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Öğe A congenital cystic adenomatoid malformation case(1991) Aydin G.; Ariturk E.; Dokucu A.I.; Nergiz Y.[No abstract available]Öğe The demonstration of changes in bulbar conjunctiva surface epithelium in the psoriatic patients treated with PUVA.(2008) Söker S.; Nergiz Y.; Cakmak S.; Bahçeci S.; Aytekin S.We investigated the effects of disease itself and PUVA treatment on surface epithelium of conjunctiva in psoriatic patients (PP) before PUVA and after PUVA therapy and in 32 healthy volunteers. Squamous metaplasia was detected in PP both before and after PUVA therapy. We concluded that PUVA treatment applied together with preventive measures, would lead to less severe ocular side effects.Öğe The effect of valproic acid on rat ovarium and the protective role of vitamin E and folic acid: An ultrastructural study(2010) Aktaş A.; Nergiz Y.; Akkuş M.This study was undertaken to investigate the effect of valproic acid and protective effects of vitamin E and folic acid on rat ovary ultrastructural changes. Twenty-four Wistar rats were used. Animals were divided into four groups. The first group of rats was used as control. The second group was injected valproic acid. The third group was injected valproic acid + folic acid and the fourth group was given valproic acid + vitamin E. At the end of the study, ovarium tissues were taken under anesthesia. Tissues were prepared and examined by transmission electron microscopy. Microscopically, the groups are cited as follows: Control group (in which the ovarium tissue was normal), valproic acid group (which showed increase in lipid content plus mitochondrial crystalysis seen in folliculer and theca interna cells of rat ovarium), valproic acid + folic acid group (in which the theca interna and granulosa cells of rat ovarium had normal appearance) and valproic acid + vitamin E group (where all the organelles of theca interna and granulosa cells of rat ovarium were observed to be normal). Vitamin E and folic acid have protective effects against valproic acid-induced tissue damage in rat ovaries. © 2010 Academic Journals.Öğe Effects of genistein, estrogen and progesterone therapies on bladder morphology and M2, M3 receptor expressions in oophorectomized rats(Acta Medica Mediterranea, 2014) Turgut A.; Goruk N.Y.; Sak M.E.; Deveci E.; Akdemir F.; Keles A.N.; Nergiz Y.Aims: Investigating the effects of estrogen, estrogen/progesterone combination and genistein therapy on the expression of M2 and M3 receptors located on bladder walls and comparing the morphological and degenerative changes exerted on bladder walls. Materials and methods: A total of 50 adult Sprague-Dawley female rats were randomly divided into five groups. Rats other than the sham group were ovariectomized. OVX group (control group) received water, OVX+G group received 10 mg/kg genistein, OVX+E group received 0.014 mg/kg 17-ß estradiol, OVX+E+P group received 0.014 mg/kg 17-ß estradiol plus 0.028 mg/kg drospirenone per day. Results: When compared with the sham group, in the OVX group higher collagen fibre (CF): smooth muscle (SM) ratio, relatively increased fibrosis, oedema, space between detrusor smooth muscle fascicles, cytoplasmic vacuoles, and total M2, and M3 expression were observed. Relative to the OVX group, decreased CF: SM ratio and fibrosis in the OVX+G, OVX+E, and OVX+E+P groups, decreased oedema, spaces between detrusor muscle fascicles and cytoplasmic vacuoles in the OVX+G group and lesser total M2, and M3 expression in the OVX+G, OVX+E and OVX+E+P groups were observed. Conclusion: Genistein therapy regresses unfavourable morphological changes effecting postmenopausal bladder and increases in M2 and M3 receptor expression more effectively than estrogen and estrogen/progesterone combination. Besides, genistein therapy almost completely regresses degenerative changes; however, estrogen and estrogen/progesterone combination therapies do not improve these degenerative changes except for fibrosis. We think that genistein will favourably contribute both to the conduction of more comprehensive studies in the future concerning its use in postmenopausal urinary incontinence where estrogen and estrogen/progesterone combination therapies do not provide any improvement and etiopathogenesis of urinary incontinence.Öğe The effects of pulsed and sinusoidal electromagnetic fields on E-cadherin and type IV collagen in gingiva: A histopathological and immunohistochemical study(Wroclaw University of Medicine, 2013) Dogru A.G.; Tunik S.; Akpolat V.; Dogru M.; Saribas E.E.; Kaya F.A.; Nergiz Y.Background. The potential beneficial effects of extremely low frequency pulsed and sinusoidal electromagnetic fields have been shown on many tissues. Gingival epithelium plays an important role in immunosurveillance of the periodontal tissues. The epithelium acts as a mechanical barrier through cell junctions such as E-cadherin. Objectives. Investigation of the effects of extremely low frequency magnetic fields on gingiva. Material and Methods. Twenty-seven male Wistar albino rats were used. The rats were divided into three groups: control group (n = 9), SEMF group (n = 9), PEMF group (n = 9). The SEMF and PEMF (pulse time: 25 ?sn, pulse frequency: 50 Hz) groups were subjected to 1.5 mT, 50 Hz, exposure 6 h a day, 5 days a week for 28 days in methacrylate boxes. The gingival tissue pieces processed for routine histological and immunohistochemical examination and tissue sections were stained with H-E and Masson trichrome. In addition, E-cadherin and type IV collagen expressions were examined immunohistochemically. Results. Intraepithelial lymphocytes and proliferation of epithelial cells increased in both electromagnetic field groups. The over-expressions of E-cadherin on gingival epithelium was detected in the PEMF and SEMF groups. The expression level of type IV collagen was not significant between the control and electromagnetic field treated groups, except for a significant increase in the basal cell layer of the PEMF group, as compared to the control and SEMF groups. Conclusions. PEMF and SEMF have a local pro-inflammatory effect on gingiva, leading to an increase in E-cadherin level but not type IV collagen. Both PEMF and SEMF can be used as a supportive device in the treatment of gingival diseases, especially those which lead to defects in the epithelial barrier. © Copyright by Wroclaw Medical University.Öğe An experimental Staphylococcus aureus meningitis model for investigating induced leptomeningeal and subpial inflammation in rats: A transmission electron microscopy study(Maghira and Maas Publications, 2007) Guzel A.; Er U.; Tatli M.; Aluclu U.; Ozekinci T.; Nergiz Y.; Ahishali B.Objective: To evaluate leptomeningeal and subpial inflammatory responses of experimental Staphylococcus aureus bacteriemia following intraperitoneal and intravenous applications and to compare the inflammatory reactions in different regions of central nervous-system. Material and Methods: Forty anesthetized rats were divided into four groups equal in number. The rats in group-I were given 1 ml suspension of Staphylococcus aureus intraperitoneally. Group-II was the control group of group I; it was administrated 1 ml 0.9% NaCl in water intraperitoneally. The rats in group-III were given the same amount of bacteria intravenously. Group IV was the control group of the group-III; it was administrated 1 ml 0.9% NaCl solution intravenously. The rats were sacrificed on the 21st day. Inflammatory changes of different regions of the central nervous system were examined under transmission electron microscopy. Statistical analysis was done by using variance analysis, Bonferroni, Tamhane post hoc, Student's t and univariate tests. Results: Thoracic and occipital regions were the most vulnerable zones. Increasing of collagen tissue was the most detected inflammatory change. Conclusion: This experimental model can be used for inducing subpial and leptomeningeal inflammations and it may be developed for investigations of pathogenesis of leptomeningitis during systemic infections. © 2007 Neuroendocrinology Letters.Öğe Gingival metastase and intracerebral haemorrhage resulting from unsuspected choriocarcinoma: A case report(2010) Tasdemir M.S.; Aktas A.; Tasdemir N.; Nergiz Y.Gestational choriocarcinoma is a higly malignant epithelial tumour arising from the trophoblasts of any type of gestational event, most often a hydatidiform mole. Choriocarcinoma is for all practical purposes limited to reproductive age women but rare examples of choriocarcinoma in postmenoupausal women have been reported. In case of a 24- years- old was a female patient with left hemiparalysis. Biopsy that obtained from lower gingival ulcer indicated the availability of choriocarcinoma metastasis. Before putting her in our intensive care unit she suddenly became unconscious. Focally haemorrhagic fragile gingival tissue (grossly, 1× 1× 0.5 cm) was processed. In the formaline fixed, paraffin embedded and hematoxylin and eosin(H&E) stained slides, beneath the stratified squamous epithelia, there was tumoral infiltration. Tumor was consisted of abnormal cytotrophoblasts and syncytiotrophoblasts cells and diffuse intercerebral haemorrhage. In this report we presented a rare case of metastatic gingival choriocarcinoma and intracranial haemorrhage resulting from an unsuspected choriocarcinoma metastasis.Öğe The histological structure of filiform and fungiform papillae of the puppy rats which born from the rats given EGF after sialoadenectomy(2009) Aktas A.; Nergiz Y.After the complete removal of submandibular gland surgically, morphological alterations, and the effects of EGF given orally on these alterations were investigated. In the study, 30 adult female Spraque-Dawley rats were used, which were divided equally into three groups: Control group; sialoadenectomy group; sialoadenectomy + epidermal growth factor group. Following a three week period of recovery, the rats were mated. On days 16-19 th of pregnancy, a total of 5 microgram EGF was given to the animals in sialoadenectomy + epidermal growth factor group with orogastric tube as 1.25?g daily to each animal. The fetuses born following pregnancy were kept to grow up until the 28th day. The pupy rat of 28 days in all groups were taken, and then they were sacrificed. Tongues of all puppy rats were fixed in a 10 % neutral buffered formalin solution. The paraffin sections obtained through routine histological methods were stained with Hematoxylen-Eosine, Methylen blue- Basic Fuchsin, Hemotoxylen-Van Giesson, and were examined under light microscope. A significant increase in the keratinization; an elongation and bifurcation at the ends of the filiform papillae of the sialoadenectomy group rats were observed. The fungiform papilla and the taste buds were noticed to be larger than that of the control group, and a decrease was detected in the cells population that constitutes the taste buds. Bifurcation and hyperkeratosis in the filiform papillae of the sialoadenectomy + epidermal growth factor group rats.The morphological appearance of fungiform papillae and taste buds was observed to be similar to that of fungiform papillae of the control group.Öğe Histopathologic effect of gonadal steroids and gender on goiter development in thyroid stimulated rabbits by TRH(1995) Aydin I.; Nergiz Y.; Ketani A.; Aslan I.In order to assess the effect of gender and gonadal steroids on goiter development, thirty-two New Zealand albino rabbits were studied. The experimental protocol was based on histopathological changes observed in the thyroid gland following manipulation of gonadal hormone levels and stimulation by TRH. Gender differences for the structure end size of follicles following TRH administration remained significantly larger in females than in males (p < 0.001). In gonadectomied male and female groups, there appeared to be a quantitative difference in response to TRH. Follicular cell proliferation and papillary structures were prominent and the diameter of the follicles was significantly larger in males compared with females (P < 0.001). In gonadectomied rabbits treated with the opposite gonadal steroids, the appearance of papillary structures and basophilic character of the colloid on staining were also prominent in males. The results indicate that (i) estrogen promotes while androgen inhibits to some extent the stimulatory effects of TRH on thyroid follicular size; and (ii) although the data would support the effect of gender and sex steroids on goiter development, factors other than gonadal steroids are responsible for the higher incidence of goiter in females.Öğe Intravesical oxybutynin application: Ultrastructural effects on bladder epithelium(1999) Ersay A.; Ketani M.A.; Nergiz Y.; Demirtas O.C.; Akkus M.; Otludil B.Intravesically applied oxybutynin, rapidly absorbs into the bloodstream, additional to profound local effect. Currently morphologic effects of oxybutynin on local bladder tissue relatively well established at light microscopic level, but not ultrastructural level. Thirty New Zealand White female rabbits were catheterized daily and intravesical instillation were performed with whether l mg/kg oxybutynin solution or saline for 30 days. The local effects of the drug on bladder epithelium at electron microscopic level were examined comparing with saline administration. Urinary tract infection (UTI) incidence were similar in both saline and oxybutynin groups (9 vs. 10 of 15 animals respectively) (p>0.05). Interestingly, in 4 of 5 animals that received oxybutynin and never had UTI during the study, separation of zonula occludens intercellular junction was determinated by Jeol electron microscope. This observation can explain rapid absorption of oxybutynin through the bladder into the blood stream. Oxybutynin can damage bladder surface epithelium at ultrastructural level. This effect may lead to increase absorption of it, but not result in higher incidence of bacterial infection.Öğe Investigation of functional sperm membrane integrity after consequence of anti–hypoosmotic swelling test technique: An electron microscopic study(Journal of Reproductive Medicine, Inc., 2015) Tutsi Y.; Akkus M.; Tunik S.; Nergiz Y.; Kaplanoglu I.; Nasir Y.OBJECTIVE: Infertility is described as the inability of a couple to conceive after 1 year of unprotected intercourse. Male factor is assumed to be responsible for 50% of cases of infertile couples. It is a common clinical problem, affecting approximately 13– 15% of couples worldwide. In this study we aimed to investigate the effects of hypoosmotic swelling (HOS) and anti-HOS tests on sperm membrane integrity at the ultrastructural level. STUDY DESIGN: Twenty-nine infertile and 10 fertile men were included in this study. The fertile and infertile subjects were classified according to Kruger and WHO criterion. All semen samples were examined and analyzed based on WHO guidelines. Sperm viability was determined by using the eosin Y staining method. After HOS and anti-HOS tests were applied, the samples were evaluated at the ultrastructural level. RESULTS: Normal structural features of all regions of sperm were observed in sections of sham normospermia. Some histopathological changes were seen in HOS and anti-HOS group sections. CONCLUSION: The HOS procedure was found not to cause degenerative changes in the sperm ultrastructure. The anti-HOS procedure can be applied in normospermic and oligospermic groups. © Journal of Reproductive Medicine®, Inc.Öğe Light microscope evaluation of iron accumulation in term placentas of subjects with preeclampsia(Acta Medica Mediterranea, 2014) Nasir Y.; Nergiz Y.; Aktaş A.; Akdeniz N.Aims: In this study, the aim was to investigate if the the use of placental iron storages detection could be used. Materials and Methods: This study was performed on 30 placentas, 20 preeclamptic and 10 normal gestations, of 36 weeks or more. Paraffin sections of preeclamptic and normotensive placental tissues were stained with Hematoxylin and Eosin, Masson's Trichrome, and Prussian Blue. Results: Evaluation of preeclampsia group placentas showed as most common findings: increased syncytial node in villus, cytotrophoblast proliferation, increased volume and number of fetal capillary, and increased fibrinoid accumulation. Among preeclampsia group placental tissue samples, Prussian Blue staining was observed most commonly in fetal central cross-sections. In most prominently Prussian Blue stained villus, presence of syncytial nodes and close neighboring between iron accumulation and syncytial kodes ere interesting findings. Statistical analysis of data was made by SPSS software using Mann Whitney U test and p values under 0.05 ere accepted as statistically significant. Conclusion: Studies of placental light microscopy are important in the clarification of preeclampsia etiopathogenesis and more studies with wider scope are needed.Öğe Progressive external ophthalmoplegia (PEO): Presentation of a mitochondrial myopathy accompanied by electron microscope(2010) Aktas A.; Tasdemir M.S.; Tasdemir N.; Nergiz Y.Kearns-Sayre syndrome is a mitochondrial disease, presenting findings before the age of 20 and characterized by chronic progressive external ophthalmoplegia and pigmentary retinal degeneration. It affects many organs, resulting in a very wide spectrum of complications. In this work, a 24-year-old female, whose complaints first started at the age of 12, showing progressing external ophthalmoplegia and diagnosed with Kearns-Sayre disease following mitochondrial changes in muscle electron microscope investigation was presented. Ways of diagnosing in mitochondrial diseases, especially those in children were tried to be evaluated.
