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    Biochemical, Histopathologic, and Genotoxic Effects of Ethanol Extract of Salvia hypargeia (Fisch. & Mey.) on Incisional and Excisional Wounded Diabetic Rats
    (Taylor & Francis Inc, 2021) Ozay, Yusuf; Guzel, Sevda; Ozkorkmaz, Ebru Gokalp; Kumas, Meltem; Uzun, Cosar; Yildirim, Zuhal; Celik, Ayla
    Purpose: Nonhealing wounds are a serious problem of diabetic patients. Salvia species are traditionally used for the treatment of wounds. The aim of the study was to investigate the effects of ointment prepared with ethanol extract obtained from the aerial parts of Salvia hypargeia, an endemic plant from Turkey, on diabetic rat incisional and excisional skin wounds. Materials and Methods: Male Wistar albino rats (n: 60) were divided into five groups. Diabetes was induced and two concentrations (0.5% and 1%) of the extract were used for ointments and applied on wounds for 7 and 14 days. Fito cream was chosen as a reference drug. Results: In excisional wounds, healing ratios of 0.5% (63.4% and 99.3%) and 1% (65.5% and 99.9%) S. hypargeia groups were higher compared to control (35.9% and 75.1%), and in incisional wounds, healing ratios of 0.5% (78.1% and 98.5%) and 1% (84.4% and 99.4%) S. hypargeia groups were higher compared to control (30.5% and 72.9%) (p < .01). Hydroxyproline (0.31 +/- 0.3 and 0.34 +/- 0.2) levels were lower and GSH (10.7 +/- 3.1 and 7.6 +/- 0.9) levels were higher in 0.5% and 1% S. hypargeia groups on the 14th day (p < .01). Histopathological results revealed re-epithelialization and formation of granulation tissue in all S. hypargeia groups. Genotoxicologic results indicated, GDI, DCP values, and MN frequency of 0.5% and 1% S. hypargeia groups did not reach to significant levels both on the 7 and 14 days. Conclusions: S. hypargeia may have a potential for therapeutic use in treatment and management of diabetic wounds with a successful topical application.
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    Öğe
    Effects of methanolic extract of Verbascum inulifolium Hub.-Mor. on incisional and excisional skin wounds in diabetic and non-diabetic rats
    (Natl Inst Science Communication-Niscair, 2019) Ozay, Yusuf; Guzel, Sevda; Ozkorkmaz, Ebru Gokalp; Yildiz, Atilla; Yildirim, Zuhal; Erdogdu, Ibrahim Halil; Darcan, Sinan
    The genus Verbascum, commonly known as mullein, traditionally used in folk medicine, is known for its anti-inflammatory, antioxidant and antibacterial activities. Verbascum inulifolium Hub.-Mor. (Scrophulariaceae), is an endemic species from Turkey. Here, we examined the healing effect of methanol extract of the aerial parts of V. inulifolium on incisional and excisional skin wounds in diabetic and non-diabetic rats. Ointments of the extract were prepared in two concentrations (0.5 and 1% (w/w)) and applied topically on wound models once daily throughout for 7 and 14 days. During the experiments, wounds were visually observed, photographically documented and wound areas were measured. After 7 and 14 days treatments, animals were sacrificed and measurements of hydroxyproline level and biomechanical analysis were performed. Histopathology of the wound area was evaluated considering features of re-epithelialization, the granulation tissue thickness, angiogenesis, presence of inflammation, number of mast cells. Outcomes of this study revealed that the ethanolic extract of the aerial parts of V. inulifolium enhances the healing process of skin tissue in both non-diabetic and diabetic wounds. The best wound healing activity was observed in incisional wound treated with 0.5% (w/w) concentrated ointment (99.7%) and in excisional wound treated with 1% (w/w) concentrated ointment (98.1%) on day 14 according to macroscopic results.
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    Öğe
    Wound healing activity of Salvia huberi ethanolic extract in streptozocininduced diabetic rats
    (Ma Healthcare Ltd, 2023) Ozay, Yusuf; Ozkorkmaz, Ebru Gokalp; Kumas-Kulualp, Meltem; Kara, Sevda Guzel; Yildirim, Zuhal; Uzun, Cosar; Celik, Ayla
    Objective: The aim of this study was to examine the in vivo wound healing potential of Salvia huberi Hedge (endemic to Turkey) on excision and incision wound models in diabetic rats. Method: Male Wistar albino rats, 3-4 months old and weighing 180-240g were used. The animals were randomly divided into five groups including Control, Vehicle and Fito reference, and two different concentrations (0.5% and 1% weight/weight (w/w)) of ethanol extract of Salvia huberi were investigated in both wound models on streptozocin-induced diabetic rats using macroscopic, biomechanical, biochemical, histopathological, genotoxic and gene expression methods over both seven and 14 days. Fito cream (Tripharma Drug Industry and Trade Inc., Turkey) was used as the reference drug. Results: A total of 60 rats were used in this study. Salvia huberi ointments at 0.5% and 1% (w/w) concentrations and Fito cream showed 99.3%, 99.4% and 99.1% contraction for excision wounds, and 99.9%, 97.0% and 99% contraction for incision wounds, respectively. In Salvia huberi ointments and Fito cream groups, re-epithelialisation increased dramatically by both day 7 and day 14 (p<0.05). By day 14, low hydroxyproline and malondialdehyde (MDA) levels, and high glutathione (GSH) levels were observed in the Salvia huberi ointment groups. After two application periods, damaged cell percent and genetic damage index values and micronucleus frequency of Salvia huberi ointment treatment groups were lower than Control and Vehicle groups (p<0.001). A growth factor expression reached a high level by day 7 in the Control group; in Salvia huberi-treated groups it was decreased. Conclusion: The study showed that application of Salvia huberi ointments ameliorated the healing process in diabetic rats with excisional and incisional wounds and may serve as a potent healing agent. Declaration of interest: The authors have no conflicts of interest.