Yazar "Olmez, Ozge Tokul" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • [ X ]
    Öğe
    Characterization of the Chemical Profile of Euphorbia Species from Turkey by Gas Chromatography-Mass Spectrometry (GC-MS), Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS), and Liquid Chromatography-Ion Trap-Time-of-Flight-Mass Spectrometry (LC-IT-TOF-MS) and Chemometric Analysis
    (Taylor & Francis Inc, 2019) Yener, Ismail; Ertas, Abdulselam; Yilmaz, Mustafa Abdullah; Olmez, Ozge Tokul; Yilmaz, Pelin Koseoglu; Yesil, Yeter; Kaplaner, Erhan
    The Euphorbiaceae family comprises of about 300 genera and 5000 species primarily distributed in America and tropical Africa. The Euphorbia genus is represented by 105 species and locally named as Sutlegen and Xasil in Turkey. The present study aimed to determine the chemical constituents of E. aleppica, E. eriophora, E. macroclada, E. grisophylla, E. seguieriana subsp. seguieriana, E. craspedia, E. denticulata, E. falcata, and E. fistulosa, and classify them by utilizing the chemometric techniques of principal component analysis (PCA) and hierarchical cluster analysis (HCA). Linoleic acid, 17-tetratriacontane, palmitic acid, and hexatriacontane were the major fatty acids from the gas chromatography-mass spectrometry (GC/MS) analyses. Characterization of 268 constituents of the studied species was achieved by liquid chromatography-ion trap-time-of-flight-mass spectrometry (LC-IT-TOF-MS). Furthermore, a new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous quantitative determination of 11 compounds (quinic acid, protocatechuic acid, rutin, hesperidin, eugenol, p-coumaric acid, piceatannol, scopoletin, dl-kavain, chrysophanic acid, and resiniferatoxin) in these species. The developed method was validated for the linearity, limit of detection, limit of quantification, repeatability, and recovery.
  • [ X ]
    Öğe
    A detailed study on chemical and biological profile of nine Euphorbia species from Turkey with chemometric approach: Remarkable cytotoxicity of E-fistulasa and promising tannic acid content of E-eriophora
    (Elsevier Science Bv, 2018) Yener, Ismail; Olmez, Ozge Tokul; Ertas, Abdulselam; Yilmaz, Mustafa Abdullah; Firat, Mehmet; Kandemir, Sevgi Irtegun; Ozturk, Mehmet
    The propose of this study is to investigate the chemical constituents of nine Euphorbia species; namely, E. aleppica, E. eriophora, E. macroclada, E. grisophylla, E. seguieriana subsp. seguieriana, E. craspedia, E. denticulata, E. falcata and E. fistulosa by LC-MS/MS as well as their antioxidant, anticholinesterase, tyrosinase inhibitory, urease inhibitory and cytotoxic activities. Among 59 extracts prepared from the different parts of the mentioned Euphorbia species, E. seguieriana subsp. seguieriana leaf (IC50: 10.41 +/- 0.93 mu g/mL) in lipid peroxidation inhibitory by beta-carotene-linoleic acid assay exhibited the highest activity, while E. grisophylla root in DPPH free radical scavenging (IC50: 0.79 +/- 0.01 mu g/mL), E. grisophylla seed in ABTS cation radical scavenging (IC50: 8.93 +/- 0.02 mu g/mL), E. fistulosa root both in acetyl- and butyryl-cholinesterase inhibitory (53.71 +/- 1.89% and 77.63 +/- 0.60% inhibition, respectively), E. denticulata leaf in urease inhibitory (96.89 +/- 2.00% inhibition), and E. macroclada mixed in tyrosinase inhibitory (86.99 +/- 3.77% inhibition) activities. However in cytotoxic activity studies, E. denticulata against PDF fibroblast cell lines (IC50: 23.04 +/- 0.03 mu g/mL), E. craspedia against HT-29 cancer cell line (IC50: 13.74 +/- 0.02 mu g/mL), E. fistulosa against MCF-7 and DLD-1 cancer cell lines (IC50: 14.04 +/- 0.04 mu g/mL and IC50: 20.23 +/- 0.08 mu g/mL, respectively) indicated quite good activity. According to the LC-MS/MS results, these species were rich in quinic acid, malic acid and tannic acid, rutin, hesperidin and hyperoside. Incidentally, it was found that the E. eriophora possessed very strong irritating potential. A chemometric approach using principal component analysis (PCA) and hierarchical clustering analysis (HCA) techniques were also studied on collected data to reveal the relationship between chemical contents of different parts of these Euphorbia species, and their biological activities, together with regional differences. Overall, these species could be suggested as valuable sources of natural-bioactive agents for developing new functional, pharmacological and health promoting ingredients.