Improved Shoot Multiplication of Lentisk (Pistacia lentiscus L.) Using Different Carbohydrates and Media
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Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Int Soc Horticultural Science
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The aim of this study is to determine the effects of four cloned genotypes on efficient in vitro proliferation procedure by assessing the influence of different carbohydrates and medium types and their strengths to reveal whether they stimulate or inhibit shoot multiplication. Shoot multiplication was generally optimum on a modified full-length MS medium supplemented with 1 mg/L BA with subculture at 4-week intervals for all four tested clones. Shoot tips grown on 3% fructose medium produced more and longer shoots than those on sucrose, lactose or glucose in the clone 2. In this treatment, the maximum number of shoots per explant (3.62) was recorded on MS medium containing 1 mg/L BA. The maximum shoot proliferation (100%) together with the highest number of shoots per explant (3.60) was recorded on 1/2x MS medium containing 1 mg/L BA. In this context, the result of the clone 2 was superior to those of the rest of the clones tested. In vitro propagated microshoots were transferred to 1 mg/L IBA containing MS medium for rooting and acclimatized to in vivo conditions. This is the first report on the effect of carbohydrates and medium types and their strength on shoot multiplication and provides a basis for further research in the improvement of lentisk micropropagation.
Açıklama
6th International Symposium on Almonds and Pistachios -- MAY 27-31, 2013 -- Murcia, SPAIN
Anahtar Kelimeler
Clone, Micropropagation, Medium, Mastic Tree, Carbone Source
Kaynak
Vi International Symposium on Almonds and Pistachios
WoS Q Değeri
N/A
Scopus Q Değeri
Q4
Cilt
1028
