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Öğe Aldehyde dehydrogenase genes as prospective actionable targets in acute myeloid leukemia(MDPI, 2023) Dancik, Garrett M.; Varışli, Lokman; Tolan, Veysel; Vlahopoulos, SpirosIt has been previously shown that the aldehyde dehydrogenase (ALDH) family member ALDH1A1 has a significant association with acute myeloid leukemia (AML) patient risk group classification and that AML cells lacking ALDH1A1 expression can be readily killed via chemotherapy. In the past, however, a redundancy between the activities of subgroup members of the ALDH family has hampered the search for conclusive evidence to address the role of specific ALDH genes. Here, we describe the bioinformatics evaluation of all nineteen member genes of the ALDH family as prospective actionable targets for the development of methods aimed to improve AML treatment. We implicate ALDH1A1 in the development of recurrent AML, and we show that from the nineteen members of the ALDH family, ALDH1A1 and ALDH2 have the strongest association with AML patient risk group classification. Furthermore, we discover that the sum of the expression values for RNA from the genes, ALDH1A1 and ALDH2, has a stronger association with AML patient risk group classification and survival than either one gene alone does. In conclusion, we identify ALDH1A1 and ALDH2 as prospective actionable targets for the treatment of AML in high-risk patients. Substances that inhibit both enzymatic activities constitute potentially effective pharmaceutics.Öğe Anason (Pimpinella anisum L.) ve kimyon (Cuminum cyminum L.) tohumlarının uçucu yağ kompozisyonu ile antimikrobiyal ve antioksidan özelliklerinin belirlenmesi(2014) Kızıl, Süleyman; Tolan, Veysel; Haşimi, Nesrin; Kılınç, ErsinBu çalışmada, anason (Pimpinella anisum L.) ve kimyon (Cuminum cyminum L.) tohumlarının uçucu yağ bileşenleri ile bu yağların antimikrobiyal ve antioksidan özelliklerinin belirlenmesi amaçlanmıştır. Uçucu yağ bileşenleri GC/MS cihazı ile belirlenmiştir. Yağların antimikrobiyal özellikleri disk difüzyon yöntemi ile Gram pozitif (Staphylococcus aureus ATCC 25923, Streptococcus pyogenes ATCC 19615), Gram negatif (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853) bakterileri ve maya (Candida albicans ATCC 10231) kullanılarak, antioksidan özellikler ise DPPH yöntemiyle belirlenmiştir. Anason uçucu yağının esas bileşeninin trans-anetol (% 52.94) olduğu, bunu iso-anetol (% 13.89), karyofillen oksit (% 8.55) ve karyofillen (% 4.29)’in izlediği; kimyon uçucu yağının ise sırası ile ?-pinen (% 15.77), ?- terpinen (% 15.52), 1-fenil-1-butanol (% 15.13) ve kuminik aldehit (% 12.74) içerdiği saptanmıştır. Anason uçucu yağının mikroorganizmalar üzerinde düşük, kimyon uçucu yağının ise orta derecede antimikrobiyal aktivite gösterdiği saptanmıştır. Kimyon uçucu yağı C. albicans’a karşı 22±0.9 mm’lik inhibisyon zon çapı ile yüksek antimikrobiyal aktivite göstermiştir. P. aeruginosa her iki uçucu yağa karşı direnç göstermiştir. Kimyon uçucu yağının antioksidan aktivitesi (% 75.60) askorbik asit aktivitesine (% 78.75) yakın, BHA (% 50.45) ve BHT (% 23.54) aktivitesinden daha yüksek bulunmuştur. Anason düşük antioksidan aktivite (% 23.24) göstermiş olmasına rağmen BHT ile benzer aktivite göstermiştir. Çalışmada kullanılan örneklerin aktivite sıralaması askorbik asit>kimyon>BHA>BHT>anason şeklinde gerçekleşmiştir.Öğe Bazı pestisitlerin ve Dicle Nehri yüzey suyunun genotoksik potansiyelinin kısa zamanlı bakteriyel test sistemleri ile araştırılması(2016) Tolan, Veysel; Otludil, BirolBu çalışmada, zirai mücadelede sıkça kullanılan bazı pestisitlerin ve Dicle. Nehri yüzey suyunun genotoksik potansiyelinin Ames/Salmonella/mikrozm testi ve SOS kromotest sistemi ile araştırılması amaçlanmıştır. Aynı zamanda, kimyasal bileşiklerin mutajenik ve kanserojenik etkilerinin araştırılmasında kullanılan kısa zamanlı bakteriyel test sistemlerinden olan Ames test ve SOS kromotest sistemini laboratuarımızda standardize etmek de amaçlanmıştır. Bu doğrultuda, her iki test sisteminde kullanılan mutant bakteri suşları (S.typhimurium TA98, S.typhimurium TA100 ve E.coli PQ37 suşları) temin edildikten hemen sonra taşıdıkları genetik işaretler açısından kontrol edildikten sonra bileşikler test edildi. Dicle Nehri yüzey suyu örnekleri, metal kirlenmenin daha çok olduğu düşünülen 3 istasyon belirlendikten sonra örnekler bu istasyonlardan alındı ve kimyasal analizleri ve genotoksik testleri yapıldı. Pestisitlerin ve su örneklerinin, Salmonella/mikrozom testi için sitotoksik dozları belirlendikten sonra, belirlenen dozdan düşük olmak koşulu ile, farklı konsantrasyonlar pozitif ve negatif mutajenlerle birlikte test edildi. Thiodan, S.typhimurium TA98 suşunda S9 fraksiyonu varlığında ve yokluğunda genotoksik aktivite göstermiştir. Test edilen pestisitlerden maneb ve mancozeb S.typhimurium TA98 suşunda S9 fraksiyonu yoklıuğunda genotoksik aktive göstermezken S9 fraksiyonu varlığında genotoksik aktif bulunmuştur. Bu sonuç bize her iki pestisidin de metabolik reaksiyon sonucu oluşan metabol itlerin etkili olduğunu düşündürmektedir. Dicle Nehri yüzey suyu örnekleri S.typhimurium TA98 suşunda verdikleri yanıtlar: I. İstasyon, II. İstasyon ve İÜ. İstasyon su örneklerinde S9 fraksiyonu VI yokluğunda ve S9 fraksiyonu varlığında 10, 1 ve 0.1 ul/petride pozitif yanıt gözlenmiştir. Pestisitlerden thiodan, S.typhimurium TA100 suşunda S9 fraksiyonu varlığında ve yokluğunda genotoksik aktivite göstermiştir Maneb S.typhimurium TA 100 suşunda S9 fraksiyonu yokluğunda negatif yanıt vermişken S9 fraksiyonu varlığında 8, 6 ve 4 ug/petride pozitif yanıt vermiştir. Mancozeb ise S9 fraksiyonu varlığında ve yokluğunda pozitif yanıt vermemiştir. Dicle Nehri yüzey suyuna ait örneklerin S.typhimurium TA100 suşunda verdikleri yanıtlar: I. İstasyona ait su S9 fraksiyonu yokluğunda ve varlığında negatif; II. İstasyon su örneklerinden S9 fraksiyonu yokluğunda ve varlığında 10, 1 ve 0.1 ul/petride mutajenite göstermiştir, m. İstasyon su örneklerinden S9 fraksiyonu yokluğunda ve varlığında 10, 1 ve 0.1 ul/petride mutajenik aktivite göstermiştir. SOS kromotest sistemi ile test ettiğimiz pestisitlerden maneb, mancozeb ve thiodan genotoksik aktivite göstermiştir. Test edilen Dicle Nehri yüzey suyu örneklerinden I.,II. ve m. İstasyon örnekleri SOS kromotest sisteminde genotoksik aktivite göstermemiştir.Öğe Biological Activities of Origanum, Satureja, Thymbra and Thymus Species Grown in Turkey(Taylor & Francis Ltd, 2014) Kizil, Suleyman; Hasimi, Nesrin; Tolan, VeyselIn the present study, seven thyme species Origanum onites, O. vulgare var. hirtum, O. vulgare var. gracile, O. syriacum, Satureja hortensis, Thymbra spicata, and Thymus vulgaris were used. The essential oils of these plants were obtained by the hydrodistillation method. Antimicrobial activities of the essential oils against microorganisms, including multiple antibiotic-resistant bacteria, were investigated using the disc diffusion method. All the essential oils used in this study were very effective against Gram-positive and Gram-negative bacteria, which included multiple resistant strains. All essential oils tested were especially very effective against Streptococcus pyogenes. The maximum antimicrobial activity was observed with the essential oils of S. hortensis. The antimicrobial activities of the essential oils varied depending on the species. In fact, the essential oils of some thyme species showed different levels of antimicrobial activities. The antioxidant activity results show that the percentage of inhibition values ranged from 28.32 to 94.55 %. Among essential oils, O. vulgare var. gracile oil showed the lowest antioxidant capacity (28.32 %), while S. hortensis showed the highest one (94.55 %). Generally, the data suggest that different thyme species oil possesses useful antimicrobial and antioxidant properties and may be utilized in the food industry and as a dietary supplement.Öğe Chemical Composition, Antimicrobial and Antioxidant Activities of Hyssop (Hyssopus officinalis L ) Essential Oil(Univ Agr Sci & Veterinary Med Cluj-Napoca, 2010) Kizil, Suleyman; Hasimi, Nesrin; Tolan, Veysel; Kilinc, Ersin; Karatas, HakanThe essential oil of hyssop is widely used in food pharmaceutical and cosmetic industries throughout the world Therefore, it is very important to know the chemical characteristics of the oil for economic use and enhanced performance of the end products This study was carried out to determine antimicrobial and antioxidant activities of the essential oil of Hyssopus officinalis (L) (Lamiaceae) collected from wild in the Southeast Anatolian Turkey Chemical compositions of hydrodistilled essential oils obtained from hyssop leaves were analyzed by gas chromatography mass spectrometry (GC MS) For antimicrobial activity disc diffusion tests were carried out on Escherichia coli line ATCC25922 Pseudomonas aeroginosa line ATCC27853 Staphylococcus aureus line 25923 Staphylococcus pyogenes line ATCC19615 and Candida albicans line ATCC10231 and the antioxidant activity was determined by using the diphenylpicrylhydrazyl (DPPH) radical scavenging method It was determined that hyssop essential oil contained isopinocamphone (5727%) () beta pinene (7 23%) () telpinen 4-ol (7 13%) pinocaivone (649%) carvacrol (3 02%) p cymene (2 81%) and myrtenal (2 32%) as major components As shown by treatments with 5 and 10 mu of oil which exhibited strong antimicrobial activity against S pyogenes S aureus C albicans and E colt but not against P aeruginosa The antioxidant activity of H officinalis essential oil was lower compared to butylated hydroxytoluene (BHT) and ascorbic acid These results demonstrated that hyssop essential oil has relatively low antioxidant activity and good antimicrobial activity against some test organismsÖğe Critical roles of SRC-3 in the development and progression of breast cancer, rendering it a prospective clinical target(MDPI, 31.10.2023) Varışli, Lokman; Dancik, Garrett M.; Tolan, Veysel; Vlahopoulos, SpirosBreast cancer (BCa) is the most frequently diagnosed malignant tumor in women and is also one of the leading causes of cancer-related death. Most breast tumors are hormone-dependent and estrogen signaling plays a critical role in promoting the survival and malignant behaviors of these cells. Estrogen signaling involves ligand-activated cytoplasmic estrogen receptors that translocate to the nucleus with various co-regulators, such as steroid receptor co-activator (SRC) family members, and bind to the promoters of target genes and regulate their expression. SRC-3 is a member of this family that interacts with, and enhances, the transcriptional activity of the ligand activated estrogen receptor. Although SRC-3 has important roles in normal homeostasis and developmental processes, it has been shown to be amplified and overexpressed in breast cancer and to promote malignancy. The malignancy-promoting potential of SRC-3 is diverse and involves both promoting malignant behavior of tumor cells and creating a tumor microenvironment that has an immunosuppressive phenotype. SRC-3 also inhibits the recruitment of tumor-infiltrating lymphocytes with effector function and promotes stemness. Furthermore, SRC-3 is also involved in the development of resistance to hormone therapy and immunotherapy during breast cancer treatment. The versatility of SRC-3 in promoting breast cancer malignancy in this way makes it a good target, and methodical targeting of SRC-3 probably will be important for the success of breast cancer treatment.Öğe Determination of Essential Oil Composition, Antimicrobial and Antioxidant Properties of Anise (Pimpinella anisum L.) and Cumin (Cuminum cyminum L.) Seeds(Ankara Univ, Fac Agr, 2014) Hasimi, Nesrin; Tolan, Veysel; Kizil, Suleyman; Kilinc, ErsinIn this research, the essential oil components, antimicrobial and antioxidant properties of anise (Pimpinella anisum L.) and cumin (Cuminum cyminum L.) seed oils were investigated. The essential oil components determined by GC/MS instrument. The antimicrobial activity determined by disc diffusion method against the gram negative bacteria, namely Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, the gram positive bacteria namely Staphylococcus aureus ATCC 25923, Streptococcus pyogenes ATCC19615 and Candida albicans ATCC10231 as the yeast. The antioxidant activities of essential oils were carried out by DPPH free radical scavenging activity method. It was determined that the main components of the anise essential oil were trans-anethole (52.94%) followed by iso-anethole (13.89%), caryophllene oxide (8.55%) and caryophyllene (29.4%); the main components of the cumin essential oil were beta-pinene (15.77%), alpha-terpinene (15:52%), 1-Phenyl-1-butanol (15:13%), cumic aldehyde (12.74%) respectively. Anise essential oil showed weak antimicrobial activity and cumin essential oil showed moderate antimicrobial activity against test microorganisms. Cumin essential oil showed strong antimicrobial activity against C. albicans with 22 +/- 0.9 mm inhibition zone diameter. P. aeruginosa showed resistance to both essential oils. The antioxidant activity of cumin essential oil (75.60%) was observed closer to ascorbic acid (78.75%), higher than BHA (50.45%) and BHT (23.54%). Although anise essential oil exhibited low activity, it has the similar activity with BHT. The scavenging activity of the samples decreasing order was ascorbic acid> cumin >BHA>BHT> anise.Öğe Dissecting the effects of androgen deprivation therapy on cadherin switching in advanced prostate cancer: A molecular perspective(Tech Science Press, 2022) Varışli, Lokman; Tolan, Veysel; Cen, Jiyan H.; Vlahopoulas, Spiros; Cen, OsmanProstate cancer is one of the most often diagnosed malignancies in males and its prevalence is rising in both developed and developing countries. Androgen deprivation therapy has been used as a standard treatment approach for advanced prostate cancer for more than 80 years. The primary aim of androgen deprivation therapy is to decrease circulatory androgen and block androgen signaling. Although a partly remediation is accomplished at the beginning of treatment, some cell populations become refractory to androgen deprivation therapy and continue to metastasize. Recent evidences suggest that androgen deprivation therapy may cause cadherin switching, from E-cadherin to N-cadherin, which is the hallmark of epithelial-mesenchymal transition. Diverse direct and indirect mechanisms are involved in this switching and consequently, the cadherin pool changes from E-cadherin to N-cadherin in the epithelial cells. Since E-cadherin represses invasive and migrative behaviors of the tumor cells, the loss of E-cadherin disrupts epithelial tissue structure leading to the release of tumor cells into surrounding tissues and circulation. In this study, we review the androgen deprivation therapy-dependent cadherin switching in advanced prostate cancer with emphasis on its molecular basis especially the transcriptional factors regulated through TFG-β pathway.Öğe Diyarbakır çevresinde yetişen endemik ajuga vestita ekstresinin sekonder metabolitlerinin ve bazı biyolojik aktivitelerinin belirlenmesi(2012) Haşimci, Nesrin; Kolak, Ufuk; Tolan, Veysel[Abstract Not Available]Öğe The effect of microwave on plasmids amplification and cell differentiation in bacillus subtilis(2005) Otludil, Birol; Tolan, Veysel; Otludil, Birgül Agüloğlu; Demir, R.; 0000-0002-3809-5987This study was carried out to investigate plasmid amplification and other differentiation by microwave irradiation (1 mV/cm2) and chloramphenicol treatment (170 ?g/ml) in Bacillus subtilis. The effect of microwave on B.subtilis was observed and the amounts of DNA, RNA, protein and plasmid were determined during the experiments. The data obtained were evaluated statistically and the variability of the amounts of DNA, Protein, RNA, Growth and plasmid exposed to microwave were less than those treated with chloramphenicol and these amounts significantly differ between microwave and chloramphenicol. Microwave exposure induces any broken fragment and the amounts of plasmids increased.Öğe The effect of microwave on the cellular differentiation bacillus subtilis yb 886 and rec derivatives yb 886 a4(2004) Otludil, Birol; Otludil, Birgül; Tolan, Veysel; Akbayın, HasanThis study was carried out to investigate effects of microwave irradiation on cell differentiation and SOS repair system in Bacillus subtilis rec+ YB 886 and rec derivatives YB 886 rec A4. Amount of a specific protein that shynthesed during DNA damage by SOS repair system and binding to din C promoter region increased by microwave irradiation in rec+ bacteria. More increasing were determined in amount of the specific protein during Ultraviolet treatment. If amount of DNA, protein and RNA are taken into consideration, it has observed that amount of DNA decreased (P>0.05) but not statically significant, amount of protein decreased (p<0.05) and a little increasing, (P>0.05) in amount of RNA may be due to structural deformation.Öğe The effects on extracellular and membrane in amylase production of the tetradentate schiff base, its mn(ii), ni(ii), cu(ii) and zn(ii) complexes and metal ions in bacillus subtilis(2005) Otludil, Birol; Otludil, Birgül Agüloğlu; Demir, R.; Tolan, Veysel; Temel, Hamdi; 0000-0001-9225-7425Bacillus subtilis was grown in mediums containing various schiff base (N, N'-Bis(salicylidene)-l,4-diaminobutane) that include transition metal complexes and metal ions. The effects of these substances on the production of secreted amylase, membrane bound amylase as well as the transport of amylase and bacterial growth were investigated. The results revelaed that the synthesis rate of amylase isolated from bacterial membranes was stimulated by growth mediums separately contains Mn(II) and Zn(II)complexes. In addition, Zn(II) complexes increased the transport amylase through the membrane, while Ni(II) and Cu(II) complexes inhibited it.Öğe Endosülfanın Bacillus subtilis'te üreme, plazmid ve α-amilaz sekresyonu üzerine etkisi(2018) Tolan, Veysel; Ensari, N. YavuzBu çalışmada Nottingham Üniversitesi Tıp Fakültesi Mikrobiyoloji Bölümü' nden temin edilen B. subtüis kullanıldı. Bakterinin hangi besi yerinde daha iyi üreme gösterdiği ve aktivite tayin yön temi ile bu ortamda a-amilaz sekresyonunun kaçıncı saatte maksimuma ulaştığı tespit edildi. Modifiye nişastalı LB sıvı besi yerinde pH ve CaCİ2 optimizasyonu yapıldıktan sonra bu besi yeri kullanıldı. Değişik konsantrasyonlardaki endosülfan ile etkileştirilip üretilen B. subtüis 'in üremesi ve a-amilaz aktivitesi ile plazmitler üzerine etkisi araştırıldı. Endosülfanın bakteri üremesi ve a-amilaz üretimi üzerine inhibisyon etkisi yaptığı, plazmitleri ise amplifiye edici olduğu saptandı. Endosülfanın a-amilaz sekresyonu üzerine etkisi elektroforez yöntemi ile de tespit edildi. Ayrıca, endosülfanın plazmit üzerine etkisi elektroforetik ve spektrofotometrik olarak incelendi. în vitro olarak endosülfanın a-amilazı etkilemediği saptandı.Öğe Essential Oil Components, Microelement Contents and Antioxidant Effects of Nepeta italica L. and Achillea filipendulina LAM.(Taylor & Francis Ltd, 2015) Hasimi, Nesrin; Kizil, Sueleyman; Tolan, VeyselThe genus Nepeta (Lamiaceae) and Achillea (Asteraceae) are widely distributed in Turkey. In this study, the essential oils of Nepeta italica L. and Achillea filipendulina LAM. collected from Dicle district in Diyarbakir province in the Southeast Anatolia and from Hakkari province in East Anatolia of Turkey, were obtained by hydrodistillation of the aerial parts. The essential oils from Nepeta italica and Achillea filipendulina obtained by hydrodistillation were analyzed by gas chromatography-mass spectrometry GC Clarus 600-MS Clarus 600 C (Perkin Elmer) and microelements were analyzed by Perkin Elmer Optima 2100 DV ICP OEMS, and also antioxidant activity analyzed by DPPH radical scavenging assay. Achillea filipendulina and Nepeta italica essential oils showed similar antioxidant activity with inhibition of 55.3 % and 51.3 %, respectively, which are higher than BHT synthetic antioxidant with 23 % inhibition.Öğe Evaluation of Antioxidant, Cholinesterase Inhibitory and Antimicrobial Properties of Mentha longifolia subsp noeana and Its Secondary Metabolites(Acg Publications, 2015) Ertas, Abdulselam; Goeren, Ahmet C.; Hasimi, Nesrin; Tolan, Veysel; Kolak, UfukThe aim of the present study was to determine the chemical structures of the isolated compounds, the essential oil and fatty acid compositions of Mentha longifolia subsp. noeana with their biological activities. Ursolic acid (1), uvaol (2), stigmast-5-ene-3 beta-yl formate (3), stigmast-5-en-3-one (4), beta-sitosterol (5), bis(2ethylhexyl) benzene-1,2-dicarboxylate (6), hexacosyl (E)-ferulate (7) and 5-hydroxy-6,7,3',4'-tetramethoxy flavone (8) were obtained from the aerial parts. The compounds (2-4, 6, 7) were isolated for the first time from a Mentha species. Palmitic acid (40.8%) was the major component of the non-polar fraction obtained from the petroleum ether extract. Pulegone (32.3%) was the main constituent of the essential oil which exhibited strong butyrylcholinesterase inhibitory activity (77.36 +/- 0.29%), moderate antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Candida albicans. The methanol extract showed 80% inhibition of lipid peroxidation, and the acetone extract possessed moderate DPPH free radical scavenging activity (60% inhibition) at 100 mu g/mL.Öğe Evaluation of genotoxic effects of C60 fullerene-?-Fe2O3 and multiWall carbon nanotubes-?-Fe2O3 nanoparticles(Kafkas Üniversitesi Veteriner Fakültesi, 2018) Demirci, Özlem; Haşimi, Nesrin; Kılınç, Ersin; Tolan, VeyselThe use of magnetic nanoparticles in nanomedicine applications has increased significantly in recent years. Genotoxic evaluation ofthe nanomaterials used for this purpose is therefore very important. In our study, the genotoxic effect of C60 fullerene-γ-Fe2O3 andmulti-wall carbon nanotubes-γ-Fe2O3 magnetic nanoparticles over a wide concentration range (0.1, 1.0, 5.0, 10.0, 25.0, 50.0, and 100.0µg/plate) was investigated using the Bacterial Reverse Mutation Test. These magnetic nanoparticles did not cause genetic damage toSalmonella typhimurium TA100 and TA98 in the presence and absence of metabolic activation. Due to the rapid increase in the presenceof nanoparticles in our daily lives, mutagenicity and toxicity data related to nanoparticles are quite valuable. For this reason, in vivo andin vitro studies that allow for effective evaluation of these compounds is of the utmost importance.Öğe Genotoxic assessments of Tigris river surface water with Salmonella mutagenicity and SOS-chromotests(Parlar Scientific Publications (P S P), 2007) Tolan, Veysel; Otludil, BirolTwo in vitro tests (Salmonella/microsome mutagenicity test and SOS chromotest), one for bacterial mutagenicity and one for primary DNA damage, were assayed to determine the genotoxic activity of surface water of Tigris river. In the Salmonella test, all water samples, presence and absence of S9 mix, showed mutagenic activity on Salmonella typhimurium TA 98 and TA 100. All samples tested with the SOS-chromotest were not found to induce primary DNA damage in E. coli PQ37. For each sample, the sensitivity of the tests indicated that SOS-chromotest is less sensitive than Salmonella assay.Öğe GENOTOXICITY OF ESSENTIAL OIL OF Thymbra spicata L. var spicata IN SALMONELLA/MICROSOME AND SOS CHROMOTEST(Parlar Scientific Publications (P S P), 2009) Tolan, Veysel; Kizil, Sueleyman; Ozdemir, Sadin; Demirci, OzlemEssential oils are concentrated, hydrophobic liquid containing, volatile aroma compounds from plants, which are called aromatic herbs or aromatic plants. Various essential oils and their monoterpenoid constituents have been widely used as fragrances in cosmetics, as flavouring food additives. and as scenting agents in a variety of household products. The present study was undertaken to investigate the mutagenic potential of essential oil of Thymbra spicata L. var spicata by Sahnonella/microsome assay TA98 and TA 100 tester strains, and also SOS chromotest with E.coli PQ37 strain, with and without addition of an S9 metabolic activation mixture. The essential oil of T spicata L. var spicata showed weakly mutagenic activity on both strains of Salmonella either without or with S9 mixture. No mutagenicity was found in the SOS chromotest either with or without S9 mixture. These results indicate a weak mutagenicity of the essential oil of Thymbra spicata in vitro.Öğe In vitro biological activities and fatty acid profiles of Pistacia terebinthus fruits and Pistacia khinjuk seeds(Taylor & Francis Ltd, 2015) Hacibekiroglu, Isil; Yilmaz, Pelin Koseoglu; Hasimi, Nesrin; Kilinc, Ersin; Tolan, Veysel; Kolak, UfukThis study reports in vitro anticholinesterase, antioxidant and antimicrobial effects of the n-hexane, dichloromethane, ethanol and ethanol-water extracts prepared from Pistacia terebinthus L. fruits and Pistacia khinjuk Stocks seeds as well as their total phenolic and flavonoid contents, and fatty acid compositions. Ethanol and ethanol-water extracts of both species exhibited higher anticholinesterase activity than galanthamine. Among ABTS, DPPH and CUPRAC assays, the highest antioxidant capacity of the extracts was found in the last one. P. terebinthus ethanol extract being rich in flavonoid content showed the best cupric reducing effect. All extracts possessed no antimicrobial activity. The main fatty acid in P. terebinthus fruits (52.52%) and P. khinjuk seeds (59.44%) was found to be oleic acid. Our results indicate that P. terebinthus fruits and P. khinjuk seeds could be a good source of anticholinesterase compounds, and could be phytochemically investigated.Öğe Increased ROS alters E-/N-cadherin levels and promotes migration in prostate cancer cells(Aepress, 2022) Varisli, Lokman; Tolan, VeyselBackground: Increased ROS has been reported to cause a change in E- and N-cadherin levels, and consequently promotes migrative behaviors in pancreas and breast cancer cells. In this study, the effect of a sublethal dose of H2O2 on E- and N-cadherin levels, and on migrative behaviors of PCa cells was investigated. Methods: To determine a sublethal concentration of H2O2 on cell proliferation and ROS production were examined using WST-1 and DCFH-DA assays, respectively. E- and N-cadherin protein and mRNA levels were investigated by western blotting and real-time PCR, respectively. The migrative potentials of the cells were examined by Cytoselect 96-well cell migration assay. Results: Treatment of the PCa cells with a sublethal dose of H2O2 results in a decrease in E-cadherin and an increase in N-cadherin levels, at both mRNA and protein levels. However, inhibition of ERK using PD98059 abolishes the effects of H2O2. In addition, the cells that were treated with H2O2 have gained further migrative abilities compared to control cells, and this ability was repressed when PD98059 was used together with H2O2. Conclusion: Increased ROS alters E- and N-cadherin levels in an ERK-dependent manner and thereby promotes the migrative abilities of PCa cells.
