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Öğe Assessment of Clonal Fidelity Between Selected Lentisk Genotypes (Pistacia lentiscus L.) by IRAP and Florescent-based AFLP(Springer, 2014) Suzerer, V.; Metin, O. Karakas; Koc, I.; Onay, A.; Kilinc, F.; Ciftci, Y. Ozden; Uncuoglu, A. Altinkut[Abstract Not Available]Öğe Assessment of Genetic Stability of In Vitro Micrografted and Propagated Almond cv. Texas(Springer, 2012) Suzerer, V.; Akdemir, H.; Yildirim, H.; Onay, A.; Ciftci, Y. Ozden[Abstract Not Available]Öğe Genetic Instability of Long-term Micropropagated Mature Pistachio(Springer, 2014) Akdemir, H.; Suzerer, V.; Tilkat, E.; Onay, A.; Ciftci, Y. Ozden[Abstract Not Available]Öğe An Improved Micropropagation Protocol for Pistacia lentiscus(Springer, 2013) Yildirim, L. H.; Suzerer, V.; Onay, A.; Ciftci, Y. Ozden; Tilkat, E.; Koc, I.; Akdemir, O. F.[Abstract Not Available]Öğe Improved Shoot Multiplication of Lentisk (Pistacia lentiscus L.) Using Different Carbohydrates and Media(Int Soc Horticultural Science, 2014) Kilinc, F. M.; Suzerer, V.; Ciftci, Y. Ozden; Koc, I.; Akdemir, H.; Yildirim, H.; Tilkat, E.The aim of this study is to determine the effects of four cloned genotypes on efficient in vitro proliferation procedure by assessing the influence of different carbohydrates and medium types and their strengths to reveal whether they stimulate or inhibit shoot multiplication. Shoot multiplication was generally optimum on a modified full-length MS medium supplemented with 1 mg/L BA with subculture at 4-week intervals for all four tested clones. Shoot tips grown on 3% fructose medium produced more and longer shoots than those on sucrose, lactose or glucose in the clone 2. In this treatment, the maximum number of shoots per explant (3.62) was recorded on MS medium containing 1 mg/L BA. The maximum shoot proliferation (100%) together with the highest number of shoots per explant (3.60) was recorded on 1/2x MS medium containing 1 mg/L BA. In this context, the result of the clone 2 was superior to those of the rest of the clones tested. In vitro propagated microshoots were transferred to 1 mg/L IBA containing MS medium for rooting and acclimatized to in vivo conditions. This is the first report on the effect of carbohydrates and medium types and their strength on shoot multiplication and provides a basis for further research in the improvement of lentisk micropropagation.Öğe In Vitro Micrografting of Mastic Tree, Pistacia lentiscus L.(Springer, 2014) Suzerer, V.; Onay, A.; Kilinc, F.; Ciftci, Y. Ozden; Uncuoglu, A. Altinkut; Akdemir, H.; Tilkat, E.[Abstract Not Available]Öğe Micrografting of almond (Prunus dulcis Mill.) cultivars Ferragnes and Ferraduel(Elsevier, 2010) Yildirim, H.; Onay, A.; Suzerer, V.; Tilkat, E.; Ozden-Tokatli, Y.; Akdemir, H.The success of various in vitro micrografting techniques, establishment of the rootstock, size of the microscion, and the effects of culture medium on the grafted seedling development for almond cultivars Ferragnes and Ferraduel were studied. In vitro germinated wild almond seedlings developed from seeds were used as rootstocks. Shoot culture initiation was successfully achieved from the above almond cultivars by culturing mature shoot tips from forced nodal buds, about 3-5 mm. on 0.7 mg/L BA and 0.01 mg/L NAA containing a MS medium. The regenerated adventitious shoots from in vitro cultures were maintained and proliferated by sub-culturing on a fresh medium every three to 4 weeks. Regenerated shoot tips, which were micrografted onto in vitro seedlings, resulted in the restoration of shoot proliferation. The results indicated that the most successful method for the grafting of tested almond cultivars was slit micrografting. High levels of micrograft take were achieved with all ranges of scions (4-15 mm) obtained from the regenerated shoot tips. Slow growth and lack of axillary shoot development on the micrografts were noticeable when the micrografts were cultured on hormone-free germination medium. In vitro micrografted planners were successfully acclimatized and no problems were encountered with the establishment of micrografted plants in vivo. The developed technique has demonstrated a high potential for application in the micropropagation of almond cvs. Ferragnes and Ferraduel and thereby, represents a feasible method for the renewal of almond orchards in Turkey and elsewhere in the world. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.Öğe Micropropagation of Mature Khinjuk Pistachio, Pistacia khinjuk Stocks.(Springer, 2015) Ersali, Y.; Suzerer, V.; Tilkat, E.; Onay, A.; Uncuoglu, A. A.; Ciftci, Y. O.; Kilinc, F. M.[Abstract Not Available]Öğe Micropropagation of Pistachio: The Wave of the Future?(Int Soc Horticultural Science, 2011) Onay, A.; Suzerer, V.; Yildirim, H.; Tilkat, E.; Akdemir, H.; Ozden-Tokatli, Y.The aim of this review is concerned with the novel in vitro methods published for micropropagation and conservation of pistachio. Since the first publication by Barghchi in 1982, there have been considerable biotechnological advances in both micropropagation and conservation of pistachio germplasm. All of those studies revealed that the success of biotechnological approaches is dependent on regeneration of mature pistachio trees following successful culture initiation, generally by micropropagation. This review is focused upon the novel in vitro methods published for micropropagation and conservation of pistachio in which contributions of Barghchi, Abousalim, Onay, Parfitt, Tilkat and Ozden-Tokatli and others are discussed in a historical perspective. Examples of all aspects of micropropagation (shoot tip culture, somatic embryogenesis and adventive organogenesis) and currently employed uses of pistachio tissue culture, especially practical applications of micropropagation, which enable the pistachio industry to transition into an evaluation of what lies ahead, are also presented. Our work of over 20 years of experience will be presented in relation to past accomplishments, while presenting newer technologies and developments that may provide researchers with knowledge allowing them to determine the practicality and economic validity for micropropagation to become the wave of the future for large-scale commercial propagation of pistachios.Öğe Plant Production through Adventive Organogenesis of Mature Pistachio, Pistacia vera L. Cultivars 'Atli' and 'Siirt'(Int Soc Horticultural Science, 2011) Tilkat, E.; Akdemir, H.; Ozden-Tokatli, Y.; Yildirim, H.; Suzerer, V.; Onay, A.A method was developed for direct plant regeneration from in vitro-derived leaf explants of Pistacia vera L. cvs. Siirt and Atli. The in vitro adventive organogenesis involved six steps that included (1) explant disinfection, (2) the addition of cytokinins (benzyladenine; BA, Kinetin; Kin) for the in vitro installation of mature shoot tips, and the successful rapid proliferation of shoot initials, (3) induction of shoot initials from the regenerated mature leaves, (4) regeneration and elongation of shoots from the shoot initials, (5) rooting of the shoots and (6) acclimatization of the plantlets. Exudation of phenolics was not observed when the rinsed shoot tips were washed twice, in sterile distilled water, on a shaker at 150 rpm for 1 hour. Explants on the Murashige & Skoog (MS) medium with Gamborg vitamins supplemented with 1 mg L-1 BA produced the highest number of shoots and longest shoots in the second step. Leaves excised from axenic shoot cultures of pistachio were used to induce adventive organogenesis on a MS medium with Gamborg vitamins supplemented with combinations of different concentrations and combination of BA and indole-3-acetic acid (IAA). In the third stage, the best medium for shoot induction in both cultivars was MS medium with 1 mg L-1 IAA and 2 mg L-1 BA. For shoot multiplication, the highest number of new microshoot/ explants for male and female was obtained in a culture medium supplemented with 1 mg L-1 BA, but it was not significantly different from the number obtained at 2 mg L-1 BA in the fourth step. The elongated shoots (>4 cm long) were rooted on a full-strength MS basal medium supplemented with 2 mg L-1 of indole-3-butyric acid (IBA) in the fifth step. Finally, the rooted plants were transferred to soil. This protocol could be utilized for in vitro clonal propagation of this economically important plant.Öğe Quantitation of Total Oil Contents, Proteins and Fatty Acids Composition in Fruits of Pistacia Species and Their Hybrids Growing in Turkey.(Springer, 2015) Suzerer, V.; Onay, A.; Kizmaz, V.; Calar, N.; Uncuoglu, A. A.; Boukeloua, A.; Ilikcioglu, E.[Abstract Not Available]Öğe Somatic Embryogenesis in Pistachio(Int Soc Horticultural Science, 2011) Onay, A.; Suzerer, V.; Yildiriim, H.; Tilkat, E.; Akdemir, H.; Ozden-Tokatli, Y.Somatic embryogenesis and plant regeneration are fundamental to improve tissue culture biotechnology in pistachio (Pistacia vera L.). Hence, this study summarizes the research carried out in pistachio for induction of somatic embryogenesis together with discussing the current status of this technique in pistachio micropropagation. Moreover, it gives a contemporary interpretation of the process of the somatic embryogenesis. Attention has been focused on the influence of factors such as genotype, explant type, media composition, growth regulators, carbohydrate nutritions and treatment periods and interactions of some of these factors on the induction of somatic embryogenesis, maintenance of embryogenic cultures, development, maturation and germination of somatic embryos as well as transplantation of somatic seedlings into in vivo conditions. Although induction, development, maturation and germination of somatic embryos is being controlled to some extent in pistachio, high frequency regeneration, synchronous development of embryos and transplantation to the field were not very successful. As optimisation of an efficient tissue culture and plant regeneration protocol for pistachio is the first step towards the application of transgenic technology to improve pistachio breeding, further studies should be carried out initially to enhance frequency of regeneration from somatic embryos.
