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    In vitro inhibition of advanced glycation end product formation by ethanol extract of milk thistle (Silybum marianum L.) seed
    (Elsevier, 2022) Inceoren, Nesrin; Emen, Sevil; Toptanci, Bircan Ceken; Kizil, Goksel; Kizil, Murat
    Glycation of proteins by reducing sugars produces toxic and immunogenic compounds called advanced gly-cation end products (AGEs). AGEs plays important role in the progression of typical diabetes complications. Silybum marianum L. contains powerful antioxidants that quench free radicals, which are unstable and reac-tive compounds that damage DNA and cellular structures. This study aimed to evaluate the antioxidant activ-ities and the inhibitory effects of ethanol:water (1:1) extract of the Silybum marianum seed (SlyE) and silymarin (Sly) on fructose mediated protein glycation and AGE formation. The total amount of silymarin in SlyE was found to be 2.59 mg/gDW by HPLC. The composition of phenolic in the extract was found to be 262.70 mg gallic acid (GAE)/mg extract. The composition of flavonoids in the extract was found to be 10.50 mg quercetin (QUE)/mg extract. The seed exhibited ABTS radical cation decolourization inhibition with 70.4% at 300 mg concentration. Ferric reducing power of the extract was also evaluated. The SlyE and Sly also demonstrated strong inhibitory effects on the production AGEs in bovine serum albumin (BSA)/fructose model. SlyE and Sly has a significant effect in preventing oxidative protein damage, including the effect on the formation of fructosamines, a-dicarbonyls and protein carbonyl content (PCC). SlyE and Sly were able to prevent the glycoxidative damage of BSA. It can be concluded that the SlyE has antiglycating potential as well as antioxidant properties. (C) 2022 SAAB. Published by Elsevier B.V. All rights reserved.
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    Protective activity of Hertia cheirifolia extracts against DNA damage, lipid peroxidation and protein oxidation
    (Taylor & Francis Ltd, 2017) Kada, Seoussen; Bouriche, Hamama; Senator, Abderrahmane; Demirtas, Ibrahim; Ozen, Tevfik; Toptanci, Bircan Ceken; Kizil, Goksel
    Context: Hertia cheirifolia L. (Asteraceae), a perennial shrub widely distributed in Northern Africa, is traditionally used to treat inflammatory disorders. Objective: The protective effect of methanol (Met E) and aqueous (Aq E) extracts of Hertia cheirifolia against DNA, lipid and protein oxidation was investigated. Materials and methods: Different concentrations (50-1000 mu g/mL) of Hertia cheirifolia aerial part extracts were examined against DNA, lipid and protein oxidation induced by H2O2 +UV, FeSO4, and Fe3+/H2O2-ascorbic acid, respectively. The DPPH center dot, metal ion chelating, reducing power and beta-carotene bleaching tests were conducted. Results: Both extracts were rich in polyphenols, flavonoids and tannins, and were able to scavenge DPPH center dot with IC50 values of 138 and 197 mu g/mL, respectively. At 300 mu g/mL, Aq E exerted stronger chelating effect (99%) than Met E (69%). However, Met E reducing power (IC50 = 61 mu g/mL) was more than that of Aq E (IC50 = 193 mu g/mL). Both extracts protected from beta-carotene bleaching by 74% and 94%, respectively, and inhibited linoleic acid peroxidation. The inhibitory activity of Aq E extract (64%) was twice more than that of Met E (32%). Interestingly, both extracts protected DNA against the cleavage by about 96-98%. At 1 mg/mL, Met E and Aq E restored protein band intensity by 94-99%. Conclusions: Hertia cheirifolia exhibits potent antioxidant activity and protects biomolecules against oxidative damage; hence, it may serve as potential source of natural antioxidant for pharmaceutical applications and food preservation. This is the first report on the protective activity of this plant against biomolecule oxidation.
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    PROTECTIVE EFFECT OF OKUZGOZU (VITIS VINIFERA L. CV.) SEED EXTRACT AGAINST HYDROXYL RADICAL INDUCED DNA DAMAGE
    (Univ Agricultural Sciences & Veterinary Medicine Bucharest, 2017) Pirinccioglu, Mihdiye; Kizil, Goksel; Toptanci, Bircan Ceken; Ozdemir, Gultekin; Kizil, Murat
    Grape is one of the most commonly consumed fruits in the world. It has various biological functions, due to its potential of rich polyphenol ingredients, most of which are contained in its seeds (70%) and skin (30%). The aim of this study was to investigate the protective role of Okuzgozu cv. grape seed extracts against hydroxyl radical that induced oxidative DNA damage. The results revealed that the presence of various concentrations of grape seed extract in the reaction mixture significantly inhibited DNA damage induced by reactive oxygen species (ROS). In conclusion, the results demonstrated that Okuzgozu cv. grape seed extracts protected DNA against hydroxyl radical that induced oxidative damage and the extract could be used as a valuable food supplement or a nutraceutical product.