmful substances that may affect distant organs such as the lung, liver and kidney. We designed this study to determine if curcumin ha.; protective effects against mesenteric ischemia / reperfusion injury and mesenteric ischemia / reperfusion-induced intestinal and distant organ injury. Methods: Forty Wistar-Albino rats were divided into four groups as: sham, control, ischemia / reperfusion, and ischemia / reperfusion+curcumin. The ischemia / reperfusion and ischemia / reperfusion+curcumin groups were subjected to mesenteric arterial ischemia for 30 minutes and reperfusion for 1 hour. The control and ischemia 1 reperfusion+curcumin groups were administered curcumin (200 mg / kg, single dose) via oral gavage 15 min before the injury insult. Blood and pulmonary, hepatic and kidney tissue specimens were obtained to measure serum malondialdehyde and total antioxidant capacity, tissue levels of total antioxidant capacity, total oxidative status, and oxidative stress index. In addition, intestine, pulmonary, hepatic, and kidney tissue specimens were obtained for the evaluation of histopathological changes. Results: The histopathological injury scores of the intestine and distant organs were significantly higher in the ischemia / reperfusion group; these injuries were prevented by curcumin in the ischemia / reperfusion+curcumin group. In the ischemia / reperfusion group, a significant increase in serum malondialdehyde levels was determined, which was prevented with curcumin pretreatment in the ischemia / reperfusion+curcumin group. Total antioxidant capacity levels were significantly supported by curcumin pretreatment in the control and ischemia / reperfusion+curcumin groups. Conclusions: This study demonstrated that curcumin ameliorates histopathological damage in the intestine and distant organs against mesenteric ischemia / reperfusion injury.
