Yazar "Turak, Fatma" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • [ X ]
    Öğe
    A New UPLC Approach for the Simultaneous Quantitative Estimation of Four Compounds in a Cough Syrup Formulation
    (Oxford Univ Press Inc, 2016) Turak, Fatma; Guzel, Remziye; Dinc, Erdal
    A new ultra-performance liquid chromatographic (UPLC) method was developed for the simultaneous estimation of potassium guaiacolsulfonate (PGS), guaifenesin (GUA), diphenhydramine HCl (DIP) and carbepentane citrate (CAR) in a commercial cough syrup. The chromatographic separation of four compounds PGS, GUA, DIP and CAR was performed on a BEH phenyl column (100 x 2.1 mm, 1.7 mu m i.d.) using a mobile phase consisting of acetonitrile and 0.1 MHCl (50 : 50, v/v). In addition, the optimized conditions of the chromatographic analysis were found with the flow rate of 0.38 mL/min, the column temperature of 30 degrees C and the injection volume of 1.2 mu L with the photodiode array detection of 220 nm. Calibration curves in the concentration ranges of 10-98 mu g/mL for PGS, 5-80 mu g/mL for GUA, 5-25 mu g/mL for DIP and CAR were computed by the regression of the analyte concentration on the chromatographic peak area. The newly developed UPLC method was validated by analyzing the quaternary mixtures of the related compounds, intraday and interday experiment and standard addition samples. After method validation, the proposed UPLC approach was successfully applied for the analysis of the commercial syrup formulation containing PGS, GUA, DIP and CAR compounds.
  • [ X ]
    Öğe
    Simultaneous determination of ascorbic acid and caffeine in commercial soft drinks using reversed-phase ultraperformance liquid chromatography
    (Food & Drug Adminstration, 2017) Turak, Fatma; Guzel, Remziye; Dinc, Erdal
    A new reversed-phase ultraperformance liquid chromatography method with a photodiode array detector was developed for the quantification of ascorbic acid (AA) and caffeine (CAF) in 11 different commercial drinks consisting of one energy drink and 10 ice tea drinks. Separation of the analyzed AA and CAF with an internal standard, caffeic acid, was performed on a Waters BEH C-18 column (100 mm x 2.1 mm, 1.7 mu m i.d.), using a mobile phase consisting of acetonitrile and 0.2M H3PO4 (11: 89, v/v) with a flow rate of 0.25 mL/min and an injection volume of 1.0 mu L. Calibration graphs for AA and CAF were computed from the peak area ratio of AA/internal standard and CAF/internal standard detected at 244.0 nm and 273.6 nm, respectively. The developed reversed-phase ultraperformance liquid chromatography method was validated by analyzing standard addition samples. The proposed reversed-phase ultraperformance liquid chromatography method gave us successful results for the quantitative analysis of commercial drinks containing AA and CAF substances. Copyright (C) 2016, Food and Drug Administration, Taiwan. Published by Elsevier Taiwan LLC.