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Öğe Comparison of Apical Microleakage of Dual-Curing Resin Cements with Fluid-Filtration and Dye Extraction Techniques(Int Scientific Literature, Inc, 2015) Kaya, Sadullah; Ozer, Senem Yigit; Adiguzel, Ozkan; Orucoglu, Hasan; Deger, Yalcin; Tumen, Emin Caner; Uysal, IbrahimBackground: Endodontically treated teeth with excessive loss of tooth structure are frequently restored using fiber posts. In this in vitro study, the apical leakage of self-and dual-activated curing modes for dual-curing resins cementing a translucent fiber post was evaluated using computerized fluid filtration meter and dye extraction method. Material/Methods: One hundred and four extracted human maxillary incisors with single root and canal were used. Experimental samples embedded in a closed system were divided into 4 groups (n=20) according to 2 dual-curing luting systems, with 2 different curing modes (either with self-or light-activation): (1) Panavia F 2.0 with self-cure, (2) Panavia F 2.0 with light-activation, (3) Clearfill SA with self-cure, and (4) Clearfill SA with light activation. Twenty-four teeth served as negative and positive controls. Translucent fiber posts were luted in the roots except in the control groups. Results: Statistical analysis indicated no significant difference in leakage among groups (p>0.05) with 4.12x10(-4) (Panavia self-cure), 4.55x10(-4) (Clearfill SA self-cure), 5.17x10(-4) (Panavia dual-cure), and 5.59x10(-4) (Clearfill SA dual-cure) in fluid-filtration method. Absorbance values for dye-extraction method were 266 nanometer (nm) (Panavia self-cure), 268 nm (Clearfill SA self-cure), 270 nm (Panavia dual-cure), and 271 nm (Clearfill SA dual-cure), in which difference among the groups were not statistically significant (p>0.05). When comparing the leakage, assessment methods results showed no statistically significant difference between the tested evaluation techniques (p>0.05). Conclusions: Light-and self-activation curing modes of Panavia F 2.0 and Clearfill SA perform similar to each other in a closed system.Öğe Effect of Irrigation Solution Temperature on Bioceramic Sealer Bond Strength(Int Scientific Information, Inc, 2025) Kilivan, Hasret; Uysal, IbrahimBackground: Different temperature conditions can affect the efficiency of irrigation solutions and consequently the ability of canal sealers to bond to root canal walls. The aim of this endodontic study was to evaluate the effect of irrigation solutions at different temperatures on the bond strength of a bioceramic-based root canal sealer. Material/Methods: Root canal preparations were completed through irrigation with the following solutions: Group 1 was irrigated with 5 ml NaOCl (sodium hypochlorite) +5 ml EDTA (Ethylenediamine tetra-acetic acid) (22 degrees C); Group 2 was irrigated with 5 ml NaOCl +5 ml EDTA (37 degrees C); Group 3 was irrigated with 5 ml NaOCl +5 ml GA (Glycolic acid) (22 degrees C); Group 4 was irrigated with 5 ml NaOCl +5 ml GA (37 degrees C), Group 5 was irrigated with 20 ml Dual Rinse (R) HEDP (Etidronate) - NaOCl mixture (22 degrees C); and Group 6 was irrigated with 20 ml of Dual Rinse (R) HEDP mixture (37 degrees C). Obtained test specimens were subjected to the push-out test. Three-way ANOVA was used to compare bond strength values. Results: The main effect of temperature (P<0.05), the main effect of the solution (P<0.05), and the main effect of the section (P<0.05) were significantly associated with the mean values of the bond strength. Heating irrigation solutions increased the bond strength of bioceramic-based canal sealers. Conclusions: The bond strengths of the solutions increased as the temperature increased. EDTA solution significantly increased the bond strength compared to Dual Rinse (R) HEDP solution. Meanwhile, the bond strengths in the apical region were lower than those in the middle region.Öğe Effectiveness of ethylenediaminetetraacetic acid (EDTA) and MTAD on debris and smear layer removal using a self-adjusting file(Mosby-Elsevier, 2011) Adiguzel, Ozkan; Yigit-Ozer, Senem; Kaya, Sadullah; Uysal, Ibrahim; Ganidagli-Ayaz, Selengul; Akkus, ZekiObjective. The aim of this study was to investigate the cleaning ability of a self-adjusting file (SAF) system regarding debris and smear layer removal using ethylenediaminetetraacetic acid (EDTA) or MTAD. Study design. In total, 45 maxillary incisor teeth were randomly divided into 2 different irrigation groups of 20 canals each and a negative control group of 5 canals. The canals in each of the irrigation groups were irrigated using sodium hypochlorite (1.3%) as an initial irrigant during the first 2 minutes of operation, followed by 2 minutes continuous irrigation with either 17% EDTA or MTAD in a closed system. The negative control group was irrigated using 1.3% sodium hypochlorite. The roots were split longitudinally and subjected to scanning electron microscopy (SEM). The presence of debris and smear layer in the coronal, middle, and apical thirds of the canal was evaluated using a 5-grade scoring system with x200 and x2,000 magnification, respectively. Results. The SAF operation with 2-minute continuous irrigation using MTAD resulted in root canal walls that were free of smear layer in 85%, 70%, and 60% and of debris in 95%, 90%, and 95% of the coronal, middle, and apical thirds of the root canals, respectively. The SAF operation with continuous irrigation using EDTA resulted in root canal walls that were free of smear layer in 85%, 60%, and 50% and of debris in 95%, 90%, and 85% of the coronal, middle, and apical thirds of the root canals, respectively. Teeth in the negative control group were totally covered with debris. Evaluation by SEM showed no significant difference between the tested irrigants in removing the smear layer and debris among the different regions of the root canal. Both groups were significantly different from the negative control group. Conclusions. When using the SAF, the protocols used in this study were effective for debridement for all regions of the root canal even for the apical thirds. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011;112:803-808)Öğe Effects of Nicotine on Rat Alveolar Bone(Sci Printers & Publ Inc, 2016) Eratilla, Veysel; Uysal, Ibrahim; Ozevren, Huseyin; Uysal, Rojdan Ferman Gunes; Aktas, Elif Eratilla; Karabulut, Ozlen; Deveci, EnginOBJECTIVE: To investigate the effects of nicotine on periodontal membranes and alveolar bone using histopathological and immunohistochemical methods. STUDY DESIGN: Rats were divided into 2 groups: those systemically treated with nicotine sulfate (2 mg/kg subcutaneously, daily) for 28 days, and untreated controls. We analyzed the distribution of osteonectin and osteopontin immunohistochemically, with antibodies, in the maxillary region. RESULTS: In the treatment group, resorption of alveolar bone and vascular dilation, bleeding, periodontal membrane degeneration, hyalinization, and necrosis were seen in some areas. Negative osteoblastic activity was observed in immunohistochemical analyses of collagen fibers in the periodontal membrane. CONCLUSION: In short- and long-term nicotine consumption, soft tissue and alveolar bone are negatively affected during cell proliferation. This phenomenon may lead to a delay in treatment of implantation.Öğe Effects of Nicotine on Rat Tongue Muc Histopathological and Immunohistochemical Analysis(Sci Printers & Publ Inc, 2016) Eratilla, Veysel; Uysal, Ibrahim; Deveci, Engin; Gunes, Rojdan Ferman; Eratilla, Elif; Yildiz, Ismail; Soker, SevdaOBJECTIVE: To evaluate the histopathological and immunohistochemical effects of systemically administered nicotine on rat tongue mucosa. STUDY DESIGN: Rats were assigned to groups: the experimental group received nicotine ically (nicotine sulphate 2 mg/kg subcutaneously for 28 days), while the rats in the trol grow ere administered physiological saline mL subcutaneously for 28 days). All animals sacrificed at the end of the study, and tongue tissue ere removed and prepared according his histological procedures. Sections were sta toxylin a sin and observed by. Immunorec y of tongue mucosa , 'E-ca Ilagen IV, and VEr by immunhistochemical staining. RESULTS: There w if cant differences in the average histlogical a between the nicotine-treated treated gr Morphological changes, including inflammatory I to infiltration and cellular desquamation, blood dilation, hemorrhage, and epithelial degeneration r. re noted. Further, E-cadherin expression w significantly decreased in the nicotine-treated grow,w the untreated group. The nicotine treat up showed an increase in collagen IV secondary ae and basal cells. CONCLUSION: The increased level of VEGF exprescs n in the nicotine-treated group may have affected endothelial cell apoptosis.Öğe Effects of Sildenafil on Dental Pulp: Immunohistochemical and Ultrastructural Evaluation(Soc Chilena Anatomia, 2016) Soker, Sevda; Deveci, Engin; Yaman, Ferhan; Atilgan, Serhat; Ipek, Fikret; Uysal, IbrahimSildenafil is a strong peripheral vasodilator and is used to treat cardiovascular and neurosurgery. The purpose of this study was to investigate the immunohistochemical and ultrastructural effects of sildenafil on dental pulp of rats. The study was performed with adult female Wistar-Albino rats. Control group (n=7) were fed on standard laboratory diet until surgery. The study group (n=7) were administered sildenafil orally with orogastric tube 10 mg.kg(-1) once a day for 30 days. Each rat was anesthetized and incisor teeth were removed. This study examined the immunohistochemical and ultrastructural effects of sildenafil on the dental pulp in rats. The relaxation from the vessel, endothelial cell hyperplasia, moderate degeneration of collagen fibers were observed to cause degenerative changes in odontoblast with sildenafil. In the pulp tissue long-term use sildenafil is thought to cause degeneration and new vessel formation.Öğe Experimentally Induced Diabetes Mellitus Influences Expression of VEGF and CD68 in Rat Teeth Pulp(Soc Chilena Anatomia, 2019) Uysal, Ibrahim; Gokalp-Ozkorkmaz, Ebru; Deveci, EnginThe purpose of this study was to examine the expression levels of the dental pulp to elucidate the role of Vascular Endothelial Growth Factor (VEGF) and CD68 on vascular angiogenesis, inflammation and odontoblast differentiation in the pulp tissue of diabetic rats depending on the effect of possible damage induced by diabetes. Wistar rats were used in the study, divided into two groups. Control group was fed with standard rat chow and drinking water ad libitum for 8 weeks. Single dose of streptozotocin (STZ) (55 mg/kg), was disolved in sodium citrate buffer and administered by intraperitoneal injection. Blood glucose concentration of rats exceeding 250 mg/dl were accepted as diabetic. Rats were sacrificed under anesthesia. Tissues were immediately dissected, fixed and embedded in paraffin and cut with a microtome then examined under light microscope. In the cross-sections of pulp tissue of diabetic group; the dilation of blood vessels besides hemorrhage and a significant increase in inflammatory cells were seen. The expression of VEGF in the blood vessel endothelial cells of the pulp was increased. VEGF showed positive reaction for degenerative odontoblast cells in the pulp. In this study, increase in VEGF and CD68 expressions in pulp tissue due to the effect of diabetes was thought to delay pulp treatment by inducing soft tissue damage and hypoxia.Öğe Measuring Bone Density in Healing Periapical Lesions by Using Cone Beam Computed Tomography: A Clinical Investigation(Elsevier Science Inc, 2012) Kaya, Sadullah; Yavuz, Izzet; Uysal, Ibrahim; Akkus, ZekiIntroduction: Differences in bone density before and after endodontic treatment were examined in teeth with periapical lesions in Hounsfield units (HUs) by using cone beam computed tomography (CBCT). Methods: Sixteen patients requiring endodontic treatment for periapical lesions underwent CBCT scan before and after treatment. Their bone densities were calculated in HUs. The study included 16 lesions measuring 8-10 mm in diameter. HU measurements were taken from an area of 2.25 mm(2) on the CBCT images where the bone density was lowest, before and after treatment. Results: The initial and final measurements were compared statistically by using paired samples statistics at the 5% significance level. Conclusions: The results of this study support the use of CBCT to measure bone density before and after endodontic treatment. (J Endod 2012;38:28-31)
